Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,938,8870CT28.4% 532.7 / 170.5 271S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (19/175);  new base T (77/0);  total (96/175)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 317 total aligned reads displayed.

ACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAC  >  AP012306/3938738‑3939035
                                                                                                                                                     |                                                                                                                                                     
     tAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttttt                                                                                                                                                  <  2:328829/150‑1 (MQ=255)
       gAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttttt                                                                                                                                                >  2:3899951/1‑148 (MQ=255)
         aaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttttttt                                                                                                                                              >  2:1688890/1‑146 (MQ=255)
         aaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttttttc                                                                                                                                              >  1:2767869/1‑146 (MQ=255)
          aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCCCCATACCTATACCAGTCAGGATTCATTTCAGGGTGCCATGTCGCGGATCTCGTTCGCGGCGGCTATTttttttttctctcgc                                                                                                                                             >  2:3948438/1‑147 (MQ=255)
            aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCAt                                                                                                                                           <  2:2439757/150‑1 (MQ=255)
               cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgag                                                                                                                                        <  2:1748403/150‑1 (MQ=255)
                 tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGaa                                                                                                                                      <  2:1107776/150‑1 (MQ=255)
                   aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGt                                                                                                                                    <  1:521402/150‑1 (MQ=255)
                     gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGa                                                                                                                                  <  2:304798/150‑1 (MQ=255)
                       gtagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGGgc                                                                                                                                >  1:1239447/1‑150 (MQ=255)
                        tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCATGAGAAGTGAgcg                                                                                                                               >  2:3446638/1‑150 (MQ=255)
                        tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                               <  2:1982978/150‑1 (MQ=255)
                        tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                               <  2:2492063/150‑1 (MQ=255)
                        tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                               <  1:4070286/150‑1 (MQ=255)
                          gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCa                                                                                                                             <  1:2434821/150‑1 (MQ=255)
                            aTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCGTTTTTTTTTTTTTTTGCATGAGAAGTGAgggggga                                                                                                                           >  2:575336/1‑144 (MQ=255)
                             tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                                          <  1:130554/150‑1 (MQ=255)
                             tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                                          <  1:3455046/150‑1 (MQ=255)
                             tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                                          <  1:2561996/150‑1 (MQ=255)
                             tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                                          <  1:3254012/150‑1 (MQ=255)
                              cGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga                                                                                                                         <  2:3570982/150‑1 (MQ=255)
                               ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagagag                                                                                                                        <  2:1465712/150‑1 (MQ=255)
                               ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagagag                                                                                                                        <  1:10754/150‑1 (MQ=255)
                                 tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCCTGTGAAGTGAGCGGCGAGAagg                                                                                                                      >  2:4084211/1‑150 (MQ=255)
                                 tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                                      <  1:3172837/150‑1 (MQ=255)
                                  aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGa                                                                                                                     <  2:3861736/150‑1 (MQ=255)
                                   ttGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCACTTTTTTTTTTTCTCCTCTGAGAAGTAAGCGGAAAGAggggt                                                                                                                    >  2:316313/1‑148 (MQ=255)
                                    tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGAtt                                                                                                                   <  1:1986313/150‑1 (MQ=255)
                                     gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTa                                                                                                                  <  2:2575946/150‑1 (MQ=255)
                                      agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTAt                                                                                                                 <  1:93976/150‑1 (MQ=255)
                                      agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTAt                                                                                                                 <  2:2308856/150‑1 (MQ=255)
                                      agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTAt                                                                                                                 <  1:421226/150‑1 (MQ=255)
                                          tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCttt                                                                                                             >  1:571811/1‑149 (MQ=255)
                                          tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTg                                                                                                             <  1:1004288/150‑1 (MQ=255)
                                           ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCAGAGGGGGGttttttttc                                                                                                            >  2:2867343/1‑143 (MQ=255)
                                            tATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGcc                                                                                                           <  2:3772812/150‑1 (MQ=255)
                                            tATTCAGAAAGTTTGCGGGTAACCCTTAT‑AGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCct                                                                                                          <  1:618056/150‑1 (MQ=255)
                                              ttCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCctg                                                                                                         >  1:3841636/1‑150 (MQ=255)
                                               tCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctga                                                                                                        <  1:3080945/150‑1 (MQ=255)
                                                cAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGGAGAGAGGGGTTTTTTTTCcttac                                                                                                       >  2:3481671/1‑150 (MQ=255)
                                                         tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGc                                                                                             <  1:1799634/150‑1 (MQ=255)
                                                             ggTAGCCCTTATAAGCTCCATACCTATGCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGAtt                                                                                         <  2:816882/150‑1 (MQ=255)
                                                             ggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGGGAAGAGAGCGCAGGGGGGGGTTATTTTTCCTTACTGAAC‑TGGCGGtt                                                                                         >  2:2056917/1‑150 (MQ=255)
                                                              gTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTa                                                                                        <  2:324143/150‑1 (MQ=255)
                                                                 aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGt                                                                                     <  1:1726779/150‑1 (MQ=255)
                                                                  cccTTATAAGCTCCATACCTATACCAGTCAAGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGtt                                                                                    <  2:1878518/150‑1 (MQ=255)
                                                                   ccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGttt                                                                                   <  2:721185/150‑1 (MQ=255)
                                                                      tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCcg                                                                                <  1:1551802/150‑1 (MQ=255)
                                                                            cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGCGAAAGGAGCGCAGGGGGGGGTTATTTTTCCTTGCTTAAC‑TCGCGGGTTAGGTTTCGCGTAcc                                                                          >  2:133675/1‑150 (MQ=255)
                                                                              ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCg                                                                        <  1:3629003/150‑1 (MQ=255)
                                                                                 tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCg                                                                     <  1:1663971/150‑1 (MQ=255)
                                                                                  aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGt                                                                    <  1:4014889/150‑1 (MQ=255)
                                                                                  aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGt                                                                    <  1:1178073/150‑1 (MQ=255)
                                                                                     tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCATTAGAAGAGAGCGGAGAGAGGGGGTATCTTGCCTTGCTGAAC‑TCGCGGTTAAGTTTTCCCGTACCCCGCGtttt                                                                 >  2:1826245/1‑149 (MQ=255)
                                                                                        aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGtt                                                              <  1:1815690/150‑1 (MQ=255)
                                                                                         ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTa                                                             <  1:2140107/150‑1 (MQ=255)
                                                                                          cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCATGAGAAGGGAGGGGGGGGGGGGGGTATCTTTCCTGGCTGAAA‑TGGCGGTTTAGTTTTCCCGGACCCGGCGgtttggttt                                                            >  2:2913729/1‑144 (MQ=255)
                                                                                           aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCATGAGAAGGGAGAGCAGAGAGGGGTTATCTTGCCTGACTGGAC‑TGGCGAGTAAGTTTCCGCGGACCCGGCGGGTAGTTAtt                                                           >  2:1436801/1‑150 (MQ=255)
                                                                                            gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                                          <  1:3317191/150‑1 (MQ=255)
                                                                                                  aGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatg                                                    <  2:223429/150‑1 (MQ=255)
                                                                                                  aGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatg                                                    <  2:1246169/150‑1 (MQ=255)
                                                                                                   gTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatga                                                   <  1:195302/150‑1 (MQ=255)
                                                                                                    tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCCGAGAGGGGTTATTTTTCCTGGCTGAAC‑CGGCGGTTAAAGTTTCGCGGACCCCGCGGTTTGTTTTTTTGgatggt                                                  >  2:2187591/1‑148 (MQ=255)
                                                                                                     cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatg                                                 <  1:984903/150‑1 (MQ=255)
                                                                                                      aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatga                                                <  1:2360414/150‑1 (MQ=255)
                                                                                                      aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatga                                                <  1:688201/150‑1 (MQ=255)
                                                                                                          cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATAtt                                            <  1:3742312/150‑1 (MQ=255)
                                                                                                          cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCAGAGAGGGGGTATCTTTCCTGACTGAAC‑TGGCGGTTTAATTTTCGCGTACCCCGCGGTTTGTTATTTTGGGTGGTGATAtt                                            >  2:2654104/1‑150 (MQ=255)
                                                                                                            ggATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGTGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGa                                          <  1:3896554/150‑1 (MQ=255)
                                                                                                            ggATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGa                                          <  1:1262980/150‑1 (MQ=255)
                                                                                                             gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGaa                                         <  1:1470528/150‑1 (MQ=255)
                                                                                                              aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                                        <  2:3977744/150‑1 (MQ=255)
                                                                                                               tGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCTGAGAAGGGAGGGCCGGGGGGGGTTATCTTTCCTGGCTGTAC‑TGGCGGTTAAGTTTTCGCGTACCCCGCGGTTTGTTATTTCGGGTGATGGTAttttagc                                       >  2:2722795/1‑145 (MQ=255)
                                                                                                                    tGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGGGAAGGGAGGGCAGAGAGGGGGTTTTTTTTCTGACTTAACTTGGGGTTTAAGTTTCCG‑GTTCCCGGGGGTTTGTTTTTGTGGGTGATGGTAATTAATCTCGGc                                  >  2:3806883/1‑150 (MQ=255)
                                                                                                                      tCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTg                                <  2:2123749/150‑1 (MQ=255)
                                                                                                                       cgcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGa                               <  1:2887133/150‑1 (MQ=255)
                                                                                                                        gcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAt                              <  1:1466453/150‑1 (MQ=255)
                                                                                                                         cgGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGGGAAGGGAGCGCAGAGAGGGGGTATCTTTCCTGACTTGAA‑TGGCGATTAAGTTTTCCCGTACCCCGCGGTTTGTTATTGCGGGTGATGATAATGAAGCTCgggcggtt                             >  2:420121/1‑144 (MQ=255)
                                                                                                                          ggATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTg                            <  2:1175033/150‑1 (MQ=255)
                                                                                                                          ggATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTg                            <  1:3349361/150‑1 (MQ=255)
                                                                                                                          ggATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTg                            <  2:3384798/150‑1 (MQ=255)
                                                                                                                           gATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGGGCCGAGAGGGGGTATTTTTCCTGACTGAAC‑TTGCGATTAAATTTTCGCGTACCCCGCGGTTTGTTTTTTCGGGTGATGATATTTTAACTCCGCTGGtttg                           >  2:1961115/1‑148 (MQ=255)
                                                                                                                            aTCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGAGAGGGGGGGGGGGGGTTATTTTTCCCTACTTAAC‑TTGCGGTTAAAATTTCCCGTTCCCCGCGGTTTATTATTTCGGGTGATGAGATTTAAGCTCGGCttgtttgt                          >  2:1562910/1‑143 (MQ=255)
                                                                                                                               tcGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCa                       <  2:3663193/150‑1 (MQ=255)
                                                                                                                                 gTTTGCGGCGGTCATTTTTTCTTTTCCTGCCATAAGAAGTGAGCGCGGAGAGCGATTATCTTGCCTGACTGAAC‑TGTCAATTAAGTTTCCGCTTTCCCGCCGTTTAGTTATTGCGGATGATAAAATTGAAGCACATTTGAGTGGTTTAtt                     <  2:887744/150‑1 (MQ=255)
                                                                                                                                     gcggcgGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGGGGGGTTTTCTTTCCTTACTTAAC‑TGGCGATTAAGTTTCCCCGTACCCGGCGTTTTGTTATTGCGGATGATGATATTTAAGCTCGGCTTATTGGTTCATTTTCg                 >  2:520105/1‑150 (MQ=255)
                                                                                                                                     gcggcgGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCg                 <  2:307785/150‑1 (MQ=255)
                                                                                                                                        gcggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCa              <  2:656452/150‑1 (MQ=255)
                                                                                                                                        gcggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCa              >  2:92445/1‑150 (MQ=255)
                                                                                                                                         cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt             <  1:3687781/150‑1 (MQ=255)
                                                                                                                                           gTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCa           >  2:3555884/1‑150 (MQ=255)
                                                                                                                                             cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAcc         >  1:1211198/1‑150 (MQ=255)
                                                                                                                                               ttttttCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGc       <  2:487243/150‑1 (MQ=255)
                                                                                                                                                tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCt      >  1:2734976/1‑150 (MQ=255)
                                                                                                                                                tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCt      >  2:2383996/1‑150 (MQ=255)
                                                                                                                                                  tttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTgg    >  1:2998305/1‑150 (MQ=255)
                                                                                                                                                  tttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTgg    >  1:922194/1‑150 (MQ=255)
                                                                                                                                                   ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGGTTGGTTCATTGTCGCCATCACCGCTGGa   >  2:548065/1‑150 (MQ=255)
                                                                                                                                                   ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGa   <  1:2236368/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAC‑TGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAC  >  AP012306/3938738‑3939035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑