Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306341,3770TG41.6% 168.4 / 44.2 137G60G (GGT→GGG) ribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
Reads supporting (aligned to +/- strand):  ref base T (14/66);  new base G (57/0);  total (71/66)
Fisher's exact test for biased strand distribution p-value = 1.48e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 205 total aligned reads displayed.

AAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCG  >  AP012306/341230‑341526
                                                                                                                                                    |                                                                                                                                                     
   gCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGaaaa                                                                                                                                                  <  2:2588857/150‑1 (MQ=255)
    cTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGGGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCCACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGacaaa                                                                                                                                                 >  2:2643644/1‑150 (MQ=255)
     tGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGgaaaaa                                                                                                                                                >  2:2543051/1‑149 (MQ=255)
      ggCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACTAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAgg                                                                                                                                               >  2:110685/1‑149 (MQ=255)
      ggCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGc                                                                                                                                               <  1:2471390/150‑1 (MQ=255)
      ggCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGgaaaaac                                                                                                                                               >  1:3733709/1‑148 (MQ=255)
       gcgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCTGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGcc                                                                                                                                              <  2:1781396/150‑1 (MQ=255)
        cgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGgaaaaaacc                                                                                                                                             >  1:1117807/1‑146 (MQ=255)
          cAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCaaa                                                                                                                                           <  1:4361556/150‑1 (MQ=255)
           aaCGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAg                                                                                                                                          >  1:1414843/1‑150 (MQ=255)
            aCGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAgg                                                                                                                                         <  1:892438/150‑1 (MQ=255)
            aCGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAgg                                                                                                                                         <  1:3968972/150‑1 (MQ=255)
             cGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGt                                                                                                                                        <  1:3437601/150‑1 (MQ=255)
              gAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTg                                                                                                                                       <  2:2889362/150‑1 (MQ=255)
               aGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGc                                                                                                                                      <  1:1502289/150‑1 (MQ=255)
                   cGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGAcc                                                                                                                                  <  2:3540725/150‑1 (MQ=255)
                     ttttACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGc                                                                                                                                <  1:1876793/150‑1 (MQ=255)
                     ttttACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGCGACCGc                                                                                                                                >  1:4564263/1‑150 (MQ=255)
                     ttttACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAGGGGCGACCGc                                                                                                                                >  1:134217/1‑150 (MQ=255)
                      tttACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCGAAAGGGCGGCCggc                                                                                                                               >  1:4247501/1‑148 (MQ=255)
                       ttACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCt                                                                                                                              <  1:2172833/150‑1 (MQ=255)
                       ttACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGTGCGGCccccc                                                                                                                              >  1:3368458/1‑149 (MQ=255)
                        tACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTa                                                                                                                             <  1:250543/150‑1 (MQ=255)
                        tACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGAGCGACCGCCTa                                                                                                                             >  1:4284475/1‑150 (MQ=255)
                           cACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAAGCAAAAGAGCGGCCGCCTATGt                                                                                                                          >  1:3894316/1‑150 (MQ=255)
                            aCGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTGGCGTATGGG‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTc                                                                                                                         <  1:957484/150‑1 (MQ=255)
                             cGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGAGCGACcccccagatca                                                                                                                        >  2:720149/1‑143 (MQ=255)
                             cGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGAGGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTca                                                                                                                        <  1:293582/150‑1 (MQ=255)
                              gCATACCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTcac                                                                                                                       <  1:1550218/150‑1 (MQ=255)
                                accccACCCCGAATGTGGGGTGCGTCATTGTCAAAGATGGGGAAATTGTCGGTAAAGGTTACCACCAACGTGCGGGTAAACCACATGCGGAAGTACCCGCTTTGGGTATGGC‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCacac                                                                                                                     <  2:3105388/148‑1 (MQ=255)
                                 tCCCAACCCGAATGTGGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTCCCACAAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCAAAAGGTGCGACCGCCTATGTCACACt                                                                                                                    <  2:2484326/150‑1 (MQ=255)
                                 tCCCAACCCGAATGTCGGGTGCGTCATTGTAAAAGATGGCGAAATTGTCGGTAAGGTTTACCACAAACGTGGGGGTGAACCACATGCCGAAGAACCCGCGTTGCGTAGGGG‑GGGTAAAAAAGCAAAAGGTGCGTCCGCCTATGTCACACt                                                                                                                    <  2:4285108/150‑1 (MQ=255)
                                  cccAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGG‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTc                                                                                                                   <  1:4378605/150‑1 (MQ=255)
                                     aaCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAAACAAAAGGGGGACCCCCCATGTTACACTCGaa                                                                                                                >  2:3435984/1‑150 (MQ=255)
                                     aaCCCGAATGGCGGGTGCGTCATTGACAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGaa                                                                                                                <  1:2651291/150‑1 (MQ=255)
                                          gAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGGAAAAACCAAAAAGGGGACCGCCCATTTTACACTCGCAccccg                                                                                                           >  2:1848946/1‑148 (MQ=255)
                                           aaTGTCGGGGGCGTCATTGCAAAAAATGGCAAATTTGCTGGTAAAGGTTACCACAATCGTGCGGGAGACCCACATGCCTAACTACACTGCTGGGGTATGGG‑GGGTAAAAAACCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGt                                                                                                          <  2:597724/150‑1 (MQ=255)
                                             tgtgGGGTGCGTCATTGTAAAAGATGGCAAAATTGTCGGTGAAGTTTCCCCCCACCGTGCGGGTGACCCACATGCCGAAGTCCCCGCGTTGCGTAGGGC‑GGGTAAAAAACCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAg                                                                                                        <  2:772400/150‑1 (MQ=255)
                                             tGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTCCCACCAACGTGGGGGTGACCCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAg                                                                                                        <  2:854797/150‑1 (MQ=255)
                                               tCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGcc                                                                                                      <  1:3175557/150‑1 (MQ=255)
                                                cGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGCCAAAAGCGCGACCGCCCATATCACACTCGAAACCCTTAGCca                                                                                                     >  2:366392/1‑150 (MQ=255)
                                                   gTGCGTCTTGTTAAAAGATGGGAAAATTGTCGGGGAGGGTTACCGCCAGCGTGCGGGTGACCCACATGCCGAAGTACCCGCGTTGCGTTTGCC‑GGGTGAAAAAGCAAAAGGGGCGTCCGCCTATGTCACACTCGAACCCTGTAGCcatca                                                                                                  <  2:3552444/150‑1 (MQ=255)
                                                    tGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAGGAGGGGCCGCCCATTTTCAACCCCAACCCCGTAGGcatcaa                                                                                                 >  2:199068/1‑149 (MQ=255)
                                                      cGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAGGGGCGACCGCCTATGTCACACTCGAACCCCGTAACCCTCATgg                                                                                               >  1:2312293/1‑150 (MQ=255)
                                                      cGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCCGTAGCCATCATgg                                                                                               >  1:2286507/1‑150 (MQ=255)
                                                      cGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCGGCCGCCTCTGTCACACCCGAACCCCTTAGCCACCAAgg                                                                                               >  2:507163/1‑150 (MQ=255)
                                                       gtatttGTAAAAGAGGGCAAAATGGTGGGGAAAGGTTACCCCCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGG‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGt                                                                                              <  2:2296802/146‑1 (MQ=255)
                                                       gTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGt                                                                                              <  1:4088569/150‑1 (MQ=255)
                                                       gTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAAGGGCGACCGCCCATGGCACACTCGCACCCCGTAGCCATCATGGt                                                                                              >  2:1523719/1‑150 (MQ=255)
                                                        tCATTGTCAAAGATGGCGAAATTGTGGGTGAAGGTTACCACCACCGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTc                                                                                             <  2:222974/150‑1 (MQ=255)
                                                        tCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAAGGCGACCGCCCATGTCCCACACGAACCCCGTAGCcatcaacggc                                                                                             >  2:1270565/1‑145 (MQ=255)
                                                        tCATTGTCAAAGATGGCAAAATTGTGGGTGAGGTTTCCCCCCAACGTGCGGGTGACCCACATGCCGAAGTACACGCGTTGCTTAGGGG‑GGGGAAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTc                                                                                             <  2:3323866/150‑1 (MQ=255)
                                                        tCATTGTAAAAAATGGCAAAATTGTCGGGGAAGTTTCCCACCAACGTGCGGGTGAACCACAGGCCGAAGTACCCGCTTTCCTTATGGC‑GGGTAAAAAAGCAAAAGGTCCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTc                                                                                             <  2:246377/150‑1 (MQ=255)
                                                           ttGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGTGGGACCCCCTATATTACACTCGAACCCTGTAGCCATCATGGTCGTa                                                                                          >  1:5366/1‑150 (MQ=255)
                                                             gTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCGACCGCCCAAGTCACACTCGAACCCCGTAGCCATCATGGTCGTACg                                                                                        >  2:2636018/1‑150 (MQ=255)
                                                                  aGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGCGACCGCCCATGTCACACTCGAACCCCGTAGCCCTCATGGTCGTACGCcccc                                                                                   >  2:3900426/1‑150 (MQ=255)
                                                                   gATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCg                                                                                  <  1:357202/150‑1 (MQ=255)
                                                                     tGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAAGCAAAAGG‑GCGACCGCCCATATCACACTCGCACCCCGTAGCCACCACGGTCGTAAGCCACCCTg                                                                                >  2:3630394/1‑150 (MQ=255)
                                                                     tGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCGACCCCCCATGTCACACCCCCAACCCGTAGCCCACAAGGGCGGACGCccccccg                                                                                >  2:3487058/1‑147 (MQ=255)
                                                                          aaaTTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAAACAAAAGGGCGACCGCCCCAGTCAAACACCAACCCCTTAGGCAACATGGGCGTACGCCCCCCCGCtgtg                                                                           >  2:2622409/1‑150 (MQ=255)
                                                                           aaTTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAAGAGCGACCGCCCCTGTCACACCCCAACCCCGTAGCCCTCATGGTCGTACGCCCCCGCGCTGTGa                                                                          >  2:2165722/1‑150 (MQ=255)
                                                                           aaTTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCGACCGCCTATGTCACACCCGAACCCTGTAGCCATCATTGTCGGACGCCCCCCGGCTGTGa                                                                          >  2:694695/1‑150 (MQ=255)
                                                                               gTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCGGCCGCCTATGTCCCACTCCGAACCTGTAAGCATCCTGGGCGTACGCCCCCGGGCTGTGACGCa                                                                      >  2:453794/1‑150 (MQ=255)
                                                                                tCGGGGAAGGTTACCACCAACGGGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCAc                                                                     <  2:2843265/150‑1 (MQ=255)
                                                                                  ggTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTc                                                                   <  1:1431829/150‑1 (MQ=255)
                                                                                   gTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCa                                                                  <  1:3296945/150‑1 (MQ=255)
                                                                                   gTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGCGCTGTGACGCACTCa                                                                  >  1:1754738/1‑150 (MQ=255)
                                                                                     gAAGTTTCCCCCCACCGTGCGGGTGCCCCACATCCCGAAGTCCACGCTTTCCTTATGGC‑GGGTGAAAACCCCAAAGGTGCCACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATc                                                                <  2:3090814/150‑1 (MQ=255)
                                                                                     gAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGGGCGACCGCCCATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATc                                                                >  1:651486/1‑150 (MQ=255)
                                                                                         ttcaccaccaccGTGCGGGTCACCCACATCCCAAAGTACCCGCGTTGCGTTTGGC‑GGTTAAAAACCCCAAAGTTCCGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATcgccg                                                            <  2:2799317/147‑1 (MQ=255)
                                                                                            accaccAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTg                                                         <  1:4158810/150‑1 (MQ=255)
                                                                                             ccaccaACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTgg                                                        <  1:2402092/150‑1 (MQ=255)
                                                                                             ccaccaACGTGCGGGTAAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTgg                                                        <  1:3069244/150‑1 (MQ=255)
                                                                                              cacaaACGTGCGGGTGAACCACAGGCCGAAGTACACGCGTTGGGTATGGC‑GGGTAAAAAAGCCAAAGGTCCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGc                                                       <  1:2907601/150‑1 (MQ=255)
                                                                                                     gTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGCGACCGCCTATGTCACACTCCAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAgcgc                                                >  2:4253566/1‑150 (MQ=255)
                                                                                                          ggTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTg                                           <  1:1225502/150‑1 (MQ=255)
                                                                                                           gTGAACCACATGCCGAAGTACCCGCGTTGCGTATGGG‑GGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTgg                                          <  2:3784755/150‑1 (MQ=255)
                                                                                                            tGAACCACATGCCGAAGTACCCGCGTGGCGTATGGC‑GGGTAAAAAAGCAAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGt                                         <  1:1921419/150‑1 (MQ=255)
                                                                                                              aacaaaaTGCCAAAGTACACGCGTGGCGTAGGGC‑GGGTAAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTg                                       <  1:3409073/144‑1 (MQ=255)
                                                                                                                ccACATGCCGAAGTACACGCGTGGCGTATGGG‑GGGTAAAAAAGCAAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGcc                                     <  2:4361289/150‑1 (MQ=255)
                                                                                                                 cacaTGCCGAAGTACCCGGGTTGCGTAGGGC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCt                                    <  2:3688643/150‑1 (MQ=255)
                                                                                                                  acaGGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTc                                   <  2:2519713/150‑1 (MQ=255)
                                                                                                                   caTGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCGACCGCCCATGTCACACTCGAACCCTGTAGCCATCATGGTCGGACGCCCCCGTGCTGTGACGCACTCATCGCCGCCGGCGTAGCGCGCGTGGTTGCCTCg                                  >  2:1208476/1‑150 (MQ=255)
                                                                                                                     ggCCGAAGTCCACGGGTTGCGTAGGGC‑GGGTGAAAAAGCAAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGAt                                <  2:1722021/149‑1 (MQ=255)
                                                                                                                      gCCGAAGTACCCGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATg                               <  1:3209472/150‑1 (MQ=255)
                                                                                                                        cGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAAGGGGGGCCGCCCATGTCACACTCGAACCCCGTAGCCATCATGGTCGTACGCCACCCCGCTGTGACGCACTCATCCCCCCTGGCGTAGCGCGCGTGGGTGCCTCGATGCa                             >  2:3007702/1‑150 (MQ=255)
                                                                                                                          aaGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCCCGGTTGCCTCGCTGCCAg                           >  1:3795700/1‑150 (MQ=255)
                                                                                                                           aGTACGCGCGTTGGGTAGGGC‑GGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGa                          <  1:330300/150‑1 (MQ=255)
                                                                                                                              acacGCGTTGCGTATGGG‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATcc                       <  1:559472/150‑1 (MQ=255)
                                                                                                                               cacGCGTTGCGTAGGGG‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCt                      <  2:485943/150‑1 (MQ=255)
                                                                                                                                ccgcgTTGCTTATGGG‑GGGTGAAAAACCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTa                     <  2:3994582/149‑1 (MQ=255)
                                                                                                                                 cgcgTTGCGTAGGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTaa                    <  1:1239749/150‑1 (MQ=255)
                                                                                                                                  gcgTTGCGTATGGC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAAc                   <  1:2464879/150‑1 (MQ=255)
                                                                                                                                         gTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCCCAgg            >  2:1330544/1‑150 (MQ=255)
                                                                                                                                           aTGGG‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTc          <  1:2541520/150‑1 (MQ=255)
                                                                                                                                                 gggTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTgg     >  1:2342046/1‑150 (MQ=255)
                                                                                                                                                  ggTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTggg    >  2:789740/1‑150 (MQ=255)
                                                                                                                                                   gTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGc   >  2:2573507/1‑150 (MQ=255)
                                                                                                                                                    tGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCg  >  2:4211029/1‑150 (MQ=255)
                                                                                                                                                    tGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCg  >  2:3071516/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCG  >  AP012306/341230‑341526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑