Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306935,0540GT23.8% 303.7 / 44.3 105intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
Reads supporting (aligned to +/- strand):  ref base G (15/65);  new base T (25/0);  total (40/65)
Fisher's exact test for biased strand distribution p-value = 4.13e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 172 total aligned reads displayed.

GGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGC‑GATTTTGACGCCACCAACA  >  AP012306/934905‑935203
                                                                                                                                                       |                                                                                                                                                          
ggCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Gtttg                                                                                                                                                            >  2:831898/1‑150 (MQ=255)
 gCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCTCTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Ttttgt                                                                                                                                                           <  1:1664702/150‑1 (MQ=255)
    gTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Gtttttttg                                                                                                                                                        >  1:2427540/1‑150 (MQ=255)
    gTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Ctttttttt                                                                                                                                                        >  1:3256794/1‑149 (MQ=255)
     tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Gttttttttg                                                                                                                                                       >  1:2732893/1‑148 (MQ=255)
       ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTGTTTggt                                                                                                                                                      >  2:1392088/1‑149 (MQ=255)
       ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGCt                                                                                                                                                     >  1:2262506/1‑150 (MQ=255)
        tGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCtt                                                                                                                                                    >  1:2381687/1‑150 (MQ=255)
        tGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCtt                                                                                                                                                    >  1:4032150/1‑150 (MQ=255)
         gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGGTTc                                                                                                                                                   >  2:385221/1‑150 (MQ=255)
          ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTca                                                                                                                                                  <  2:3122274/150‑1 (MQ=255)
            aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTcagc                                                                                                                                                >  2:4125083/1‑150 (MQ=255)
            aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTctgc                                                                                                                                                >  1:2383/1‑150 (MQ=255)
            aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGA‑GG‑GTTTGTTTGGCTTcagc                                                                                                                                                >  2:44624/1‑150 (MQ=255)
              tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTctgccg                                                                                                                                              >  1:69835/1‑148 (MQ=255)
              tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTcagcag                                                                                                                                              <  2:1526672/150‑1 (MQ=255)
                tAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGGTTCAGCAG‑Cg                                                                                                                                           >  1:739527/1‑150 (MQ=255)
                tAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCggcag‑ca                                                                                                                                           >  2:1779554/1‑149 (MQ=255)
                tAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑Cg                                                                                                                                           <  1:196308/150‑1 (MQ=255)
                 aGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGG‑GTTTGTTTGG‑GTCAGCAA‑Cgg                                                                                                                                          >  2:2103122/1‑150 (MQ=255)
                 aGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTCTGCAG‑Cgg                                                                                                                                          >  2:1456448/1‑150 (MQ=255)
                  gtgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGGTTTAGCAG‑Cggg                                                                                                                                         >  2:2418189/1‑150 (MQ=255)
                   tgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTCAGCAG‑Cgggg                                                                                                                                        >  1:3668483/1‑149 (MQ=255)
                   tgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGGTTCAGCAG‑Cgggc                                                                                                                                        >  2:4067209/1‑149 (MQ=255)
                   tgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGCTTCAGCAG‑Cgggg                                                                                                                                        >  2:4435069/1‑149 (MQ=255)
                   tgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCTTCAA‑CGGGt                                                                                                                                        >  1:1270466/1‑150 (MQ=255)
                    gtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTCCGCAG‑Cggggt                                                                                                                                       >  1:2247542/1‑148 (MQ=255)
                     tttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGc                                                                                                                                                      >  1:2634225/1‑135 (MQ=255)
                     tttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGc                                                                                                                                                      <  2:2634225/135‑1 (MQ=255)
                      ttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTCAGCAG‑CGGGGTgc                                                                                                                                     >  1:1802686/1‑150 (MQ=255)
                       tAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTTAGCCG‑Cggggttcg                                                                                                                                    >  1:308261/1‑145 (MQ=255)
                       tAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTgcg                                                                                                                                    <  1:158793/150‑1 (MQ=255)
                        aGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGG‑TTTTTTTTGGTTTC‑GCAG‑CGGGGTgggc                                                                                                                                   >  2:1196881/1‑150 (MQ=255)
                          tttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGTTTCCGCAA‑CGGGGTgggcgc                                                                                                                                 >  2:157829/1‑148 (MQ=255)
                             gCTGGGTAAAGCACCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTTGTTCCGCCG‑CGGGGTGCGCGcccc                                                                                                                              >  2:4586475/1‑149 (MQ=255)
                              cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGCTTCAGCAG‑CGGGGTGGGCTcccct                                                                                                                             >  2:2745007/1‑148 (MQ=255)
                              cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTGGCTTCAGCAG‑CGGGGTGGGCTcccct                                                                                                                             >  2:1915869/1‑148 (MQ=255)
                               tGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCAGCAGCCGGGTTGGCCTCCttt                                                                                                                             >  1:2029099/1‑150 (MQ=255)
                                 ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAAt                                                                                                                          <  1:2369576/150‑1 (MQ=255)
                                  gTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAAtg                                                                                                                         <  1:2633665/150‑1 (MQ=255)
                                  gTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAAtg                                                                                                                         <  1:1992461/150‑1 (MQ=255)
                                  gTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAAtg                                                                                                                         <  1:494718/150‑1 (MQ=255)
                                   tAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTCCGCAG‑CGGGGTGCGCTCCCTTAAttt                                                                                                                        >  1:2977381/1‑148 (MQ=255)
                                    aaaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑CTTTTTTTTGTTTCTGCAA‑CGGGTTTGGATCCCCTTAtgtt                                                                                                                       >  1:2040172/1‑149 (MQ=255)
                                      aGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGcc                                                                                                                     <  1:3589753/150‑1 (MQ=255)
                                       gcgcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTGGCCTGCGGC‑GG‑TTTTTTTTTGCTTCAGCAG‑CGGGGTGCGCTCCCCTTATTTGccc                                                                                                                    >  1:4306872/1‑149 (MQ=255)
                                          ccGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGG‑TTTTTTTTGGTTTC‑GCCG‑CGGGGTTGGCCCCCCTTATGTGcccccc                                                                                                                 >  2:3181307/1‑146 (MQ=255)
                                          ccGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGc                                                                                                                 <  1:3793878/150‑1 (MQ=255)
                                           cGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTCAGCAG‑CGGGGTGCGCTCCCCTAATGTGCCTCGcc                                                                                                                >  1:2523334/1‑150 (MQ=255)
                                           cGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGcc                                                                                                                <  1:2613611/150‑1 (MQ=255)
                                            gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCAGCAG‑CGGGGTGCGCTCCCCTTATTTGCCTCGccc                                                                                                               >  1:1235831/1‑149 (MQ=255)
                                            gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATTTGCCTCGccc                                                                                                               >  1:3962819/1‑149 (MQ=255)
                                             tCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCAGCAG‑CGGGGTGCGCTCCCTTTATGTGCCTCGCCat                                                                                                              >  1:4154772/1‑150 (MQ=255)
                                               aGCGGCGATGCAAAAACGGTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCatat                                                                                                            <  1:4273102/150‑1 (MQ=255)
                                                gcggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGCTTCAGCAG‑CGGGGTGCGCTCCCCTTATGTGCCTCGccccata                                                                                                           >  1:4460134/1‑145 (MQ=255)
                                                     gATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTg                                                                                                    <  2:1548276/150‑1 (MQ=255)
                                                      aTGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGa                                                                                                   <  1:1052904/150‑1 (MQ=255)
                                                        gCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAAt                                                                                                 <  1:2500398/150‑1 (MQ=255)
                                                          aaaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCAGCAG‑CGGGGTGCGCTCCCCTTATTTGCCCCCCCCCAT‑AAA‑ATTAATgg                                                                                               >  1:1170583/1‑150 (MQ=255)
                                                           aaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGCTTCAGCAG‑CGGGGTGCGCTCCCTTTATGTGCCTCCCCCTAT‑AAA‑ATGAATGGt                                                                                              >  1:3859920/1‑150 (MQ=255)
                                                           aaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGt                                                                                              <  1:2297748/150‑1 (MQ=255)
                                                           aaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGGTTTTGCAG‑CGGGGTTGGCTCCCCTTATTTGGCCCCCCCTCT‑AAA‑ATTAATGGt                                                                                              >  2:637498/1‑150 (MQ=255)
                                                            aaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTg                                                                                             <  2:728937/150‑1 (MQ=255)
                                                             aaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCAGCAG‑CGGGGTGCGCTCCCCTTATTTGCCTCGCCCCAT‑TAA‑TTGAATGGGGc                                                                                            >  2:4120684/1‑150 (MQ=255)
                                                                gCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGGTTCTGCAG‑CGGGGTGCGCCCCCCTTATGTGCCTCCCCCAAT‑AAA‑ATTTATTGTGCAgg                                                                                         >  2:1744216/1‑150 (MQ=255)
                                                                  tttGCCGAGGTCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGa                                                                                       <  1:1452076/150‑1 (MQ=255)
                                                                         aGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTGTTTTGCTTTCGCAG‑CGGGGTTCGCCCCCCTTATGTGCCTCGCCCTCT‑TAA‑ATGAATGGTGCAGGGAGCGGGCa                                                                                >  2:2726915/1‑150 (MQ=255)
                                                                           gCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑‑TTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCCTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAggg                                                                             >  1:3271390/1‑150 (MQ=255)
                                                                            ccGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAggg                                                                             <  1:1235756/150‑1 (MQ=255)
                                                                                    cACTATCATTCGGCTCCGCTGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCa                                                                     <  1:425808/150‑1 (MQ=255)
                                                                                    cACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGCTTCAGCAG‑CGGGGTGCGCTCCCCTAATGTGCCTCCCCATAT‑AAA‑TTTAATGGGGCAGGGAGCGCGCCgggggggggcc                                                                     >  2:2442717/1‑147 (MQ=255)
                                                                                    cACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCa                                                                     <  1:302099/150‑1 (MQ=255)
                                                                                       tATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATc                                                                  <  2:4391527/150‑1 (MQ=255)
                                                                                        aTCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATcg                                                                 <  1:2168657/150‑1 (MQ=255)
                                                                                         tCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGGTTCCGCAG‑CGGGGTGCGCTCCCTTTATGTGCCCCGCCCTAT‑AAA‑TTTAATGGTGGAGGGGGCGCGCCGGGGGGGGcccaacac                                                                >  2:3114196/1‑144 (MQ=255)
                                                                                          cATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGCTTCAGCAG‑CGGGGTGGGCCCCCCTTATGTGCCTCCCCCTATAAAA‑TTTATTGGGGAGGGGGGGCCGCGGGGGGGGGCCaacccc                                                                >  2:3686634/1‑146 (MQ=255)
                                                                                                gCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGCTTCAGCAG‑CGGGGTGCGCTCCCCTAATGTGCCTCGCCCTAT‑AAA‑TTTAATGGGGCAGGGGGGGCGCCGGGGGGGGCCCAACgcccccccc                                                         >  2:1651749/1‑150 (MQ=255)
                                                                                                 cTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGCTTTCGCAG‑CGGGGTGGGCCCCCCTTATGGGGCCCGGCCTAT‑AAATTTGAATGGGGCAGGG‑GGGCGCCGGGGGGGGGCCAACCCCCCCGccc                                                        >  2:422099/1‑150 (MQ=255)
                                                                                                    cgccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCct                                                     <  1:1839472/150‑1 (MQ=255)
                                                                                                     accgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCAGCAG‑CGGGGTGCGCCCCCCTTATGTGGCCCCCCCTAT‑AAA‑ATTAATGGGGGAGGGGGGGCGCCGGGGGGGGCCAAACcccccccccccccc                                                    >  2:81438/2‑148 (MQ=255)
                                                                                                           aCGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGCTTTAGCAG‑CGGGGTGGGCTCCCCTTATGTGCCCCGCCCTAT‑AAA‑TTTAAAGGGGCAGGGGGCGCGCCGGGGGGGGCCCAACGCCCCCCCCCCCCCCTGTcc                                              >  2:1472529/1‑150 (MQ=255)
                                                                                                                  cAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCtt                                       <  2:643062/150‑1 (MQ=255)
                                                                                                                  cAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCtt                                       <  2:3771218/150‑1 (MQ=255)
                                                                                                                   aGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTCAGCAG‑CGGGGTGGGCCCCCCCTATTTGGCCCCCCCTAT‑AAA‑TTTAATGGGGGCGGGGGGGCGCAGGGGGGGGGCACACGCCCCCCCCCCCCCCCGTCCCCGCCCTc                                      >  2:3008742/1‑150 (MQ=255)
                                                                                                                    ggAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑g                                    <  1:1065895/150‑1 (MQ=255)
                                                                                                                      aaaGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑ggg                                  <  1:3384375/150‑1 (MQ=255)
                                                                                                                          cTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGGTTCCGCAG‑CGGGGTGCGCTCCCCTTATTTGGCCCCCCCTAT‑AAA‑TTTAATGGTGGAGGGGGGGCGCCGGGGGGGGGCCATCGCCCCCGCCCCCCTCTTTGCCGGGCTTCTGGGGaa                               >  2:2755879/1‑150 (MQ=255)
                                                                                                                                  cTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCCGCAG‑CGGGGTGCGCCCCCCTTATTTGGCCCGCCCTTT‑AAA‑TTTTATGGGGGAGGGGGGGCGCCGGGGGGGGCCCATTCCCCCCCCCCCCCCCTGTCCCCGCCCTC‑TGGGGACGCCTcccc                      >  2:961143/1‑147 (MQ=255)
                                                                                                                                  cTTAGCCTGCGGC‑GG‑TTTTTTTTTGCTTCAGCCG‑CGGGGTGCGCCCCCCTTATGTGGCTCCCCCTAT‑AAA‑TTTAATGGTGGAGGGGGCGCGCCGGGGGGGGCCCATCGCCCCCCCCCCCCGCTGTCCCCGCCCTC‑GGGGGAAGCCTCAGc                      >  2:772019/1‑150 (MQ=255)
                                                                                                                                       ccTGGGGC‑GG‑TTTTGTTTGGCTTCGGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGC‑GAttt                <  2:3357899/150‑1 (MQ=255)
                                                                                                                                       ccTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGC‑GAttt                <  1:1932178/150‑1 (MQ=255)
                                                                                                                                           cggc‑gg‑TTTTGTTTGGCTTCAGCAG‑GGGGGGGCGCTCCCCTAATGTGGCTCCACCTGTAAAA‑TTGAGAGGGGC‑GGGAGCGCGCAGGGGGCGGCCGAACGCCGCCCCCCCCCGCGTTCCCCGCCCTC‑GGGGGACACCTCCGCGGAtttttc             >  2:1256368/1‑148 (MQ=255)
                                                                                                                                            ggc‑gg‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGGACGCTTCAGC‑GATTTTGACg           >  1:3168636/1‑150 (MQ=255)
                                                                                                                                              c‑gg‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGC‑GATTTTGACGcc         <  2:929184/150‑1 (MQ=255)
                                                                                                                                                 g‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGC‑GATTTTGACGccac       <  1:1858495/150‑1 (MQ=255)
                                                                                                                                                   ttttGTTTGGCTTCAGCAG‑CGGGTTGGGCTCCCTTAAGGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGC‑GATTTTGACGccacc      <  2:1329132/150‑1 (MQ=255)
                                                                                                                                                   ttttGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGC‑GATTTTGACGccacc      >  2:3542871/1‑150 (MQ=255)
                                                                                                                                                      tgtttgGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGC‑GATTTTGACGCCACCAac   <  2:3494409/150‑1 (MQ=255)
                                                                                                                                                      tgtttgGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGC‑GATTTTGACGCCACCAac   >  2:1443598/1‑150 (MQ=255)
                                                                                                                                                       gtttgGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGC‑GATTTTGACGCCACCAaca  >  1:3676842/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                          
GGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTCAGCAG‑CGGGTTGCGCTCCCTTAATGTGCCTCGCCATAT‑AAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTC‑GGGGAACGCTTCAGC‑GATTTTGACGCCACCAACA  >  AP012306/934905‑935203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑