Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,128,4120TG24.0% 754.6 / 50.2 300V29G (GTG→GGG) yciOconserved protein
Reads supporting (aligned to +/- strand):  ref base T (51/177);  new base G (72/0);  total (123/177)
Fisher's exact test for biased strand distribution p-value = 3.77e-36
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 359 total aligned reads displayed.

GCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTATT  >  AP012306/1128265‑1128561
                                                                                                                                                   |                                                                                                                                                      
      gTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGttt                                                                                                                                                <  1:4114812/150‑1 (MQ=255)
       ttGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTa                                                                                                                                               >  2:3506306/1‑150 (MQ=255)
              cGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACt                                                                                                                                        <  2:3631133/150‑1 (MQ=255)
               gTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGAGTGTTTATCCAACTg                                                                                                                                       >  1:1783743/1‑150 (MQ=255)
                tCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGa                                                                                                                                      >  1:4525356/1‑150 (MQ=255)
                 cTGGCAGCTATGGGAACAGCCGCAGAACGCCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGAt                                                                                                                                     <  1:2109525/150‑1 (MQ=255)
                    gCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTTTCCAAATGAtttc                                                                                                                                  >  2:1322442/1‑148 (MQ=255)
                     cAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCg                                                                                                                                 <  2:3599091/150‑1 (MQ=255)
                           tGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATg                                                                                                                           <  2:3120922/150‑1 (MQ=255)
                           tGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATg                                                                                                                           <  1:4169413/150‑1 (MQ=255)
                            gggAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATgc                                                                                                                          <  2:2668228/150‑1 (MQ=255)
                            gggAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATgc                                                                                                                          >  1:394251/1‑150 (MQ=255)
                               aaCAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCt                                                                                                                       <  1:161668/150‑1 (MQ=255)
                                  aGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCgg                                                                                                                    <  1:3224105/150‑1 (MQ=255)
                                  aGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATTCAACTGATTTCGGCTATGCGCTCgg                                                                                                                    >  1:1926849/1‑150 (MQ=255)
                                   gCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGc                                                                                                                   >  1:1841077/1‑150 (MQ=255)
                                     cGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTg                                                                                                                 >  1:2342564/1‑150 (MQ=255)
                                       cAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTa                                                                                                               <  2:1273552/150‑1 (MQ=255)
                                       cAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTa                                                                                                               <  1:1068271/150‑1 (MQ=255)
                                         gAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTaaa                                                                                                             <  1:785562/150‑1 (MQ=255)
                                          aaCACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTaaaa                                                                                                            >  1:3601053/1‑150 (MQ=255)
                                            caccacAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAAtt                                                                                                          <  1:684346/150‑1 (MQ=255)
                                             accacAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTg                                                                                                         <  1:4131368/150‑1 (MQ=255)
                                               cacaGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTTTCCAAATGATTCCGGCTATGCGCTCGGGTGTaaaaattaa                                                                                                       >  2:4577873/1‑145 (MQ=255)
                                                 caGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGa                                                                                                     <  2:4328561/150‑1 (MQ=255)
                                                 caGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGAGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGa                                                                                                     <  1:3048629/150‑1 (MQ=255)
                                                  agagagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACGAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTATCCAACTGATTCCGGGTATTCGCTCGGGTGGAAAATTTAAGAt                                                                                                    >  2:1540519/1‑150 (MQ=255)
                                                  agagagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAt                                                                                                    >  2:3888540/1‑150 (MQ=255)
                                                   gagagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATTCGCTCGGCTGTAAAATTGAAGAT‑a                                                                                                  >  1:1399066/1‑150 (MQ=255)
                                                    agagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑aa                                                                                                 >  1:2183133/1‑150 (MQ=255)
                                                      agGGAATTATGAGCCAG‑TTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑aaaaa                                                                                              <  1:3429202/150‑1 (MQ=255)
                                                         gAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAAcc                                                                                            >  1:3329967/1‑149 (MQ=255)
                                                            ttATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGa                                                                                         >  1:1673498/1‑150 (MQ=255)
                                                             tATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAAAGCGAt                                                                                        >  1:2765640/1‑150 (MQ=255)
                                                              aTGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGCTCGTGCGTAAAGGCGGGGGGATTGTTTTTCCAACTTATTACGGCTATGCGGCCGGCTGTGAAAATGTAGAT‑TAAAACGCGATg                                                                                       >  2:1939352/1‑150 (MQ=255)
                                                              aTGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATg                                                                                       <  2:1749832/150‑1 (MQ=255)
                                                               tGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATgg                                                                                      >  1:3709473/1‑150 (MQ=255)
                                                                  gCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCCATGCGCTCGGCTGTAAAATTTAAGAT‑AAAAAAGCGATGGAgg                                                                                   >  2:2133191/1‑149 (MQ=255)
                                                                   ccAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGGAAAATTGAAGAT‑AAAAAAGCGATGGGGCg                                                                                  >  2:622278/1‑150 (MQ=255)
                                                                    cAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGTGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGt                                                                                 >  1:2218626/1‑150 (MQ=255)
                                                                        ttttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTAttt                                                                             <  2:2051202/150‑1 (MQ=255)
                                                                        ttttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTAttt                                                                             >  2:3774823/1‑150 (MQ=255)
                                                                          ttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGGGCGTATTTGt                                                                           >  2:4031422/1‑150 (MQ=255)
                                                                          ttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAAAGCGATGGAGCGGAttttt                                                                           >  2:3179100/1‑148 (MQ=255)
                                                                           tttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTc                                                                          <  2:2552025/150‑1 (MQ=255)
                                                                             tataTTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGt                                                                        <  2:84481/150‑1 (MQ=255)
                                                                             tataTTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGt                                                                        <  2:56481/150‑1 (MQ=255)
                                                                             tataTTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGt                                                                        <  1:2473557/150‑1 (MQ=255)
                                                                             tataTTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTTTCGt                                                                        >  2:2398541/1‑150 (MQ=255)
                                                                                atTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTAtt                                                                     <  2:1062772/150‑1 (MQ=255)
                                                                                atTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTAtt                                                                     <  1:2301551/150‑1 (MQ=255)
                                                                                atTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTAtt                                                                     <  2:2012491/150‑1 (MQ=255)
                                                                                atTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGGGCGTATTTTTCGTAtt                                                                     >  2:4114357/1‑150 (MQ=255)
                                                                                atTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTTTCGTAtt                                                                     >  2:1654449/1‑150 (MQ=255)
                                                                                   cATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTTTCGTATTCGc                                                                  >  1:4260095/1‑150 (MQ=255)
                                                                                    aTCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGGATTTGTCGTATTCGcc                                                                 >  2:3484091/1‑150 (MQ=255)
                                                                                    aTCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCCCGGCTGTAAAATTTAAGAT‑AAAAAAGCGATGGGGCGGATTTTTCGTATTCGcc                                                                 >  2:3367443/1‑150 (MQ=255)
                                                                                      ccTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAg                                                               <  2:2981550/150‑1 (MQ=255)
                                                                                         gATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTg                                                            <  2:1442722/150‑1 (MQ=255)
                                                                                         gATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTg                                                            <  2:2776567/150‑1 (MQ=255)
                                                                                          aTAACCCACAGCAACGTCTGATCAACCAGGCGGTGGGGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTCATTCCGGCTATGCGCTCGGCTGGAAAATTGAAGATAAAAAACGC‑CTGGGGCGGATTTTTCGTATTTAGCAGCTGc                                                           >  2:1194727/1‑150 (MQ=255)
                                                                                          aTAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGc                                                           <  1:902194/150‑1 (MQ=255)
                                                                                              cccACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTTAAGAT‑AAAAACGCGATGGAGCGTATTTTTCGTATTCGCCAGCTGCCCGa                                                       >  2:397282/1‑150 (MQ=255)
                                                                                              cccACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGa                                                       >  1:3248677/1‑150 (MQ=255)
                                                                                               ccACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGAc                                                      <  1:3758410/150‑1 (MQ=255)
                                                                                                    gCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTca                                                 <  1:2704535/150‑1 (MQ=255)
                                                                                                        cGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAAAGCGATGGAGCGGATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAAc                                             >  2:4203123/1‑150 (MQ=255)
                                                                                                           cTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACttt                                          <  2:4478518/150‑1 (MQ=255)
                                                                                                                     aGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGGTTCCGGCTTTGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGGGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGAtgt                                >  2:3728794/1‑150 (MQ=255)
                                                                                                                     aGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGAtgt                                <  2:1422399/150‑1 (MQ=255)
                                                                                                                     aGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGAtgt                                <  1:1029688/150‑1 (MQ=255)
                                                                                                                      ggCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGAtgtg                               <  1:130208/150‑1 (MQ=255)
                                                                                                                      ggCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGAtgtg                               <  2:790172/150‑1 (MQ=255)
                                                                                                                        cGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTc                             <  2:1833900/150‑1 (MQ=255)
                                                                                                                         ggTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTcg                            >  1:3567981/1‑150 (MQ=255)
                                                                                                                            ggAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGGTGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGa                         >  1:4020086/1‑150 (MQ=255)
                                                                                                                             gagaTCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGAt                        <  2:2252300/150‑1 (MQ=255)
                                                                                                                             gagaTCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGAt                        <  1:1197130/150‑1 (MQ=255)
                                                                                                                             gagaTCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGAt                        >  1:499689/1‑150 (MQ=255)
                                                                                                                               gaTCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCt                      >  1:602829/1‑150 (MQ=255)
                                                                                                                                aTCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCtt                     >  2:9211/1‑150 (MQ=255)
                                                                                                                                aTCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCCCGGCTGTAAAATTGAAGAT‑AAAAACGGGATGGGGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGTTGTTTCGCGATCtt                     >  2:2072683/1‑150 (MQ=255)
                                                                                                                                 tCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCttt                    <  1:2619632/150‑1 (MQ=255)
                                                                                                                                  cGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTc                   >  2:2887859/1‑150 (MQ=255)
                                                                                                                                    tGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTg                 >  1:1541588/1‑150 (MQ=255)
                                                                                                                                    tGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTg                 <  2:680417/150‑1 (MQ=255)
                                                                                                                                      cGTAAAGGCGGGGTGATTGTTTTTCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTTAAGAT‑AAAAACGCGGTGGGGCGTATTTGTCGTATTTCCCCGCTGCCCGACGGTCACAACTTTACCCTGATGTGTCGCGATCctttttga               >  2:1117137/1‑145 (MQ=255)
                                                                                                                                      cGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGaa               >  1:273041/1‑150 (MQ=255)
                                                                                                                                      cGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCCGATTCGCCCGCTGCCCGAAGGTCACAACTTTACCCTGATGTGTTGCGATCCTTCTTaa               >  2:4410163/1‑150 (MQ=255)
                                                                                                                                        tAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAAct             <  2:2315280/150‑1 (MQ=255)
                                                                                                                                         aaaGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAActc            <  1:383784/150‑1 (MQ=255)
                                                                                                                                          aaGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAActct           >  1:861576/1‑150 (MQ=255)
                                                                                                                                           aGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACtctc          <  1:2013829/150‑1 (MQ=255)
                                                                                                                                           aGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACtctc          <  1:2597657/150‑1 (MQ=255)
                                                                                                                                             gCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGa        <  2:4597051/150‑1 (MQ=255)
                                                                                                                                                gggTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCt     <  2:2443668/150‑1 (MQ=255)
                                                                                                                                                gggTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCt     <  1:4324450/150‑1 (MQ=255)
                                                                                                                                                  gTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTAt   >  1:955593/1‑150 (MQ=255)
                                                                                                                                                  gTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTAt   <  1:1142457/150‑1 (MQ=255)
                                                                                                                                                  gTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTCTCGCGATCTTTCTGAACTCTCGACCTAt   >  2:1619040/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
GCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGAT‑AAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTATT  >  AP012306/1128265‑1128561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑