Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,226,5570AG29.4% 358.0 / 86.2 180G55G (GGA→GGG) ydcUpredicted spermidine/putrescine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (16/111);  new base G (53/0);  total (69/111)
Fisher's exact test for biased strand distribution p-value = 1.23e-31
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 209 total aligned reads displayed.

GCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGC  >  AP012306/1226410‑1226706
                                                                                                                                                    |                                                                                                                                                     
gCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGAtt                                                                                                                                                     <  2:1894390/150‑1 (MQ=255)
 cAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGttt                                                                                                                                                    >  2:548268/1‑150 (MQ=255)
 cAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGAttt                                                                                                                                                    <  1:3200709/150‑1 (MQ=255)
   aTCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGAttttt                                                                                                                                                  >  1:5217/1‑149 (MQ=255)
   aTCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTta                                                                                                                                                  <  2:3033177/150‑1 (MQ=255)
     cACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGttttttt                                                                                                                                                >  2:3124/1‑147 (MQ=255)
       ccTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACt                                                                                                                                              <  1:3708726/150‑1 (MQ=255)
        cTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTAAtt                                                                                                                                             >  1:2605737/1‑150 (MQ=255)
          tCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTACtttt                                                                                                                                           >  1:1068883/1‑150 (MQ=255)
           cACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTTTAAttttt                                                                                                                                          >  1:3395840/1‑149 (MQ=255)
           cACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTg                                                                                                                                          <  2:3625423/150‑1 (MQ=255)
           cACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTg                                                                                                                                          <  1:2169644/150‑1 (MQ=255)
            aCGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCTGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGa                                                                                                                                         <  2:2051393/150‑1 (MQ=255)
            aCGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGGTTTTTACtttttt                                                                                                                                         >  2:4490960/1‑148 (MQ=255)
             cGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGAc                                                                                                                                        <  2:1839135/150‑1 (MQ=255)
              gTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACg                                                                                                                                       <  1:376813/150‑1 (MQ=255)
               tCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTTCTGCTGCTTGTCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACGCTGTTATGGCC‑GGGATTTTATACTTTTGACGa                                                                                                                                      <  2:3482959/150‑1 (MQ=255)
               tCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTTCttttttcga                                                                                                                                      >  2:675026/1‑145 (MQ=255)
               tCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGCTTTATTCttttttcgg                                                                                                                                      >  2:2665876/1‑145 (MQ=255)
                 cAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGAtt                                                                                                                                    <  1:1909367/150‑1 (MQ=255)
                  aGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGAttt                                                                                                                                   <  2:4530425/150‑1 (MQ=255)
                   ggTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGGTTTTTTCTTTTTACGAtttt                                                                                                                                  >  2:336896/1‑149 (MQ=255)
                    gTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCa                                                                                                                                 <  1:1645988/150‑1 (MQ=255)
                    gTCTGGGGAAGGGGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCGGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGGTGACGCTGTTATGGCA‑GGGGTTTTATTATTTTGTCGAtttta                                                                                                                                 >  2:776987/1‑148 (MQ=255)
                     tCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGGTTTTTACTTTTTTCGATTTTAc                                                                                                                                >  2:4085480/1‑150 (MQ=255)
                      cTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTTCTTTTTACGGTTTTAcc                                                                                                                               >  2:4126403/1‑150 (MQ=255)
                      cTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGAGTTTATACTTTTGACGATTTCAcc                                                                                                                               >  1:3037607/1‑150 (MQ=255)
                       tGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTACTTTTTACGATTTCACCa                                                                                                                              >  1:1788151/1‑150 (MQ=255)
                       tGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCa                                                                                                                              <  1:1235880/150‑1 (MQ=255)
                         ggTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTACTTTTTTCGATTTTACCATg                                                                                                                            >  2:2120444/1‑150 (MQ=255)
                           tAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTATAATTTTTACGATTTTACCATGTc                                                                                                                          >  1:4461913/1‑150 (MQ=255)
                             aGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCgg                                                                                                                        <  2:1183604/150‑1 (MQ=255)
                               gtgtCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTa                                                                                                                      <  1:605688/150‑1 (MQ=255)
                               gtgtCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTa                                                                                                                      <  1:2545220/150‑1 (MQ=255)
                               gtgtCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTa                                                                                                                      <  2:1407227/150‑1 (MQ=255)
                                 gtCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAAc                                                                                                                    <  1:3555948/150‑1 (MQ=255)
                                 gtCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAAc                                                                                                                    <  2:701218/150‑1 (MQ=255)
                                       tttCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTTATTTTTTCGGTTTTTCCCTTTTGGTAACGCCGGa                                                                                                              >  2:214989/1‑150 (MQ=255)
                                         tcttctGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGGTTTATACCTTTGACGCTTTCACCATTTTGGTGACGCCGGaaa                                                                                                            >  1:774810/1‑149 (MQ=255)
                                          cttctGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACt                                                                                                           <  1:659432/150‑1 (MQ=255)
                                            tctGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTACTTTTTTCGATTTCACCATGTCGGGAACGCCGGGACTGa                                                                                                         >  1:1964130/1‑150 (MQ=255)
                                                gCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCt                                                                                                     <  1:4350709/150‑1 (MQ=255)
                                                   tAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTTCTTTTTTCGATTTTTCCATTTCGGGAACGCCCGGACTGTCGCTTGc                                                                                                  >  2:321520/1‑150 (MQ=255)
                                                   tAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGc                                                                                                  <  1:2730980/150‑1 (MQ=255)
                                                    aaTCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTTCTTTTTTCGATTTTACCCTTTCGGTAAAGCCGGGACTGACGcctgcg                                                                                                 >  2:1769232/1‑145 (MQ=255)
                                                    aaTCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTACTTTTTTCGATTTTACCCTGTCGGTAACGCCGGGACTGTCGCTGGCg                                                                                                 >  2:2475987/1‑150 (MQ=255)
                                                         ggggctggggctGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAatat                                                                                            <  1:625401/150‑1 (MQ=255)
                                                             ctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTATACTTTTTACGATTTTACCATGTCGGTAACGCCCGAACTGACGCTGGCGAATATTCGt                                                                                        >  1:2788065/1‑150 (MQ=255)
                                                             ctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTATACTTTTTACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGt                                                                                        >  1:3144271/1‑150 (MQ=255)
                                                             ctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGt                                                                                        <  1:1448546/150‑1 (MQ=255)
                                                             ctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGt                                                                                        <  1:2881138/150‑1 (MQ=255)
                                                              tggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTg                                                                                       <  1:249688/150‑1 (MQ=255)
                                                              tggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTg                                                                                       <  2:1586585/150‑1 (MQ=255)
                                                               ggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGGTTTATAGTTTTGACGATTTTACCATGGCGGGAAAGACGGGACAGAGGCTGGGGAataagcgggc                                                                                      >  2:750900/1‑143 (MQ=255)
                                                                  gctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCt                                                                                   <  1:152510/150‑1 (MQ=255)
                                                                   ctttttttGCTTCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTg                                                                                  <  2:1118420/147‑1 (MQ=255)
                                                                   ctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTg                                                                                  <  2:852514/150‑1 (MQ=255)
                                                                     gTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGtt                                                                                <  2:1878653/150‑1 (MQ=255)
                                                                        tttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTACTTTTTACGATTTTACCATGTCGGTAACGCCCGGACTGACGCCGGCGAATATCCGTGCGCTGtttta                                                                             >  2:4024627/1‑148 (MQ=255)
                                                                            ctgctgCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCg                                                                         <  1:3021292/150‑1 (MQ=255)
                                                                            ctgctgCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCAGGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCg                                                                         <  2:4398673/150‑1 (MQ=255)
                                                                            ctgctgCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGGTATGGCG‑GGGGATTTTTACATTTTTCGAATTTGCCCTGGCGGTGATGCCCGAAATTACGCGGGCCGATTTTCGGGCGCGGGTTaaagct                                                                         >  2:1223665/1‑146 (MQ=255)
                                                                               ctgctTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCg                                                                      <  2:3986326/150‑1 (MQ=255)
                                                                                tgctTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATCCTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGa                                                                     <  1:1696488/150‑1 (MQ=255)
                                                                                        ccGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTTCTTTTTACGATTTTACCCTGTTGGGAACCCCGGGAATGACGCTGGCGAATATTCCTGCGCTGTTTTATCCCGGGGATTTCGAc                                                             >  2:3615885/1‑150 (MQ=255)
                                                                                          gCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGAcat                                                           <  1:2124340/150‑1 (MQ=255)
                                                                                            tAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGGTTTTTACTTTTTTCGATTTCACCATGTCGGGAACGCCGGGAATGACGCTGGCGAATATCCGTGCGCTGTTTTATCCGGCGAATTACGAcatca                                                         >  2:2639999/1‑150 (MQ=255)
                                                                                             aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATGTTATGCTTTTGACGATTTCGCCATGCCGGTAACGCCGGAACTGACGCTGGTGAATATCCGTTCGCTGTTTAATCCGGCGAATTACGAcatcat                                                        <  2:2327664/150‑1 (MQ=255)
                                                                                              aTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCAtt                                                       <  1:4536554/150‑1 (MQ=255)
                                                                                                gtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATtct                                                     <  1:3587871/150‑1 (MQ=255)
                                                                                                  ggTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTACTTTTTTCGATTTTCCCCTGTCGGTAACCCCCGAAATGTCGCTGGCGGATATCCCTGCGCTGTTTTATTCGGCGGAATTCGACATCATTCTcc                                                   >  2:28804/1‑150 (MQ=255)
                                                                                                      tGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTACTTTTTACGATTTTACCATGTCGGTAACGCCGGGACTGACGCTGGCGAATATTCGTGCGCTGTTTTATTCGGCGAATTACGACATCATTCTCCGCAc                                               >  2:3694905/1‑150 (MQ=255)
                                                                                                      tGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCAc                                               <  2:430086/150‑1 (MQ=255)
                                                                                                      tGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCAc                                               <  1:4216430/150‑1 (MQ=255)
                                                                                                       ggCATTGTCTATATCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTTATTTTTTCAGATTTAACCTTG‑CAGGAAAACCGGTACTGACACTGGCGGATAAACGTGGGCTGGTTAATTCGGCGGAATTCGACAAAATTTTCCGCACg                                              >  2:4595768/1‑150 (MQ=255)
                                                                                                         cATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCt                                            <  2:3940455/150‑1 (MQ=255)
                                                                                                           ttGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTACTTTTTACGATTTTACCATGTCGGTAAAGGCCGAACTGACGCCGGCGGATATCCGTGCGCTGTTTTATTCGGCGAATTACGACATTATTCTCCCCCCCCTGa                                          >  2:296295/1‑150 (MQ=255)
                                                                                                            tGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGAc                                         <  2:1062672/150‑1 (MQ=255)
                                                                                                              tCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTACTTTTTACGATTTTACCATGTCGGGAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTTATCCGGCGAATTACGACATCATTCTCCCCCCGCCGACTa                                       >  2:3731547/1‑150 (MQ=255)
                                                                                                              tCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTTTACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTa                                       >  1:323422/1‑150 (MQ=255)
                                                                                                              tCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTa                                       <  2:1167193/150‑1 (MQ=255)
                                                                                                              tCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTa                                       <  1:2272447/150‑1 (MQ=255)
                                                                                                                tATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATg                                     <  2:1022052/150‑1 (MQ=255)
                                                                                                                 aTTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTACTTTTTTCGATTTTACCCTGTCGGTAACGCCGGGACTGACGCTGGGGAATATTCCTGCGCTGTTTTATCCGGCGAATTACGACATCATTCTCCGCCCGCTGGCTAttg                                    >  2:278036/1‑148 (MQ=255)
                                                                                                                  tttCGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTACTTTTTTCGATTTTACCCTGTCGGTAAAGCCCGGAATGACGCCGGGGAATATCCGCTGGCCGTTTTATTCGGCGAAATAAGGCATTATTCTCCGCCCCCTGACTATGGc                                   >  2:2112945/1‑150 (MQ=255)
                                                                                                                   ttCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCt                                  <  1:2335838/150‑1 (MQ=255)
                                                                                                                     cGGCTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTACTTTTTACGATTTTACCCTGTCGGTAACGCCCGGACTGACGCTGGGGAATATCCGTGCGCTGTTTTATCCCGCGAAATACGACATCATTTTCCGCCCGCTGACTATGGCtgt                                >  2:3522025/1‑150 (MQ=255)
                                                                                                                        cTCGCTGCTGACACTGTTATGGCA‑GGGGTTTTTTACTTTTTTCGGTTTCACCATGTCGGGAACGCCGGAACTTACGCTGGGGGATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGCCTATTGCTGTGGc                             >  2:1387919/1‑150 (MQ=255)
                                                                                                                          cGCTGCTGACACTGTTATGGCA‑GGGATTTTTTACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAg                           >  1:3294437/1‑150 (MQ=255)
                                                                                                                                 gACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTAt                    <  1:4042971/150‑1 (MQ=255)
                                                                                                                                 gACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTAt                    <  1:2599181/150‑1 (MQ=255)
                                                                                                                                 gACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTAt                    <  1:1395856/150‑1 (MQ=255)
                                                                                                                                    acTGTTATGGCA‑GGGGTTTTATACTTTTTACGATTTTACCCTGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTTATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGGCACTATCGc                 >  2:1509615/1‑150 (MQ=255)
                                                                                                                                     cTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGcc                <  1:1627495/150‑1 (MQ=255)
                                                                                                                                         tATGGCA‑GGGGTTTTTTACTTTTGACGATTTCACCATGTCGGTAACGCCGGGACTGACGCTGGCGAATATCCGTGCGCTGTTTTATCCGGCGAATTACGACATCATTTTCCGCACCCTGACTATGGCGGGGGCAGTCACTATCACCcgcg            >  2:2734091/1‑150 (MQ=255)
                                                                                                                                          aTGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAgcgc           <  2:2361552/150‑1 (MQ=255)
                                                                                                                                           tGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGcc          <  1:821966/150‑1 (MQ=255)
                                                                                                                                           tGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGcc          >  1:3867151/1‑150 (MQ=255)
                                                                                                                                              cA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCAtt       >  1:11846/1‑150 (MQ=255)
                                                                                                                                                  ggATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTg    >  1:4435391/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCA‑GGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGC  >  AP012306/1226410‑1226706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑