Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,421,1470TG34.6% 395.0 / 24.6 238T79P (ACC→CCC) ydhBpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (89/66);  new base G (82/0);  total (172/66)
Fisher's exact test for biased strand distribution p-value = 1.55e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 293 total aligned reads displayed.

TTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGAA  >  AP012306/1420998‑1421296
                                                                                                                                                     |                                                                                                                                                     
 tCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTg                                                                                                                                                      <  1:3319671/150‑1 (MQ=255)
   cTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTggt                                                                                                                                                    >  2:730155/1‑148 (MQ=255)
     gAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCt                                                                                                                                                  <  2:3096834/150‑1 (MQ=255)
     gAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCt                                                                                                                                                  >  2:4018671/1‑150 (MQ=255)
      aaaGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTg                                                                                                                                                 >  2:3073768/1‑150 (MQ=255)
       aaGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGc                                                                                                                                                >  2:1139929/1‑150 (MQ=255)
        aGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGCCCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCa                                                                                                                                               <  2:490205/150‑1 (MQ=255)
        aGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGCGCTGCa                                                                                                                                               >  1:723978/1‑150 (MQ=255)
          aCAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAtt                                                                                                                                             >  1:160266/1‑150 (MQ=255)
          aCAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCCtt                                                                                                                                             >  1:2210641/1‑150 (MQ=255)
               aagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCTCTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAttttttt                                                                                                                                        <  2:3206062/150‑1 (MQ=255)
               aagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGCCCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAttttttt                                                                                                                                        <  2:4097616/150‑1 (MQ=255)
               aagTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAttttttt                                                                                                                                        <  2:2147653/150‑1 (MQ=255)
                agTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATATGCATTTTTTTg                                                                                                                                       <  1:404994/150‑1 (MQ=255)
                agTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTTCAtttttttt                                                                                                                                       >  2:2225069/1‑149 (MQ=255)
                agTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCAtttttttt                                                                                                                                       >  1:2283686/1‑149 (MQ=255)
                  ttCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGac                                                                                                                                     >  1:3560785/1‑150 (MQ=255)
                   tCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCGTTTTTTTTaca                                                                                                                                    >  2:3803011/1‑150 (MQ=255)
                    cGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGCGAGCTTCACTTTTTTgtaaa                                                                                                                                   >  2:3662353/1‑146 (MQ=255)
                     gACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGacaac                                                                                                                                  >  1:3793076/1‑150 (MQ=255)
                      aCATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGCATTCCTTTTTTTTacaaca                                                                                                                                 >  2:2007489/1‑150 (MQ=255)
                       catcatCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACag                                                                                                                                >  1:2508883/1‑150 (MQ=255)
                        atcatcAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACaga                                                                                                                               <  1:2471290/150‑1 (MQ=255)
                        atcatcAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTtcaacaaa                                                                                                                               >  2:2084813/1‑148 (MQ=255)
                         tcatcaAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACagag                                                                                                                              <  1:4119405/150‑1 (MQ=255)
                            tcaAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAgcgc                                                                                                                           >  2:4355426/1‑150 (MQ=255)
                               aaaTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTGACAACAGAGCGCCct                                                                                                                        >  1:1400350/1‑150 (MQ=255)
                               aaaTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCct                                                                                                                        <  2:178564/150‑1 (MQ=255)
                                aaTGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTTCAACAGAGCGCCctt                                                                                                                       >  1:1809094/1‑150 (MQ=255)
                                  tGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCcttct                                                                                                                     <  1:3399583/150‑1 (MQ=255)
                                     cGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTg                                                                                                                  >  1:3908723/1‑150 (MQ=255)
                                     cGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGGCCTTCTTTg                                                                                                                  >  1:4363416/1‑150 (MQ=255)
                                         aaaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGaa                                                                                                              >  2:2304724/1‑150 (MQ=255)
                                         aaaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGaa                                                                                                              >  1:3020373/1‑150 (MQ=255)
                                          aaaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGaaa                                                                                                             >  1:4495131/1‑150 (MQ=255)
                                           aaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCGTTTTTTTTACAACAGAGGGCCCTTCTTTGAGAAAc                                                                                                            >  2:582256/1‑150 (MQ=255)
                                           aaaTCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTTAGAAAc                                                                                                            >  2:2841568/1‑150 (MQ=255)
                                               cAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCA‑TTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGc                                                                                                       <  1:1896119/150‑1 (MQ=255)
                                                    atcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCca                                                                                                   <  1:2496757/150‑1 (MQ=255)
                                                    atcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTTTGAAACCACGCTCca                                                                                                   >  2:2378582/1‑150 (MQ=255)
                                                     tcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCcag                                                                                                  >  1:2936200/1‑150 (MQ=255)
                                                     tcatcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCCTTTTTTTGACAACAGAGCGGCCTTCTTTGAGAAACCACGCTCcag                                                                                                  >  1:545362/1‑150 (MQ=255)
                                                      catcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTTAGAAACCACGCCCcagc                                                                                                 >  2:1698443/1‑150 (MQ=255)
                                                       atcTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCCTTTTTTTTGCAACAGAGCGCCCCTCTTTGTGAAACCCCGCTCcagcc                                                                                                >  2:3008102/1‑149 (MQ=255)
                                                         cTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCagcagc                                                                                              <  2:1211783/150‑1 (MQ=255)
                                                             cGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTc                                                                                          >  1:371241/1‑150 (MQ=255)
                                                              gtgtACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAAAGCGCCCCTCTTTTAGAAACCACGCCCCAGCAGCGGttc                                                                                         >  1:458202/1‑148 (MQ=255)
                                                              gtgtACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCa                                                                                         >  1:4194531/1‑150 (MQ=255)
                                                              gtgtACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAAAGCGCCCCTCTTTTAGAAAACACGCCCCCCCAGCGGGCa                                                                                         >  2:3394804/1‑150 (MQ=255)
                                                                 tACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCt                                                                                      <  1:2040798/150‑1 (MQ=255)
                                                                   cGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTTAGAAACCACCCTCCAGCAGCGGTCAGCTcc                                                                                    >  2:890911/1‑150 (MQ=255)
                                                                    gTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCCCGCCCCCCCAGCGGTCAGCACca                                                                                   >  2:1506679/1‑150 (MQ=255)
                                                                    gTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTTGCAACAGAGCCCCCCTCCTTGAGAAAACACCCTCCCGCAGCGGGCCGCTCca                                                                                   >  2:4120568/1‑150 (MQ=255)
                                                                     ttCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCcac                                                                                  <  1:156427/150‑1 (MQ=255)
                                                                      tCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCcaca                                                                                 >  1:3767585/1‑150 (MQ=255)
                                                                      tCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCcaca                                                                                 >  1:2894792/1‑150 (MQ=255)
                                                                      tCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGCTCTGCATTTTTTTGACAACAAAGGGCCCCTCTTTGAGAAACCACGCTCCAGCATCGGGCCACTCcacc                                                                                 >  2:809469/1‑149 (MQ=255)
                                                                       cTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGGTCTGCATTTTTTTTCAAACAGAGGGCCCTTCTTTTTGAAACCCCGCGCCAGGCGCGGTCAGGTCCGCAt                                                                                >  2:942175/1‑150 (MQ=255)
                                                                       cTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACAt                                                                                >  1:42975/1‑150 (MQ=255)
                                                                       cTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGGCAGCTCCACAt                                                                                >  1:2398731/1‑150 (MQ=255)
                                                                        tGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTGCAACAGAGCGCCCTTCTTTTAGAAAACACGCCCCAGCAGCGGTCAGCTCCACATc                                                                               >  2:4395714/1‑150 (MQ=255)
                                                                         ggCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTTACAACAAAGCGCCCTTCTTTGAGAAAACACGACCCAGGAGCGGGCAGCTCCCCATCa                                                                              >  2:3599037/1‑150 (MQ=255)
                                                                            cTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCCTTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGGCAACCCCACAACACgg                                                                           >  2:1897907/1‑150 (MQ=255)
                                                                            cTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTGCAACAAAGCGCCCTTCTTTGAGAAACCACGCCCCAGCAGCGGTCAGCCCCCCAACACgg                                                                           >  2:1046616/1‑150 (MQ=255)
                                                                             tGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCCTCTTTGAGAAAACACCCTCCAGCAGCGGTCAGCTCCACATccccgt                                                                          >  2:4504522/1‑145 (MQ=255)
                                                                             tGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAAAAGAGCGCCCTTCTTTGAGAAACCACGCCCCAGCAGCGGGCAGCTCCACATCACGGt                                                                          >  2:373850/1‑150 (MQ=255)
                                                                              gACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCCTCTTTTAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTg                                                                         >  2:3506926/1‑150 (MQ=255)
                                                                              gACAATATTATCCACTGCGATACCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTg                                                                         >  1:1351243/1‑150 (MQ=255)
                                                                               aCAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGc                                                                        >  1:212396/1‑150 (MQ=255)
                                                                                cAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGcc                                                                       <  1:108092/150‑1 (MQ=255)
                                                                                 aaTATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCcg                                                                      <  2:4424740/150‑1 (MQ=255)
                                                                                   tattatCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACCGTGCcgtc                                                                    >  1:3102585/1‑150 (MQ=255)
                                                                                    attatCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGCGGACTGCATTTTTTTGACCACAGAAGGCCCTTCTTTTAGCAACCCAGCCCCAACAACGGACAGGCCCCCATCACCGtttcgctg                                                                   >  2:1944192/1‑143 (MQ=255)
                                                                                      tatCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGtt                                                                 >  1:4238695/1‑150 (MQ=255)
                                                                                      tatCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGGGCGCCCTTCTTTGAGAAACCACGCTCCAGCCGCGGTCAGCTCCACATCACCGTGCCGTCGtt                                                                 >  2:1377420/1‑150 (MQ=255)
                                                                                          cACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTTAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCaaa                                                             >  2:1822012/1‑150 (MQ=255)
                                                                                          cACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCCCCAGCAGCGGTCAGCTCCACATCACGGGGCCGTCGTTCaaa                                                             >  2:3249239/1‑150 (MQ=255)
                                                                                          cACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGCCTGCACTTTTTTTGCAACAGGGCCCCCTTCTTTTTGGAACCACCCTCCAGCAGCGGGCCGCTCCCCCTCCCCGTGCCGTCGCTCaaa                                                             >  2:1652024/1‑150 (MQ=255)
                                                                                               cGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCg                                                        <  1:3433727/150‑1 (MQ=255)
                                                                                               cGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTGACAACAGAGCGCCCCTCTTTGAGAAACCACGCTCCCGCAGCGGGCAGCCCCACATCACCGTGCCCTCGGTCAAAAAGCg                                                        >  2:3921996/1‑150 (MQ=255)
                                                                                                   aGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTTCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCAc                                                    >  2:2604413/1‑150 (MQ=255)
                                                                                                        aCTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCccca                                               >  1:3193277/1‑150 (MQ=255)
                                                                                                         cTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCgcccg                                              >  2:2420340/1‑148 (MQ=255)
                                                                                                           gACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGGCAGCTCCACATCACCGTGCCGTCGTTCAAAGAGCGGCACCcccagcc                                            >  2:2635170/1‑150 (MQ=255)
                                                                                                           gACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCCCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCgccagcc                                            >  2:4254414/1‑150 (MQ=255)
                                                                                                           gACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACCGTGCCGTCGTTCCAAGAGCGGCCCcgcccgcc                                            >  2:1235508/1‑150 (MQ=255)
                                                                                                              cgcgCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCAct                                         >  1:323203/1‑150 (MQ=255)
                                                                                                                 gcCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCCTTTTTTTTGCCACAGAGCGCCCCTCTTTGTGGAAACACGCCCCCGCCACGGGCAGCCCCACCTCACGGTGCCGTCGTTCAAAAAGCGGCACCgccagccccccct                                      >  2:3884046/1‑144 (MQ=255)
                                                                                                                       cGTTCGCTATCTACTGACATTGCTGGCGGGGGGTCTGCATTTTTTTTACAAAAGAGCGCCCCTCTTTTAGAAAACACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGGCGTTCAAAAAGCGGCCCCCCCCGCCCCCCtttccacc                                >  2:4587247/1‑144 (MQ=255)
                                                                                                                        gTTCGCTATCTGCTGACATTGCTGGCGGGGGGTCTGCATTTTTTTTACAACAGAGCGGCCCTTTTTGGGAAACCACGCTCCAGCAGCGGTTAGCTCCACACCCCCGGGCCGTCGTTCAAAGAGCGGCACCGCCCGCCACCCTTCCCGCTg                               >  2:622677/1‑150 (MQ=255)
                                                                                                                         ttCGCTATCTGCTGACATTGCTGGCGGGGGATCTGCATTTTTTTTACAACAAAGCGGCCTTCTTTGAGAAACCCCGCCCCAGCCGCGGTTAGCTCCACATCCCGGTGCCCTCCTTCAAAAAGCGGCGCCGCCAGCCCCCCTTCCAGCAGa                              >  2:4176188/1‑150 (MQ=255)
                                                                                                                            gCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGc                           >  1:3574687/1‑150 (MQ=255)
                                                                                                                                    ctgACATTGCTGGCGGGGGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTTAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGtata                   >  1:2717418/1‑150 (MQ=255)
                                                                                                                                      gACATTGCTGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGGCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACCCTTCCCGCTGACGCACGGTATAGc                 >  2:2516464/1‑150 (MQ=255)
                                                                                                                                         aTTGCTGGCGGGGGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGa              >  1:2474620/1‑150 (MQ=255)
                                                                                                                                             cTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGc          >  1:1851746/1‑150 (MQ=255)
                                                                                                                                              tGGCGGGTGATCTGCATTTTTTTTACAACAGAGCGCCCTTCTTTGAGAAACCCCGCCCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCa         >  2:1988208/1‑150 (MQ=255)
                                                                                                                                                   ggTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAgg    >  1:2559927/1‑150 (MQ=255)
                                                                                                                                                    gTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGa   >  2:2780701/1‑150 (MQ=255)
                                                                                                                                                    gTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGa   >  1:788598/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCCAGCAGCGGTCAGCTCCACATCACGGTGCCGTCGTTCAAAGAGCGGCACCGCCAGCCACTCTTCCAGCTGACGCACGGTATAGCTGACCGCAGAAGGAA  >  AP012306/1420998‑1421296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑