Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,854,8510AC25.2% 780.9 / 57.6 311T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
Reads supporting (aligned to +/- strand):  ref base A (192/39);  new base C (0/78);  total (192/119)
Fisher's exact test for biased strand distribution p-value = 5.04e-44
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 346 total aligned reads displayed.

TAGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTGGGGCTG  >  AP012306/1854702‑1855000
                                                                                                                                                     |                                                                                                                                                     
taGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATaaaa                                                                                                                                                       <  1:2950528/150‑1 (MQ=255)
 aGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAAc                                                                                                                                                      <  2:3785167/150‑1 (MQ=255)
    ggTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAAcccc                                                                                                                                                   >  1:2926198/1‑150 (MQ=255)
    ggTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAAcccc                                                                                                                                                   >  1:2445137/1‑150 (MQ=255)
    ggTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAAcccc                                                                                                                                                   <  2:4016749/150‑1 (MQ=255)
     gTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCt                                                                                                                                                  <  2:2486788/150‑1 (MQ=255)
      tCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTg                                                                                                                                                 <  2:3222351/150‑1 (MQ=255)
        taataaCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGcc                                                                                                                                               >  1:730757/1‑150 (MQ=255)
        taataaCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGcc                                                                                                                                               >  1:2631014/1‑150 (MQ=255)
         aataaCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCg                                                                                                                                              >  1:474037/1‑150 (MQ=255)
          ataaCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGt                                                                                                                                             >  2:3873818/1‑150 (MQ=255)
           taaCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGtt                                                                                                                                            >  2:3646362/1‑150 (MQ=255)
             aCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTAt                                                                                                                                          >  1:4060856/1‑150 (MQ=255)
               aTCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCg                                                                                                                                        >  2:2016419/1‑150 (MQ=255)
               aTCCCTCAAGGGAAAAAGATCCATGGAATACTATTAGCAGAATAATCTCCCTACGCGAGACCATATTTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCCCGCTCACCCATTTTCGTCGTCATAAACCCCCTGCCGTTATCg                                                                                                                                        <  2:340971/150‑1 (MQ=255)
                tCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGt                                                                                                                                       >  1:800935/1‑150 (MQ=255)
                 cccTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGtg                                                                                                                                      <  1:4509789/150‑1 (MQ=255)
                  ccTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGtgc                                                                                                                                     >  2:224734/1‑150 (MQ=255)
                      aaGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGcc                                                                                                                                 >  2:1476881/1‑150 (MQ=255)
                      aaGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGcc                                                                                                                                 >  2:26713/1‑150 (MQ=255)
                       aGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGccc                                                                                                                                <  1:866343/150‑1 (MQ=255)
                           aaaaaGATCCATGGCATACTATTAGCAGAATAATCTCCCTACGCGAGACCATGATTTTTACCCTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCg                                                                                                                            <  1:115624/150‑1 (MQ=255)
                            aaaaGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCgg                                                                                                                           >  1:2594441/1‑150 (MQ=255)
                             aaaGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGgc                                                                                                                          >  1:1343224/1‑150 (MQ=255)
                              aaGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGgcg                                                                                                                         >  2:2837645/1‑150 (MQ=255)
                               aGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGgcgc                                                                                                                        <  1:3459167/150‑1 (MQ=255)
                                 aTCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGAAg                                                                                                                      <  1:2388749/150‑1 (MQ=255)
                                   ccATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGt                                                                                                                    >  2:1047274/1‑150 (MQ=255)
                                      tGGCATACTATTAGCAGAATAATCTCCCTACGCGAGACCATGATTTTTACCCTTCGCCCATATCAGAAAGAAGCCGTGGATGCCACGCTCACCCTTTTTCGTCGTAAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTaaa                                                                                                                 <  2:1183584/150‑1 (MQ=255)
                                      tGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTaaa                                                                                                                 >  2:3686652/1‑150 (MQ=255)
                                            aCTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAGGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTg                                                                                                           >  2:4091075/1‑150 (MQ=255)
                                            aCTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTg                                                                                                           >  2:537261/1‑150 (MQ=255)
                                             cTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTgg                                                                                                          >  1:17594/1‑150 (MQ=255)
                                                   gCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAg                                                                                                    <  1:3727218/150‑1 (MQ=255)
                                                    cAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGc                                                                                                   >  1:528215/1‑150 (MQ=255)
                                                     aGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCg                                                                                                  >  1:3192735/1‑150 (MQ=255)
                                                     aGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCg                                                                                                  >  1:3632761/1‑150 (MQ=255)
                                                      gAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCgg                                                                                                 >  2:350613/1‑150 (MQ=255)
                                                       aataatCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGa                                                                                                >  1:3918874/1‑150 (MQ=255)
                                                       aataatCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGa                                                                                                >  1:273059/1‑150 (MQ=255)
                                                          aatCTTCCCACGCGAGACCTTGATTTTTTCACTTCGCCCATATCAGCAAGAAGCGGGGGATGCCACGCTCACCCTTTTTCGCCGTAAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACt                                                                                             <  2:17323/150‑1 (MQ=255)
                                                            tCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTgg                                                                                           >  1:4402420/1‑150 (MQ=255)
                                                            tCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTgg                                                                                           >  2:693249/1‑150 (MQ=255)
                                                             ctacctacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGc                                                                                          >  1:4249665/1‑150 (MQ=255)
                                                              tacctccGCGAGACCATGATTTTTACCCTTCGCCCATATCAGCAAGAAGCCGTGGATGCCCCGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCa                                                                                         <  2:646233/150‑1 (MQ=255)
                                                               ccctacGCGAGACCAGGATTTTTACACTGCCCCATTATCAGCAAGAAGCCGTGAATGCCACGCTCAACCATTTCCTCCTTTATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCAc                                                                                        <  2:2081483/149‑1 (MQ=255)
                                                               acctacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCAc                                                                                        >  2:1283411/1‑150 (MQ=255)
                                                                cctacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACg                                                                                       <  1:3364291/150‑1 (MQ=255)
                                                                cctacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAATCCTGGTGATAGCGGAACTGGCACg                                                                                       >  1:1369349/1‑150 (MQ=255)
                                                                  tacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACggc                                                                                     >  1:654747/1‑150 (MQ=255)
                                                                    cgcgAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACggctg                                                                                   <  1:4181627/150‑1 (MQ=255)
                                                                    cgcgAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACggctg                                                                                   >  2:277797/1‑150 (MQ=255)
                                                                    cgcgAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACggctg                                                                                   >  2:4532540/1‑150 (MQ=255)
                                                                      cgAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGGTGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACggctggc                                                                                 >  2:1353934/1‑150 (MQ=255)
                                                                        agaCCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTc                                                                               >  1:1269177/1‑150 (MQ=255)
                                                                          aCCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGt                                                                             >  1:2141091/1‑150 (MQ=255)
                                                                            cATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTgg                                                                           >  2:114497/1‑150 (MQ=255)
                                                                              tGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTc                                                                         <  1:434130/150‑1 (MQ=255)
                                                                               gATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTGCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTcg                                                                        <  1:1006584/150‑1 (MQ=255)
                                                                                aTTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTcgc                                                                       >  2:4457862/1‑150 (MQ=255)
                                                                                aTTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTcgc                                                                       >  1:1057366/1‑150 (MQ=255)
                                                                                  ttttACACTTCGCCCATATCAGCAAGAAGCGGTGGATGCCACGCTCAACCATTTTCGTCGTAATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgt                                                                     <  2:1892625/150‑1 (MQ=255)
                                                                                   tttACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtg                                                                    >  2:299698/1‑150 (MQ=255)
                                                                                     tACACTCCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCGCACCCATTTTCGTCGACAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgct                                                                  <  2:4424790/150‑1 (MQ=255)
                                                                                             gCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGtgctggtgctgg                                                          >  1:931280/1‑150 (MQ=255)
                                                                                              cccATATCAGCAAGACGCCGTGGATGCCACGCTCAACCATTTCCGTCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGc                                                         <  2:863445/150‑1 (MQ=255)
                                                                                              cccATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGc                                                         >  1:4003456/1‑150 (MQ=255)
                                                                                                cATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGcac                                                       >  2:2584633/1‑150 (MQ=255)
                                                                                                     cAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGtt                                                  >  2:2932576/1‑150 (MQ=255)
                                                                                                      acaaagacgCCGGGGTTCCCACCCTCACCCTTTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTa                                                 <  2:959741/147‑1 (MQ=255)
                                                                                                      aGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTa                                                 >  1:3103920/1‑150 (MQ=255)
                                                                                                      aGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGGCGCGTGCTGGTGCTGGCACACGTTa                                                 >  2:757517/1‑150 (MQ=255)
                                                                                                      aGCAAGAAGCCGTGGAGGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTa                                                 <  1:4136564/150‑1 (MQ=255)
                                                                                                       gCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTaa                                                >  2:2382957/1‑150 (MQ=255)
                                                                                                       gCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTaa                                                <  1:1946604/150‑1 (MQ=255)
                                                                                                        cAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGGTaaa                                               >  2:289549/1‑150 (MQ=255)
                                                                                                        cAAGAAGCCGTGGATGCCACGCCCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTaaa                                               <  1:943409/150‑1 (MQ=255)
                                                                                                         aagaagCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAg                                              >  2:544784/1‑150 (MQ=255)
                                                                                                                cGTGGATGCCACGCTCACCCATTTTCGTCGTCATAACCCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACtggt                                       <  2:3124948/150‑1 (MQ=255)
                                                                                                                  tGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACtggtgg                                     >  1:3128939/1‑150 (MQ=255)
                                                                                                                   ggATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGgc                                    <  1:3592505/150‑1 (MQ=255)
                                                                                                                    gATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGgcg                                   <  1:2087184/150‑1 (MQ=255)
                                                                                                                    gATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGgcg                                   >  1:2310281/1‑150 (MQ=255)
                                                                                                                                  cgaTTTGCGGCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCaaa                     <  1:2013785/148‑1 (MQ=255)
                                                                                                                                  cctttttCGTGGCAAAAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCaaa                     <  2:2513483/147‑1 (MQ=255)
                                                                                                                                  ccATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCaaa                     >  2:1531030/1‑150 (MQ=255)
                                                                                                                                  ccATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCaaa                     >  1:1231821/1‑150 (MQ=255)
                                                                                                                                       ttCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATc                <  1:1848811/150‑1 (MQ=255)
                                                                                                                                       ttCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATc                >  1:4091001/1‑150 (MQ=255)
                                                                                                                                          gtcgtcATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAgg             >  1:3780726/1‑150 (MQ=255)
                                                                                                                                           tcttaaTAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGgc            <  2:1827464/145‑1 (MQ=255)
                                                                                                                                           tcgtcAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGgc            <  2:2123121/150‑1 (MQ=255)
                                                                                                                                             gtcATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGgcgc          <  1:2219676/150‑1 (MQ=255)
                                                                                                                                              tcATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCt         <  2:4284557/150‑1 (MQ=255)
                                                                                                                                               caaaaaaCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTg        <  2:581370/147‑1 (MQ=255)
                                                                                                                                                aaaaacCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTgg       <  2:4342559/148‑1 (MQ=255)
                                                                                                                                                aTAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTgg       >  2:2444745/1‑150 (MQ=255)
                                                                                                                                                 tAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTggg      <  1:4432975/150‑1 (MQ=255)
                                                                                                                                                  aaacCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTgggg     <  1:1625867/150‑1 (MQ=255)
                                                                                                                                                  aaaaCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTgggg     >  1:1489760/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTGGGGCTG  >  AP012306/1854702‑1855000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑