Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,212,7530AC25.5% 362.4 / 38.3 145Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base A (99/9);  new base C (0/37);  total (99/46)
Fisher's exact test for biased strand distribution p-value = 2.50e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 193 total aligned reads displayed.

CAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGTG  >  AP012306/2212604‑2212901
                                                                                                                                                     |                                                                                                                                                    
cAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATa                                                                                                                                                      <  1:3492946/150‑1 (MQ=255)
 aaCGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATAc                                                                                                                                                     >  1:1195488/1‑150 (MQ=255)
    gCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATAcccc                                                                                                                                                  >  1:92444/1‑150 (MQ=255)
     ccGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACACAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCa                                                                                                                                                 <  2:350114/150‑1 (MQ=255)
       gTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCAcc                                                                                                                                               <  1:739558/150‑1 (MQ=255)
              cTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCgccgagg                                                                                                                                        >  1:2287210/1‑150 (MQ=255)
                    tAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGt                                                                                                                                  >  2:1480250/1‑150 (MQ=255)
                    tAATGCCCAAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGt                                                                                                                                  <  2:252540/150‑1 (MQ=255)
                     aaTGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCCCCccccaggcccagga                                                                                                                                 >  2:2206616/1‑148 (MQ=255)
                          cccAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACCAATACCCCACCGCCGAGGCCGAGTAGCCAg                                                                                                                            >  1:3666913/1‑150 (MQ=255)
                          cccAGCCCACTACGCCGAAAACCGCGGAGATCAACAGCATATCGCGTGCACTACAACGGCGAAAAAGTTTATTCCCCACCGAAAAGATAATGACTTCCGCCCCCCCGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAg                                                                                                                            <  2:1009360/150‑1 (MQ=255)
                            cAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGct                                                                                                                          <  1:517006/150‑1 (MQ=255)
                              gcccACTACGCCGCAAATCGCCAAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTCCTCAGCGCAAAGATAATGACTCCCGCCCCCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGctgc                                                                                                                        <  2:501048/147‑1 (MQ=255)
                                 cACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGctgcctg                                                                                                                     >  1:1820041/1‑150 (MQ=255)
                                   cTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCCAGTAGCCAGCtgcctgcc                                                                                                                   >  2:2322257/1‑150 (MQ=255)
                                   cTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGGTTATTACCCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCtgcctgcc                                                                                                                   >  2:1757464/1‑150 (MQ=255)
                                   cTACGCCGCAAATCGCCGAGATCAACAGCATACCGCGTGCACTACAAGGGCGGAAAATTTTATTACCCAGCGAAAAGATAATGACTTCCGCCCCCCCGCCCAGCGCCCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCtgcctgcc                                                                                                                   <  2:2483751/150‑1 (MQ=255)
                                    tACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCa                                                                                                                  >  1:4287345/1‑150 (MQ=255)
                                         cgaaaaTCGCCGAGACCAACAGCATATCGCGTGCACTACAACGGCGAAAAATTTTATTACCCAGCGAAAAGAAAATGACTCCCGCCCCCACGCCCAGCGACCAAAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTaaa                                                                                                             <  2:4360719/147‑1 (MQ=255)
                                            aaaTCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATgg                                                                                                          >  1:3468153/1‑150 (MQ=255)
                                            aaaTCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATgg                                                                                                          >  2:2885682/1‑150 (MQ=255)
                                                cGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCt                                                                                                      >  1:1319299/1‑150 (MQ=255)
                                                  ccGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTaa                                                                                                    >  2:3218289/1‑150 (MQ=255)
                                                   cGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTaaa                                                                                                   >  1:2479262/1‑150 (MQ=255)
                                                     agaCCAACACCATACCGCGTCCACTACACCGGCGAAAAATTTTATTCCCCACCCCAAAAATATTGCCTCCCCCCCCCCCGCCCAGCGCCCAAAAACCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAAc                                                                                                 <  2:3118411/150‑1 (MQ=255)
                                                       aTCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAACCAGCTGCCTGCCAGTAAATGGCGCTAAAACCg                                                                                               >  2:1907475/1‑150 (MQ=255)
                                                         cAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTa                                                                                             >  2:2359184/1‑150 (MQ=255)
                                                         cAACAGCATATCGCGTGCACTACAACGGCGGAAAAGGTTTTTACCCAGCGCAAAAAAAATGAATTCCGCCACCACGCCCAGCGACCACAAATACCCCAACCCCAAGGCCCAAAAGCCAGCAGCCCGCCAGTAAAATGCGCTAAAAcccaa                                                                                             >  2:3387487/1‑147 (MQ=255)
                                                          aaCAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTaa                                                                                            >  1:2563795/1‑150 (MQ=255)
                                                          aaCAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTaa                                                                                            >  1:2519951/1‑150 (MQ=255)
                                                           ccAGCATATCGCGCGCCCCACAACGGCGAAAAAGTTTATTCCCCAGCGAAAAAATAATGACTCCCCCCCCCACGCCCAGGGACCACAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAAt                                                                                           <  2:597379/149‑1 (MQ=255)
                                                           acacCATATCGCGTGCACTACACCGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCCCGCCCAGCGACCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAAt                                                                                           <  1:184629/150‑1 (MQ=255)
                                                           aCAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAAt                                                                                           >  1:1637381/1‑150 (MQ=255)
                                                           aCAGAATATCGCGCGCACTACAGCGCCGGAAAATTTTATTACACAGCGCAAGAAAAGCGCCCCCCGCCCCCCCGCCGAGCGCCCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAAt                                                                                           <  2:3132326/150‑1 (MQ=255)
                                                            cAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATa                                                                                          >  2:3483684/1‑150 (MQ=255)
                                                               cATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGc                                                                                       >  1:1137349/1‑150 (MQ=255)
                                                                  atCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGc                                                                                    <  1:3005199/150‑1 (MQ=255)
                                                                  atCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGc                                                                                    >  1:4102501/1‑150 (MQ=255)
                                                                         gCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATAACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGATGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAAAAGGCCGCATGTGcc                                                                             >  2:1402712/1‑150 (MQ=255)
                                                                          cACTACAACGGCGAAAAAGTTTATTACTCAGCGAAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGccc                                                                            <  1:297102/150‑1 (MQ=255)
                                                                             tACACCGGCGGAAAATTTTATTCCCCAGCGAAAAGATAATGACTCCCGCCACCCCGCCCAGCGCCCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCt                                                                         <  2:848312/150‑1 (MQ=255)
                                                                                 aCGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTCCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCaa                                                                     <  1:4164227/150‑1 (MQ=255)
                                                                                  cggcgaAAAATTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAAt                                                                    <  1:4386650/150‑1 (MQ=255)
                                                                                   ggggaaaaaGTTTATTCCTCAGCGAAAAGAAAATGCCTCCCGCCCCCCCCCCCAGCGCCCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATa                                                                   <  2:1578998/145‑1 (MQ=255)
                                                                                   ggcgaAAAATTTTATTCCTCAGCGAAAAGATAATGTCTCCCGCCCCCCCGCCCAGCGACCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATa                                                                   <  2:784971/150‑1 (MQ=255)
                                                                                   ggcgaAAAAGTTTATTCCACAGCCAAAAGAAAAGGACTTCCCCCACCACGCCCGGGGACCAAAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATa                                                                   <  2:184850/150‑1 (MQ=255)
                                                                                    gcggAAAATTTAATAACTCAGCGAAAAAAAAAGACCTCCCGCCACCCCGCCCAGCGACCAAAAACCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaa                                                                  <  2:1715188/150‑1 (MQ=255)
                                                                                    gcgaAAAATTTTATTCCTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaa                                                                  <  1:1966625/150‑1 (MQ=255)
                                                                                     cggAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaa                                                                 >  2:888048/1‑150 (MQ=255)
                                                                                     cggAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaa                                                                 >  2:2639797/1‑150 (MQ=255)
                                                                                     cggAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaa                                                                 >  1:3294976/1‑150 (MQ=255)
                                                                                         aaaGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaaa                                                             >  1:1311862/1‑150 (MQ=255)
                                                                                            gTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGc                                                          >  1:4594716/1‑150 (MQ=255)
                                                                                            gTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGc                                                          >  1:1768815/1‑150 (MQ=255)
                                                                                             tttATTCCCCACCCCAAAGATAATGCCTCCCCCCCCCCCCCCCAGCCCCCACAAACCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCa                                                         <  2:3607708/150‑1 (MQ=255)
                                                                                               tattaCTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAgg                                                       >  1:3528180/1‑150 (MQ=255)
                                                                                               tattaCTCAGCGCAAAGATAATGACTCCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAgg                                                       <  1:4011673/150‑1 (MQ=255)
                                                                                                  taCTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCa                                                    >  2:4614351/1‑150 (MQ=255)
                                                                                                  taCTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCa                                                    >  1:2919562/1‑150 (MQ=255)
                                                                                                   acacAGCGAAAAGATAATGACTCCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAg                                                   <  1:1938273/147‑1 (MQ=255)
                                                                                                      cAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGaaa                                                >  2:2563524/1‑150 (MQ=255)
                                                                                                      cAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGaaa                                                >  2:3748636/1‑150 (MQ=255)
                                                                                                       accgcAAAGATACCGCCTCCCGCCCCCACCCCCAGCGCCCAAAAACCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAg                                               <  2:3602871/148‑1 (MQ=255)
                                                                                                          gcAAAGATAATGACTTCCGCCCCCACGCCCAGCGACCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAgcgc                                            <  1:1475093/150‑1 (MQ=255)
                                                                                                          gcAAAGATAATACCTCCCGCCCCCCCCCCCGGCGACCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAgcgc                                            <  2:548758/150‑1 (MQ=255)
                                                                                                            aacgATAATGTCTTCCGCCACAACGCCGAGCGCCCACAAACCCCCCCCCGCCGAGGCCGGGTAGCCAGCTGCCTGCCAGTAAATGGCGTTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAgcgcta                                          <  2:1857153/147‑3 (MQ=255)
                                                                                                            aaaGAAAATACCTTCCCCCCCCCCCCCCAGCGCCCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCa                                          <  2:3952199/150‑1 (MQ=255)
                                                                                                              aGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGt                                        >  1:1779918/1‑150 (MQ=255)
                                                                                                              aGAAAATGACTCCCGCCACCACGCCAAGGACCCAAAAATACCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGt                                        <  1:4184208/150‑1 (MQ=255)
                                                                                                               gATAATGCCTCCCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGtt                                       <  1:1895355/150‑1 (MQ=255)
                                                                                                                aTAATGCCTCCCGCACCCACGCCCAGCGACCAAAAAGCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTc                                      <  2:960574/150‑1 (MQ=255)
                                                                                                                 tAATGCCTCCCGCCCCCCCCCCCGGCGCCCAAAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCt                                     <  2:2077970/150‑1 (MQ=255)
                                                                                                                 tAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCt                                     >  1:4370008/1‑150 (MQ=255)
                                                                                                                  aaTGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTg                                    >  1:4493846/1‑150 (MQ=255)
                                                                                                                   gcttctcccGCCACCCCCACCGGCACCCCCAAAACCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTgg                                   <  2:2719782/143‑1 (MQ=255)
                                                                                                                     gcctcccGCCACCACGCCAAGCGCCCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCg                                 <  2:4022769/145‑1 (MQ=255)
                                                                                                                     gACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCg                                 >  2:2603597/1‑150 (MQ=255)
                                                                                                                     gACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCg                                 >  1:4520925/1‑150 (MQ=255)
                                                                                                                        ttCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAAc                              >  1:4121887/1‑150 (MQ=255)
                                                                                                                        ttCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAAc                              >  2:3846185/1‑150 (MQ=255)
                                                                                                                         cccGCCACCCCGCCCAGCGCCCACAAACCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAAcc                             <  2:1680076/149‑1 (MQ=255)
                                                                                                                          ccGCCACCCCGCCCAGCGCCCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCa                            <  2:1283493/150‑1 (MQ=255)
                                                                                                                          ccGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCa                            >  1:870317/1‑150 (MQ=255)
                                                                                                                           cGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAg                           <  1:3270365/150‑1 (MQ=255)
                                                                                                                           cGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAg                           >  1:4433938/1‑150 (MQ=255)
                                                                                                                           cGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAg                           >  1:3593370/1‑150 (MQ=255)
                                                                                                                            gCCACCCCCCCCGGCGACCACAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgc                          <  2:3037688/150‑1 (MQ=255)
                                                                                                                            gCCACCACGCCCAGCGCCCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgc                          <  1:3856489/150‑1 (MQ=255)
                                                                                                                            gCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgc                          >  1:468541/1‑150 (MQ=255)
                                                                                                                              ccccccccccAGCGCCCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgcgc                        <  2:2003606/143‑1 (MQ=255)
                                                                                                                                    gCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCa                  <  2:240574/150‑1 (MQ=255)
                                                                                                                                    gCCCAGCGACCACAAATACCCCACCGCCGAGGCCTAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCa                  >  1:1263181/1‑150 (MQ=255)
                                                                                                                                    gCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCa                  >  2:3262578/1‑150 (MQ=255)
                                                                                                                                        aGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCa              >  1:375731/1‑150 (MQ=255)
                                                                                                                                          cGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGa            <  1:2844693/150‑1 (MQ=255)
                                                                                                                                           gACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGAc           >  1:3992781/1‑150 (MQ=255)
                                                                                                                                               acaAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAAc       <  1:1340174/150‑1 (MQ=255)
                                                                                                                                                caAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAAcc      <  1:305753/150‑1 (MQ=255)
                                                                                                                                                caAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAAcc      >  1:1541521/1‑150 (MQ=255)
                                                                                                                                                    tACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGTg  >  1:3816799/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGTG  >  AP012306/2212604‑2212901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑