Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,020,6700TG26.0% 385.8 / 15.2 196K195N (AAA→AAC) glgCglucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (94/51);  new base G (51/0);  total (145/51)
Fisher's exact test for biased strand distribution p-value = 1.50e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 278 total aligned reads displayed.

ATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTCTTGCT  >  AP012306/3020521‑3020818
                                                                                                                                                     |                                                                                                                                                      
 tCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATGCTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGtt                                                                                                                                                       <  1:2021314/150‑1 (MQ=255)
  cAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGttt                                                                                                                                                      <  2:365794/150‑1 (MQ=255)
   aaaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGtttt                                                                                                                                                     <  2:3355387/150‑1 (MQ=255)
   aaaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGGAGGgttt                                                                                                                                                     >  2:2259872/1‑150 (MQ=255)
    aaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGgtttt                                                                                                                                                    >  2:446214/1‑150 (MQ=255)
    aaTCTTTGCCAAAGTCGTGGCTGGAGTTCCCATCGCGATGGGCTTCTTCCAGCCCTTCAGACAGATAGTCAGCGGCAAAGACGAATATACCCATACCCGCCAGAGCTTTGCTCGTACCGCTCTGCATTGACGGCCGGCTATCGCGttttt                                                                                                                                                    >  2:1061479/1‑150 (MQ=255)
     aTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTc                                                                                                                                                   >  1:358837/1‑150 (MQ=255)
       cTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTTGCCGGGTTTTCaa                                                                                                                                                 >  2:2964467/1‑150 (MQ=255)
          tGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGa                                                                                                                                              <  2:811884/150‑1 (MQ=255)
          tGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGATTTTCAACGa                                                                                                                                              >  1:1631448/1‑150 (MQ=255)
           gCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGaa                                                                                                                                             >  1:4467667/1‑150 (MQ=255)
             cAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑tt                                                                                                                                          <  2:504680/150‑1 (MQ=255)
                aGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAAGGTTTTCAACGAA‑ATCGa                                                                                                                                       >  2:152338/1‑150 (MQ=255)
                 gTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGAt                                                                                                                                      <  1:1457671/150‑1 (MQ=255)
                  tCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATa                                                                                                                                     >  1:1931055/1‑150 (MQ=255)
                     tGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATATTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAAtt                                                                                                                                  <  2:4138856/150‑1 (MQ=255)
                     tGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAAtt                                                                                                                                  <  1:3463239/150‑1 (MQ=255)
                      ggCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAAttt                                                                                                                                 <  1:2971571/150‑1 (MQ=255)
                       gCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAAtttt                                                                                                                                <  1:1750577/150‑1 (MQ=255)
                       gCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTCAACGAA‑TTCGATAAtttt                                                                                                                                >  1:972007/1‑150 (MQ=255)
                        cTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTa                                                                                                                               >  1:1203858/1‑150 (MQ=255)
                         tGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑t                                                                                                                             <  2:3375924/150‑1 (MQ=255)
                         tGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑t                                                                                                                             <  2:2586863/150‑1 (MQ=255)
                            aGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAA‑TTCGATAATTTTT‑TCGt                                                                                                                          >  2:1994709/1‑150 (MQ=255)
                             gTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTTAACGAA‑TTCGATAATTTTA‑TCGtt                                                                                                                         >  2:2089271/1‑150 (MQ=255)
                              ttCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTtc                                                                                                                        <  1:2755081/150‑1 (MQ=255)
                              ctcgaaTCCCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGGCGTAGATGCCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTtc                                                                                                                        <  2:595476/145‑1 (MQ=255)
                                ctcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTTAACGAA‑TTCGATAATTTTT‑TCGTtttc                                                                                                                      >  2:2159744/1‑150 (MQ=255)
                                 tcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCtca                                                                                                                     >  2:2791159/1‑150 (MQ=255)
                                 tcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCtca                                                                                                                     <  2:1842668/150‑1 (MQ=255)
                                     cgcgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCaa                                                                                                                 <  2:3593857/150‑1 (MQ=255)
                                      gcgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAAttt                                                                                                                                 >  2:923667/1‑134 (MQ=255)
                                      gcgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAAc                                                                                                                <  1:3827861/150‑1 (MQ=255)
                                      gcgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAAc                                                                                                                <  1:256732/150‑1 (MQ=255)
                                       cgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAAcc                                                                                                               <  1:58130/150‑1 (MQ=255)
                                        gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCg                                                                                                              <  2:2323857/150‑1 (MQ=255)
                                        gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCg                                                                                                              >  2:245267/1‑150 (MQ=255)
                                         aTCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGc                                                                                                             <  1:3338637/150‑1 (MQ=255)
                                          tCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTTAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAAACGcc                                                                                                            >  2:1728099/1‑150 (MQ=255)
                                            gTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTTGGTAATTTTT‑TTTTTCTCATCAACCGCCAt                                                                                                          >  2:1578991/1‑150 (MQ=255)
                                                    ccAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCaa                                                                                                  >  1:3177261/1‑150 (MQ=255)
                                                      agcagTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAAt                                                                                                >  2:3919199/1‑150 (MQ=255)
                                                           ttCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGa                                                                                           >  2:1923902/1‑150 (MQ=255)
                                                             cATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAgg                                                                                         >  1:788883/1‑150 (MQ=255)
                                                                aaaGATAGCCGGCGCAAAAGACAAAGATCCCCATCCCCCCCAGATTTTTGCTCGGATCTTCCGGCATGGGCGGCGGTTTAGCAGTTTTTACAACAAA‑TTCGATATTTTTT‑TCTTTCAAACCACCCGCCATAACGCCAAATGCGGAGGctt                                                                                      <  2:4248144/148‑1 (MQ=255)
                                                                aCAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCCAATGCGGAGGctt                                                                                      >  1:1082085/1‑150 (MQ=255)
                                                                aCAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGctt                                                                                      >  1:3052092/1‑150 (MQ=255)
                                                                 cAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGcttc                                                                                     >  1:1540408/1‑150 (MQ=255)
                                                                    aTAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCttcttc                                                                                  >  1:2427740/1‑150 (MQ=255)
                                                                        tCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGCTAGCAAGGTTTTCAACAAA‑TTCAATAACTTTA‑TCCTTTTCATCAACCCCCAAAACCCCCAAATCGGGCGCCTTTTCAAtt                                                                              >  2:4342122/1‑149 (MQ=255)
                                                                          ggCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCCTAACGCCAAATGCGGAGGCTTCTTCAATCgg                                                                            >  2:69250/1‑150 (MQ=255)
                                                                           gCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGt                                                                           >  1:3928922/1‑150 (MQ=255)
                                                                              tCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACt                                                                        >  1:508471/1‑150 (MQ=255)
                                                                              tCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTCAACGGA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACt                                                                        >  1:3243718/1‑150 (MQ=255)
                                                                              caaaaGATGTAGATCCCAATCCTCGCCAGAGTTTTGCTGGGATCGTTCGGCATTGGCGG‑GGGTTAAGAGGTTTTTTAAAAAATTTAGATATTTTTT‑TCTTTCTCACAAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACt                                                                        <  2:342946/148‑1 (MQ=255)
                                                                                aaaGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAAAGCCAAATGCGGAGGCTTCTTCAATCGGTACttg                                                                      >  2:431120/1‑148 (MQ=255)
                                                                                  aGACGTAGATACACATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCa                                                                    >  2:1316615/1‑150 (MQ=255)
                                                                                      gTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACa                                                                >  1:2352586/1‑150 (MQ=255)
                                                                                       tAGATCCCCATCCTCGCAAGAGATTTGCTGGTATCGTTCGGCATTGACGGGGGGTTAGCAGTTTTTAAAACAAA‑TTCAATATTTTTA‑TCTTTCTCACCACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACaa                                                               <  2:3519310/150‑1 (MQ=255)
                                                                                       tAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACaa                                                               >  1:4140256/1‑150 (MQ=255)
                                                                                       tAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACaa                                                               >  2:3751548/1‑150 (MQ=255)
                                                                                       tAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACaa                                                               >  2:2537366/1‑150 (MQ=255)
                                                                                        aGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTTAACGAA‑ATCGATAATTTTT‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCCTCTTCAATCGGTACTTGCATACAAg                                                              >  1:2300773/1‑150 (MQ=255)
                                                                                         gATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGc                                                             >  1:3968457/1‑150 (MQ=255)
                                                                                          aTACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGGAGGGGTTTCAACGAA‑TTCGATAATTTTA‑TGGTTTTCATCAACCGGCATAACGCCAAAAGCGGAGGCTTCTTCAATTGGTACTGGCATACAAGCa                                                            >  2:598977/1‑150 (MQ=255)
                                                                                            aCCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAA‑TTTGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCCTCTTCAATCGGTACTGGCATTCAAGCAAc                                                          >  1:896721/1‑150 (MQ=255)
                                                                                            aCCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAAc                                                          >  1:4103100/1‑150 (MQ=255)
                                                                                                aTACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTTAACGCA‑TTCGATAATTTTA‑TCGGTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGGACTGGCATACAAGCAACgggg                                                      >  2:1128009/1‑148 (MQ=255)
                                                                                                  aCTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGATAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCCAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAAACGTGCa                                                    >  1:2263401/1‑150 (MQ=255)
                                                                                                   cTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAACAACGGTGCaa                                                   >  1:2112779/1‑150 (MQ=255)
                                                                                                    tCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAAc                                                  >  1:4160436/1‑150 (MQ=255)
                                                                                                      gCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGt                                                >  1:179927/1‑150 (MQ=255)
                                                                                                         agagATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTgcg                                             >  1:3821758/1‑150 (MQ=255)
                                                                                                           agaTTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGcc                                           >  1:1012972/1‑150 (MQ=255)
                                                                                                            gaTTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCt                                          >  1:4541537/1‑150 (MQ=255)
                                                                                                               ttGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCtttt                                       >  1:4590427/1‑150 (MQ=255)
                                                                                                               ttGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACCAA‑TTCCATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGGGGCTTCTTCAATCGGTACTGGCATACAAGCAACCGTGCAACGTGCGCCtttt                                       >  2:2473609/1‑150 (MQ=255)
                                                                                                                  cTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TAGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCg                                    >  2:2809541/1‑150 (MQ=255)
                                                                                                                     ggctCGTTCGCCATGGCGGGGGGGTTAGGGGTTTTTAAAACGAA‑TTGAATAATTTTA‑TCGTTCTCAT‑AACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGt                                <  2:2826883/147‑1 (MQ=255)
                                                                                                                        tCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGa                              >  2:2976263/1‑150 (MQ=255)
                                                                                                                           ttCGGCATGGGGGGGGGGTTAGCATTTTTTAAAACAAA‑TTCAATATTTTTT‑TCTTTCTAACCACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCg                           <  2:1783398/150‑1 (MQ=255)
                                                                                                                             gggCATGGGCGGGGGGTAAGCGGTTTTTCCAACAAA‑TTCATTATTTTTT‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTTCTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGAt                         <  2:1613869/149‑1 (MQ=255)
                                                                                                                              ggCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATa                        >  1:3289188/1‑150 (MQ=255)
                                                                                                                               gaatggACGGGGGGTTAGCAGTTTTTTCAAAAAA‑TTCAATATTTTTA‑TCTTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATaa                       <  2:2044036/145‑1 (MQ=255)
                                                                                                                               gCATTGGCGGGGGTTTGGGGGGTTTTAAACCGAT‑TCCGATAATTTTT‑CCGTTCTCAAAAGCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATaa                       <  1:4275138/150‑1 (MQ=255)
                                                                                                                               gCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATaa                       >  1:740084/1‑150 (MQ=255)
                                                                                                                                 aTTGACGGCGGGGTAGCAGGGTTTTTAACGAA‑TTCGATAATTTTT‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGc                     >  2:3438977/1‑150 (MQ=255)
                                                                                                                                   tGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGCGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCAt                   >  1:700712/1‑150 (MQ=255)
                                                                                                                                    gACGGGGGTTTAGCGGTTTTTTCAACGAA‑TTCGATATTTTTA‑TCTTTCTAATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATa                  <  1:3665040/150‑1 (MQ=255)
                                                                                                                                      cggcggGTTAGCAGGGTTTTCAACGAA‑ATCGATAATTTTA‑TCGGTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATAcg                >  1:4394353/1‑150 (MQ=255)
                                                                                                                                          gtattAGTAGTTTTTTCAAC‑AA‑TTTCATTTTTTTATTCTTTCCCATAAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGag            <  2:1686639/147‑1 (MQ=255)
                                                                                                                                          ggttgaGCAGTTTTTACAACGAA‑TTAAATAATTTTT‑TCGTTCTCAACAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGag            <  2:4030155/145‑1 (MQ=255)
                                                                                                                                           ggTTAGCAGTTTTTTCAAAGAT‑TCCGATATTTTTT‑TCGTTCTCATCACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagt           <  2:2060266/150‑1 (MQ=255)
                                                                                                                                              tAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagtagt        >  1:2935496/1‑150 (MQ=255)
                                                                                                                                              tAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagtagt        >  1:2514152/1‑150 (MQ=255)
                                                                                                                                              tAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagtagt        >  1:1705661/1‑150 (MQ=255)
                                                                                                                                               aGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTc       >  1:1493799/1‑150 (MQ=255)
                                                                                                                                                gCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTct      >  1:1352130/1‑150 (MQ=255)
                                                                                                                                                  aGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttg    >  2:2710501/1‑150 (MQ=255)
                                                                                                                                                   tttttttCAACGAA‑TTAGAAATTTTTT‑TCGTTTTAACAGACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttgc   <  2:3004560/148‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
ATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAA‑TTCGATAATTTTA‑TCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTCTTGCT  >  AP012306/3020521‑3020818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑