Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,372,7010TG27.7% 121.2 / 25.6 65noncoding (54/76 nt)gltUtRNA‑Glu
Reads supporting (aligned to +/- strand):  ref base T (1/46);  new base G (18/0);  total (19/46)
Fisher's exact test for biased strand distribution p-value = 3.81e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 130 total aligned reads displayed.

CACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAA  >  AP012306/3372553‑3372758
                                                                                                                                                      |                                                           
cACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGtt                                                            <  1:2933039/150‑1 (MQ=32)
cACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGtt                                                            <  1:3062390/150‑1 (MQ=32)
 aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTc                                                           <  2:3840775/150‑1 (MQ=32)
 aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTc                                                           <  2:3337212/150‑1 (MQ=32)
   ctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑a                                                        <  2:2287944/150‑1 (MQ=32)
      cttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑AAACATGGGTTCC‑AAT‑c                                                    >  1:1762105/1‑150 (MQ=12)
       ttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCT‑AAT‑cc                                                   >  1:4375811/1‑150 (MQ=17)
       ttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAAGGGGTCG‑AAT‑cc                                                   >  1:3828384/1‑150 (MQ=17)
        taccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑ccc                                                  >  1:3643895/1‑150 (MQ=17)
        taccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACACGGGTTCC‑AAT‑tcc                                                  >  1:1175610/1‑150 (MQ=12)
        taccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAAGGGGTCGTAAT‑cc                                                   >  2:2047669/1‑150 (MQ=255)
         accttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTTAAAAGGGGTTCG‑accccc                                                   >  2:2115927/1‑145 (MQ=255)
          ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCt                                                <  2:3820344/150‑1 (MQ=32)
           cttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTa                                               <  2:905548/150‑1 (MQ=32)
           cttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCT‑AAT‑accccc                                               >  2:4225715/1‑148 (MQ=12)
            ttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCTAg                                              >  1:2076968/1‑150 (MQ=17)
             taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCTAgg                                             >  1:2582722/1‑150 (MQ=17)
             taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑GTAAAGAGGGGCG‑AAT‑ACCCTAgg                                             >  2:1089201/1‑150 (MQ=11)
              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑GAT‑CCCCTAggg                                            >  1:297965/1‑150 (MQ=17)
              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAAGGGGTCG‑AAT‑CCCCCAggg                                            >  2:453024/1‑150 (MQ=12)
               aagaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAgggg                                           <  1:408602/150‑1 (MQ=32)
                agaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAAAAAGGGTTCG‑AAT‑CCCCCACGGGa                                          >  1:2613981/1‑150 (MQ=255)
                 gaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTTG‑GAT‑CCCCCTgggggg                                         >  2:3178795/1‑148 (MQ=255)
                 gaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCTAGGGGAc                                         >  2:2601234/1‑150 (MQ=17)
                  aagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCCAGGGGGCg                                        >  2:1643613/1‑150 (MQ=12)
                  aagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAACAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACATGGGCTCC‑AAA‑CCCCCCGGGGGCg                                        >  2:2259588/1‑150 (MQ=11)
                   agCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑ATACAGGGGGTCG‑AAT‑CCCCCCGGGGACGc                                       >  1:956951/1‑150 (MQ=11)
                    gCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TCAAAGGGGGTCG‑AAT‑CCCCTAcggggagcc                                      >  2:1950772/1‑145 (MQ=11)
                    gCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTTG‑AAT‑CCCCTAGGGGACGcc                                      >  2:1309847/1‑150 (MQ=17)
                     cGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCa                                     <  2:4392718/150‑1 (MQ=32)
                      gTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCTAGGGGACGCCAc                                    >  1:1187272/1‑150 (MQ=17)
                       ttCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACt                                   <  1:1909883/150‑1 (MQ=32)
                       ttCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCTAGGGGACGCCCCt                                   >  1:694692/1‑150 (MQ=17)
                        tCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACtt                                  <  2:3122356/150‑1 (MQ=32)
                        tCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCTAGGGGACGCCCCtt                                  >  1:2384829/1‑150 (MQ=17)
                        tCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAA‑CCCCTCGGGGACGCCACtt                                  >  2:2735958/1‑150 (MQ=12)
                         cTTTGCAGTGCTCACACAGATTGTCTGTTAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TTACAGAGGGTCG‑TAA‑CCCCTTGGGGCCGCCCCttc                                 >  2:601540/1‑149 (MQ=11)
                         cTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAA‑CCCCTAGGGGACGCCACCTg                                 >  1:1092712/1‑150 (MQ=12)
                          tttGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCCAGGGGGCGCCCCTTGc                                >  1:665586/1‑150 (MQ=255)
                            tGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAAGGGGTCG‑AAT‑CCCCTCGGGGGCGCCACctgctg                              >  2:2405217/1‑150 (MQ=11)
                            tGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑GAACAGGGGGTGG‑AAT‑CCCCTCGGGGGCGCCCCTTTCTg                              >  2:2850205/1‑150 (MQ=11)
                             gCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAAGGGGTCG‑AAT‑CCCCTAGGGGACGCCCCTTGCTgg                             >  2:947024/1‑150 (MQ=12)
                               aGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAAAAGGGGGTCG‑AAT‑CCCCCAGGGGACGCCACTTTCTGGtt                           >  2:3688669/1‑150 (MQ=255)
                               aGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAAAAAGGGGGCG‑AAT‑CCCCCAAGGGGGGCCACTTGCTgggt                           >  2:2257896/1‑148 (MQ=11)
                                gTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGttt                          <  1:699934/150‑1 (MQ=32)
                                  gCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTtgt                        <  1:3454236/150‑1 (MQ=32)
                                  gCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGGTtgt                        >  1:149421/1‑150 (MQ=17)
                                  gCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAA‑CCCCCAGGGGGCGCCCCCTGCTGGgtttt                        >  2:2391890/1‑148 (MQ=11)
                                   cTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCCAGGGGGCGCCACTTGCTGGGTtgtg                       >  2:3456931/1‑150 (MQ=255)
                                   cTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑AAAAAACGGGGCG‑GATACCCCCAGGGG‑CGCCCCCTGCTGGTTtgtg                       >  2:2421852/1‑150 (MQ=11)
                                    tCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTgtga                      <  1:1496847/150‑1 (MQ=32)
                                     cacacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTgtgag                     <  2:412706/150‑1 (MQ=32)
                                      acacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTgtgagt                    <  2:20894/150‑1 (MQ=32)
                                      acacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑CAACAGGGGGTTG‑AAT‑CCCCTAGGGGGCGCCACTTGCTGGTTTgtgagg                    >  1:128297/1‑149 (MQ=255)
                                       cacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTgtgagtg                   <  1:1709633/150‑1 (MQ=32)
                                       cacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAAGGGGTTG‑AAT‑CCCCTCGGGGACGCCACTTGCTGGTTTgtgagtg                   >  1:3225750/1‑150 (MQ=255)
                                       cacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑GAACAAGGGTTCG‑AAT‑CCCCCAGGGGGCGCCACTTGCTGGGTtgtgcatg                   >  1:2863328/1‑146 (MQ=11)
                                        acaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTgtgagtga                  <  1:3927227/150‑1 (MQ=32)
                                        acaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTgtgagtga                  <  1:4599264/150‑1 (MQ=32)
                                        acaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTgtgagtga                  >  1:785227/1‑150 (MQ=17)
                                        acaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAAGGGGTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTgtgagtga                  >  1:3259770/1‑150 (MQ=17)
                                        acaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAAAAGGGGGTCG‑AAT‑CCCCTAGGGGGCGCCACTTGCTGGGTTgtgagtga                  >  1:1697175/1‑150 (MQ=255)
                                         caGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGaa                 <  1:2301510/150‑1 (MQ=32)
                                         caGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTTTGAGTGaa                 >  1:1524262/1‑150 (MQ=17)
                                          aGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCTCGGGGGCGCCCCCTTCTGGTTTGTGAGTGaaa                >  2:1993712/1‑150 (MQ=11)
                                          aGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAAGGGGTCG‑AAT‑CCCCCAGGGGGCGCCACTTTCTGGTTTTTGAGTGaaa                >  1:4520790/1‑150 (MQ=11)
                                           gATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAg               <  1:2399548/150‑1 (MQ=32)
                                           gATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAg               <  1:2916285/150‑1 (MQ=32)
                                            aTTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGt              <  1:3171917/150‑1 (MQ=32)
                                            aTTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGt              <  2:1652263/150‑1 (MQ=32)
                                            aTTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑GAACACGGGGTCG‑AAT‑ACCCTATGGGGCCCCACTTGCTTGATTGTGAGGGCAAGt              >  1:1183206/1‑150 (MQ=11)
                                             ttGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TGACAAGGGTTCG‑AAT‑CCCCCAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTc             >  1:1627097/1‑150 (MQ=12)
                                             ttGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTc             <  1:722932/150‑1 (MQ=32)
                                               gTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCCAGGGGGAGCCCCTTGCTGGGTTGTGAGGGGAAGacac           >  1:1301954/1‑150 (MQ=11)
                                                tCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCAcc          <  2:748161/150‑1 (MQ=32)
                                                tCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCTAGGGGGCGCCACTTGCTGGTTTGTGAGTGGAAGTCAcc          >  1:2628484/1‑150 (MQ=12)
                                                tCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAAGGGGTCG‑AAT‑CCCCCCGGGGACGCCCCTTGCTTGTTTGTGAGGGGAAGTCAcc          >  2:1814603/1‑150 (MQ=11)
                                                tCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAAAAGGGGGTCG‑AAT‑CCCCCCGGGGGAGCCCCCTGCTGGTTTGTGAGTGAAAGTCAcc          >  2:658714/1‑150 (MQ=11)
                                                 cTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCt         <  1:1954791/150‑1 (MQ=32)
                                                 cTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCt         <  2:1113216/150‑1 (MQ=32)
                                                 cTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCt         <  2:3735550/150‑1 (MQ=32)
                                                 cTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCt         <  2:3867795/150‑1 (MQ=32)
                                                 cTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCt         <  1:1228512/150‑1 (MQ=32)
                                                 cTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCTAGGGGGCGCCACCTGCTGGGTTGTGAGTGGAAGTCACCt         >  2:3787742/1‑150 (MQ=11)
                                                 cTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCt         >  1:351954/1‑150 (MQ=17)
                                                 cTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCCCGGGGGCGCCCCCTGCTGGTTTGTGAGTGAAAAACACCt         >  2:530406/1‑150 (MQ=11)
                                                   gATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGc       <  2:2143197/150‑1 (MQ=32)
                                                   gATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGc       <  1:4305579/150‑1 (MQ=32)
                                                   gATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCTTGGGGGCGCCACCTGCTGGTTTTTTAGTGGAAGTCACCTGc       >  1:4533803/1‑150 (MQ=11)
                                                   gATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCCAGGGGACGCCCCTTGCTGGTTTGTGAGTGAAAGGCACCTGc       >  2:2593447/1‑150 (MQ=255)
                                                   gATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAAAAGGGGGTCG‑AAA‑CCCCCAGGGGGCGCCCCTTTCTGGTTTGTGGGGGAAAAGCACCCGc       >  2:3326909/1‑150 (MQ=11)
                                                     tAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑GAACAGGGGGTCG‑AAT‑CCCCCAGGGGGCGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCt     >  2:1381861/1‑150 (MQ=255)
                                                      aGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCtt    <  2:1357530/150‑1 (MQ=32)
                                                      aGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCtt    <  1:2520379/150‑1 (MQ=32)
                                                      aGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCtt    <  2:972141/150‑1 (MQ=32)
                                                       ggAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCTCGGGGGCGCCCCTTGCTGGTTTGTGAGTGAAAGTCACCTGCCttt   >  2:48489/1‑149 (MQ=255)
                                                        gAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGG‑AAAAAGGGGTTCG‑‑AT‑CCCCTAGGGGGGGCCACTTTCTGGGTTGTGGGTGAAAGACACCTGCCTTaa  >  2:309751/1‑150 (MQ=11)
                                                        gAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTaa  <  1:3296935/150‑1 (MQ=11)
                                                        gAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTaa  >  2:526596/1‑150 (MQ=11)
                                                        gAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGGTCG‑AAT‑CCCCCAGGGGGCGCCCCTTGCTGGTTTGTGAGTGAAAGACACCTGCCttta  >  2:4063724/1‑148 (MQ=11)
                                                                                                                                                      |                                                           
CACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GG‑TAACAGGGGTTCG‑AAT‑CCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAA  >  AP012306/3372553‑3372758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑