Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,590,3670AC22.6% 827.9 / 37.1 301T30P (ACC→CCC) fabRDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (221/12);  new base C (0/68);  total (221/80)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 325 total aligned reads displayed.

AGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTG  >  AP012306/3590218‑3590516
                                                                                                                                                      |                                                                                                                                                     
 gAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAAc                                                                                                                                                      >  2:1029261/1‑150 (MQ=255)
 gAACTGCATTAATTATGTTTTCTTGCGTTAGCGTTAATTCACAATGTTGGTGCAATCCAGCATGGTCATTCTCTGGATTGGTACCAGCAGCACATCGGGCAC‑GGTTTCGAACGTCGGTGTGGGCGTAAGAGCGCAACAATAAGAAAacac                                                                                                                                                      >  2:1757026/1‑150 (MQ=255)
  aaTTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAAcc                                                                                                                                                     <  1:854576/150‑1 (MQ=255)
    ttGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACccg                                                                                                                                                   >  2:2755395/1‑150 (MQ=255)
     tGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACccgc                                                                                                                                                  >  1:601770/1‑150 (MQ=255)
      gCAGTAAATTTGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACccgcc                                                                                                                                                 <  2:1984504/150‑1 (MQ=255)
       caatAAATATGTTTTTTTGTTTTCCCGTTCATTCACAATTCTGGAGCAACCCAGTATGTTCATTCTCGGGTAAAGGGCAAGCAGTACTTTTGAAAG‑GGATTCAGACATCGTGATGGGCGAAAGAGCGCAAAAAAAAAAAAAACCccgccg                                                                                                                                                <  2:76011/147‑1 (MQ=255)
       cAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACccgccg                                                                                                                                                >  1:3899426/1‑150 (MQ=255)
       cAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAATATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACccgccg                                                                                                                                                >  1:1994325/1‑150 (MQ=255)
        aGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGt                                                                                                                                               >  2:126880/1‑150 (MQ=255)
         gTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGtt                                                                                                                                              >  2:146257/1‑150 (MQ=255)
          tAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTc                                                                                                                                             >  2:2313721/1‑150 (MQ=255)
           aaaTATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCg                                                                                                                                            >  2:1866935/1‑150 (MQ=255)
           aaaTATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCg                                                                                                                                            >  2:3275575/1‑150 (MQ=255)
            aaTATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGc                                                                                                                                           >  1:3643961/1‑150 (MQ=255)
             atttGTTTTATGGCTTTCCCTTTCATTCACAATACTGGAGCAACCCAGTATTTTCTTCCTCTGGTATGGGCCCAGCAGTACTTGGGGCAA‑GGATTCAGACATCGTGGGGGGCGTAAGCGCCCAACAAAAAAAAAAACCCCGCCGTTCGCt                                                                                                                                          <  2:373118/147‑1 (MQ=255)
             atatGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCt                                                                                                                                          >  2:3387703/1‑150 (MQ=255)
              tatTTTTTTTTGCGTTCCCTTTCATTCAAAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGAAA‑GGATTCAGCCATGGTGAGGGGCGAAAGAGCAAAAAAAAAAAAAAAACCCCGCCGTTCGCtg                                                                                                                                         <  2:3942254/150‑1 (MQ=255)
                tGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCtggt                                                                                                                                       >  1:230293/1‑150 (MQ=255)
                 gTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCtggtg                                                                                                                                      >  1:112160/1‑150 (MQ=255)
                 gTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCtggtg                                                                                                                                      >  2:2429571/1‑150 (MQ=255)
                 gTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCtggtg                                                                                                                                      >  2:1258452/1‑150 (MQ=255)
                  ttttATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATCGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCtggtgg                                                                                                                                     >  1:2818184/1‑150 (MQ=255)
                   tttATTGCTTTACCGTTCATTCACAATACGGGAGCAATCCAGTATGTTCATTCTCGGGTATAGTGCCAGCATTACTTTTGGCAA‑GAATTCAGACATCGTGAGGGGGGAAAGAGCAAAAAAAAAAAAAAAAACCCGCCGTTCGCTGGTGGa                                                                                                                                    <  2:309468/150‑1 (MQ=255)
                    ttATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGaa                                                                                                                                   >  1:2973491/1‑150 (MQ=255)
                     tATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAg                                                                                                                                  >  1:2426622/1‑150 (MQ=255)
                     tATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAg                                                                                                                                  >  1:4106067/1‑150 (MQ=255)
                      aTTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGc                                                                                                                                 >  2:4434588/1‑150 (MQ=255)
                         gCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGc                                                                                                                              >  2:4501126/1‑150 (MQ=255)
                         gCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGc                                                                                                                              >  2:3813576/1‑150 (MQ=255)
                         gCGTTACCGTTCATTCACAATACTGGAGCAACCCAGTATGTTCATTCTCTGGTATAGGGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGc                                                                                                                              >  2:1562853/1‑150 (MQ=255)
                              aCCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTa                                                                                                                         >  1:1716571/1‑150 (MQ=255)
                                cGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGc                                                                                                                       >  1:1459132/1‑150 (MQ=255)
                                cGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGc                                                                                                                       >  1:834602/1‑150 (MQ=255)
                                 gTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGcc                                                                                                                      >  1:1471890/1‑150 (MQ=255)
                                  ttcattcaCAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCa                                                                                                                     >  2:4157286/1‑150 (MQ=255)
                                   tcattcaCAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCaa                                                                                                                    >  1:3961857/1‑150 (MQ=255)
                                      ttcaCAATACGGGACCAACCCAGTTTGTTCATTCTCTGGTATAGCGCCAGCAGTCTTTTGGGAAA‑GGATCCAGCCCCCGGGAGGGGCGAAAGAGCGAAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTa                                                                                                                 <  2:1203946/150‑1 (MQ=255)
                                       tcaCAATCCTGGAGCAATCCAGTATTTTCATTCTCTGGTATAGTGCAAGCAGTACTTTTGGAAG‑GGATTCAGACATCGGGAGGGGCGAAAGAGCGAAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTaa                                                                                                                <  2:2639575/150‑1 (MQ=255)
                                       tcaCAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTaa                                                                                                                >  1:1746447/1‑150 (MQ=255)
                                         acaATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGAAA‑GGATTCAGACATCGTGAGGGGCGAAAGAGCAAAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGt                                                                                                              <  2:3675858/150‑1 (MQ=255)
                                          caATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTg                                                                                                             >  2:2409465/1‑150 (MQ=255)
                                           aaTACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGc                                                                                                            >  1:2726370/1‑150 (MQ=255)
                                           aaTACTGGAGAAATCCAGTATGTTCATTCTGTGTTAAAGTGCCAGCAGTATTTTGGGAAA‑GAATTCAGACATGGTGGGGGGGGAAAGAGCAAAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGc                                                                                                            <  2:4281266/150‑1 (MQ=255)
                                             tACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTg                                                                                                          >  1:2055080/1‑150 (MQ=255)
                                                tGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAAc                                                                                                       >  2:2913466/1‑150 (MQ=255)
                                                   aGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCa                                                                                                    >  2:3587513/1‑150 (MQ=255)
                                                      aaTCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCt                                                                                                 >  1:96567/1‑150 (MQ=255)
                                                           aGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGcc                                                                                            >  2:1481116/1‑150 (MQ=255)
                                                            gTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCa                                                                                           >  2:3598549/1‑150 (MQ=255)
                                                                tttctttctGTGGTATAGTCCCAGCATTACTTTGGGCAA‑GGATTCAACCTGGGTGAGGGGGGAAAGACCGAAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCt                                                                                       <  2:1901047/149‑1 (MQ=255)
                                                                 ttCATTCTCTGGTATAGTGCCAGCAGTTCTTTGGGAAA‑GGATTCAGCCATCGTGAGGGGCGAAAGCGCAAAAAAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTg                                                                                      <  2:2701291/150‑1 (MQ=255)
                                                                    aTTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGt                                                                                   >  1:1951149/1‑150 (MQ=255)
                                                                    aTTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGt                                                                                   >  1:3377112/1‑150 (MQ=255)
                                                                      tctcTGGTATAGTGCCAGCAGAACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGttt                                                                                 >  1:3857816/1‑150 (MQ=255)
                                                                            gTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGCGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGCGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGa                                                                           >  1:2506258/1‑150 (MQ=255)
                                                                             tataGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGaa                                                                          >  2:3194315/1‑150 (MQ=255)
                                                                             tataGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGAGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGCTTGCGTGaa                                                                          >  2:285604/1‑150 (MQ=255)
                                                                              ataGTGCCAGCAGTATTTTTGGCAA‑GGATCCAGACATGGTGATGGGCGAAAGAGCGCAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAg                                                                         <  2:1706662/150‑1 (MQ=255)
                                                                               taGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGt                                                                        >  1:4292061/1‑150 (MQ=255)
                                                                                 gTGCCGGAAGTATTTTTGGAAA‑GGATTCAGACATCGTGAGGGGCGAAAGAGCGAAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTgg                                                                      <  1:3503960/150‑1 (MQ=255)
                                                                                 gTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTgg                                                                      >  1:528191/1‑150 (MQ=255)
                                                                                       gcacTATTTTAGGAAG‑GGATTCACACTGGGGGAGGGGTGAAAGAGCACAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTg                                                                <  2:861881/150‑1 (MQ=255)
                                                                                        cagTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGa                                                               >  2:2788306/1‑150 (MQ=255)
                                                                                         agTATTTTGGGAAA‑GGATTAAACCATGGTGAGGGGGGAAAGAGCAAAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGaa                                                              <  2:92738/150‑1 (MQ=255)
                                                                                         agTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGaa                                                              >  1:1650789/1‑150 (MQ=255)
                                                                                          agttctttGGCAAGGGATCGAGACGGCGTGATGGGTGAAAGAGCCAAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGaa                                                              <  1:3221135/145‑1 (MQ=255)
                                                                                            aCTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCg                                                           >  2:1337620/1‑150 (MQ=255)
                                                                                               tttGGGAAGGGATTCAGACAGGGGGAGGGGTGAAAGAGCGCAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCggg                                                         <  1:2903432/150‑1 (MQ=255)
                                                                                                ttGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCa                                                       >  1:2487937/1‑150 (MQ=255)
                                                                                                  gaaaa‑GGATTCAGACATCGGGGGGGGCGAAAGCGCAAAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCAtt                                                     <  2:2144493/147‑1 (MQ=255)
                                                                                                    cAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGc                                                   >  2:4244442/1‑150 (MQ=255)
                                                                                                     aa‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCt                                                  >  2:1658847/1‑150 (MQ=255)
                                                                                                         gATTCAGACATCGTGAGGGGCGAAAGAGCGAAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTccc                                               <  1:3735412/150‑1 (MQ=255)
                                                                                                                aCATCGTGAGGGGCGAAAGAGCGCAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCtt                                        <  1:1830753/150‑1 (MQ=255)
                                                                                                                    cGTGATGGGCGAAAGAGCGAAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTa                                    <  1:2670773/150‑1 (MQ=255)
                                                                                                                     ggggtGGGCGAAGGAGCGAAACAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTAt                                   <  2:612400/146‑1 (MQ=255)
                                                                                                                         ggggCGAAAGAGCAAAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGc                               <  1:1009683/149‑1 (MQ=255)
                                                                                                                            gCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCAtt                            >  2:386760/1‑150 (MQ=255)
                                                                                                                            gCGTAAGAGCACAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCAtt                            <  1:3020073/150‑1 (MQ=255)
                                                                                                                            gCGAAAGAGCAAAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCAtt                            <  2:3459422/150‑1 (MQ=255)
                                                                                                                             cgaaaGAGCAAAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCAttt                           <  1:3444699/147‑1 (MQ=255)
                                                                                                                               tAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTcc                         >  2:1829806/1‑150 (MQ=255)
                                                                                                                               tAAGAGAGAAAAAAAAAAAAAACACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCTTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTcc                         <  2:3049280/150‑1 (MQ=255)
                                                                                                                                aaGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCcg                        >  2:2557270/1‑150 (MQ=255)
                                                                                                                                aaGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCcg                        >  2:1396977/1‑150 (MQ=255)
                                                                                                                                 ggagCACAAAAAAAAAAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCcgc                       <  1:548586/149‑1 (MQ=255)
                                                                                                                                      gcAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGt                  >  2:2079394/1‑150 (MQ=255)
                                                                                                                                      gcAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGt                  >  2:2972511/1‑150 (MQ=255)
                                                                                                                                          aaaaaaaaaaaACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGAc              <  1:472219/149‑1 (MQ=255)
                                                                                                                                           aaaaaGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACg             >  1:4167371/1‑150 (MQ=255)
                                                                                                                                            aaaaGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGa            >  1:4537697/1‑150 (MQ=255)
                                                                                                                                              aaGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAAc          >  1:3288046/1‑150 (MQ=255)
                                                                                                                                                gAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTg        >  2:3412349/1‑150 (MQ=255)
                                                                                                                                                aaaaaacCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTg        <  1:3792999/149‑1 (MQ=255)
                                                                                                                                                 aaaaaaCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACCAACTgg       >  1:3108533/1‑150 (MQ=255)
                                                                                                                                                  aaaaaCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTggg      >  1:4401185/1‑150 (MQ=255)
                                                                                                                                                     aaCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCt   >  1:1967946/1‑150 (MQ=255)
                                                                                                                                                      aCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTg  >  1:744673/1‑150 (MQ=255)
                                                                                                                                                      aCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTg  >  1:258953/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
AGAATTGCAGTAAATATGTTTTATTGCGTTACCGTTCATTCACAATACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAA‑GGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTG  >  AP012306/3590218‑3590516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑