Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,639,0400TG25.6% 179.4 / 15.2 82noncoding (54/76 nt)gltVtRNA‑Glu
Reads supporting (aligned to +/- strand):  ref base T (1/60);  new base G (21/0);  total (22/60)
Fisher's exact test for biased strand distribution p-value = 1.20e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 143 total aligned reads displayed.

CACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑GAAAGTCACCTGCCTTAA  >  AP012306/3638892‑3639097
                                                                                                                                                     |                                                          
cACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑tt                                                           <  1:90131/150‑1 (MQ=32)
 aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTc                                                          <  2:1065998/150‑1 (MQ=32)
  cctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCg                                                         >  1:702718/1‑150 (MQ=17)
  cctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCg                                                         >  1:3114274/1‑150 (MQ=17)
   ctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGa                                                        >  1:1875222/1‑150 (MQ=17)
   ctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGAAACAAGGG‑GTcca                                                        >  1:402680/1‑148 (MQ=12)
     ccttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAAt                                                      <  1:4330018/150‑1 (MQ=32)
     ccttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAAt                                                      >  1:1631373/1‑150 (MQ=32)
      cttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATc                                                     <  2:3703426/150‑1 (MQ=32)
       ttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGG‑GTCGAATcc                                                    >  1:4266590/1‑150 (MQ=17)
        taccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAAAAAGGG‑GTCGAAAccc                                                   >  2:3524894/1‑150 (MQ=255)
         accttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATcccc                                                  <  1:4609876/150‑1 (MQ=32)
          ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCt                                                 <  1:1078470/150‑1 (MQ=32)
           cttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTTTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTa                                                <  2:1960141/150‑1 (MQ=17)
           cttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTa                                                <  1:1039418/150‑1 (MQ=32)
           cttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTTGAAAccccca                                                >  2:2409308/1‑148 (MQ=255)
           cttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATccccca                                                >  2:2351049/1‑148 (MQ=17)
           cttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAAAAAGGG‑TTTGAATCCCCtt                                                >  1:4525185/1‑149 (MQ=12)
            ttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCTAg                                               >  1:231097/1‑150 (MQ=17)
            ttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGG‑GTCGAATCCCCTAg                                               >  1:3664488/1‑150 (MQ=17)
             taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAgg                                              <  1:1828341/150‑1 (MQ=32)
             taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAgg                                              <  1:1320641/150‑1 (MQ=32)
             taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGG‑GTCGAATCCCCCAgg                                              >  2:3770431/1‑150 (MQ=12)
             taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGGCACAGGGG‑GTCGAAACCCCCAgg                                              >  2:2959714/1‑150 (MQ=11)
              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCTAggg                                             >  1:1492888/1‑150 (MQ=17)
              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCTAggg                                             >  1:350658/1‑150 (MQ=17)
              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCCAggg                                             >  1:4301203/1‑150 (MQ=17)
              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGGAACAGGGG‑GTCGAATtcccccggg                                             >  1:2723947/1‑145 (MQ=255)
               aagaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAgggg                                            <  1:1199270/150‑1 (MQ=32)
               aagaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCTAgggg                                            >  1:2703530/1‑150 (MQ=17)
                agaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGGGAAAAGCAAGCCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGCCTCGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTTCCAATccctacgggg                                            >  2:3823844/1‑143 (MQ=11)
                 gaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGAc                                          <  1:1065033/150‑1 (MQ=32)
                 gaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCCAgggggc                                          >  2:2682719/1‑148 (MQ=17)
                  aagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACg                                         <  1:1275726/150‑1 (MQ=32)
                  aagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGTATCCCCCTGGGGGCg                                         >  1:2646324/1‑150 (MQ=11)
                  aagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCTCGGGGGCg                                         >  2:4383706/1‑150 (MQ=12)
                  aagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCTAGGGGGCg                                         >  1:2040068/1‑150 (MQ=17)
                   agCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGG‑GGCGAATTCCCTCGGGGGCgg                                        >  2:2085271/1‑149 (MQ=11)
                    gCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGcc                                       >  1:3465032/1‑150 (MQ=32)
                    gCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGcc                                       <  2:770148/150‑1 (MQ=32)
                    gCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCTAGGGGACGcc                                       >  1:852813/1‑150 (MQ=17)
                      gTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATTCCCTAGGGGGCGCCAc                                     >  1:790240/1‑150 (MQ=12)
                      gTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGG‑GTCGAATCCCCCAGGGGACGcccc                                     >  2:3304944/1‑148 (MQ=255)
                       ttCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCCAGGGGGCGCCACt                                    >  2:3893046/1‑150 (MQ=12)
                       ttCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCCAAGGGACGCCACt                                    >  1:2518465/1‑150 (MQ=12)
                       ttCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGGAACAAGGG‑GTCGAATCCCCCAGGGGGCGCCACt                                    >  1:1551393/1‑150 (MQ=11)
                          tttGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGTAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGc                                 <  1:4307562/150‑1 (MQ=17)
                          tttGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGc                                 <  2:2834751/150‑1 (MQ=32)
                          tttGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCCAGGGGGCGCCCCCTGc                                 >  2:2660041/1‑150 (MQ=11)
                          tttGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGG‑GTCGAATCCCCCAGGGGGCGCCCCTTGc                                 >  2:596369/1‑150 (MQ=11)
                          tttGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAAACCGGG‑GTCGAAACCCCCAGGGGACGCCCCCTGc                                 >  2:228501/1‑150 (MQ=11)
                            tGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTg                               <  1:757079/150‑1 (MQ=32)
                            tGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGG‑GTCGAATCCCCCAGGGGGCGCCCCTTGCTg                               >  2:1556017/1‑150 (MQ=11)
                             gCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCCAGGGGCCGCCCCCTGCttg                              >  2:1638795/1‑148 (MQ=11)
                              cAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCTAGGGGGCGCCACCTGCTGGt                             >  1:4271608/1‑150 (MQ=12)
                               aGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCTAGGGGGCGCCACTTGCTGGtt                            >  1:4233749/1‑150 (MQ=17)
                               aGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCTAGGGGACGCCACTTGCTGGtt                            >  1:2482698/1‑150 (MQ=17)
                               aGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGG‑TTCGAATCCCCTAGGGGGCGCCACTTGCTGGtt                            >  2:2422969/1‑150 (MQ=17)
                                gTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGttt                           <  1:311759/150‑1 (MQ=32)
                                  gCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtgt                         <  2:2917544/150‑1 (MQ=32)
                                   cTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtgtg                        <  1:1229724/150‑1 (MQ=32)
                                   cTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCTAGGGGGCGCCACTTGCTGGTTtttg                        >  1:3262494/1‑150 (MQ=12)
                                    tCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTTACAAGGG‑GTCGGATCCCCTAGGGGGCGCCACTTGCTGGGTtgtgg                       >  1:2172018/1‑149 (MQ=11)
                                    tCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtga                       <  1:6612/150‑1 (MQ=32)
                                      acacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAAAAGGGG‑TTCGAAACCCCCAGGGGAAGGCACTTGCTGGTTtgtggga                     >  2:4139178/1‑147 (MQ=11)
                                       cacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgagt‑g                   <  2:800137/150‑1 (MQ=32)
                                       cacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAAACCCCCAGGGGACGCCACTTGCTGGGTTgtgagt‑g                   >  1:4064861/1‑150 (MQ=12)
                                         caGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCTAGGGGACGCCCCTTGCTGGTTTGTGAGG‑Gaa                 >  1:3552724/1‑150 (MQ=12)
                                         caGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCCCGGGGGCGCCACCTGCTGGGTtgtgggggga                  >  2:3754258/1‑144 (MQ=11)
                                         caGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGG‑GTCGAATCCCCTAGGGGACGCCACTTGCTGGGTTGTGAGT‑Gaa                 >  1:3343510/1‑150 (MQ=12)
                                         caGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGGAACAGGGG‑GTCGAAACCCCCAGGGGACGCCACTTGCTGGGTTttgagggga                  >  1:4486888/1‑146 (MQ=11)
                                          aGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑Gaaa                <  2:4564514/150‑1 (MQ=32)
                                          aGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑Gaaa                <  2:3165863/150‑1 (MQ=32)
                                          aGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGGAACAAGGG‑GTCGAATCCCCCAGGGGGCGCCCCCTTCTTGGTTGTGGGT‑Ggaa                >  2:1046728/1‑150 (MQ=11)
                                           gATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCTAGGGGGCGCCACTTGCTGGTTTGTGAGT‑GAAAg               >  1:1621230/1‑150 (MQ=17)
                                            aTTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑GAAAGt              <  2:3521732/150‑1 (MQ=32)
                                            aTTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGG‑TTCGGATACCCCAGGGGGCGCCCCATTCTGGTTTGTGAGT‑GGAAGt              >  2:4187611/1‑150 (MQ=11)
                                            aTTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAAAACGGG‑GTCGAATCCCCCAGGGGGCGCCCCCTTCCGGTTTGTGGGT‑Gaaaag              >  2:382487/1‑148 (MQ=11)
                                             ttGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑GAAAGTc             <  2:1097654/150‑1 (MQ=32)
                                             ttGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑GAAAGTc             <  2:350046/150‑1 (MQ=32)
                                               gTCTGATAGAAATTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑GAAAGTCAc           <  2:2873838/150‑1 (MQ=17)
                                               gTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑GAAAGTCAc           <  1:4024286/150‑1 (MQ=32)
                                               gTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑GAAAGTCAc           <  2:4076824/150‑1 (MQ=32)
                                               gTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑GAAAGTCAc           <  2:3416286/150‑1 (MQ=32)
                                               gTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCCAGGGGACGCCACTTGCTGGTTTGTGAGTGGAAAGtac            >  1:3720388/1‑148 (MQ=255)
                                               gTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGG‑GTCGAATCCCCTCGGGGGCGCCCCTTGCTGGTTTTTGAGT‑Gaaaagcac           >  2:3990880/1‑145 (MQ=11)
                                                tCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑GAAAGTCAcc          <  1:2329192/150‑1 (MQ=32)
                                                 cTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTCCCAAGGG‑TGCTCACCCCCCACGGGGGGCCCCTTACTGGTTTTTGGGT‑GAAAgagaacc         >  2:1044831/1‑144 (MQ=11)
                                                 cTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑GAAAGTCACCt         <  2:3026707/150‑1 (MQ=32)
                                                 cTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑GAAAGTCACCt         <  1:638274/150‑1 (MQ=32)
                                                 cTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGG‑TTCGAATCCCCCAGGGGACGCCCCCTGCTGGGTTGTGAGT‑GAAAGGCACCt         >  2:4456789/1‑150 (MQ=11)
                                                    aTAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCTAGGGGGCGCCACTTGCTGGGTTTTGAGT‑GGAAGTCACCTGcc      >  1:493326/1‑150 (MQ=11)
                                                     tAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑GAAAGTCACCTGCCt     <  1:420985/150‑1 (MQ=32)
                                                     tAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑GAAAGTCACCTGCCt     <  1:2065859/150‑1 (MQ=32)
                                                      aGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑GAAAGTCACCTGCCtt    <  2:3345790/150‑1 (MQ=32)
                                                      aGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑GAAAGTCACCTGCCtt    <  1:2840373/150‑1 (MQ=32)
                                                      aGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑GAAAGTCACCTGCCtt    <  1:1460676/150‑1 (MQ=32)
                                                      aGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGAATCCCCCAGGGGGCGCCCCTTGCTGGGTTGTGAGG‑GGAAGGCACCTGCCtt    >  2:3218314/1‑150 (MQ=11)
                                                        aaaGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑GTCGGATCCCCTAGGGGGCGCCACTTGCTGGTTTGTGAGT‑GAAAGTCACCTGCCTTaa  >  1:297971/1‑150 (MQ=2)
                                                        aaaGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAAGGG‑GTTGAATACCCTAAGGGGCGCCCCCTTCTGGTTTTTGGGT‑GGAAATCACCCGCCCTaa  >  2:3332213/1‑150 (MQ=11)
                                                                                                                                                     |                                                          
CACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGG‑TTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGT‑GAAAGTCACCTGCCTTAA  >  AP012306/3638892‑3639097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑