Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 3,162,768 A→T D5E (GAT→GAA)  glyQ ← glycine tRNA synthetase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,162,7680AT100.0% 1210.4 / NA 300D5E (GAT→GAA) glyQglycine tRNA synthetase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (142/158);  total (142/158)

Only 100 of 304 total aligned reads displayed.

CATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGT  >  AP012306/3162621‑3162914
                                                                                                                                                   |                                                                                                                                                  
cATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTc                                                                                                                                                  <  2:550759/150‑1 (MQ=255)
    ggTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAAc                                                                                                                                              >  2:1384543/1‑150 (MQ=255)
     gTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACt                                                                                                                                             <  1:376734/150‑1 (MQ=255)
      ttCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACtt                                                                                                                                            <  2:885628/150‑1 (MQ=255)
      ttCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACtt                                                                                                                                            >  2:3315349/1‑150 (MQ=255)
         tGGCCCCAGCTCGCGCAGACAGGTCATTGGGCGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTg                                                                                                                                         >  2:4177407/1‑150 (MQ=255)
            ccccAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCat                                                                                                                                      <  1:576788/150‑1 (MQ=255)
            ccccAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCat                                                                                                                                      <  2:604260/150‑1 (MQ=255)
             cccAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCata                                                                                                                                     <  1:711013/150‑1 (MQ=255)
              ccAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCatat                                                                                                                                    <  1:3589525/150‑1 (MQ=255)
               cAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCAtatt                                                                                                                                   <  2:1560262/150‑1 (MQ=255)
                aGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCAtatta                                                                                                                                  >  2:187482/1‑150 (MQ=255)
                aGCTCGCGCAGACAGGACATTGGGTGAGAGGTTCCCGCGGCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCAtatta                                                                                                                                  <  2:2203021/150‑1 (MQ=255)
                  cTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATattatt                                                                                                                                <  1:2307998/150‑1 (MQ=255)
                    cgcgCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTc                                                                                                                              <  2:2689570/150‑1 (MQ=255)
                     gcgcAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCg                                                                                                                             <  1:1109381/150‑1 (MQ=255)
                        cAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGc                                                                                                                          <  1:50331/150‑1 (MQ=255)
                        cAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGc                                                                                                                          <  2:254069/150‑1 (MQ=255)
                           aCAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTgg                                                                                                                       <  1:4370/150‑1 (MQ=255)
                             aGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGAt                                                                                                                     <  1:673747/150‑1 (MQ=255)
                               gTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATAc                                                                                                                   <  1:4136513/150‑1 (MQ=255)
                                tCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACg                                                                                                                  <  2:4012964/150‑1 (MQ=255)
                                  aTTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACgtg                                                                                                                >  2:4368841/1‑150 (MQ=255)
                                      ggTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGa                                                                                                            >  2:1325081/1‑150 (MQ=255)
                                        tGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGAtt                                                                                                          <  1:3644944/150‑1 (MQ=255)
                                            agGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTaaa                                                                                                      >  2:3541585/1‑150 (MQ=255)
                                            agGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTaaa                                                                                                      <  1:2997203/150‑1 (MQ=255)
                                              gTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAaagg                                                                                                    <  2:1034318/150‑1 (MQ=255)
                                              gTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAaagg                                                                                                    <  2:2805498/150‑1 (MQ=255)
                                               ttCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAaagga                                                                                                   <  1:4602001/150‑1 (MQ=255)
                                                tCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAaaggaa                                                                                                  >  1:387155/1‑150 (MQ=255)
                                                tCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAaaggaa                                                                                                  >  2:2837418/1‑150 (MQ=255)
                                                  ccGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAaaggaagg                                                                                                <  2:2296814/150‑1 (MQ=255)
                                                   cgcgCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAggg                                                                                               <  1:912366/150‑1 (MQ=255)
                                                     cgcCGGCTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGAt                                                                                             <  2:1128576/150‑1 (MQ=255)
                                                        cGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAg                                                                                          <  1:95122/150‑1 (MQ=255)
                                                         gACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGt                                                                                         >  1:1289553/1‑150 (MQ=255)
                                                          aCTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGta                                                                                        >  2:1005582/1‑150 (MQ=255)
                                                          aCTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGta                                                                                        >  2:2142196/1‑150 (MQ=255)
                                                          aCTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGta                                                                                        <  1:3456378/150‑1 (MQ=255)
                                                          aCTTCCATGTCCAATGGTTGAACAATGGGGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGGTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGTAGGGATCAGta                                                                                        >  1:3260009/1‑150 (MQ=255)
                                                            ttCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGtata                                                                                      >  1:3503979/1‑150 (MQ=255)
                                                             tCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATAc                                                                                     >  1:2563817/1‑150 (MQ=255)
                                                              ccATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATAcc                                                                                    >  1:807934/1‑150 (MQ=255)
                                                               cATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATAccc                                                                                   <  1:1512981/150‑1 (MQ=255)
                                                                   tCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCtg                                                                               <  2:1487230/150‑1 (MQ=255)
                                                                    ccAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCtgg                                                                              >  2:3418113/1‑150 (MQ=255)
                                                                     cAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCtgga                                                                             <  1:1602107/150‑1 (MQ=255)
                                                                      aaTGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCtggat                                                                            <  2:4262177/150‑1 (MQ=255)
                                                                       aTGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCtggatg                                                                           <  2:695956/150‑1 (MQ=255)
                                                                        tGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCtggatgg                                                                          <  2:3175968/150‑1 (MQ=255)
                                                                        tGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCtggatgg                                                                          <  1:2441959/150‑1 (MQ=255)
                                                                         ggTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCtggatgga                                                                         >  2:1215434/1‑150 (MQ=255)
                                                                           ttGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAg                                                                       >  1:1071166/1‑150 (MQ=255)
                                                                                cAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGAtata                                                                  >  2:4572751/1‑150 (MQ=255)
                                                                                    ggTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGt                                                              <  2:3738062/150‑1 (MQ=255)
                                                                                    ggTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGt                                                              <  1:4515509/150‑1 (MQ=255)
                                                                                    ggTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGt                                                              <  2:2911951/150‑1 (MQ=255)
                                                                                          gCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGaaa                                                        <  1:553127/150‑1 (MQ=255)
                                                                                          gCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGaaa                                                        <  2:854574/150‑1 (MQ=255)
                                                                                          gCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGAGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGaaa                                                        >  1:4319416/1‑150 (MQ=255)
                                                                                           cccTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAAc                                                       >  1:3108297/1‑150 (MQ=255)
                                                                                            ccTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACg                                                      >  1:1002690/1‑150 (MQ=255)
                                                                                            ccTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACg                                                      >  2:4116275/1‑150 (MQ=255)
                                                                                              tGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACggg                                                    >  1:2867014/1‑150 (MQ=255)
                                                                                                  gAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGaaaa                                                >  2:246916/1‑150 (MQ=255)
                                                                                                  gAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGaaaa                                                >  1:383561/1‑150 (MQ=255)
                                                                                                   aGCCGAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGaaaaa                                               <  1:1780862/150‑1 (MQ=255)
                                                                                                     cccAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGc                                             <  1:3940939/150‑1 (MQ=255)
                                                                                                      ccAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCa                                            >  1:2016934/1‑150 (MQ=255)
                                                                                                       cAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAg                                           <  1:2213094/150‑1 (MQ=255)
                                                                                                        aGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAgg                                          <  2:1253855/150‑1 (MQ=255)
                                                                                                         gTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATTCCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAggg                                         <  2:4521665/150‑1 (MQ=255)
                                                                                                         gTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAggg                                         <  1:4050474/150‑1 (MQ=255)
                                                                                                            aTCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCtt                                      >  1:1414597/1‑150 (MQ=255)
                                                                                                            aTCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCtt                                      <  2:3570720/150‑1 (MQ=255)
                                                                                                             tCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTa                                     <  2:2052193/150‑1 (MQ=255)
                                                                                                             tCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTa                                     <  2:2979245/150‑1 (MQ=255)
                                                                                                              ccTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTaa                                    <  1:2905277/150‑1 (MQ=255)
                                                                                                                 gTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGc                                 >  1:1914894/1‑150 (MQ=255)
                                                                                                                   aaGGTCAGGGTCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCAt                               >  1:3360999/1‑150 (MQ=255)
                                                                                                                   aaGGTCAGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCAt                               <  2:4497965/150‑1 (MQ=255)
                                                                                                                        catgATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGaaa                          >  2:1978957/4‑150 (MQ=255)
                                                                                                                        cAGGATCAAGCCCTGGAAGGTCCTGGGTTCAAACTTTTGCATATTATTTCGTGCTGGATACGGGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGaaa                          >  2:1716351/1‑150 (MQ=255)
                                                                                                                         aGGATCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAg                         >  2:560493/1‑150 (MQ=255)
                                                                                                                            aTCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATg                      >  2:2429161/1‑150 (MQ=255)
                                                                                                                             tCAAGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGc                     >  2:397346/1‑150 (MQ=255)
                                                                                                                               aaGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCaa                   >  1:374370/1‑150 (MQ=255)
                                                                                                                               aaGCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCaa                   >  2:3769398/1‑150 (MQ=255)
                                                                                                                                 gCCCTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCaaaa                 <  1:4224109/150‑1 (MQ=255)
                                                                                                                                  cccTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCaaaaa                >  1:2187224/1‑150 (MQ=255)
                                                                                                                                  cccTGGAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCaaaaa                >  2:1671370/1‑150 (MQ=255)
                                                                                                                                      ggAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATgg            <  1:4576155/150‑1 (MQ=255)
                                                                                                                                       gAAGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGc           <  2:747690/150‑1 (MQ=255)
                                                                                                                                        aaGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGcc          >  2:3498083/1‑150 (MQ=255)
                                                                                                                                        aaGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGcc          >  2:754702/1‑150 (MQ=255)
                                                                                                                                         aGGTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCa         >  2:826071/1‑150 (MQ=255)
                                                                                                                                           gTCCTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATc       <  1:184110/150‑1 (MQ=255)
                                                                                                                                             ccTGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCcg     >  2:3457815/1‑150 (MQ=255)
                                                                                                                                               tGGTTTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCcgcg   <  2:3050656/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGT  >  AP012306/3162621‑3162914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑