New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 2743199377 (1.220)6 (0.030)
+CGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCAGATTGTTG		 4/186 17.0 2.7% intergenic (+264/+350) yhaC/rnpB predicted protein/RNase P, RNA component precursor RnpB
?AP012306 3080522 = 315 (1.020)intergenic (+52/+192) pitA/yhiO phosphate transporter, low‑affinity/predicted universal stress (ethanol tolerance) protein B
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AP012306/2743103‑2743199
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCA  >  AP012306/3080522‑3080582
                                                                                                 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||                                                             
GAATTTGCGTGCTTTTGTTGGCAGGTTAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGGTTTAGATTG                                                                  >  2:2303423/1‑150
                                                     AATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCAGATTGTTGAGTGCGCTTTATTCATGCCGGATGCGGCGTGAACGCCTTATCCTGC                 <  1:4031396/146‑1
                                                     AATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCAGATTGTTGAGTGCGCTTTATTCATGCCGGATGCGGCGTGAACGCCTTATCCTGC                 >  2:4031396/1‑146
                                                      ATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCAGATTGTTGAGTGCGCTTTATTCATGCCGGATGCGGCGTGAACGCCTTATCCTGCCTACA            >  2:2728765/1‑150
                                                      ATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCAGATTGTTGAGTGCGCTTTATTCATGCCGGATGCGGCGTGAACGCCTTATCCTGCCTACA            >  2:546489/1‑150
                                                                CTCAACAATCTGAAACCCGCCGGAACGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCAGATTGTTGAGTGCGCTTTATTCATGCCGGATGCGGCGTGAACGCCTTATCCTGCCTACAAAAGCACGCA  <  1:2728765/150‑1
                                                                CTCAACAATCTGAAACCCGCCGGAACGGCTTATCGGTCAGTTTTACCTGATGTACGTAATAAACCGTTCCGGCGGGTTTCAGATTGTTGAGTGCGCTTTATTCATGCCGGATGCGGCGTGAACGCCTTATCCTGCCTACAAAAGCACGCA  <  1:546489/150‑1
                                                                                                 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||                                                             
GAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AP012306/2743103‑2743199
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAACATGCA  >  AP012306/3080522‑3080582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.