Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306202,4170TG23.7% 710.6 / 29.3 278V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base T (65/147);  new base G (66/0);  total (131/147)
Fisher's exact test for biased strand distribution p-value = 2.26e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 318 total aligned reads displayed.

TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCG  >  AP012306/202268‑202566
                                                                                                                                                     |                                                                                                                                                      
   cAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGAtt                                                                                                                                                     >  1:1504212/1‑150 (MQ=255)
    aaaGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTAttt                                                                                                                                                    <  2:1224997/150‑1 (MQ=255)
    aaaGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGAttt                                                                                                                                                    >  2:2622730/1‑150 (MQ=255)
      aGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGAttttt                                                                                                                                                  >  1:1978895/1‑148 (MQ=255)
       gcgcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTtgtg                                                                                                                                                 <  2:282449/150‑1 (MQ=255)
       gcgcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTtgtg                                                                                                                                                 <  2:27637/150‑1 (MQ=255)
        cgcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGa                                                                                                                                                >  1:2906503/1‑150 (MQ=255)
        cgcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGa                                                                                                                                                >  1:2467150/1‑150 (MQ=255)
         gcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGAc                                                                                                                                               <  2:2069790/150‑1 (MQ=255)
          ccAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACg                                                                                                                                              <  2:954790/150‑1 (MQ=255)
              gATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑Caag                                                                                                                                         <  1:3576776/150‑1 (MQ=255)
               aTTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑Caaga                                                                                                                                        <  1:1814542/150‑1 (MQ=255)
                    tgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCCGGGGGATTTTTGACG‑CAAGAAgggg                                                                                                                                   >  2:1171956/1‑148 (MQ=255)
                      cATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGGTTGCGGCGGCAGGGGGATTTGTGACG‑CAAGAAGGtgtg                                                                                                                                 >  1:949681/1‑150 (MQ=255)
                       atatTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTgg                                                                                                                                <  2:3695743/150‑1 (MQ=255)
                        tatTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACG‑CAAGAAGGTGTGGc                                                                                                                               >  1:4152562/1‑150 (MQ=255)
                         atTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGGCACCCCGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGGGGGAGGGGGATTTTTGGCG‑CAAGAAggggggggc                                                                                                                              >  2:456697/1‑143 (MQ=255)
                         atTGTCGGGCGTAACGGCGGAAGGAAGCTAAAAAAAATTTTGCAAAGGCTGGGGGGGCCGCCGTGGCTACGGGACACCACGCCACTGCTGTTTTTTGGCGAAACGCTGATTGCGGGGGGGGGGGGAATTGTGACGCCAAGAAgggtgggt                                                                                                                               >  2:3182207/1‑144 (MQ=255)
                          ttGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTg                                                                                                                             <  1:398360/150‑1 (MQ=255)
                            gTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGGCG‑CCAGAAAGggtgggtgga                                                                                                                           >  2:183288/1‑148 (MQ=255)
                              cGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGGGTGCCGCCGGGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGGAGGGGGATTTGTGACG‑CAAGAAGGGGTGGGTGGAgg                                                                                                                         >  2:3403581/1‑150 (MQ=255)
                              cGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAgg                                                                                                                         <  1:623534/150‑1 (MQ=255)
                                ggCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTg                                                                                                                       <  2:3768608/150‑1 (MQ=255)
                                  cGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTgag                                                                                                                     >  1:1598333/1‑150 (MQ=255)
                                     aaCGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAAt                                                                                                                  >  1:409685/1‑150 (MQ=255)
                                       cggcggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATgg                                                                                                                <  1:2327842/150‑1 (MQ=255)
                                        ggcggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGc                                                                                                               <  1:1324911/150‑1 (MQ=255)
                                          cggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGt                                                                                                             <  1:1899087/150‑1 (MQ=255)
                                          cggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACG‑CAAGAAGGTGTGGCTGAAAGTGAGGATGGCgg                                                                                                             >  2:4051622/1‑149 (MQ=255)
                                           ggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTa                                                                                                            <  1:360227/150‑1 (MQ=255)
                                            gACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTaa                                                                                                           <  2:1652044/150‑1 (MQ=255)
                                               gTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGtt                                                                                                        <  2:1130400/150‑1 (MQ=255)
                                                tAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGttt                                                                                                       <  1:3041290/150‑1 (MQ=255)
                                                tAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGttt                                                                                                       >  1:2513362/1‑150 (MQ=255)
                                                 aaGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGtttt                                                                                                      <  1:1248081/150‑1 (MQ=255)
                                                  aGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTg                                                                                                     <  1:2034551/150‑1 (MQ=255)
                                                    cTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTc                                                                                                   >  1:3782028/1‑150 (MQ=255)
                                                    cTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACG‑CAAGAAGGGGTGGCTGAAGGTGAGAATGGCGGAAGTTTTGTc                                                                                                   >  2:3016286/1‑150 (MQ=255)
                                                      aaaGAAAATCTGGCAGGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTg                                                                                                 <  2:4166294/150‑1 (MQ=255)
                                                      aaaGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTg                                                                                                 >  2:3101212/1‑150 (MQ=255)
                                                       aaGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTgg                                                                                                <  2:1944769/150‑1 (MQ=255)
                                                        aGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGc                                                                                               >  1:2005119/1‑150 (MQ=255)
                                                        aGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGGATGGCGTAAGTTTTGTCTGGc                                                                                               >  1:557638/1‑150 (MQ=255)
                                                          aaaaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAg                                                                                             >  2:3630323/1‑150 (MQ=255)
                                                           aaaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGa                                                                                            <  1:1642215/150‑1 (MQ=255)
                                                           aaaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGa                                                                                            >  1:4220085/1‑150 (MQ=255)
                                                            aaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGaa                                                                                           >  1:828726/1‑150 (MQ=255)
                                                            aaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGaa                                                                                           <  1:1966734/150‑1 (MQ=255)
                                                             aTCTGGCAAGCGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGaaa                                                                                          <  2:1573517/150‑1 (MQ=255)
                                                                tGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACG‑CAAGAAGGTGTGGCTGGAGGGGAGAATGGCGTAAGGTTTGTCTGGCAGAAAACg                                                                                       >  2:1055871/1‑150 (MQ=255)
                                                                  gCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCt                                                                                     <  1:1165867/150‑1 (MQ=255)
                                                                   cAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCtt                                                                                    <  1:3376709/150‑1 (MQ=255)
                                                                     agagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAg                                                                                  <  1:639018/150‑1 (MQ=255)
                                                                     agagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAg                                                                                  >  2:2654920/1‑150 (MQ=255)
                                                                      gagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGGGAGGATGGCGTAAGGTTTGTCTGGCAGAAAACGCTTAGt                                                                                 >  2:247808/1‑150 (MQ=255)
                                                                        gCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATAGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTa                                                                               <  1:644044/150‑1 (MQ=255)
                                                                         cTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTaa                                                                              >  1:266248/1‑150 (MQ=255)
                                                                         cTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTaa                                                                              <  2:2717659/150‑1 (MQ=255)
                                                                           gggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGt                                                                            <  1:2778871/150‑1 (MQ=255)
                                                                             gCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGa                                                                          <  2:569805/150‑1 (MQ=255)
                                                                                tGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAg                                                                       <  2:1385397/150‑1 (MQ=255)
                                                                                   cgccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCg                                                                    <  1:3667166/150‑1 (MQ=255)
                                                                                    gccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCgg                                                                   <  2:4211167/150‑1 (MQ=255)
                                                                                          gCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGa                                                             <  1:176552/150‑1 (MQ=255)
                                                                                             aCGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGc                                                          <  1:3066074/150‑1 (MQ=255)
                                                                                              cGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCa                                                         >  1:3278823/1‑150 (MQ=255)
                                                                                               gTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCAt                                                        >  2:1878999/1‑150 (MQ=255)
                                                                                                 gACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCa                                                      <  1:857696/150‑1 (MQ=255)
                                                                                                 gACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCa                                                      <  1:490730/150‑1 (MQ=255)
                                                                                                   caccacGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCaaa                                                    >  2:645613/1‑150 (MQ=255)
                                                                                                   caccacGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTCAGTTAAGTGAAAGCCGGATAAGACGCATCaaa                                                    >  2:869214/1‑150 (MQ=255)
                                                                                                    accacGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAAc                                                   >  1:4062922/1‑150 (MQ=255)
                                                                                                    accacGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAAc                                                   <  1:1202835/150‑1 (MQ=255)
                                                                                                       acGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTc                                                <  1:2178099/150‑1 (MQ=255)
                                                                                                                 tgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGTGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCg                                      <  2:732752/150‑1 (MQ=255)
                                                                                                                   ttttATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGaa                                    >  2:767873/1‑150 (MQ=255)
                                                                                                                    tttATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGaaa                                   >  2:2265219/1‑150 (MQ=255)
                                                                                                                    tttATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGaaa                                   >  2:3145974/1‑150 (MQ=255)
                                                                                                                     ttATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAg                                  <  2:3590586/150‑1 (MQ=255)
                                                                                                                      tATGGCGAAACGCTGATTGCGGCGGCATGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACg                                                  >  1:2242130/1‑133 (MQ=255)
                                                                                                                      tATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGt                                 <  1:3713617/150‑1 (MQ=255)
                                                                                                                         ggCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCaa                              <  1:1723495/150‑1 (MQ=255)
                                                                                                                         ggCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGGGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCaa                              >  1:102852/1‑150 (MQ=255)
                                                                                                                          gCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACG‑CAAGAAGGGGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAAt                             >  2:3295171/1‑150 (MQ=255)
                                                                                                                           cGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATc                            <  2:159713/150‑1 (MQ=255)
                                                                                                                            gAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGTGAAAGTCAATCa                           <  2:3369992/150‑1 (MQ=255)
                                                                                                                             aaaCGCTGATTGCGGCGGCAGGGGGATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAg                          >  2:3558095/1‑150 (MQ=255)
                                                                                                                                 gCTGATTGCGGCGGCCGGGGGATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGGCTGGGAGAAAACGATTAGTTAAGTGAAACCCGGATAAGACGCATCAAACGTCCCATCCGGCGAAAGTCAAACAGGAtt                      >  2:2050067/1‑150 (MQ=255)
                                                                                                                                 gCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGAtt                      >  1:2729814/1‑150 (MQ=255)
                                                                                                                                   tGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCg                    <  2:3717548/150‑1 (MQ=255)
                                                                                                                                     aTTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCt                  <  1:3938848/150‑1 (MQ=255)
                                                                                                                                      ttGTGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCtt                 <  1:2970701/150‑1 (MQ=255)
                                                                                                                                      ttGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCtt                 <  1:2262316/150‑1 (MQ=255)
                                                                                                                                      ttGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCtt                 >  1:3187563/1‑150 (MQ=255)
                                                                                                                                       tGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTa                <  2:3928353/150‑1 (MQ=255)
                                                                                                                                         cggcggCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTacc              <  2:3469522/150‑1 (MQ=255)
                                                                                                                                               cAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccaccacc        >  2:914932/1‑150 (MQ=255)
                                                                                                                                                aGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCg       <  2:2208297/150‑1 (MQ=255)
                                                                                                                                                aGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCg       >  2:350430/1‑150 (MQ=255)
                                                                                                                                                    gTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTAcc   <  1:1110155/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACG‑CAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCG  >  AP012306/202268‑202566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑