Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306341,3770TG28.3% 235.4 / 18.4 127G60G (GGT→GGG) ribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
Reads supporting (aligned to +/- strand):  ref base T (20/71);  new base G (36/0);  total (56/71)
Fisher's exact test for biased strand distribution p-value = 1.31e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 201 total aligned reads displayed.

CTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCG  >  AP012306/341228‑341526
                                                                                                                                                      |                                                                                                                                                     
cTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAATGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGt                                                                                                                                                       <  2:1994542/150‑1 (MQ=255)
 tAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTg                                                                                                                                                      <  2:1881811/150‑1 (MQ=255)
    aGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGaaa                                                                                                                                                   <  2:763602/150‑1 (MQ=255)
        ggCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGc                                                                                                                                               <  2:2374002/150‑1 (MQ=255)
        ggCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGgaaaaaa                                                                                                                                               >  2:188462/1‑148 (MQ=255)
         gcgcAACGAGGACGTTTTACCACGCATCCCAGCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGcc                                                                                                                                              <  2:2503787/150‑1 (MQ=255)
         gcgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGcc                                                                                                                                              <  1:1088092/150‑1 (MQ=255)
         gcgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGcc                                                                                                                                              <  1:2906038/150‑1 (MQ=255)
          cgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCa                                                                                                                                             <  2:1954033/150‑1 (MQ=255)
          cgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCa                                                                                                                                             <  1:3301233/150‑1 (MQ=255)
           gcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCaa                                                                                                                                            >  1:3626296/1‑150 (MQ=255)
             aaCGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAAACaaaa                                                                                                                                          >  1:1412547/1‑149 (MQ=255)
              aCGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAGGCCAAAgg                                                                                                                                         <  1:2084730/150‑1 (MQ=255)
                gAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTg                                                                                                                                       <  1:264977/150‑1 (MQ=255)
                  ggACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCg                                                                                                                                     <  1:2980296/150‑1 (MQ=255)
                   gACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGGAAAAAACaaaaaggcga                                                                                                                                    >  2:1252415/1‑143 (MQ=255)
                      gTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAAACAAAAGGGCGGCCg                                                                                                                                 >  2:3658677/1‑150 (MQ=255)
                       ttttACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGTGCGACCGc                                                                                                                                >  1:3868425/1‑150 (MQ=255)
                        tttACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAAGGGCGACCGcc                                                                                                                               >  1:3851968/1‑150 (MQ=255)
                          tACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTa                                                                                                                             <  1:2891813/150‑1 (MQ=255)
                            ccACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCGGCCCCCCATg                                                                                                                           >  1:1480016/1‑150 (MQ=255)
                            ccACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAATGCGACCGCCCATg                                                                                                                           >  1:2630599/1‑150 (MQ=255)
                            ccACGCACCCCACCCCGAATGTCGGGTGCGTCATTGTAAAAGAGGGCGAAATTGTCGGTGAAGGTTCCCACCAACGGGCGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTATGGG‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATg                                                                                                                           <  2:522408/150‑1 (MQ=255)
                            ccACGCACCCCACCCCAAATGTGGGGTGCGTCTTTGAAAAAGAGGGCGAAATTGTGGGTGAAGTTTACCACAAACGGGGGGGTGAACCACAGGCCGAAGTACCCGCGTGGGGTAGGGG‑GGGTAAAAAGGCAAAAGGTGCGACCGCCTATg                                                                                                                           <  2:3108021/150‑1 (MQ=255)
                             cACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTCCCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGt                                                                                                                          <  1:1347777/150‑1 (MQ=255)
                              aCGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTCCCCCCAGCGGGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGG‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTc                                                                                                                         <  2:692538/150‑1 (MQ=255)
                              aCGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAAGGGGGACCGCCTCTGTc                                                                                                                         >  1:344517/1‑150 (MQ=255)
                                gCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGGGGCCGCCCAttttcc                                                                                                                       >  2:1289771/1‑145 (MQ=255)
                                  aTCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCCAAAGGGCGGCCCCCCATGTCacac                                                                                                                     >  1:3937121/1‑150 (MQ=255)
                                       aaCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAAGGGCGACCCCCCATTTCCCACTCCaa                                                                                                                >  1:2267518/1‑150 (MQ=255)
                                       aaCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGCGACCGCCTATGTTACACCCGaa                                                                                                                >  1:1815820/1‑150 (MQ=255)
                                       aaCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACAAAAGAGGGGACCGCCCATGGCACCCTCGaa                                                                                                                >  2:78884/1‑150 (MQ=255)
                                          ccGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAAGCCAAA‑GAGCGACCGCCTAATTCCCACTCCGAccc                                                                                                             >  1:788772/1‑150 (MQ=255)
                                          ccGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTGGCGTAGGGC‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAAccc                                                                                                             <  1:2226474/150‑1 (MQ=255)
                                            gAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAAGGGCGACCGCCCATGTCACACTCCAAccccg                                                                                                           >  1:1898027/1‑148 (MQ=255)
                                            gAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGGGGCCGCCCCTGTCCCACCCCAAccccg                                                                                                           >  2:2925721/1‑148 (MQ=255)
                                             agtggggggTGCGTCTTTGTCAAAGATGGCGAAAGTGTCGGTGAAGGTTCCCACCAACGTGCGGGTGAACCACATGCCGAAGCACACGCGTGGCGTAGGGG‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGt                                                                                                          <  2:3925619/144‑1 (MQ=255)
                                                gTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCCAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGc                                                                                                       >  1:1587285/1‑150 (MQ=255)
                                                 tgggggGCGTCATTGAAAAAGAGGGCAAAATTGTGGGTGAGGGTTACCACCAACGGGGGGGTGACCCACATGCCGAAGTACACGCGTTGCGTAGGGG‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGcc                                                                                                      <  2:2758834/148‑1 (MQ=255)
                                                   ggggGCGTCATTGTCAAAGATGGCAAAATTGTCGGTGAAGGTTACCCCCAACGTGGGGGTGACCCACATGCCGAAGTACACGCGTTGCGTAGGGG‑GGGTAAAAAACCAAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCcat                                                                                                    <  2:387329/150‑1 (MQ=255)
                                                   ggggGCGTCATTGTAAAAGATGGCAAAATTGTGGGTGAAGTTTCCCACCACCGGGCGGGTAAACCACATGCCGAAGTACCCGGGTTGCGTGGGGG‑GGGAAAAAAAGCAAAGGGTGCGCCCGCCTATGTCACACTCGAACCCTGTAGCcat                                                                                                    <  2:86547/150‑1 (MQ=255)
                                                    ggTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGTGCGACCCCCTATGTCACCCTCCCACCCTGTAGCcatc                                                                                                   >  2:2255619/1‑150 (MQ=255)
                                                     gTGCGTCATTGTAAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCcatca                                                                                                  <  2:2968098/150‑1 (MQ=255)
                                                      tGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCcatcat                                                                                                 <  1:3394717/150‑1 (MQ=255)
                                                       gCGTCATTGTCAAAGATGGCGAAATTGTGGGTGAAGGTTACCACCAACGGGCGGGTGACCCACAGGCCGAAGTACCCGCGTTGCGTAGGGC‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATg                                                                                                <  2:2587143/150‑1 (MQ=255)
                                                         gTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGGAAAAACCAAAAGGGCGACCCCCTATGTCCCACCCCAACCCCGTAACCCACATGGt                                                                                              >  1:1998649/1‑150 (MQ=255)
                                                         gTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGCCAAAGGGGCGACCGCCCATGTCCCACCCGAACCCCGTAGCCACCACGGt                                                                                              >  2:3436611/1‑150 (MQ=255)
                                                            aTTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAAGGGCGACCGCCTATGTCACACTCGAACCCCGTAGCCATCATGGTCGt                                                                                           >  1:1644314/1‑150 (MQ=255)
                                                            aTTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGCGACCGCCCATTTCACACCCCAACCCCGTAGCCCTCATGGTCGt                                                                                           >  2:830700/1‑150 (MQ=255)
                                                            aTTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCCAAAGTGCGGCCCCCTATGTGCCACTCGCACCCCGTAGCCCCCATGGGCGt                                                                                           >  2:3389089/1‑150 (MQ=255)
                                                            aTTGTAAAAGATGGCGAATTGGTCGGTGAGGTTTACCCCCAAGGTGCGGGTAACCCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGt                                                                                           <  2:2559001/150‑1 (MQ=255)
                                                             ttGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGCGACCGCCCATGTCACACCCCAACCCCGTAGCCATCATGGTCGtt                                                                                          >  2:1673250/1‑149 (MQ=255)
                                                             ttGTAAAAGATGGCGAATTTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTATGGC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTa                                                                                          <  1:315221/150‑1 (MQ=255)
                                                               gTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAAGGGCGACCCCCTATGTTACACCCCAACCCCGTAGCCCTCATGGTCGTACg                                                                                        >  1:3229099/1‑150 (MQ=255)
                                                                 cAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAAACAAAAGAGCGACCGCCTCTGTCCCACTCGAACCCCCTAGCCCTCATGGTCGTACGcc                                                                                      >  2:2198742/1‑150 (MQ=255)
                                                                      aTGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGt                                                                                 <  1:3269903/150‑1 (MQ=255)
                                                                       tGGCAAAATTGTCGGTGAAGGTTACCCCCAACGGGCGGGTGACCCACATGCCGAAGTACACGCGTTGCGTAGGGG‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTg                                                                                <  2:236105/150‑1 (MQ=255)
                                                                         gcaaaaTTGTCGGGGAAGTTTACCACAAACGTGGGGGTGAACCAAATGCCGAAGTCCACGCGTTGCGTTTGGC‑GGGTAAAAAACCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCt                                                                              <  2:3197848/147‑1 (MQ=255)
                                                                           gAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGGAAAAAACAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTGCGCCACCCTGCtgt                                                                            >  2:2989276/1‑150 (MQ=255)
                                                                            aaaTTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCtgtg                                                                           <  1:478390/150‑1 (MQ=255)
                                                                            aaaTTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAGGCGGGACCGCCTCTTTCCCAATCGAACCCCGTAACCCTCCTGGTCGTACGGCACCCGGCtgtg                                                                           >  2:3291104/1‑150 (MQ=255)
                                                                             aaTTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGa                                                                          >  1:838312/1‑150 (MQ=255)
                                                                              atggTCGGGGAAGGTTCCCCCCAACGGGCGGGGGAACCACATGCCGAAGCACACGCGTGGCGTATGGG‑GGTTAAAAAACCAAAAGGGGCGTCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGAc                                                                         <  2:635939/147‑1 (MQ=255)
                                                                               ttGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACg                                                                        <  1:3798097/150‑1 (MQ=255)
                                                                               ttGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTGGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACg                                                                        <  1:3535000/150‑1 (MQ=255)
                                                                                 gTCGGTGAAGGTTACCACCAACGGGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCa                                                                      <  1:571076/150‑1 (MQ=255)
                                                                                     gTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGTGCGACCCCCCATGTCACACTCCAACCCCGTAGCCATCATGGTCGTACGCCCCCCCGCTGTGGCGCAcccc                                                                  >  2:2689094/1‑147 (MQ=255)
                                                                                      agatggTTCCCCCCATCGTGCGGGTAACCAAAATGCCAAAGTACACGCTTTCCGTATGGG‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCAt                                                                 <  2:1229214/146‑1 (MQ=255)
                                                                                       gagggTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATc                                                                <  1:2798491/147‑1 (MQ=255)
                                                                                        aaGGTTCCCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATcg                                                               <  2:1201197/150‑1 (MQ=255)
                                                                                        aaGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGG‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATcg                                                               <  1:2204984/150‑1 (MQ=255)
                                                                                         aGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATcgc                                                              <  1:3890682/150‑1 (MQ=255)
                                                                                           gtcaccaccAACGGGCGGGTAACCCACAGGCCTAAGTACCCGCGTTGCGTAGGGG‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATcgccg                                                            <  2:2801886/147‑1 (MQ=255)
                                                                                                 acaaaCGGGCGGGTGAACCACATGCAGAAGTACCCGCGTTGCGTATGGG‑GGGTAAAAAAGCAAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCg                                                      <  2:1205757/147‑1 (MQ=255)
                                                                                                    aaCGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAg                                                   >  1:374212/1‑150 (MQ=255)
                                                                                                               gAACCACATGCCGAAGTACCGGCGTGGCGTAGGGC‑GGGTAAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGtt                                        <  1:2811768/150‑1 (MQ=255)
                                                                                                                aaCCACAGGCCGAAGTACCCGCGTGGCGTAGGGC‑GGGTAAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTg                                       <  2:2950247/150‑1 (MQ=255)
                                                                                                                 acaacaTGCCGAAGTACCCGCGTTGGGTAGGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGc                                      <  1:1180594/147‑1 (MQ=255)
                                                                                                                    acaTGCCGAAGTACACGGGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTCGCCTc                                   <  1:2689094/150‑1 (MQ=255)
                                                                                                                    acaTGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTc                                   <  1:304345/150‑1 (MQ=255)
                                                                                                                     taTGCAGAAGTACGGGCGTGGCGTATGGC‑GGGTAAAAACGCAAAAGTTGCGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCg                                  <  1:2373778/149‑1 (MQ=255)
                                                                                                                      aTGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAAGGGCGACCGCCCATGTCACACTCGAACCCCGTAGCCATCATGGTCGTACGCCACCGTGCTGGGACGCACCCATCGCCCCCGGCGTAGCGCGCGGGGGTGCCTcca                                 >  2:2459216/1‑148 (MQ=255)
                                                                                                                        gCCGAAGTACGCGCGTGGCGTAGGGG‑GGGTGAAAAAGCAAAGGGTGGGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATg                               <  1:4135347/150‑1 (MQ=255)
                                                                                                                         cggAAGTACCCGCGTGGCGTAGGGG‑GGGTAAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGc                              <  2:3033962/148‑1 (MQ=255)
                                                                                                                         ccGAAGTACACGCGTGGCGTAGGGC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGc                              <  1:3169314/150‑1 (MQ=255)
                                                                                                                          cGAAGTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa                             <  2:3557977/150‑1 (MQ=255)
                                                                                                                             aGTACCCGCGTTGCGTAGGGC‑GGGTGAAAAACCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGa                          <  1:857415/150‑1 (MQ=255)
                                                                                                                                acacGCGTGGTGTAGGGG‑GGGGAAAAAAGCAAAGGGTGTGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCGCGATGCAAGATcc                       <  2:3827073/150‑1 (MQ=255)
                                                                                                                                 cacGCGTGGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCt                      <  1:3500280/150‑1 (MQ=255)
                                                                                                                                   cgcgTTGCGTAGGGG‑GGGTAAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTaa                    <  2:410406/150‑1 (MQ=255)
                                                                                                                                     cgTTGCGTATGGC‑GGGTGAAAAAACCAAAGGTGCGACCCCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAAcc                  >  1:3782097/1‑150 (MQ=255)
                                                                                                                                        tGCGTATGGC‑GGGGGAAAAAACCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCCTCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGCGGGTGCCTCGATGCAAGATCCTAACCCGc               >  2:3355190/1‑150 (MQ=255)
                                                                                                                                        tGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCGACCGCCCATGTCACACTCGAACCCCGTAGCCATCATGGGCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCCAGATCCTAAccccc               >  2:445186/1‑148 (MQ=255)
                                                                                                                                           gTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAgg            <  1:4019866/150‑1 (MQ=255)
                                                                                                                                               ggC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGc        <  1:1300911/150‑1 (MQ=255)
                                                                                                                                                gC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCt       >  2:3756119/1‑150 (MQ=255)
                                                                                                                                                 c‑GGGTGAAACACCGAACGGTGGGGCCGACTATGTCACACTCGATCCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTg      <  2:399668/150‑1 (MQ=255)
                                                                                                                                                    ggTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTggg    >  2:1062236/1‑150 (MQ=255)
                                                                                                                                                    ggTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTggg    >  2:2038339/1‑150 (MQ=255)
                                                                                                                                                     gTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGc   >  1:4054418/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCG  >  AP012306/341228‑341526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑