Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306935,0540GT26.0% 276.0 / 42.5 96intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
Reads supporting (aligned to +/- strand):  ref base G (13/58);  new base T (25/0);  total (38/58)
Fisher's exact test for biased strand distribution p-value = 7.20e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 177 total aligned reads displayed.

CAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAT‑CGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAAC  >  AP012306/934907‑935202
                                                                                                                                                    |                                                                                                                                                      
cAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTtttttt                                                                                                                                                      >  1:2475935/1‑150 (MQ=255)
cAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTtttgtt                                                                                                                                                      >  2:3705105/1‑150 (MQ=255)
cAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGtttttt                                                                                                                                                      >  2:661634/1‑150 (MQ=255)
 aGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGtttttttt                                                                                                                                                     >  2:416324/1‑150 (MQ=255)
 aGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTtttgttt                                                                                                                                                     <  2:3795741/150‑1 (MQ=255)
   tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGtttttttttg                                                                                                                                                   >  1:3365277/1‑148 (MQ=255)
   tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTgg                                                                                                                                                   <  2:1459375/150‑1 (MQ=255)
     ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCt                                                                                                                                                 >  1:416360/1‑150 (MQ=255)
     ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCt                                                                                                                                                 <  2:3595912/150‑1 (MQ=255)
     ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGCt                                                                                                                                                 >  2:3388084/1‑150 (MQ=255)
      tGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCtt                                                                                                                                                >  2:576697/1‑150 (MQ=255)
       gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTc                                                                                                                                               >  2:2849212/1‑150 (MQ=255)
       gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGGTTc                                                                                                                                               >  1:3500081/1‑150 (MQ=255)
        ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGttttttttttgttca                                                                                                                                              >  2:501056/1‑143 (MQ=255)
        ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGtttttttgtgtttt                                                                                                                                              >  2:364766/1‑144 (MQ=255)
        ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGCtttt                                                                                                                                              >  2:3028410/1‑148 (MQ=255)
         gATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTcag                                                                                                                                             >  1:3183172/1‑150 (MQ=255)
         gATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTccg                                                                                                                                             >  1:3976566/1‑148 (MQ=255)
         gATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTGGCTTccg                                                                                                                                             >  1:389399/1‑148 (MQ=255)
           ttGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTcagca                                                                                                                                           <  1:3160614/150‑1 (MQ=255)
           ttGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGGTTGTTTGGCTTcgtct                                                                                                                                           >  2:311825/1‑146 (MQ=255)
            tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGCTTTTTTTGGCtttaaccg                                                                                                                                          >  1:2436873/1‑144 (MQ=255)
             gTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTTagcagc                                                                                                                                         >  1:2250437/1‑150 (MQ=255)
             gTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTGTTTTGCTTCtgcagc                                                                                                                                         >  2:3180410/1‑150 (MQ=255)
              tAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑g                                                                                                                                       <  2:2458230/150‑1 (MQ=255)
                 tgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGt                                                                                                                                    <  1:2988704/150‑1 (MQ=255)
                  gtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTTACCAGC‑ggggt                                                                                                                                   >  2:2110077/1‑148 (MQ=255)
                    ttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCTGCCGC‑GGGGTgg                                                                                                                                 >  2:326716/1‑149 (MQ=255)
                    ttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCTGCAGC‑GGGGTgt                                                                                                                                 >  2:1124992/1‑149 (MQ=255)
                    ttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTTGCTTTCGCAGC‑GGGGTgc                                                                                                                                 >  1:865180/1‑150 (MQ=255)
                     tAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTGTTTGGCTTTCGCAGC‑GGGGTgcg                                                                                                                                >  1:2890894/1‑150 (MQ=255)
                      aGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCAGCAGC‑GGGGTgcgg                                                                                                                               >  1:2064220/1‑149 (MQ=255)
                      aGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCCTCAGCAGC‑GGGGTgcgc                                                                                                                               >  1:3183340/1‑150 (MQ=255)
                       gTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCAGCAGC‑GGGGTGCGCt                                                                                                                              >  1:2601169/1‑150 (MQ=255)
                       gTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCt                                                                                                                              <  1:3231160/150‑1 (MQ=255)
                          tGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTTTGCAGC‑CGGGTTCGCcccc                                                                                                                           >  2:3299501/1‑150 (MQ=255)
                           gCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCCGCAGC‑GGGGTGCGCTCCCt                                                                                                                          >  1:1262929/1‑150 (MQ=255)
                           gCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCt                                                                                                                          <  1:611854/150‑1 (MQ=255)
                           gCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGGTTTTGCAGC‑GGGGTGGGCTcccc                                                                                                                          >  2:2674675/1‑149 (MQ=255)
                            cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGATTTTTTTTGGTTTAGCAGC‑GGGGTGGGCTCCCtt                                                                                                                         >  1:912192/1‑150 (MQ=255)
                             tGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGGTTTTTTTTGGTTTTA‑CAGC‑GGGGTGCGCTCCCCTa                                                                                                                        >  2:424321/1‑150 (MQ=255)
                             tGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCAGCAGC‑GGGTTGCGCTCCCTTa                                                                                                                        >  1:1749634/1‑150 (MQ=255)
                             tGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTa                                                                                                                        <  1:3900898/150‑1 (MQ=255)
                              gggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTTTGCAGC‑GGGGTTTGCTCCCttta                                                                                                                       >  2:745223/1‑148 (MQ=255)
                              gggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTaa                                                                                                                       <  1:2647798/150‑1 (MQ=255)
                               ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAAt                                                                                                                      <  1:3536080/150‑1 (MQ=255)
                                gcaaaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTGTTTTGCTTCTGCAGC‑GGGGTGCGCTCCCCTACtg                                                                                                                     >  1:1927292/3‑150 (MQ=255)
                                 tAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGTTTTCGCCAC‑GGGGTGGCCTCCCCTAAttt                                                                                                                    >  2:3549231/1‑148 (MQ=255)
                                  aaaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTGGGTTCAGCAGC‑GGGGTGCGCCCCCCTTAtgtg                                                                                                                   >  1:667859/1‑150 (MQ=255)
                                       gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTTAGCAGC‑GGGGTGCGCTCCCCTTATGTGGCTCg                                                                                                              >  1:48262/1‑150 (MQ=255)
                                       gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCAGCAGC‑GGGGTGCGCTCCCCTAATGTGGCTCg                                                                                                              >  2:2058377/1‑150 (MQ=255)
                                        ccGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGCTTCAGCAGC‑GGGGTGCGGCCCCCTTATTTGcccccg                                                                                                             >  2:2936324/1‑146 (MQ=255)
                                         cGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTTGCTTCTGCCGC‑GGGGTTCCCTCCCCTTATTTGCCTcccc                                                                                                            >  1:2700121/1‑150 (MQ=255)
                                         cGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTTGCTTCAGCAGC‑GGGGTGCGCTCCCCTTATGTGCCTCGcc                                                                                                            >  1:1545404/1‑150 (MQ=255)
                                           tCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTCAGCAGC‑GGGGTGCGCGCCCCTAAAGTTCCCCGcccc                                                                                                          >  1:1419857/1‑148 (MQ=255)
                                            cAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCata                                                                                                         <  1:285494/150‑1 (MQ=255)
                                            cAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCata                                                                                                         <  2:563296/150‑1 (MQ=255)
                                            cAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGCTTTTGCAAC‑GGGGTGGGCTCCCCTTATGTGGCTCGccctt                                                                                                         >  2:3744311/1‑147 (MQ=255)
                                             aGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTTGTTCCGCAGC‑GGGGTTCGGTCCCTTTATTTGCCTccgcctct                                                                                                        >  2:1579481/1‑143 (MQ=255)
                                              gcggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCAtata                                                                                                       <  1:696712/150‑1 (MQ=255)
                                               cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCAGCAGC‑GGGGTGCCCCCCCCTCATATGCCCCCCCatatta                                                                                                      >  2:706082/1‑148 (MQ=255)
                                               cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATaa                                                                                                      <  1:1031880/150‑1 (MQ=255)
                                               cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGCTTCAGCAGC‑GGGGGGCGCTCCCCTAATGGGTCTCCCCCTATaa                                                                                                      >  1:1300193/1‑150 (MQ=255)
                                               cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGCTTCAGCAAC‑GGGGTGGGCTCCCTTTATGTGACCCGCCCTATaa                                                                                                      >  1:1864101/1‑150 (MQ=255)
                                                  cgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCTGCAGC‑GGGGTGCGCTCCCCTTATTTTCCTCCCCCCATaaaat                                                                                                   >  1:549727/1‑148 (MQ=255)
                                                  cgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGGTTTAGCAGC‑GGGGTGGGCTCCCCTAATGTGCCTCCCCATTTAAAtt                                                                                                   >  1:3813051/1‑150 (MQ=255)
                                                   gATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTTTGCAGC‑GGGGTGCGCGCCCTTTATGTGCCTCGCCCTATaaaatt                                                                                                  >  2:1385722/1‑147 (MQ=255)
                                                   gATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTg                                                                                                  <  1:1129479/150‑1 (MQ=255)
                                                    aTGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCCGCAGC‑GGGGTGCGCCCCCCTTATTTGCCTCCCCCTATaaaattg                                                                                                 >  1:4122635/1‑146 (MQ=255)
                                                      gCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑CGGGTGCGCCCCCCTTATGTGCCTCGCCCTATAAAATTAAt                                                                                               >  2:1259412/1‑150 (MQ=255)
                                                        aaaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTTAGCAGC‑GGGGTGCGCTCCCCTTATGTGGCTCGCCCTATAAATTTAAttg                                                                                             >  2:3605007/1‑148 (MQ=255)
                                                        aaaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATgg                                                                                             <  1:177521/150‑1 (MQ=255)
                                                         aaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTCAGCAGCGGGGTGGGGCCCCCTTATTTTGCC‑CCCCCTATAAAATTAATggg                                                                                            >  2:1834895/1‑149 (MQ=255)
                                                              gCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTTGCGTCAGCGGC‑CGGGTGCGGCCCCCCCATTTGGCGCGCCACCTTAATTGAATGGTggggg                                                                                       >  2:1733206/1‑146 (MQ=255)
                                                                           cGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTTAGCAGC‑GGGGTGCGCCCCCCTTATTTGCCTCGCCCTATAAATTTAATGGTGCCGGGGGCGCGCCgggg                                                                          >  2:2249685/1‑150 (MQ=255)
                                                                            gATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTTAGCAGC‑GGGGTGCGCTCCCCTTATGTGCCTCGCCCTATAAATTTAATGGTGCAGGGAGCGCGCCggggg                                                                         >  2:2351158/1‑150 (MQ=255)
                                                                              tGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGggcg                                                                       <  1:1458252/150‑1 (MQ=255)
                                                                                cgcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCTGCAGC‑GGGGTGCGCTCCCCTTATGTGCCTCGCCCTATAAAATTAATGGGGCAGGGGGCGCGCCGGGggcggc                                                                     >  2:397022/1‑150 (MQ=255)
                                                                                   aCTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGGTTTAGCAGC‑GGGGTGCGCCCCCCTTATTTGGCCCGCCCTATTAAATTAATTGGGCGGGGGGGGCGCGgggggggggcca                                                                  >  2:2136005/1‑146 (MQ=255)
                                                                                            cGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCAGCAGC‑GGGGTGGGCCCCCCTTATGTGGCTCCCCCTATAAATTGTAAGGTGGGGGGGGCGCGCCGGGGGGGGGCAAT‑agccgccc                                                        >  2:3781495/1‑149 (MQ=255)
                                                                                              gCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCCGCAGC‑GGGGTGGGCTCCCCTAATGTGCCTCCCCCTATAAATTTAATGGGGCAGGGGGGGCGCCGGGGGGGGGCCAA‑Cgcccccgcc                                                      >  2:1513565/1‑150 (MQ=255)
                                                                                                         aCGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAT‑CGCCGCCGCCCCCTGCTGTcc                                           <  2:2152135/150‑1 (MQ=255)
                                                                                                           ggTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGGTTCAGCAGC‑GGGGTGGGCTCCCCTAATTTGCCTCCCCCTATAAATTGAATGGGGCAGGGGGCGCGCAGGGGGGGGGCCAA‑CGCCGCCGCCCCCCCCTGTCCCg                                         >  2:2623287/1‑150 (MQ=255)
                                                                                                             ttGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAT‑CGCCGCCGCCCCCTGCTGTCCCGGc                                       <  1:3935446/150‑1 (MQ=255)
                                                                                                              tGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAT‑CGCCGCCGCCCCCTGCTGTCCCGGcc                                      <  2:1882983/150‑1 (MQ=255)
                                                                                                                  ggAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAT‑CGCCGCCGCCCCCTGCTGTCCCGGCCTTCg                                  <  1:2410550/150‑1 (MQ=255)
                                                                                                                      aGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCCGCAAC‑CGGGTTCGGCCCCCTAAAGTAGCTCTGCCTATAAAATTTATGGTGGCGGGGGGGCGCTGGGGGGGGCCCATCCCCCCCCCCCCCCTCCGGTCCCGCCCTCCgggg                               >  2:681066/1‑150 (MQ=255)
                                                                                                                      aGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAT‑CGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGa                              <  2:1112771/150‑1 (MQ=255)
                                                                                                                         tAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAGATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAT‑CGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACg                           <  2:3322418/150‑1 (MQ=255)
                                                                                                                              gACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAT‑CGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCa                      <  2:926971/150‑1 (MQ=255)
                                                                                                                              gACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAT‑CGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCa                      <  2:2722793/150‑1 (MQ=255)
                                                                                                                                    gCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAT‑CGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGAtt                <  1:1347690/150‑1 (MQ=255)
                                                                                                                                         cggc‑ggTTTTTTTTTGCTTCAACAGC‑GGGGTGCGCTCCCTTAATTTGCCTCGCCCTATAAATTTAATGGTGCAGGGAGCGCGCAGGGGGGGGGCAAT‑CGCCCCCCCCCCCCGCTGTCCCCGCCCTTGGGGGACGCTTTAACGGTTTTTAc           >  2:3844743/1‑150 (MQ=255)
                                                                                                                                          ggc‑ggTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAT‑CGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACg          <  1:1619785/150‑1 (MQ=255)
                                                                                                                                           gc‑ggTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAT‑CGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGc         >  2:3926952/1‑150 (MQ=255)
                                                                                                                                           gc‑ggTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGAAGGGGGCGGCCAAT‑CGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGc         >  2:628808/1‑150 (MQ=255)
                                                                                                                                            c‑ggTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAT‑CGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGcc        <  2:1510913/150‑1 (MQ=255)
                                                                                                                                               gTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAT‑CGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccac      <  1:1030584/150‑1 (MQ=255)
                                                                                                                                                ttttGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAT‑CGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacc     >  2:803694/1‑150 (MQ=255)
                                                                                                                                                   tgtttgGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAT‑CGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAac  >  1:3656216/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
CAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAT‑CGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAAC  >  AP012306/934907‑935202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑