Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,854,8510AC26.2% 679.7 / 75.1 282T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
Reads supporting (aligned to +/- strand):  ref base A (184/24);  new base C (0/74);  total (184/98)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 310 total aligned reads displayed.

TAGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTGGGGC  >  AP012306/1854702‑1854998
                                                                                                                                                     |                                                                                                                                                   
    ggTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAAcccc                                                                                                                                                 >  2:1425847/1‑150 (MQ=255)
      tCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTg                                                                                                                                               <  2:2659043/150‑1 (MQ=255)
       cTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGc                                                                                                                                              >  1:742934/1‑150 (MQ=255)
          ataaCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGt                                                                                                                                           >  1:4091592/1‑150 (MQ=255)
           taaCATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGtt                                                                                                                                          >  2:2881892/1‑150 (MQ=255)
                tCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGt                                                                                                                                     <  1:3068379/150‑1 (MQ=255)
                      aaGGGAAAAAGATCCATGGCATACTATAAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCACCCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGcc                                                                                                                               <  1:2366606/150‑1 (MQ=255)
                        gggAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACCCTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCa                                                                                                                             <  2:2669696/150‑1 (MQ=255)
                        gggAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCa                                                                                                                             >  1:3934217/1‑150 (MQ=255)
                        gggAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCa                                                                                                                             >  1:3953617/1‑150 (MQ=255)
                        gggAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCa                                                                                                                             >  1:3972713/1‑150 (MQ=255)
                           aaaaaGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCg                                                                                                                          >  1:527275/1‑150 (MQ=255)
                            aaaaGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCgg                                                                                                                         <  1:686086/150‑1 (MQ=255)
                             aaaGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGCCCATGATTTTTACCCTTCGCCCATATCACCAAGAAGCCGTGGATGCCACGCTCACCCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGgc                                                                                                                        <  2:3615887/150‑1 (MQ=255)
                                gATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCa                                                                                                                     >  1:258645/1‑150 (MQ=255)
                                 aTCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAg                                                                                                                    >  2:711835/1‑150 (MQ=255)
                                 aTCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAg                                                                                                                    >  2:346924/1‑150 (MQ=255)
                                  tCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAgg                                                                                                                   >  2:3788891/1‑150 (MQ=255)
                                     aTGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTaa                                                                                                                >  1:2307470/1‑150 (MQ=255)
                                     aTGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTaa                                                                                                                >  1:1428369/1‑150 (MQ=255)
                                       ggCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTaaaa                                                                                                              >  1:2554007/1‑150 (MQ=255)
                                        gCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAg                                                                                                             >  1:1910413/1‑150 (MQ=255)
                                         cATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGc                                                                                                            >  2:2144080/1‑150 (MQ=255)
                                         cATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGc                                                                                                            >  2:1949635/1‑150 (MQ=255)
                                          aTACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGcc                                                                                                           <  1:3498881/150‑1 (MQ=255)
                                          aTACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGcc                                                                                                           >  2:1166735/1‑150 (MQ=255)
                                          aTACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGcc                                                                                                           >  2:1245511/1‑150 (MQ=255)
                                          aTACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGcc                                                                                                           >  2:1766667/1‑150 (MQ=255)
                                          aTACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGcc                                                                                                           >  2:3573141/1‑150 (MQ=255)
                                          aTACTATTAGCAGAATAATATACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGcc                                                                                                           >  1:625233/1‑150 (MQ=255)
                                           tACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAATCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCt                                                                                                          >  1:458381/1‑150 (MQ=255)
                                            aCTATTAGCAAAATAATCTACCTCCGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTCCGTCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTg                                                                                                         <  2:3366192/150‑1 (MQ=255)
                                             cTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTgg                                                                                                        >  1:1387818/1‑150 (MQ=255)
                                                ttAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGa                                                                                                     >  2:3940767/1‑150 (MQ=255)
                                                   gCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAg                                                                                                  >  1:1739866/1‑150 (MQ=255)
                                                     aGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCg                                                                                                <  1:936998/150‑1 (MQ=255)
                                                     aGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCg                                                                                                >  1:2428968/1‑150 (MQ=255)
                                                          aatCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACt                                                                                           >  1:2193648/1‑150 (MQ=255)
                                                            tCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTgg                                                                                         <  2:2256772/150‑1 (MQ=255)
                                                            tCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTgg                                                                                         <  2:3677149/150‑1 (MQ=255)
                                                            tCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTgg                                                                                         >  1:355764/1‑150 (MQ=255)
                                                             ctacctacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGc                                                                                        >  2:3237653/1‑150 (MQ=255)
                                                               ccctccGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCAc                                                                                      <  2:860197/149‑1 (MQ=255)
                                                                  tacGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACggc                                                                                   <  1:3481361/150‑1 (MQ=255)
                                                                   acGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACggct                                                                                  >  2:1570761/1‑150 (MQ=255)
                                                                    cgcgAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACggctg                                                                                 >  2:3706901/1‑150 (MQ=255)
                                                                          cccATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGt                                                                           <  1:1361539/149‑1 (MQ=255)
                                                                          aCCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGt                                                                           >  1:3328395/1‑150 (MQ=255)
                                                                          aCCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGt                                                                           >  2:3867981/1‑150 (MQ=255)
                                                                           ccATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTg                                                                          >  1:1820910/1‑150 (MQ=255)
                                                                           ccATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTg                                                                          >  2:129468/1‑150 (MQ=255)
                                                                           ccATGATTTTTACACTTCCCCTATACCGGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTg                                                                          <  2:3297659/150‑1 (MQ=255)
                                                                             aTGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGt                                                                        >  1:1039164/1‑150 (MQ=255)
                                                                                   tttACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtg                                                                  >  1:2912786/1‑150 (MQ=255)
                                                                                      acacTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctg                                                               <  1:2042032/150‑1 (MQ=255)
                                                                                        acTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctggt                                                             >  2:1084305/1‑150 (MQ=255)
                                                                                         cTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgctggtg                                                            >  2:2496326/1‑150 (MQ=255)
                                                                                         cTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCgtgccgggg                                                            >  2:2014744/1‑148 (MQ=255)
                                                                                             gCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGtgctggtgctgg                                                        <  2:1481564/150‑1 (MQ=255)
                                                                                             gCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGtgctggtgctgg                                                        >  1:1779624/1‑150 (MQ=255)
                                                                                               ctatagCACCAAGAACCCGTGGATCCCACGCCCAACCATTTTCGTCGCCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGca                                                      <  2:3433749/148‑1 (MQ=255)
                                                                                               ccATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGca                                                      >  2:4129904/1‑150 (MQ=255)
                                                                                               ccATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGca                                                      >  2:547864/1‑150 (MQ=255)
                                                                                                cATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGcac                                                     >  2:620933/1‑150 (MQ=255)
                                                                                                 atatCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTACCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGGGCTGGTGCTGGcaca                                                    >  2:650166/1‑150 (MQ=255)
                                                                                                 atacCAGAAAGAAGCCGTGGATGCCCCGCCCACCCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGcaca                                                    <  2:793571/150‑1 (MQ=255)
                                                                                                  tatCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCacac                                                   <  1:1470603/150‑1 (MQ=255)
                                                                                                    tCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGt                                                 <  1:794322/150‑1 (MQ=255)
                                                                                                     cAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTAATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGtt                                                <  2:1103019/150‑1 (MQ=255)
                                                                                                      aGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGGCGCGTGCTGGTGCTGGCACACGTTa                                               >  2:4040420/1‑150 (MQ=255)
                                                                                                      aGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCAAAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTa                                               <  1:209143/150‑1 (MQ=255)
                                                                                                       gCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTaa                                              <  1:1503312/150‑1 (MQ=255)
                                                                                                        cAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTaaa                                             >  1:989833/1‑150 (MQ=255)
                                                                                                         aagaagCCGTGGATGCCACGCTCAACCATTTTCGTCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAg                                            <  2:4167077/150‑1 (MQ=255)
                                                                                                           gaagCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGaa                                          >  1:562723/1‑150 (MQ=255)
                                                                                                           aaagCCGTGGATGCCACGCTCAACCATTTCCGTCGTAATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGaa                                          <  1:1576615/149‑1 (MQ=255)
                                                                                                            aagCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAAc                                         <  2:3200545/150‑1 (MQ=255)
                                                                                                             gcccGTGTATGCCCCGCCTAACCTTTCTGCTCATCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACt                                        <  2:2393525/148‑1 (MQ=255)
                                                                                                             agCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACt                                        <  2:2203072/150‑1 (MQ=255)
                                                                                                              gCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACtg                                       <  2:1373668/150‑1 (MQ=255)
                                                                                                              gCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACtg                                       <  1:2698323/150‑1 (MQ=255)
                                                                                                                 gTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACtggtg                                    >  1:3611681/1‑150 (MQ=255)
                                                                                                                   ggATGCCACGCTCAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGgc                                  <  1:1159551/150‑1 (MQ=255)
                                                                                                                   ggATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGgc                                  >  2:2729210/1‑150 (MQ=255)
                                                                                                                    gATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGgcg                                 >  2:160027/1‑150 (MQ=255)
                                                                                                                    gATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGgcg                                 >  1:362816/1‑150 (MQ=255)
                                                                                                                          aCGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAAc                           >  1:3773072/1‑150 (MQ=255)
                                                                                                                             cTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCAt                        >  2:3052634/1‑150 (MQ=255)
                                                                                                                              tCAACCATCTCCGTCTTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATg                       <  2:2099861/150‑1 (MQ=255)
                                                                                                                               cAACCATTTTCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGc                      <  1:1106751/150‑1 (MQ=255)
                                                                                                                                     ttttCGTCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTa                <  2:190685/150‑1 (MQ=255)
                                                                                                                                       ttCGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATc              <  2:291714/150‑1 (MQ=255)
                                                                                                                                        tcgtcgTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCa             <  2:2630932/150‑1 (MQ=255)
                                                                                                                                         cgtcgtAATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAg            <  2:478555/150‑1 (MQ=255)
                                                                                                                                              tcATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCt       >  1:2799971/1‑150 (MQ=255)
                                                                                                                                               cATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTg      >  1:2987239/1‑150 (MQ=255)
                                                                                                                                                aTAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTgg     >  1:1999932/1‑150 (MQ=255)
                                                                                                                                                 tAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTggg    >  1:2957102/1‑150 (MQ=255)
                                                                                                                                                  aaacCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTgggg   <  1:176461/150‑1 (MQ=255)
                                                                                                                                                  aaaaCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTgggg   >  1:1602029/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TAGCGGTCTAATAACATCCCTCAAGGGAAAAAGATCCATGGCATACTATTAGCAGAATAATCTACCTACGCGAGACCATGATTTTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTTCGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACGGCTGGCTCGTGGTCGCGTGCTGGTGCTGGCACACGTTAAAGAACTGGTGGCGCAAAACCATGCAAAGTATCAGGCGCTGGGGC  >  AP012306/1854702‑1854998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑