Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,020,6700TG31.7% 281.7 / 11.5 163K195N (AAA→AAC) glgCglucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (73/37);  new base G (51/0);  total (126/37)
Fisher's exact test for biased strand distribution p-value = 1.03e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 242 total aligned reads displayed.

ATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTCTTGCTT  >  AP012306/3020521‑3020819
                                                                                                                                                     |                                                                                                                                                       
gatctaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGt                                                                                                                                                         <  1:4211328/145‑1 (MQ=255)
 tCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGtt                                                                                                                                                        <  1:839055/150‑1 (MQ=255)
 tCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGtt                                                                                                                                                        <  2:2791623/150‑1 (MQ=255)
  cAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGgtt                                                                                                                                                       >  2:4189532/1‑150 (MQ=255)
   aaaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGtttt                                                                                                                                                      <  1:555254/150‑1 (MQ=255)
   aaaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGtttt                                                                                                                                                      <  1:3306336/150‑1 (MQ=255)
   aaaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGAAGCAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGCCGGCGGGGTGGCCGGgttt                                                                                                                                                      >  2:2605687/1‑150 (MQ=255)
    aaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGCTAGCAGGgtttt                                                                                                                                                     >  1:2775715/1‑150 (MQ=255)
       cTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTTACAGGTTTTTTaa                                                                                                                                                  >  2:1604107/1‑150 (MQ=255)
        tttGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑c                                                                                                                                                <  1:274330/150‑1 (MQ=255)
          tGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTTGCAGGgttttttacaa                                                                                                                                               >  2:456947/1‑144 (MQ=255)
           gCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGCTAGCCGGGTTTTTaaacaa                                                                                                                                              >  2:61361/1‑146 (MQ=255)
            ccAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAAt                                                                                                                                            <  1:1799385/150‑1 (MQ=255)
            ccAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAAt                                                                                                                                            >  1:3742316/1‑150 (MQ=255)
             cAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAAtt                                                                                                                                           >  1:3844674/1‑150 (MQ=255)
              aaaGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTc                                                                                                                                          <  2:1292948/150‑1 (MQ=255)
              aaaGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTc                                                                                                                                          <  1:2216353/150‑1 (MQ=255)
                aGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGa                                                                                                                                        >  1:768618/1‑150 (MQ=255)
                 gTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTTAA‑CGAATTTGAt                                                                                                                                       >  1:2529110/1‑150 (MQ=255)
                 gTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGATTTTTCA‑CGAATTTGAt                                                                                                                                       >  2:1708009/1‑150 (MQ=255)
                    gTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAAt                                                                                                                                    <  1:1730134/150‑1 (MQ=255)
                    gTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTCCA‑CGGAATTGATaaa                                                                                                                                    >  1:2018205/1‑149 (MQ=255)
                    gTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTCAA‑CGAATTCGATAAt                                                                                                                                    >  1:1264756/1‑150 (MQ=255)
                     tGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTCAA‑CGAATTCGATAAtt                                                                                                                                   >  1:1109376/1‑150 (MQ=255)
                      ggCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAA‑CGAATTCGATAAttt                                                                                                                                  >  2:846417/1‑150 (MQ=255)
                      ggCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTCAA‑CGAATTCGATAAttt                                                                                                                                  >  1:3609622/1‑150 (MQ=255)
                          ggAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATc                                                                                                                              >  1:3017379/1‑150 (MQ=255)
                            aGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGt                                                                                                                            <  2:284001/150‑1 (MQ=255)
                             gTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGtt                                                                                                                           <  2:3425471/150‑1 (MQ=255)
                             gTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGAAGGTTTTTCAA‑CGAATTTGATAAATTTATCGtt                                                                                                                           >  1:1877234/1‑150 (MQ=255)
                              ttCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAAATTTATCGTtc                                                                                                                          >  1:1174185/1‑150 (MQ=255)
                               tctcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTtct                                                                                                                         <  2:3299642/150‑1 (MQ=255)
                               tctcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTtct                                                                                                                         <  2:3662311/150‑1 (MQ=255)
                                 tcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTTAA‑CGAATTTGATAATTTTATCGTTCtc‑a                                                                                                                      >  2:1534595/1‑150 (MQ=255)
                                  cATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAA‑CGAATTCGATAATTTTATCGTTCtc‑at                                                                                                                     >  1:2401383/1‑150 (MQ=255)
                                   aTCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCtc‑atc                                                                                                                    <  1:3233380/150‑1 (MQ=255)
                                   aTCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCtc‑atc                                                                                                                    >  1:941531/1‑150 (MQ=255)
                                   aTCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCtc‑atc                                                                                                                    <  1:2187243/150‑1 (MQ=255)
                                   aTCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAA‑CGAATTCGATAATTTTATCGTTCtc‑att                                                                                                                    >  2:2720586/1‑149 (MQ=255)
                                    tCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCtc‑atca                                                                                                                   <  1:1038230/150‑1 (MQ=255)
                                      gcgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAAGGTTTTCAA‑CGAATTCGATAATTTTATCGGTCTC‑CTCAAc                                                                                                                 >  2:118386/1‑150 (MQ=255)
                                      gcgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTAGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAAc                                                                                                                 <  1:716529/150‑1 (MQ=255)
                                       cgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAAcc                                                                                                                >  1:167094/1‑150 (MQ=255)
                                        gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCg                                                                                                               <  1:2857624/150‑1 (MQ=255)
                                        gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTCAA‑CGAAATTGATAATTTTATCGTTCTC‑ATCAACCg                                                                                                               >  2:959727/1‑150 (MQ=255)
                                          tCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGcc                                                                                                             >  1:3189381/1‑150 (MQ=255)
                                             tcttctTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCaca                                                                                                          >  2:2215977/1‑148 (MQ=255)
                                             tcttctTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATa                                                                                                          >  1:2314010/1‑150 (MQ=255)
                                                tcttcCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCCTAACg                                                                                                       >  1:2111738/1‑150 (MQ=255)
                                                tcttcCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTTAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATaaag                                                                                                       >  2:2089981/1‑148 (MQ=255)
                                                  ttcCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGcc                                                                                                     >  1:633662/1‑150 (MQ=255)
                                                      agcagTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAA‑CGAATTCGATAATTTTTTCGTTCTC‑ATCAACCCCCATAACGCCAAAt                                                                                                 >  2:966941/1‑150 (MQ=255)
                                                      agcagTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTTAA‑CGAATTCGATAATTTTTTCGTTCTC‑ATCAACCGCCATAACGCCAAAt                                                                                                 >  2:2472866/1‑150 (MQ=255)
                                                      agcagTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTCAA‑CGAATTTGATAATTTTATCGTTTTC‑ATCAACCGCCATAACGCCAAAt                                                                                                 >  2:3960932/1‑150 (MQ=255)
                                                         agTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCg                                                                                              >  1:3077601/1‑150 (MQ=255)
                                                            tCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTTTCGTTCTC‑ATCAACCGCCATAACGCCAAATGCgggg                                                                                           >  2:3796202/1‑148 (MQ=255)
                                                            tCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCgggg                                                                                           >  2:2693137/1‑148 (MQ=255)
                                                             cATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAgg                                                                                          >  2:665060/1‑150 (MQ=255)
                                                               tACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCCTAACGCCAAATACGGAGGca                                                                                        >  2:3106876/1‑149 (MQ=255)
                                                               tACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTCAA‑TGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGct                                                                                        >  1:3952190/1‑150 (MQ=255)
                                                                    aTAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCttcttc                                                                                   >  1:2664991/1‑150 (MQ=255)
                                                                    aTAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAAATCGATAATTTTTTCGTTCTC‑ATCAACCGCCATAACGCCAAATGCCGAGGGttcttc                                                                                   >  2:1932568/1‑150 (MQ=255)
                                                                    aTAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTTAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCttcttc                                                                                   >  2:874531/1‑150 (MQ=255)
                                                                    aTAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCttcttc                                                                                   >  1:2138534/1‑150 (MQ=255)
                                                                      aGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCaa                                                                                 >  1:1415588/1‑150 (MQ=255)
                                                                      aGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAA‑CCAATTCGATAATTTTTTCTGTCTC‑ATCAACCGCCATAAAGACAAATGCGGGGGCCTCCTCaa                                                                                 >  2:2666053/1‑150 (MQ=255)
                                                                              tCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACt                                                                         >  1:1787199/1‑150 (MQ=255)
                                                                                   gACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTTAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAAAGCGGAGGCTTCTTCAATCGGTACTGGCAt                                                                    >  2:3600604/1‑150 (MQ=255)
                                                                                      atAGATACCCATCCCCGCCAGAGTTTTGCGCGGTTCTTTCGGCATTGACGGGGGGTTAGGAGTTTTTCCAA‑CAATTTCGATATTTTTTTCTTTCTC‑ATAACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACa                                                                 <  2:3766448/149‑1 (MQ=255)
                                                                                          aTACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCa                                                             >  2:596976/1‑150 (MQ=255)
                                                                                              ccATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACgg                                                         >  2:2203308/1‑150 (MQ=255)
                                                                                                   cTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCaa                                                    >  2:3174335/1‑150 (MQ=255)
                                                                                                      gCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAA‑CGAAATTGATAATTTTTTTGTTCTC‑CTCAACCCCCATAAAGCCAAAAGCGGAGGCTTCTTCAATTGGGACTGGCATACAAACAACGGGGCAACGt                                                 >  2:2017523/1‑150 (MQ=255)
                                                                                                       ccAGAGTTTTGCTGGGATCTTTCGGCATTGGGGGGGGGTTAGGAGTTTTTCAAA‑CGAATTCGATATTTTTTTCTTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTg                                                <  2:3699979/150‑1 (MQ=255)
                                                                                                         agagATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTTAA‑CGAATTTGATAATTTTTTCGTTCTC‑ATCAACCGCCCTAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAACAACGGTGCAACGTgcg                                              >  2:3026835/1‑150 (MQ=255)
                                                                                                                 gCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTc                                      >  1:1579932/1‑150 (MQ=255)
                                                                                                                     ggATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAAAGGTGCAACGTGCGCCTTTTTCGACg                                  >  1:303370/1‑150 (MQ=255)
                                                                                                                     ggATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAA‑AGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGGGCAACGTGCGCCCTTTTCGACg                                  >  2:1558393/1‑150 (MQ=255)
                                                                                                                         cGTTCGGTATGGGGGGGGGGTTAGCAGTTTTTTCAA‑CAAATTAGATATTTTTATCTTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGAt                              <  2:2508352/150‑1 (MQ=255)
                                                                                                                         cGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAA‑CGAATTCGATAATTTTTTCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGAt                              >  2:215407/1‑150 (MQ=255)
                                                                                                                             cGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGAt                          >  1:367073/1‑150 (MQ=255)
                                                                                                                              ggCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTTTCGTTTTC‑ATCAACCGCCATAACGCCAAATGCGGGGGCTTCTTCAATCGGTATTGGCATACAAACAACGGTGCAACGTGCGCCTTTTTTGACGTGATCGATa                         >  2:1863621/1‑150 (MQ=255)
                                                                                                                              ggCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATa                         >  1:3502314/1‑150 (MQ=255)
                                                                                                                                   ggggggggggTTAGCTGTTTTTCAAA‑CAAATTCGATATTTTTATCTTTCTC‑ACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCAt                    <  2:2263634/143‑1 (MQ=255)
                                                                                                                                    gACGGCGGGTTAACAGGGTTTTCAA‑CAAATTCGATAATTTTTTCGTTCTC‑ATCAACCGCCCTAACACCCAATGCGGAGGCTTCTTCTATCGGTACTGGCATACAAGCAACGGTGCCACGTGGGCCTTTTTCGACGTGATCGTTAAGCaaa                   >  2:3080610/1‑148 (MQ=255)
                                                                                                                                     ggggggggTTAGCAGTTTTTACAA‑CAAATTCGATATTTTTTTCTTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATAc                  <  2:1103692/145‑1 (MQ=255)
                                                                                                                                     cgggggggTTAGTA‑GTTTTTCAACCAAATTAATTATTTTTTTCTTTCCA‑ACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATAc                  <  2:384065/145‑1 (MQ=255)
                                                                                                                                      ggggggtttAGCTGTTTTTTAAA‑CAATTTTAATATTTTTTCCTTTCTC‑ACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATAcg                 <  2:3818694/143‑1 (MQ=255)
                                                                                                                                       ggcggGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATAcgc                >  1:3397669/1‑150 (MQ=255)
                                                                                                                                        gggggTTTGCTGGTTTTCCAC‑CAAATTGAATATTTTTATCGTTCCC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATAcgcg               <  1:729473/148‑1 (MQ=255)
                                                                                                                                        gggggTTAGCATTTTTTTCAA‑AAAATTAAATATTTTTTTCGTTCTC‑ACCACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATAcgcg               <  2:2767180/148‑1 (MQ=255)
                                                                                                                                         ggggTTAGCATTTTTTACAA‑AAAATTCGATAATTTTATATTCCTA‑ACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGa              <  1:87153/149‑1 (MQ=255)
                                                                                                                                          gtgTTAGCA‑GTTTTTCAA‑AGGATTAAAATATTTTTTCCTTCCCAAACAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGag             <  2:2645277/148‑1 (MQ=255)
                                                                                                                                          gggTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGag             >  1:2081441/1‑150 (MQ=255)
                                                                                                                                             ttAGCAGGATTTTCAA‑CGAATTCTATAATTTTATCGTTCTC‑ATCAACCCCCATAACGCCAAATGCGAAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTTATCGATAAGCATACGCGagtag          >  2:499277/1‑150 (MQ=255)
                                                                                                                                             tcaGCAGGTTTTTCAA‑CAAATTCAATAATTTTACCTTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagtag          <  1:472926/148‑1 (MQ=255)
                                                                                                                                                gCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTct       >  1:4072638/1‑150 (MQ=255)
                                                                                                                                                 cAGGTTTTCAAA‑CGATTAAAATAATTTTATCTTCCTA‑ATCAGCCGCCATAACGCCAAATGCGGAGGCTTCTTCAACCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTctt      <  1:4025814/150‑1 (MQ=255)
                                                                                                                                                     tttttCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttgctt  >  2:3370458/1‑150 (MQ=255)
                                                                                                                                                     ttttcCAA‑CAAATTCGATATTTTTCTCGTTCTC‑ACAACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttgctt  <  2:4087715/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
ATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAA‑CGAATTCGATAATTTTATCGTTCTC‑ATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTCTTGCTT  >  AP012306/3020521‑3020819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑