Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,527,3680AC31.7% 495.1 / 72.0 240L140F (TTA→TTC) rhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
Reads supporting (aligned to +/- strand):  ref base A (136/28);  new base C (0/76);  total (136/104)
Fisher's exact test for biased strand distribution p-value = 2.73e-39
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 284 total aligned reads displayed.

CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTG  >  AP012306/3527219‑3527517
                                                                                                                                                      |                                                                                                                                                     
cGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTa                                                                                                                                                       <  1:2872084/150‑1 (MQ=255)
cGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTa                                                                                                                                                       >  2:369774/1‑150 (MQ=255)
  aTTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTAcc                                                                                                                                                     >  2:100459/1‑150 (MQ=255)
   tttCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTAccc                                                                                                                                                    >  1:2260482/1‑150 (MQ=255)
     tCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTc                                                                                                                                                  >  2:58269/1‑150 (MQ=255)
      cAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCg                                                                                                                                                 >  1:4213682/1‑150 (MQ=255)
         tttCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGAcc                                                                                                                                              >  2:2312452/1‑150 (MQ=255)
          ttCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTTGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCg                                                                                                                                             <  2:398693/150‑1 (MQ=255)
          ttCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCg                                                                                                                                             <  1:3516327/150‑1 (MQ=255)
            ctcGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGcc                                                                                                                                           >  1:2741710/1‑150 (MQ=255)
             tcGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCa                                                                                                                                          <  1:1950897/150‑1 (MQ=255)
               gCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGCGAAAATGA‑TTTACCCTCGACCGCCAGt                                                                                                                                        >  1:3626912/1‑150 (MQ=255)
                     cTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCgag                                                                                                                                  >  1:4049535/1‑150 (MQ=255)
                          tCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCGGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCtt                                                                                                                             <  1:1853044/150‑1 (MQ=255)
                            ggtccctgcCACAAGAGATGGATGAGCAGTTGCCTTCCCTCGGGCGCTTTATCCACCGTGTATTCGAGCAGGCGCGAATGTTGCTGGCAAACAGGGATGAGAAGGAA‑GGAAAAATGACTTTCCCCCCGCCCGCCAGTCGCGAGATCTTGt                                                                                                                           <  2:1340889/145‑1 (MQ=255)
                             gtggctgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGGGTTAACCACAGCGTATGGCAGCAGGGGCGACAGCGGTTTGCACAGAGGGAACAGCGGGAAGGGGAAAATGT‑TTTCCCCCCGACCGCCAGTCGCGAGATCTTGtt                                                                                                                          <  2:3836492/147‑1 (MQ=255)
                             gTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGtt                                                                                                                          <  1:492441/150‑1 (MQ=255)
                             gTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGtt                                                                                                                          >  1:121475/1‑150 (MQ=255)
                              ttGCTGCCACAAGAGCTGGATGGTCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGttt                                                                                                                         >  1:2029382/1‑150 (MQ=255)
                              ttGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGttt                                                                                                                         >  2:492578/1‑150 (MQ=255)
                                 ctgcAACAAGAGCTGGAGGGGCAGTACCCGTCTCACGGGCGCGTTAACCACAGCTTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAGGGGAAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATg                                                                                                                      <  2:961640/150‑1 (MQ=255)
                                  tgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGc                                                                                                                     <  1:101831/150‑1 (MQ=255)
                                   gcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCa                                                                                                                    >  2:366590/1‑150 (MQ=255)
                                   gcCACAAGAGCGGGATGGGCAGTATCCGTCTCACGGGCGCGTTAACCACAGCTTATTGCAGCAGGTGCGACATCTGGTTGCACAGATGGAAAAGCGGGAGGGGAAAAAAGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCa                                                                                                                    <  2:2490408/150‑1 (MQ=255)
                                   gcCAAAAGGGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTACCCACAGGGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGAAGGAGGGGGAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCa                                                                                                                    <  2:84773/150‑1 (MQ=255)
                                    ccaaaggagCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTACCCACAGCGTATGGCGGGAGGTGCGACAGCTGGTTGAACAGATGGAACAGCAGAAAGGGAAAAATGT‑TTTCCCCTCGCCCGCCAGTCGCGAGATCTTGTTTATGCaa                                                                                                                   <  2:4132583/144‑1 (MQ=255)
                                    ccACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCaa                                                                                                                   >  1:1645864/1‑150 (MQ=255)
                                       caAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTTGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTa                                                                                                                <  2:34959/150‑1 (MQ=255)
                                          gagCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTg                                                                                                             <  1:3260041/150‑1 (MQ=255)
                                           agCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGc                                                                                                            >  1:131624/1‑150 (MQ=255)
                                             cTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctc                                                                                                          >  1:24693/1‑150 (MQ=255)
                                              gggAGGGGCAGTATCCGTCTCACTGGCGCGTTACCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGTACAGATGGAACAGCAGGAAGGGGAAAATGT‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctct                                                                                                         <  2:3585572/149‑1 (MQ=255)
                                               ggATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGAGGGAACAGCAGGAAGGGAAAAATGT‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCtt                                                                                                        <  2:1201982/150‑1 (MQ=255)
                                                gATGGGCGGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCGGGTGCGACGGCTGGTTGCACAGAGGGAACAGCAGGAGGGGGAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtg                                                                                                       <  2:3277952/150‑1 (MQ=255)
                                                  tGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgct                                                                                                     >  1:3427236/1‑150 (MQ=255)
                                                  tGGGCAGTACCCGTCCCACTGGCGCGTTAACCACAGCGTTTTGCAGCGGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAGGGGAAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgct                                                                                                     <  2:2914601/150‑1 (MQ=255)
                                                      cAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGt                                                                                                 <  2:440854/150‑1 (MQ=255)
                                                         tATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaa                                                                                              <  1:3320526/150‑1 (MQ=255)
                                                         tATCCGTCCCACTGGCGCGTTACCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaa                                                                                              <  1:3912779/150‑1 (MQ=255)
                                                          aTCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaaa                                                                                             >  2:1275844/1‑150 (MQ=255)
                                                           tCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAg                                                                                            >  1:242731/1‑150 (MQ=255)
                                                            ccGTCTCACTGGGGCGTTAACCACGGCGTATTGCAGGAGGGGCGACAGTGGGTTGCACAGAGGGAACAGCGGGAAGGGGAAAATGA‑TTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGc                                                                                           <  2:198246/150‑1 (MQ=255)
                                                                ctcACTGGCGCGTTAACCACAGCGTATTGCAGCAGGGGCGACAGCTGGTTGCACAGAGGGAACAGAAGGAAGGGGAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGtt                                                                                       <  2:1845891/150‑1 (MQ=255)
                                                                 tcACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGttt                                                                                      >  1:539221/1‑150 (MQ=255)
                                                                  cACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTg                                                                                     <  1:410609/150‑1 (MQ=255)
                                                                  cACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTg                                                                                     <  1:3773159/150‑1 (MQ=255)
                                                                   aCTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGc                                                                                    >  1:2640510/1‑150 (MQ=255)
                                                                   aCTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGc                                                                                    <  2:2565906/150‑1 (MQ=255)
                                                                     tGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAg                                                                                  <  1:1077953/150‑1 (MQ=255)
                                                                     tGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAg                                                                                  <  1:2121485/150‑1 (MQ=255)
                                                                       gcgcGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGga                                                                                >  1:3545988/1‑150 (MQ=255)
                                                                        cgcgTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgag                                                                               <  1:1432108/150‑1 (MQ=255)
                                                                        cgcgTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTCCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgag                                                                               <  1:673724/150‑1 (MQ=255)
                                                                         gcgTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGAGGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgaga                                                                              <  1:767010/150‑1 (MQ=255)
                                                                          cgTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAAAAGCAGGAAGGGAAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGaa                                                                             <  2:325081/150‑1 (MQ=255)
                                                                           gTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAAc                                                                            >  2:526725/1‑150 (MQ=255)
                                                                           gTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAAc                                                                            >  2:3192014/1‑150 (MQ=255)
                                                                            ttAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAAcc                                                                           >  1:1158208/1‑150 (MQ=255)
                                                                            ttAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAAcc                                                                           >  1:23862/1‑150 (MQ=255)
                                                                            ttAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAAcc                                                                           >  1:191762/1‑150 (MQ=255)
                                                                            ttAACCACAGCGTATGGCGGCGGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAGGGGGAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAAcc                                                                           <  2:1367213/150‑1 (MQ=255)
                                                                              aaCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTg                                                                         >  2:2956045/1‑150 (MQ=255)
                                                                                ccACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGa                                                                       >  2:2745533/1‑150 (MQ=255)
                                                                                 cacaGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaa                                                                      >  2:2872596/1‑150 (MQ=255)
                                                                                          ttGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCa                                                             >  1:2888124/1‑150 (MQ=255)
                                                                                            ggcgcgggTGCGACAGCTGGTTGCACAGATGGAACAGCGGGAAGGGGAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATc                                                           <  2:774198/144‑1 (MQ=255)
                                                                                             cagcagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCa                                                          >  2:2408681/1‑150 (MQ=255)
                                                                                             cagcagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCa                                                          >  2:3079981/1‑150 (MQ=255)
                                                                                                cagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGt                                                       >  2:105675/1‑150 (MQ=255)
                                                                                                 agGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAGGGGGAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGGGAACCTGGAAAACAGCGCATCACGtc                                                      <  2:2094635/150‑1 (MQ=255)
                                                                                                    tGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCa                                                   <  1:579216/150‑1 (MQ=255)
                                                                                                       gACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTTCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACt                                                >  1:906242/1‑150 (MQ=255)
                                                                                                       gACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACt                                                >  1:2528620/1‑150 (MQ=255)
                                                                                                        aCAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACtt                                               >  1:792705/1‑150 (MQ=255)
                                                                                                         cAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGAAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTg                                              >  2:2061076/1‑150 (MQ=255)
                                                                                                          aGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGc                                             >  1:1754646/1‑150 (MQ=255)
                                                                                                            cTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCtt                                           >  2:2796964/1‑150 (MQ=255)
                                                                                                            cTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCtt                                           >  1:3013742/1‑150 (MQ=255)
                                                                                                                   cacaGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcct                                    >  1:2764611/1‑150 (MQ=255)
                                                                                                                    acaGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctg                                   >  1:3158599/1‑150 (MQ=255)
                                                                                                                    acaGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctg                                   >  2:3862761/1‑150 (MQ=255)
                                                                                                                     caGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctgg                                  >  1:1181217/1‑150 (MQ=255)
                                                                                                                      aGATGGAACAGCAGGAAGGGGAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctggc                                 <  1:1563317/150‑1 (MQ=255)
                                                                                                                             aCAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggagga                          >  1:3209228/1‑150 (MQ=255)
                                                                                                                               agcagGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGAcc                        >  2:3005224/1‑150 (MQ=255)
                                                                                                                                 cgggAGGGGAAAAATGT‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAt                      <  2:1354698/148‑1 (MQ=255)
                                                                                                                                  agGAAGGGGAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAtt                     <  1:2161281/150‑1 (MQ=255)
                                                                                                                                    gagggggAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAtttt                   <  1:3087006/147‑1 (MQ=255)
                                                                                                                                       ggggAAAATGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGcc                <  2:3782262/150‑1 (MQ=255)
                                                                                                                                        ggaaaaaTTT‑TTTCCCCTCCCCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCg               <  2:1602938/147‑1 (MQ=255)
                                                                                                                                        ggaaaaaGGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCg               <  1:4026782/147‑1 (MQ=255)
                                                                                                                                         gaaaaaTGA‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGa              <  2:1545863/148‑1 (MQ=255)
                                                                                                                                          aaaaaTGT‑TTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGAt             <  2:3538481/149‑1 (MQ=255)
                                                                                                                                           aaaaTGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATg            >  2:2524104/1‑150 (MQ=255)
                                                                                                                                           aaaaTGA‑TTCCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATg            <  2:744488/150‑1 (MQ=255)
                                                                                                                                              attattttCCCCGCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAATTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAg          <  2:1799472/145‑1 (MQ=255)
                                                                                                                                                gA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTg       >  2:3394003/1‑150 (MQ=255)
                                                                                                                                                 tgtttCCCCGCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTg       <  2:1409194/148‑1 (MQ=255)
                                                                                                                                                 a‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGa      >  1:1364650/1‑150 (MQ=255)
                                                                                                                                                    ttATCCGCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAAt    <  2:1483586/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGA‑TTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTG  >  AP012306/3527219‑3527517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑