BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	1,438,071	T→A	I264N (ATC→AAC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	1,438,071	0	T	A	100.0%	1220.0 / NA	306	I264N (ATC→AAC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (175/131); total (175/131)

Only 100 of 328 total aligned reads displayed.

TCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTG  >  AP012306/1437924‑1438217

tCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACg                                                                                                                                                  <  1:4094716/150‑1 (MQ=255)

 ccACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGa                                                                                                                                                 <  1:2251955/150‑1 (MQ=255)

     atcatcTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGaaaaa                                                                                                                                             <  2:1756847/150‑1 (MQ=255)

      tcatcTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAAt                                                                                                                                            >  2:354366/1‑150 (MQ=255)

      tcatcTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAAt                                                                                                                                            <  1:2165488/150‑1 (MQ=255)

       catcTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATg                                                                                                                                           >  1:3332165/1‑150 (MQ=255)

       catcTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATg                                                                                                                                           <  2:3451900/150‑1 (MQ=255)

       catcTCCAAAATCGAAAACCAGGAAGGCCTAAACAACTTCGACGAAATCCGCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATg                                                                                                                                           <  2:790954/150‑1 (MQ=255)

          ctcCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTAt                                                                                                                                        >  1:821085/1‑150 (MQ=255)

          ctcCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTAt                                                                                                                                        >  2:105790/1‑150 (MQ=255)

             cAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCg                                                                                                                                     <  1:374004/150‑1 (MQ=255)

              aaaaTCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGt                                                                                                                                    >  1:3212045/1‑150 (MQ=255)

              aaaaTCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGt                                                                                                                                    >  1:763205/1‑150 (MQ=255)

                aaTCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGc                                                                                                                                  <  2:2459578/150‑1 (MQ=255)

                   cGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACg                                                                                                                               >  2:3363070/1‑150 (MQ=255)

                       aaCCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTaaa                                                                                                                           >  1:2079035/1‑150 (MQ=255)

                        aCCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAg                                                                                                                          >  2:1934350/1‑150 (MQ=255)

                        aCCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAg                                                                                                                          <  1:3409245/150‑1 (MQ=255)

                        aCCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAg                                                                                                                          <  1:4215354/150‑1 (MQ=255)

                         ccAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGt                                                                                                                         <  1:133318/150‑1 (MQ=255)

                           aGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCg                                                                                                                       >  2:1393995/1‑150 (MQ=255)

                           aGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCg                                                                                                                       <  2:4038765/150‑1 (MQ=255)

                              aaGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTa                                                                                                                    <  1:386567/150‑1 (MQ=255)

                               aGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTAt                                                                                                                   <  2:3689664/150‑1 (MQ=255)

                                    tCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTg                                                                                                              >  1:2546652/1‑150 (MQ=255)

                                          acTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGAccc                                                                                                        <  2:680997/150‑1 (MQ=255)

                                          acTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGAccc                                                                                                        >  1:1241955/1‑150 (MQ=255)

                                           cTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCa                                                                                                       >  1:3696463/1‑150 (MQ=255)

                                            ttCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAg                                                                                                      >  1:3604159/1‑150 (MQ=255)

                                            ttCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATC‑TCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGa                                                                                                     <  1:2035554/150‑1 (MQ=255)

                                             tCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGa                                                                                                     >  1:3643795/1‑150 (MQ=255)

                                              cgacgaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGAt                                                                                                    <  1:1845221/150‑1 (MQ=255)

                                              cgacgaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGAt                                                                                                    <  2:571224/150‑1 (MQ=255)

                                               gacgaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATg                                                                                                   <  2:2339841/150‑1 (MQ=255)

                                               gacgaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATg                                                                                                   >  2:3604467/1‑150 (MQ=255)

                                                  gaAATCCTCGCAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTg                                                                                                <  1:2759202/150‑1 (MQ=255)

                                                   aaaTCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTgg                                                                                               <  1:2979249/150‑1 (MQ=255)

                                                    aaTCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGa                                                                                              <  1:3835756/150‑1 (MQ=255)

                                                      tCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGAtt                                                                                            >  1:98710/1‑150 (MQ=255)

                                                       ccTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTc                                                                                           >  1:629106/1‑150 (MQ=255)

                                                       ccTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTc                                                                                           <  1:2403332/150‑1 (MQ=255)

                                                         tCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCa                                                                                         >  2:3488895/1‑150 (MQ=255)

                                                          cGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCAt                                                                                        <  1:2298126/150‑1 (MQ=255)

                                                          cGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCAt                                                                                        <  2:3935646/150‑1 (MQ=255)

                                                           gAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATg                                                                                       <  2:3787637/150‑1 (MQ=255)

                                                           gAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATg                                                                                       >  2:3119744/1‑150 (MQ=255)

                                                            aaGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGa                                                                                      >  2:2957340/1‑150 (MQ=255)

                                                             aGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGAt                                                                                     >  2:3407976/1‑150 (MQ=255)

                                                              gCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATc                                                                                    >  2:3707463/1‑150 (MQ=255)

                                                              gCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATc                                                                                    >  1:4278907/1‑150 (MQ=255)

                                                                ctctGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCaa                                                                                  <  1:1972613/150‑1 (MQ=255)

                                                                ctctGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCaa                                                                                  >  2:505516/1‑150 (MQ=255)

                                                                    gACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAAc                                                                              <  2:1449619/150‑1 (MQ=255)

                                                                    gACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAAc                                                                              <  2:2826124/150‑1 (MQ=255)

                                                                     aCGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAAcc                                                                             >  2:7204/1‑150 (MQ=255)

                                                                       ggCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCa                                                                           <  2:916535/150‑1 (MQ=255)

                                                                        gCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCAc                                                                          <  2:2368921/150‑1 (MQ=255)

                                                                         catcatGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACg                                                                         >  2:3301839/1‑150 (MQ=255)

                                                                          atcatGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGc                                                                        <  1:534162/150‑1 (MQ=255)

                                                                           tcatGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGcc                                                                       <  1:1100555/150‑1 (MQ=255)

                                                                            catGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGccc                                                                      <  2:118845/150‑1 (MQ=255)

                                                                             atGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCg                                                                     <  2:4174496/150‑1 (MQ=255)

                                                                                gTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACt                                                                  >  2:2241932/1‑150 (MQ=255)

                                                                                     gcgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCgcgc                                                             >  1:101093/1‑150 (MQ=255)

                                                                                     gcgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCgcgc                                                             >  1:3302190/1‑150 (MQ=255)

                                                                                        tGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGa                                                          <  2:1593116/150‑1 (MQ=255)

                                                                                           cGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGc                                                       >  2:4089821/1‑150 (MQ=255)

                                                                                            gACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGcc                                                      >  2:4301512/1‑150 (MQ=255)

                                                                                             aCCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCg                                                     >  1:1403138/1‑150 (MQ=255)

                                                                                              ccTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                                    <  2:2859425/150‑1 (MQ=255)

                                                                                                 gggTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGa                                                 >  2:1257543/1‑150 (MQ=255)

                                                                                                  ggTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAc                                                >  2:1685401/1‑150 (MQ=255)

                                                                                                        gAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCa                                          >  2:2648050/1‑150 (MQ=255)

                                                                                                        gAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCa                                          >  2:2297099/1‑150 (MQ=255)

                                                                                                          aaTCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCaaa                                        <  2:2515461/150‑1 (MQ=255)

                                                                                                          aaTCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCaaa                                        >  1:1162058/1‑150 (MQ=255)

                                                                                                          aaTCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCaaa                                        >  1:2887207/1‑150 (MQ=255)

                                                                                                             cccGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGc                                     >  1:4178575/1‑150 (MQ=255)

                                                                                                                   agaagaAGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCt                               <  2:2003262/150‑1 (MQ=255)

                                                                                                                    gaagaaGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTc                              <  1:1583467/150‑1 (MQ=255)

                                                                                                                    gaagaaGTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTc                              <  2:322541/150‑1 (MQ=255)

                                                                                                                      agaagTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGa                            >  2:3021527/1‑150 (MQ=255)

                                                                                                                      agaagTTATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGa                            <  2:4237592/150‑1 (MQ=255)

                                                                                                                            tATCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTAc                      >  1:1675850/1‑150 (MQ=255)

                                                                                                                             aTCTTCGCCCAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACt                     >  2:3148206/1‑150 (MQ=255)

                                                                                                                                     ccAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGt             <  1:3048488/150‑1 (MQ=255)

                                                                                                                                      cAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAACCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTg            <  1:4137275/150‑1 (MQ=255)

                                                                                                                                      cAGAAGATGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTg            >  2:2593651/1‑150 (MQ=255)

                                                                                                                                        gaagaTGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGAt          <  1:1705690/150‑1 (MQ=255)

                                                                                                                                         aagaTGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAATCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATg         <  1:3977660/150‑1 (MQ=255)

                                                                                                                                         aagaTGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATg         >  1:1487237/1‑150 (MQ=255)

                                                                                                                                          agaTGATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACAGTACTGACGCAGTGATGc        >  1:3996057/1‑150 (MQ=255)

                                                                                                                                             tgatgaaCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGt     <  2:1838968/150‑1 (MQ=255)

                                                                                                                                             tgatgaaCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGt     >  2:650024/1‑150 (MQ=255)

                                                                                                                                             tgatgaaCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGt     <  2:1549378/150‑1 (MQ=255)

                                                                                                                                             tgatgaaCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGt     >  2:892905/1‑150 (MQ=255)

                                                                                                                                              gatgaaCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTc    >  1:2583977/1‑150 (MQ=255)

                                                                                                                                               atgaaCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCt   >  1:3547747/1‑150 (MQ=255)

                                                                                                                                                tgaaCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTg  <  1:1907779/150‑1 (MQ=255)

                                                                                                                                                tgaaCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTg  >  2:1954129/1‑150 (MQ=255)

TCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTG  >  AP012306/1437924‑1438217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`