BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	2,098,789	G→T	A200A (GCG→GCT)	yfeU →	predicted PTS component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	2,098,789	0	G	T	100.0%	1154.6 / NA	298	A200A (GCG→GCT)	yfeU	predicted PTS component
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (138/160); total (138/160)

Only 100 of 303 total aligned reads displayed.

GAATACGCACGCCAGCTCGGCTGCCGCACAGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCGCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAG  >  AP012306/2098643‑2098935

gAATACGCACGCCAGCTCGGCTGCCGCACAGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAg                                                                                                                                                 >  2:1540941/1‑150 (MQ=255)

gAATACGCACGCCAGCTCGGCTGCCGCACAGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAg                                                                                                                                                 <  1:1789597/150‑1 (MQ=255)

 aaTACGCACGCCAGCTCGGCTGCCGCACAGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGa                                                                                                                                                <  1:260754/150‑1 (MQ=255)

    acgcacgcCAGCTCGGCTGCCGCACAGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAAc                                                                                                                                             <  1:2483151/150‑1 (MQ=255)

      gcacgcCAGCTCGGCTGCCGCACAGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTg                                                                                                                                           >  1:3078164/1‑150 (MQ=255)

      gcacgcCAGCTCGGCTGCCGCACAGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTg                                                                                                                                           <  2:232957/150‑1 (MQ=255)

        acgcCAGCTCGGCTGCCGCACAGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGt                                                                                                                                         >  2:562294/1‑150 (MQ=255)

        acgcCAGCTCGGCTGCCGCACAGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGt                                                                                                                                         <  2:1586810/150‑1 (MQ=255)

        acgcCAGCTCAGCTGCCGCACAGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGt                                                                                                                                         >  1:2966174/1‑150 (MQ=255)

             aGCTCGGCTGCCGCACAGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCa                                                                                                                                    >  1:939773/1‑150 (MQ=255)

              gCTCGGCTGCCGCACAGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCaa                                                                                                                                   >  1:1273257/1‑150 (MQ=255)

                tCGGCTGCCGCACAGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAata                                                                                                                                 <  2:2942299/150‑1 (MQ=255)

                  ggCTGCCGCACAGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATg                                                                                                                               <  1:887699/150‑1 (MQ=255)

                    cTGCCGCACAGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCt                                                                                                                             <  1:2898931/150‑1 (MQ=255)

                        cGCACAGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTcc                                                                                                                         <  2:52334/150‑1 (MQ=255)

                           acaGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCAcc                                                                                                                      >  2:3856073/1‑150 (MQ=255)

                            caGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTACCACCg                                                                                                                     <  2:3704028/150‑1 (MQ=255)

                            caGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCACAATATGCTTTCCACCg                                                                                                                     <  2:325431/150‑1 (MQ=255)

                                gggAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCt                                                                                                                 >  2:2889899/1‑150 (MQ=255)

                                 ggAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCtg                                                                                                                >  1:2091705/1‑150 (MQ=255)

                                    aTTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCtgatg                                                                                                             <  2:1042445/150‑1 (MQ=255)

                                        ccTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTa                                                                                                         <  2:2729760/150‑1 (MQ=255)

                                         cTGTAGTCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTaa                                                                                                        <  1:1114243/150‑1 (MQ=255)

                                            tAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATc                                                                                                     <  2:111743/150‑1 (MQ=255)

                                            tAATCCGGGGAGCGCCGTTTCAACCACCGCTGACTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAACCTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATc                                                                                                     <  2:3051172/150‑1 (MQ=255)

                                             aaTCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGTATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATcc                                                                                                    >  1:2396090/1‑150 (MQ=255)

                                               tCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCgg                                                                                                  <  2:744240/150‑1 (MQ=255)

                                                   gggAGCGCCGTTTCAACCACCGCTGAGTTTACCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCaaa                                                                                              <  2:4140570/150‑1 (MQ=255)

                                                     gAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAgt                                                                                            <  1:2116173/150‑1 (MQ=255)

                                                         gcCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTc                                                                                        >  1:19058/1‑150 (MQ=255)

                                                         gcCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTc                                                                                        >  2:3563323/1‑150 (MQ=255)

                                                          ccGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCg                                                                                       <  1:3534975/150‑1 (MQ=255)

                                                            gTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGc                                                                                     <  2:602747/150‑1 (MQ=255)

                                                            gTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGc                                                                                     >  1:3370668/1‑150 (MQ=255)

                                                               tCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAAc                                                                                  <  1:3009183/150‑1 (MQ=255)

                                                                cAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAAcc                                                                                 >  2:670473/1‑150 (MQ=255)

                                                                  accaccGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCATCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTg                                                                               <  1:181657/150‑1 (MQ=255)

                                                                   ccaccGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGa                                                                              >  1:3885928/1‑150 (MQ=255)

                                                                    caccGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGAt                                                                             >  2:1915492/1‑150 (MQ=255)

                                                                    caccGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGAt                                                                             >  2:936267/1‑150 (MQ=255)

                                                                       cGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAAATGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGGGTTCGGCAACCTGATGGt                                                                          >  2:4171954/1‑150 (MQ=255)

                                                                                 gCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTc                                                                <  2:1243246/150‑1 (MQ=255)

                                                                                 gCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTc                                                                >  1:3470951/1‑150 (MQ=255)

                                                                                       acacCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGccacc                                                          <  2:3466955/150‑1 (MQ=255)

                                                                                        cacCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGccacca                                                         >  2:4035341/1‑150 (MQ=255)

                                                                                        cacCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGccacca                                                         >  1:356501/1‑150 (MQ=255)

                                                                                          ccGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAAc                                                       <  2:1686578/150‑1 (MQ=255)

                                                                                           cGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACg                                                      <  2:4312069/150‑1 (MQ=255)

                                                                                            gATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGa                                                     <  1:4124576/150‑1 (MQ=255)

                                                                                              ttGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGaaa                                                   <  2:1940415/150‑1 (MQ=255)

                                                                                              ttGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGaaa                                                   <  1:3350170/150‑1 (MQ=255)

                                                                                                gTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGaaaaa                                                 >  1:528531/1‑150 (MQ=255)

                                                                                                    gTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAGCTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGc                                             <  1:266421/150‑1 (MQ=255)

                                                                                                    gTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGc                                             >  1:2218535/1‑150 (MQ=255)

                                                                                                    gTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGc                                             <  1:1230545/150‑1 (MQ=255)

                                                                                                        cGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCAtgt                                         >  1:3636061/1‑150 (MQ=255)

                                                                                                         gAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCAtgtg                                        <  2:1986571/150‑1 (MQ=255)

                                                                                                          aaGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGc                                       >  2:2277767/1‑150 (MQ=255)

                                                                                                          aaGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGc                                       >  2:612227/1‑150 (MQ=255)

                                                                                                          aaGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGc                                       <  1:3976495/150‑1 (MQ=255)

                                                                                                           aGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCg                                      >  2:1736786/1‑150 (MQ=255)

                                                                                                            gttgttACCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGa                                     >  2:848438/1‑150 (MQ=255)

                                                                                                                  aCCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTc                               <  1:1463191/150‑1 (MQ=255)

                                                                                                                  aCCGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTc                               >  1:4317898/1‑150 (MQ=255)

                                                                                                                   ccGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCa                              <  1:3320961/150‑1 (MQ=255)

                                                                                                                   ccGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCa                              >  1:383734/1‑150 (MQ=255)

                                                                                                                    cGGTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCaa                             >  1:27684/1‑150 (MQ=255)

                                                                                                                     ggTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAat                            >  2:2624428/1‑150 (MQ=255)

                                                                                                                      gTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAata                           >  1:1851791/1‑150 (MQ=255)

                                                                                                                      gTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAata                           <  1:3214728/150‑1 (MQ=255)

                                                                                                                      gTTCTTCGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAata                           <  1:3716786/150‑1 (MQ=255)

                                                                                                                        tcttcGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATAtt                         <  2:2557232/150‑1 (MQ=255)

                                                                                                                        tcttcGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATAtt                         <  1:2648615/150‑1 (MQ=255)

                                                                                                                        tcttcGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATAtt                         >  1:4324419/1‑150 (MQ=255)

                                                                                                                        tcttcGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATAtt                         <  1:2029245/150‑1 (MQ=255)

                                                                                                                         cttcGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTg                        >  1:3154755/1‑150 (MQ=255)

                                                                                                                         cttcGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTg                        <  1:2323815/150‑1 (MQ=255)

                                                                                                                         cttcGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTg                        <  1:4237710/150‑1 (MQ=255)

                                                                                                                          ttcGCGGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTAGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGt                       <  1:1571940/150‑1 (MQ=255)

                                                                                                                             gcgGATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTaa                    >  1:1379056/1‑150 (MQ=255)

                                                                                                                               ggATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTaaaa                  >  2:637996/1‑150 (MQ=255)

                                                                                                                                gATGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTaaaaa                 >  2:1486208/1‑150 (MQ=255)

                                                                                                                                  tGAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACg               <  1:464472/150‑1 (MQ=255)

                                                                                                                                  cgAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACg               >  2:2513432/2‑150 (MQ=255)

                                                                                                                                   gAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGc              >  2:3437551/1‑150 (MQ=255)

                                                                                                                                   gAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGc              >  1:809335/1‑150 (MQ=255)

                                                                                                                                   gAAAGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGc              <  1:656145/150‑1 (MQ=255)

                                                                                                                                    aaaGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGcc             >  2:182469/1‑150 (MQ=255)

                                                                                                                                      aGCAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCAc           <  1:1646317/150‑1 (MQ=255)

                                                                                                                                        cAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCg         >  1:133774/1‑150 (MQ=255)

                                                                                                                                        cAGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCg         >  1:1038102/1‑150 (MQ=255)

                                                                                                                                         aGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCgg        <  1:1705062/150‑1 (MQ=255)

                                                                                                                                         aGGTACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCgg        <  2:215801/150‑1 (MQ=255)

                                                                                                                                            tACAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATg     >  1:3630402/1‑150 (MQ=255)

                                                                                                                                             aCAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGt    >  2:4084823/1‑150 (MQ=255)

                                                                                                                                             aCAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGt    <  2:2659092/150‑1 (MQ=255)

                                                                                                                                              cAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTa   >  2:1430492/1‑150 (MQ=255)

                                                                                                                                              cAGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTa   <  2:1069342/150‑1 (MQ=255)

                                                                                                                                               aGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAg  >  2:4282140/1‑150 (MQ=255)

                                                                                                                                               aGCTCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTAAATATTGTTAAAAACGCCACCGGATGTAg  >  2:3307244/1‑150 (MQ=255)

GAATACGCACGCCAGCTCGGCTGCCGCACAGTGGGAATTTCCTGTAATCCGGGGAGCGCCGTTTCAACCACCGCTGAGTTTGCCATTACACCGATTGTAGGTGCCGAAGTTGTTACCGGTTCTTCGCGGATGAAAGCAGGTACAGCGCAGAAACTGGTGCTCAATATGCTTTCCACCGGGCTGATGATTAAATCCGGCAAAGTGTTCGGCAACCTGATGGTCGATGTGGTCGCCACCAACGAAAAACTGCATGTGCGACAGGTCAATATTGTTAAAAACGCCACCGGATGTAG  >  AP012306/2098643‑2098935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`