breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 29 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG37.5% 524.5 / 69.2 292intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123063,938,8870CT34.4% 335.2 / 184.1 224S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP012306341,3770TG32.8% 211.7 / 18.3 129G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP012306644,4170GA31.1% 828.5 / inf 557intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123061,944,2880AC30.4% 472.5 / 51.7 224G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP012306935,0540GT29.3% 202.7 / 41.7 82intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP0123063,182,8610AC29.0% 285.1 / 31.2 131L198F (TTA→TTCyiaNpredicted transporter
*AP0123063,020,6700TG28.7% 343.9 / 12.2 181K195N (AAA→AACglgCglucose‑1‑phosphate adenylyltransferase
*AP0123063,527,3680AC27.4% 613.6 / 58.0 253L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP012306800,6750AC26.9% 541.5 / 39.8 245T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123061,181,3690AC26.6% 351.4 / 27.2 143K103N (AAA→AACycjSpredicted oxidoreductase, NADH‑binding
*AP0123061,226,5570AG26.1% 392.8 / 74.5 180G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123063,903,7910AG25.8% 523.1 / 68.4 233E189G (GAA→GGA) yjgJpredicted transcriptional regulator
*AP0123061,854,8510AC25.7% 733.7 / 87.3 319T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123062,558,0710AC25.7% 593.9 / 34.2 249V302G (GTA→GGA) visCpredicted oxidoreductase, FAD/NAD(P)‑binding domain
*AP0123062,680,3450AC24.4% 445.2 / 44.7 214T184P (ACC→CCC) ttdAL‑tartrate dehydratase, alpha subunit
*AP0123063,012,8850AC23.2% 545.6 / 17.3 224V165G (GTG→GGG) glpGpredicted intramembrane serine protease
*AP0123062,212,7530AC22.8% 389.2 / 25.7 149Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
*AP0123063,590,3670AC22.4% 657.7 / 22.1 246T30P (ACC→CCC) fabRDNA‑binding transcriptional repressor
*AP0123061,393,3790AC21.8% 749.2 / 40.6 284T92P (ACC→CCC) nthDNA glycosylase and apyrimidinic (AP) lyase

Marginal new junction evidence (lowest skew 10 of 222 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 215364NA (NA)18 (0.060) 17/288 14.6 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 = 3600650NA (NA)60 (0.200)
+AAAGG
13/288 16.0 NA noncoding (2797/2904 nt) rrlB 23S ribosomal RNA (rrlB)
?AP012306 = 3642048 NA (NA)noncoding (2793/2904 nt) rrlE 23S ribosomal RNA (rrlE)
* ? AP012306 = 3600658NA (NA)34 (0.110) 13/292 16.1 NA noncoding (2805/2904 nt) rrlB 23S ribosomal RNA (rrlB)
?AP012306 = 3642045 NA (NA)noncoding (2790/2904 nt) rrlE 23S ribosomal RNA (rrlE)
* ? AP012306 = 997500316 (1.000)12 (0.040) 12/294 16.5 3.8% coding (44/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997996 = 289 (0.920)coding (540/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 = 1092512401 (1.260)13 (0.050) 8/258 17.3 4.0% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 283 (1.030)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 1092334366 (1.150)9 (0.030) 8/258 17.3 2.9% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 283 (1.030)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 213952 =NA (NA)10 (0.030) 10/298 17.4 NA noncoding (1321/1542 nt) rrsH 16S ribosomal RNA (rrsH)
?AP012306 213970 = NA (NA)noncoding (1339/1542 nt) rrsH 16S ribosomal RNA (rrsH)
* ? AP012306 3372707 =NA (NA)9 (0.030) 8/274 17.7 NA noncoding (60/76 nt) gltU tRNA‑Glu
?AP012306 3372719 = NA (NA)noncoding (72/76 nt) gltU tRNA‑Glu
* ? AP012306 = 152555316 (1.000)11 (0.040) 9/292 17.7 3.5% coding (3/480 nt)
coding (1397/1398 nt)
folK
pcnB
2‑amino‑4‑hydroxy‑6‑hydroxymethyldihyropteridine pyrophosphokinase
poly(A) polymerase I
?AP012306 = 152570 305 (0.980)coding (1382/1398 nt) pcnB poly(A) polymerase I
* ? AP012306 3597694 =343 (1.080)8 (0.030) 8/276 17.8 2.4% intergenic (+34/‑160) gltT/rrlB tRNA‑Glu/23S ribosomal RNA (rrlB)
?AP012306 3597734 = 327 (1.110)intergenic (+74/‑120) gltT/rrlB tRNA‑Glu/23S ribosomal RNA (rrlB)