Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306341,3770TG38.4% 192.8 / 37.1 133G60G (GGT→GGG) ribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
Reads supporting (aligned to +/- strand):  ref base T (26/56);  new base G (50/1);  total (76/57)
Fisher's exact test for biased strand distribution p-value = 7.11e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 197 total aligned reads displayed.

CTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCG  >  AP012306/341228‑341526
                                                                                                                                                      |                                                                                                                                                     
  aaaGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGa                                                                                                                                                     <  1:3153745/150‑1 (MQ=255)
   aaGCTGGCGCAACGAGGACGTTTTGCCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGaa                                                                                                                                                    <  1:1975561/150‑1 (MQ=255)
   aaGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGaa                                                                                                                                                    <  1:1680666/150‑1 (MQ=255)
        ggCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGc                                                                                                                                               <  1:3032094/150‑1 (MQ=255)
        ggCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGc                                                                                                                                               <  2:4083138/150‑1 (MQ=255)
        ggCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGGGCGGCATTGGCAAAGATGGCGAAATTGTCGGGGAAGGTTACCACCAACGGGCGGGTGAACCACAAGCCGAAGTACACGCCTTGCGAATGGC‑GGGGGgaaaaaa                                                                                                                                               >  2:2063197/1‑148 (MQ=255)
          cgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGgaaaaaaca                                                                                                                                             >  2:1581131/1‑146 (MQ=255)
            cAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCaaa                                                                                                                                           <  1:1753846/150‑1 (MQ=255)
             aaCGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACGAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAg                                                                                                                                          <  2:3660518/150‑1 (MQ=255)
               cGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGt                                                                                                                                        <  2:3464363/150‑1 (MQ=255)
                gAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGGAAAAACCAAAggg                                                                                                                                        >  2:2981716/1‑149 (MQ=255)
                gAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCaaaaggg                                                                                                                                       >  1:3462749/1‑146 (MQ=255)
                 aGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAACCCAAAgagc                                                                                                                                       >  1:2275585/1‑147 (MQ=255)
                    aCGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGAc                                                                                                                                   <  1:486783/150‑1 (MQ=255)
                      gTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCg                                                                                                                                 <  1:4249044/150‑1 (MQ=255)
                      gTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAAGGGCGACCg                                                                                                                                 >  1:2672681/1‑150 (MQ=255)
                        tttACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGGGACcccc                                                                                                                               >  2:3988093/1‑150 (MQ=255)
                          tACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTa                                                                                                                             <  2:2770477/150‑1 (MQ=255)
                          tACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTa                                                                                                                             <  2:2530594/150‑1 (MQ=255)
                            ccACGCATCCCACCCCGAATGTCGGGTGCGTCATTGTCAAAGAGGGCGAAATTGTCGGTGAAGGTTACCACCAACGGGCGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTAGGGC‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATg                                                                                                                           <  2:2918401/150‑1 (MQ=255)
                              aCGCACCCCACCCCGAATGTGGGGTGCGTCATTGTAAAAGATGGCGAAATTGTCGGTGAAGGTTACCCCAAACGTGCGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTAGGGG‑GGGTAAAAAACCCAAAGGTGCGACCGCCTATGTc                                                                                                                         <  2:2275886/150‑1 (MQ=255)
                                  aTCCCACCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTCCCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCacac                                                                                                                     <  1:3597103/150‑1 (MQ=255)
                                  aTCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAAGTGCGACCGCCTATTTCacac                                                                                                                     >  1:2642045/1‑150 (MQ=255)
                                  aTCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAAACAAAAGGGCGGCCCCCCCTGTCacac                                                                                                                     >  2:3150351/1‑150 (MQ=255)
                                   tCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGG‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACt                                                                                                                    <  1:4025759/150‑1 (MQ=255)
                                    cccAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCGACCGCCTATGTTCCACTc                                                                                                                   >  1:2258217/1‑150 (MQ=255)
                                       aaCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAAACAAAAGGGGGACCGCCCATGTCacaccccca                                                                                                                >  2:1108638/1‑145 (MQ=255)
                                         cccGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGTCTATGTCACACTCGAAcc                                                                                                              <  2:1767549/150‑1 (MQ=255)
                                          ccGAATGTCGGGTGCGTCATTGTAAAAGAGGGGAAAATTGGCGGTGAGGTTTACCACAAACGGGCGGGTAAACCACAGGCCGAAGAACACGCGTTGCGAAGGGG‑GGGAAAAAAAGCAAAAGGTGCAACCGCCTATGTCACACTCGAAccc                                                                                                             <  2:1996809/150‑1 (MQ=255)
                                               tGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAGGAGCGACCGCCCATGGTACACCCGAACCCCGTAg                                                                                                        >  1:281020/1‑150 (MQ=255)
                                                 tCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAAACAAAAGGGGGGCCGCCCCTTTCTCACACCAACCCCGTAGcc                                                                                                      >  2:971082/1‑150 (MQ=255)
                                                  cGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAAACAAAAGGGCGGCCCCCTATGTCACACTCGAACCCTGTAGCca                                                                                                     >  2:754854/1‑150 (MQ=255)
                                                  cGGGTGCGTAATTGACAAAGATGGCGAAATTGTCGGTGAAGTTTACCACCAAGGTGCGGGTGACCCACATGCCGAAGTACACGCGTTGCGTAGGGG‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCca                                                                                                     <  2:801084/150‑1 (MQ=255)
                                                    ggTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGGGACCGCCCATGTCACACTCCAACCCCGTAGCcatc                                                                                                   >  2:173033/1‑150 (MQ=255)
                                                     gTGCGTCATTGTAAAAGAGGGCAAAATTGTGGGTGAAGTTTCCCACCAACGTGGGGGTAACCCACATGCCGAAGTACACGCGTTGCGTAGGGG‑GGGTAAAAAAGCAAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCcatca                                                                                                  <  2:949356/150‑1 (MQ=255)
                                                        cGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGGGGCCGCCTATGGTACACTCGAAACCCGTAGCCAACAAgg                                                                                               >  1:746552/1‑150 (MQ=255)
                                                           cATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑TGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCg                                                                                            >  1:2699727/1‑150 (MQ=255)
                                                           cATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAAACCTGTAGCCATCATGGTCg                                                                                            >  1:3728167/1‑150 (MQ=255)
                                                           cATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAAGAGCGACCGCCCATGTCACACTCGAACCCTGTAGCCATCATGGTCg                                                                                            >  1:1475972/1‑150 (MQ=255)
                                                            aTTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGGAAAAACCAAAAGTGCGACCCCCTCTGTCACACTCGAACCCCGTAGCCATCATGGTCGt                                                                                           >  1:2076270/1‑150 (MQ=255)
                                                            aTTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGGGACCCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGt                                                                                           <  1:3334648/150‑1 (MQ=255)
                                                            aTTGTAAAAGATGGCGAAATTGTGGGTGAAGGTTACCACCAACGTGGGGGTGAACCACATGCCGAAGTACCCGCGTTGCGTATGGC‑GGGTGAAAAAGCAAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGt                                                                                           <  2:636148/150‑1 (MQ=255)
                                                              tGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAAGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTAc                                                                                         >  1:1231372/1‑150 (MQ=255)
                                                               gTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGGAAAAGCCAAAGGTGCGGCCGCCTATGTCACACTCCAACCCTGTAGGCCTAATAGTCGTACg                                                                                        >  2:1408765/1‑150 (MQ=255)
                                                               gTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACg                                                                                        <  1:236593/150‑1 (MQ=255)
                                                                 cAAAGATGGCGAAATTGTCGGTGAAGGTTCCCACCAACGTGCGGGTGACCCACATGCCGAAGTCCCCGCGTGGCGTATGGC‑GGGGGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGcc                                                                                      <  2:2807913/150‑1 (MQ=255)
                                                                 cAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGGGACCGCCTATTTCACCCTCCAACCCCGTAGCCCTCATGGGGGTACGcc                                                                                      >  2:2895011/1‑150 (MQ=255)
                                                                 cAAAGAGGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGG‑GGGTAAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGcc                                                                                      <  2:3421497/150‑1 (MQ=255)
                                                                     gATGGCGAATTTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCg                                                                                  <  1:3991145/150‑1 (MQ=255)
                                                                      aTGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGt                                                                                 <  2:1624916/150‑1 (MQ=255)
                                                                       tGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGGAAAAACCAAAAGTGCGGCCGCCCATGTCACACCCCGACCCCGTAGCCCTCATGGGCGTACGCCCCCCTg                                                                                >  2:2594250/1‑150 (MQ=255)
                                                                       tGGAGAACTTGTGGGTGAAGGTTCCCACCAACGTGCGGGTAAACCACATGCCAAAGTACACGCGTTGGGTATGGG‑GGGTGAAAAAGCAAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTg                                                                                <  2:238802/150‑1 (MQ=255)
                                                                        ggCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGc                                                                               <  1:2615956/150‑1 (MQ=255)
                                                                          gaaaaTGGTGGGTGAGGTTTCCCACCAAGGTGGGGGTAACCCACATGCGAAAGTACACGCGTGGCGTAGGGG‑GGGTAAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCtg                                                                             <  2:928337/148‑1 (MQ=255)
                                                                          caaaaTTGTCGGGGAAGTTTCCCACAAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTGGCGTATGGC‑GGGGGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCtg                                                                             <  2:3534142/148‑1 (MQ=255)
                                                                          cGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTAGGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCtg                                                                             <  1:1156580/150‑1 (MQ=255)
                                                                           aaatttGTGGGAGAGGTTTACCACCAACGGGCGGGTGAACCACATGCGGAAGTACACGCTTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGTGCGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCtgt                                                                            <  2:4117483/146‑1 (MQ=255)
                                                                             aaTTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTCTAGCCATCCTGGTCGTACGCCACCGGGCTGTGa                                                                          >  2:38083/1‑150 (MQ=255)
                                                                              aTTGTCGGTGAAGGTTCCCACCAACGTGCGGGTGAACCACATCCCGAAGTACCCGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGAc                                                                         <  2:2341920/150‑1 (MQ=255)
                                                                                 gTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGCGCGGCCGCCGATGTCACACCCCAACCCCGTAGGCCTCATGGTCGGACGCCACCCGGCTGTGAAGCa                                                                      >  2:2957438/1‑150 (MQ=255)
                                                                                  tCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAGGGGCGACCGCCCATTTCACACTCGAACCCTGTGGCCATCATGGGCGTACGCCACCCTGCTGGGGCGCAc                                                                     >  2:1819156/1‑150 (MQ=255)
                                                                                       gAAGGTTCCCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATc                                                                <  1:1515063/150‑1 (MQ=255)
                                                                                       gAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAAGGGCGACCGCCTATGTCCCACTCCAACCCCGTAGCCATCATGGTCGTACGCCCCCGCGCTGTGACGCActcctc                                                                >  2:368274/1‑150 (MQ=255)
                                                                                           gTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAAGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATcgccg                                                            >  1:1463810/1‑150 (MQ=255)
                                                                                                 accaACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAAGGGCGACCGCCTATTTCACACCCGAACCCTGTAGCCCCCATGGGCGTACGCCACCCTGCTGTGACGCACCCCTCCCCCCTGGCg                                                      >  2:2852019/1‑150 (MQ=255)
                                                                                                  ccaACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGCGACCGCCCATGTCACACTCGAAACCTGTAGCCATCATGGGCGTACGCCACCGTGCTGTGACGCACCCATCGCCGCCGGCGt                                                     >  2:3588572/1‑150 (MQ=255)
                                                                                                     aggTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTAGGGG‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAgc                                                  <  1:1265724/148‑1 (MQ=255)
                                                                                                      cGTGGGGGTGAACCACATGCGGAAGTACACGCGTTGCGTAGGGG‑GGGTAAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAgcg                                                 <  2:586523/150‑1 (MQ=255)
                                                                                                       gTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGCCAAAAGGGCGGCCGCCCATTTTCCCCTCGAACCCCGTAGGCATCATTGGCGTACGCCCCCCTGCTGGGACGGACCCATCGCCGCCGGCGGAgccc                                                >  2:2262247/1‑148 (MQ=255)
                                                                                                         gCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAAGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAgcgcgc                                              >  1:649690/1‑150 (MQ=255)
                                                                                                          cGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCCAAGGGGCGACCGCCTCTGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCCGGCTGTGACGCACTCACCCCCGCTGGCGGAGcgcgcg                                             >  2:3313995/1‑150 (MQ=255)
                                                                                                           gggTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGGCAAAAGGGGGGCCGCCCATGTCACACTCGAACCCCGTAGGCATCATGGGCGGACGCCACCCGGCTGTGAGGCACTCATCGCCGCCGGCGGAGcgcgcgg                                            >  2:563840/1‑149 (MQ=255)
                                                                                                              taaaCCACATGCCGAAGTACCCGCGTTGCGTATGGC‑GGGTGAAAAAGCAAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGt                                         <  2:2121372/148‑1 (MQ=255)
                                                                                                              tGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGt                                         <  1:3306334/150‑1 (MQ=255)
                                                                                                                 aCCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCGACCGCCCATGTCACACTCGAACCCTGTAGCCATCATGGGCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCCGGCGGAGCGCGCGTGGGTGc                                      >  2:616861/1‑150 (MQ=255)
                                                                                                                     caTGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGGGACCGCCCCTGTCACCCTCGAACCCTGTAGCCATCATGGTCGTACGCCCCCGTGCTGTGACGCACTCCTCGCCGCCGGCGGAGCGCGCGGGGTTGGCTCg                                  >  2:3916165/1‑150 (MQ=255)
                                                                                                                          cGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAGGGGCGACCCCCTATGTCACCCCCCAACCCCGTAGCCATCATGGTCGTACGCCACCGGGCTGTGACGCACCCCCCGCCGCTGGCGGAGCGCGCGTGGTTGGCTCGATGCa                             >  2:3504045/1‑150 (MQ=255)
                                                                                                                           gAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCGACCCCCCATTTCACACTCGAACCCTGTAGCCACCATGGTCGTACGCCCCCGTGCTGTGACGCACTCATCGCCGCCGGCGGAGCGCGCGGGGTTGCCTCGGTGCaa                            >  2:3385884/1‑150 (MQ=255)
                                                                                                                            aaGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAAGGGCGACCGCCCATGTCACACCCGAACCCTGTAGCCATCATGGTCGTACGCCCCCGTGCTGTGACGCACTCATCGCCCCTGGCGGAGCGCGCGGGGGTGGCTCGATGCAAg                           >  2:4216660/1‑150 (MQ=255)
                                                                                                                            aaGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGGGACCGCCCATGTCACACTCGAACCCCGTAGCCATCATGGGCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAg                           >  2:2164802/1‑150 (MQ=255)
                                                                                                                             aGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCGACCGCCCATGTCACACTCGAACCCCGTAGCCATCATGGTCGTACGCCCCCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGGTGCCTCGATGCAAGa                          >  2:3499603/1‑150 (MQ=255)
                                                                                                                              gTACACGCGTTGCGTATGGC‑GGGTGAAAAACCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGAt                         <  2:1195978/150‑1 (MQ=255)
                                                                                                                               tACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGGGACCCCCCATGTCCCACCCGAACCCCGTAGCCAACATGGGCGGACGCCCCCGTGCTTTGAGGCACTCATCGCCCCTGGGGGAGCGCGCGGGGGTGGCTCGATGCAAGATc                        >  2:2464581/1‑150 (MQ=255)
                                                                                                                               tACACGCGTGGCGTAGGGG‑GGGTAAAAAAGCAAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATc                        <  1:2539214/150‑1 (MQ=255)
                                                                                                                                ccccgctTTGCGTATGGC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATcc                       <  1:2858811/147‑1 (MQ=255)
                                                                                                                                acccgctTTGCGTATGGG‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATcc                       <  1:537233/147‑1 (MQ=255)
                                                                                                                                 cccgcgTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCt                      <  1:195356/148‑1 (MQ=255)
                                                                                                                                 cacGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCGACCCCCCATTTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCCCCGCCGGCGTAGCGCGCGTGGGTGCCTCCCTGCAAGATCCt                      >  2:1664237/1‑150 (MQ=255)
                                                                                                                                     cgTGGCGTATGGC‑GGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAAcc                  <  1:896469/150‑1 (MQ=255)
                                                                                                                                          cGTAGGGG‑GGGTAAAAAGGCAAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAg             <  2:3154497/150‑1 (MQ=255)
                                                                                                                                           gTATGGC‑GGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGGCGTACGCCACCGTGCTGTGACGCACCCATCGCCGCTGGCGTAGCGGGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAgg            >  2:506240/1‑150 (MQ=255)
                                                                                                                                           gTATGGC‑GGGGGAAAAAGCCAAAAGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAgg            >  2:3060627/1‑150 (MQ=255)
                                                                                                                                             tagggggggTAAAAAGGCAAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGt           <  1:3833832/144‑1 (MQ=255)
                                                                                                                                              tGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCg         >  2:2925581/1‑150 (MQ=255)
                                                                                                                                              tGGC‑GGGTGAAAAAGCAAAAGATGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCg         <  1:3399105/150‑1 (MQ=255)
                                                                                                                                                 c‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTg      >  2:1333060/1‑150 (MQ=255)
                                                                                                                                                   gggTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTgg     >  2:3206832/1‑150 (MQ=255)
                                                                                                                                                   gggTGAAAAAGCAAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTgg     <  1:1055244/150‑1 (MQ=255)
                                                                                                                                                    ggTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTggg    <  1:135414/150‑1 (MQ=255)
                                                                                                                                                     gTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGc   <  1:1581131/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCG  >  AP012306/341228‑341526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑