Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,181,3690AC27.6% 278.8 / 28.3 116K103N (AAA→AAC) ycjSpredicted oxidoreductase, NADH‑binding
Reads supporting (aligned to +/- strand):  ref base A (76/8);  new base C (0/32);  total (76/40)
Fisher's exact test for biased strand distribution p-value = 1.98e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 163 total aligned reads displayed.

AATACGGGAATGCATCCGTGTGGGACGATCCGCAGGCCATGCTGCTGGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACGGCGTTTTGGGAGAGAT  >  AP012306/1181221‑1181518
                                                                                                                                                     |                                                                                                                                                     
aaTACGGGAATGCATCCGTGTGGGACGATCCGCAGGCCATGCTGCTGGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAAc                                                                                                                                                      <  2:102005/150‑1 (MQ=255)
aaTACGGGAATGCATCCGTGTGGGACGATCCGCAGGCCATGCTGCTGGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAAc                                                                                                                                                      <  1:512644/150‑1 (MQ=255)
     gggAATGCATCCGTGTGGGACGATCCGCAGGCCATGCTGCTGGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAAccgccc                                                                                                                                                 >  1:1569809/1‑150 (MQ=255)
      ggAATGCATCCGTGTGGGACGATCCGCAGGCCATGCTGCTGGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAAccgcccg                                                                                                                                                <  1:3507879/150‑1 (MQ=255)
         aTGCATCCGTGTGGGACGATCCGCAGGCCATGCTGCTGGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCa                                                                                                                                             <  2:3253336/150‑1 (MQ=255)
           gCATCCGTGTGGGACGATCCGCAGGCCATGCTGCTGGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATg                                                                                                                                           >  2:2948119/1‑150 (MQ=255)
               ccGTGTGGGACGATCCGCAGGCCATGCTGCTGGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATAGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGc                                                                                                                                       >  1:2753577/1‑150 (MQ=255)
                 gtgtGGGACGATCCGCAGGCCATGCTGCTGGCGGTGAAAACTGGTGGGGGTAGCGTCCTCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGGACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAACCCGCCCGCCATGACGCCa                                                                                                                                     >  2:3701443/1‑150 (MQ=255)
                   gggggACGCCCCGCGGGCCATGCTGGGGGGGGTAAACCCTGATGTGGTTGGCGTCTGCCCCCCTACCCTTTTTCATTACAAACATCCCCTGAGGGCACGGGAGGG‑GGGCTGCAATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGa                                                                                                                                   <  2:294401/148‑1 (MQ=255)
                    tgGGACGATCCGCAGGCCATGCTGCTGGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGGAAAACCGCCCGCCATGACGCCAGaa                                                                                                                                  >  1:310614/1‑150 (MQ=255)
                      ggACGACCCGCAGGCCATGCTGCGGGCGGTAAAACTTGATGTGGTTAGCGTCTGCCCACCTACCCTTTTTCATTACAAACATCCCCTGATGGCACTGGAGGG‑GGGCTGCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACa                                                                                                                                <  2:2496090/150‑1 (MQ=255)
                        aCGATCCGCAGGCCATGCTGCTGGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCCCCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAgg                                                                                                                              >  2:2238773/1‑150 (MQ=255)
                                         ctgctgGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCCTGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAAGCGCGGGAAATGTGCGAt                                                                                                             >  1:897882/1‑150 (MQ=255)
                                         ctgctgGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGGAATGTGCGAt                                                                                                             >  2:1184538/1‑150 (MQ=255)
                                         ctgctgGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGAt                                                                                                             >  1:2634987/1‑150 (MQ=255)
                                         ctgctgGCGGTGAAAACTGATGTGGTTAGCGTCTGGTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAAGCGCGGGAAAtgggggaa                                                                                                             >  2:2915420/1‑144 (MQ=255)
                                         ctgcggGCGGTAAACCTTGTTGTGGTTAGCTTCTCCCCCCCTACCCTTTTTCATTACAACCATCCCCTGATGCCCCTGGAAGC‑GTCCTCCCTTGTGTTGTGCAAAAACCCCCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGAt                                                                                                             <  2:1900728/150‑1 (MQ=255)
                                          tgctgGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATa                                                                                                            >  1:162178/1‑150 (MQ=255)
                                            ctgGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACATGCGCGGGAAATGTGCGATAcc                                                                                                          >  1:380416/1‑150 (MQ=255)
                                            ctgGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATAcc                                                                                                          >  1:128072/1‑150 (MQ=255)
                                                    gAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCaa                                                                                                  >  2:627704/1‑150 (MQ=255)
                                                      aaCCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAAc                                                                                                >  1:483834/1‑150 (MQ=255)
                                                      aaCCTGATGTGGTTAGCGTCTGCCCACCTAACCGTTTTCATTACGAACATCCCCTGAGGGCACGGGAGGC‑GGGCTGCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAAc                                                                                                <  2:2297962/150‑1 (MQ=255)
                                                       accttgtgtgGTGGGTGTCGGCTCCCTTAACCGTTTTCATTACGAACATCCCTTGAGGGCTGGGGAAGG‑GGGCTGCCATGTGATGGGAAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACt                                                                                               <  2:1433965/144‑1 (MQ=255)
                                                        ccTGATGTGGTTAGCGTCTGCTCACCTACCCGTTTTCATTACGAACATCCCCTGAGGGCACGGGAGGC‑GGGCTGCCATGGGATGTGCGAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTg                                                                                              <  2:618664/150‑1 (MQ=255)
                                                           gATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGc                                                                                           >  2:2798402/1‑150 (MQ=255)
                                                            aTGTGGTTAGCTTCTGCCCACCTACCTGTTTTCATTACGAACATCCCCTGATGGCACGGGAGGG‑GGGCGGCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCa                                                                                          <  2:1165357/150‑1 (MQ=255)
                                                            aTGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCa                                                                                          >  2:1990480/1‑150 (MQ=255)
                                                            aTGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCa                                                                                          >  1:2902947/1‑150 (MQ=255)
                                                             tgtgGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCaa                                                                                         >  1:2903161/1‑150 (MQ=255)
                                                             tgtgGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCaa                                                                                         >  1:3995521/1‑150 (MQ=255)
                                                                    aGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTg                                                                                  >  2:3713447/1‑150 (MQ=255)
                                                                         cTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTa                                                                             >  1:4105328/1‑150 (MQ=255)
                                                                         cTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTa                                                                             >  1:761636/1‑150 (MQ=255)
                                                                            cTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGa                                                                          >  2:3409066/1‑150 (MQ=255)
                                                                            cTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGa                                                                          >  1:3508975/1‑150 (MQ=255)
                                                                               aCCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACtt                                                                       >  2:2798862/1‑150 (MQ=255)
                                                                               aCCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGGGCTGGCCTACGACtt                                                                       >  2:3905760/1‑150 (MQ=255)
                                                                                ccTAACCTTTTTCATTACGAACATCCCCTGAGGGCACGGGAAGC‑GGGCTGCTATGTGATGTGCGAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACttt                                                                      <  2:1641021/150‑1 (MQ=255)
                                                                                ccTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGCCGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACttt                                                                      >  2:1670743/1‑150 (MQ=255)
                                                                                 cTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTc                                                                     >  1:434644/1‑150 (MQ=255)
                                                                                  taccctttttCATTACAAACATCCCTTGATGGCACGGGAGGC‑GGGCTGCTATGTGTTGTGAAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCa                                                                    <  2:4247809/144‑1 (MQ=255)
                                                                                   aaCCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCAc                                                                   >  1:1873788/1‑150 (MQ=255)
                                                                                   aaCCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCAc                                                                   >  1:485971/1‑150 (MQ=255)
                                                                                     ccGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCa                                                                 >  1:2170714/1‑150 (MQ=255)
                                                                                      tgTTTTCATTAGAAACATCCCCTGA‑GGCCCCGGGAGCGGGGCTGCAATGGGGTGGGGAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCAt                                                                <  2:1298397/149‑1 (MQ=255)
                                                                                        ttttCATTACGAACATCCCCTGAGGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCg                                                              <  2:2671809/150‑1 (MQ=255)
                                                                                          tccATTACAAACATCCCTGGAGGGCACGGGAGGC‑GGGCTGCCATGTGTTGTGCGAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGtt                                                            <  1:573889/148‑1 (MQ=255)
                                                                                            cATTACAAACATCCCCTGATGGCACTGGAGGC‑GGGCTGCCATGTGATGTGAAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGtttt                                                          <  2:162178/150‑1 (MQ=255)
                                                                                             aTTACGAACATCCCCTGAGGGCACGGGAGGC‑GGGCTGCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTg                                                         <  1:1813686/150‑1 (MQ=255)
                                                                                             aTTACAAACATCCCCTGAGGGCAGGGGAGGG‑GGGCTGCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTg                                                         <  2:2687247/150‑1 (MQ=255)
                                                                                              ttACGAACATCCCCTGAGGGCACGGGAGGC‑GGGCGGCCATGTGATGTGAAAAAAACGGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTgc                                                        <  1:1926776/150‑1 (MQ=255)
                                                                                                 cGAACATCCCCTGATGGCACGGGAGGG‑GGGCTGCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCt                                                     <  1:1762408/150‑1 (MQ=255)
                                                                                                 cGAACATCCCCTGATGGCACGGGAGGC‑GGGCTGCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCt                                                     <  1:1184538/150‑1 (MQ=255)
                                                                                                 cGAACATCCCCTGAGGGCATGGGAAGC‑GGGCTGCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCt                                                     <  1:1515978/150‑1 (MQ=255)
                                                                                                  gAACATCCCCTGATGGCACGGGGGGC‑GGGCCGCGTGGGGAGGGGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTc                                                    <  2:824871/150‑1 (MQ=255)
                                                                                                  gAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTc                                                    >  1:399801/1‑150 (MQ=255)
                                                                                                    aaatccccTGAGGGCACTGGAGGG‑GGGCTGCGTGGTGATGTGAAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGa                                                  <  2:766974/145‑1 (MQ=255)
                                                                                                     cATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGAt                                                 <  1:2279872/150‑1 (MQ=255)
                                                                                                       tccccGGATGCCACGGGAGGG‑GGGCTGCAATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATAc                                               <  2:212042/148‑1 (MQ=255)
                                                                                                         cccTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGc                                             >  2:3973036/1‑150 (MQ=255)
                                                                                                         cccTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGc                                             >  2:3493038/1‑150 (MQ=255)
                                                                                                           cTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCaa                                           >  2:1832934/1‑150 (MQ=255)
                                                                                                            tGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAAc                                          >  2:971160/1‑150 (MQ=255)
                                                                                                            ggATGGCACTGGAGGG‑GGGCTGCCATGTGATGTGAAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAAc                                          <  2:2333223/149‑1 (MQ=255)
                                                                                                              aTGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAg                                        >  1:1333566/1‑150 (MQ=255)
                                                                                                               tGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGc                                       >  2:496375/1‑150 (MQ=255)
                                                                                                                gccgcTGGAGGG‑GGGCTCCGGTGTGATGTGAAAAACCCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCt                                      <  2:2343938/146‑1 (MQ=255)
                                                                                                                 gccggggAGGC‑GGGCTGCCATGTGATGGGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTg                                     <  2:661431/145‑1 (MQ=255)
                                                                                                                 gCACGGGAGGG‑GGGCTGCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTg                                     <  2:1165460/150‑1 (MQ=255)
                                                                                                                  catgggAGGC‑GGGCTGCCATGTGATGTGAAAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGc                                    <  1:823724/146‑1 (MQ=255)
                                                                                                                   agtGGAGGC‑GGGCTGCCATGTGATGTGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCg                                   <  1:2072336/148‑1 (MQ=255)
                                                                                                                   aCTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCg                                   >  2:3096590/1‑150 (MQ=255)
                                                                                                                    cgggAGGC‑GGGCTGCCATGTGAGGTGAAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGt                                  <  2:3972159/148‑1 (MQ=255)
                                                                                                                    cgggAGGC‑GGGCTGCATTGGGATGTGCGAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGt                                  <  2:1618938/148‑1 (MQ=255)
                                                                                                                    cTGGAGGC‑GGGCTGCGGTGTGAGGTGAAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGt                                  <  1:242528/150‑1 (MQ=255)
                                                                                                                     tgcaggC‑GGTCGGCCAGGGGTTGTGAAAAAACCGGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTg                                 <  2:348291/145‑1 (MQ=255)
                                                                                                                          gC‑GGGCTGCCATGTGATGGGCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAg                            <  2:1424096/150‑1 (MQ=255)
                                                                                                                           c‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAgg                           >  2:1013676/1‑150 (MQ=255)
                                                                                                                           c‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAgg                           >  2:308848/1‑150 (MQ=255)
                                                                                                                             gggCTGCCGTGTGATGTGCAAAAACCCCCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGt                          <  2:1966985/150‑1 (MQ=255)
                                                                                                                              ggCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTg                         >  2:2569030/1‑150 (MQ=255)
                                                                                                                               gCTGCAATGTGATGCGAAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGa                        <  1:1566367/150‑1 (MQ=255)
                                                                                                                                cTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGAc                       >  1:2447810/1‑150 (MQ=255)
                                                                                                                                cTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGAc                       >  1:2929411/1‑150 (MQ=255)
                                                                                                                                cTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGAc                       <  1:2948119/150‑1 (MQ=255)
                                                                                                                                 tGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGAcc                      >  2:319600/1‑150 (MQ=255)
                                                                                                                                  gCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCa                     >  1:2732780/1‑150 (MQ=255)
                                                                                                                                    cATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAAc                   >  2:2050086/1‑150 (MQ=255)
                                                                                                                                     ttgtgatgggCAAAAACCCCCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCACCg                  <  2:2977648/149‑1 (MQ=255)
                                                                                                                                      tgtgatgtgCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACgg                 <  2:3261944/150‑1 (MQ=255)
                                                                                                                                      ggtgatgggCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACgg                 <  1:3179319/149‑1 (MQ=255)
                                                                                                                                         gatgtgCAAAAACCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACGGCGt              <  1:2220176/150‑1 (MQ=255)
                                                                                                                                          atgtgCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACGGCGtt             >  1:338917/1‑150 (MQ=255)
                                                                                                                                            gtgCAAAAACCCCCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACGGCGtttt           <  2:4154989/150‑1 (MQ=255)
                                                                                                                                              gcaaaaacCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACGGCGTTTTgg         <  1:4214523/147‑1 (MQ=255)
                                                                                                                                               caaaaacCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACGGCGTTTTggg        <  1:3358535/148‑1 (MQ=255)
                                                                                                                                               cGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACGGCGTTTTggg        >  2:1047470/1‑150 (MQ=255)
                                                                                                                                                 aaaaaCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACGGCGTTTTGGgag      >  1:66704/1‑150 (MQ=255)
                                                                                                                                                   aaaCCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACGGCGTTTTGGgagag    >  1:1017998/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AATACGGGAATGCATCCGTGTGGGACGATCCGCAGGCCATGCTGCTGGCGGTGAAACCTGATGTGGTTAGCGTCTGCTCACCTAACCGTTTTCATTACGAACATACCCTGATGGCACTGGAAGC‑GGGCTGCCATGTGATGTGCGAAAAACCGCCCGCCATGACGCCAGAACAGGCGCGGGAAATGTGCGATACCGCGCGCAAACTGGGCAAGGTGCTGGCCTACGACTTTCACCATCGTTTTGCGCTCGATACGCAACAGCTGCGTGAACAGGTGACCAACGGCGTTTTGGGAGAGAT  >  AP012306/1181221‑1181518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑