Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,558,0710AC22.8% 592.5 / 21.8 233V302G (GTA→GGA) visCpredicted oxidoreductase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base A (118/62);  new base C (0/53);  total (118/115)
Fisher's exact test for biased strand distribution p-value = 1.93e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 267 total aligned reads displayed.

CTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAA  >  AP012306/2557923‑2558220
                                                                                                                                                    |                                                                                                                                                     
cTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTAc                                                                                                                                                      >  1:2322393/1‑150 (MQ=255)
 tGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTAcc                                                                                                                                                     <  1:810123/150‑1 (MQ=255)
  gCATCCCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTAccc                                                                                                                                                    <  2:1028467/150‑1 (MQ=255)
   cATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTAcccc                                                                                                                                                   <  1:451826/150‑1 (MQ=255)
    aTACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTAccccc                                                                                                                                                  >  2:808320/1‑150 (MQ=255)
      aCCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCACCATCCATAAAGCCGAGATTTACCCCCTg                                                                                                                                                <  2:1629330/150‑1 (MQ=255)
       ccagccagCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGc                                                                                                                                               >  2:789509/1‑150 (MQ=255)
         agccagcATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGccc                                                                                                                                             <  1:250824/150‑1 (MQ=255)
          gccagcATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGcccc                                                                                                                                            >  1:484065/1‑150 (MQ=255)
          gccagcATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGcccc                                                                                                                                            <  2:694184/150‑1 (MQ=255)
            cagcATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGc                                                                                                                                          >  2:3024931/1‑150 (MQ=255)
              gcATCACCGCCGCACTGTGCTTGCGGCTCCGCTCATAGCGACGCAGATAAATGTCCTCCCCGTTTTTTTCCCCCTGACGATGCAACCGTTTCAGCTCGGCAATCAGCTCTGCACCACCCAAAAACCCGAGTTTTACCCCCTGCCCCGCCa                                                                                                                                        <  2:2193168/150‑1 (MQ=255)
              gcATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCa                                                                                                                                        <  1:934168/150‑1 (MQ=255)
               cATCAGCGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTCCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAg                                                                                                                                       <  2:141311/150‑1 (MQ=255)
                  caccgccgcACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCgg                                                                                                                                    <  1:1283825/147‑1 (MQ=255)
                   aaCGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGTTCTGCAGCATCCATAAAGCCGCGATTTACCCCCTGCCCCGCCAGCggg                                                                                                                                   >  2:702973/1‑150 (MQ=255)
                     cgccgcACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTg                                                                                                                                 >  2:4025810/1‑150 (MQ=255)
                     cgccgcACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTg                                                                                                                                 >  1:2310577/1‑150 (MQ=255)
                      gccgcACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAggggaaag                                                                                                                                >  1:1909665/1‑146 (MQ=255)
                        cgcACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAAt                                                                                                                              >  2:1232733/1‑150 (MQ=255)
                            cTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTa                                                                                                                          <  1:731707/150‑1 (MQ=255)
                              gtgCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCGGACGATGCACCCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTAtg                                                                                                                        <  2:1796920/150‑1 (MQ=255)
                                gCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTAtgtg                                                                                                                      <  1:5613/150‑1 (MQ=255)
                                   tGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTgcgg                                                                                                                   >  1:2925380/1‑150 (MQ=255)
                                    gCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTgcggc                                                                                                                  <  1:4038018/150‑1 (MQ=255)
                                     cGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTgcggcg                                                                                                                 >  2:872566/1‑150 (MQ=255)
                                      ggCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGt                                                                                                                >  1:955388/1‑150 (MQ=255)
                                      ggCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGt                                                                                                                <  1:3587935/150‑1 (MQ=255)
                                        cTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCg                                                                                                              >  2:725754/1‑150 (MQ=255)
                                            gCTCATAGCGACGCAGAAAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCAAAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCcca                                                                                                          <  1:989299/150‑1 (MQ=255)
                                              tCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCccacc                                                                                                        <  1:2626491/150‑1 (MQ=255)
                                               cATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCccacca                                                                                                       >  1:4004337/1‑150 (MQ=255)
                                                aTAGCGGCGCAGATAAATGAACTTCCCGATGTCTTTCACCTGACGGTACAACCGTTTCAGCTCCGCACTCAGCTCTGCAGCATCCATAAAGCCGAGCTTTACCCCCTGCCCCGCCAGCGGGGGAATGGTATGTGCGGCGTCGCccaccac                                                                                                      >  2:1704529/1‑149 (MQ=255)
                                                aTAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAg                                                                                                      <  1:1631405/150‑1 (MQ=255)
                                                 tAGCGACGCAGATAAATGTACTGCCCGATGTTTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAgc                                                                                                     <  1:307896/150‑1 (MQ=255)
                                                 tAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAgc                                                                                                     <  2:2435686/150‑1 (MQ=255)
                                                  aGCGACGCAGATAAATGTCCTGCCCGATGTCTTTCCCCTGCCGATGCACCCGTTTCAGTCCGGCAATCAGCCCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAgcg                                                                                                    <  2:1530081/150‑1 (MQ=255)
                                                  aGCGACGCAGAAAAATGTACGGCCCGATGTCTTCCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAgcg                                                                                                    <  2:2946038/150‑1 (MQ=255)
                                                    cGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGcc                                                                                                  >  2:188674/1‑150 (MQ=255)
                                                    cGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGcc                                                                                                  >  2:927987/1‑150 (MQ=255)
                                                      aCGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAg                                                                                                >  1:4235935/1‑150 (MQ=255)
                                                      aCGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAg                                                                                                >  1:2429203/1‑150 (MQ=255)
                                                      aCGAAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCATTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAg                                                                                                <  2:205769/150‑1 (MQ=255)
                                                           gaaaaaTGTACTGCCCGATGTCTTCCCCCTGACGATGCACCCGTTTCAGTCCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGt                                                                                           <  2:1915115/147‑1 (MQ=255)
                                                           gATAAATGTACGGCCGGATGTCTTCCCCCGGACGATGCACCCGTTTCAGTTCGGCAACCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGt                                                                                           <  2:1200966/150‑1 (MQ=255)
                                                            aTAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTg                                                                                          >  1:2013703/1‑150 (MQ=255)
                                                              aaaTGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCg                                                                                        >  2:3793640/1‑150 (MQ=255)
                                                               aaTGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGa                                                                                       >  2:3874338/1‑150 (MQ=255)
                                                               aaTGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGa                                                                                       >  2:1581054/1‑150 (MQ=255)
                                                                aTGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAg                                                                                      >  2:3196271/1‑150 (MQ=255)
                                                                 tGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAgg                                                                                     >  1:2045826/1‑150 (MQ=255)
                                                                    aCTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCaa                                                                                  >  2:566502/1‑150 (MQ=255)
                                                                    aCTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGGTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGGGCTGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCaa                                                                                  >  1:1764298/1‑150 (MQ=255)
                                                                     cTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCaaa                                                                                 >  1:1585632/1‑150 (MQ=255)
                                                                      tGCCCGATGTCTTCCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAAc                                                                                <  1:3337653/150‑1 (MQ=255)
                                                                      cgCCCGATTTCTTCCCCCGACCGATGCAACCGTTCATATCCGGCAATAAGCACTGCAGCAACAAAAAAGCGGAAATTTCCCCCCGCCGCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAAc                                                                                <  2:507935/149‑1 (MQ=255)
                                                                       gCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACt                                                                               >  2:2785641/1‑150 (MQ=255)
                                                                             tGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGgcgcg                                                                         >  1:4049101/1‑150 (MQ=255)
                                                                                 ttcccccTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATa                                                                     <  1:1697860/147‑1 (MQ=255)
                                                                                        tGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGcccc                                                              >  1:3727613/1‑150 (MQ=255)
                                                                                           cGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTc                                                           <  1:681154/150‑1 (MQ=255)
                                                                                             aTGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAg                                                         >  1:2876401/1‑150 (MQ=255)
                                                                                              tGCAACCGTTTCAGTTCGGCAACCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGt                                                        <  1:2814491/150‑1 (MQ=255)
                                                                                                 aaCCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTggg                                                     >  2:2679984/1‑150 (MQ=255)
                                                                                                  aCCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGa                                                    <  1:3253356/150‑1 (MQ=255)
                                                                                                   ccGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGaa                                                   <  1:3803184/150‑1 (MQ=255)
                                                                                                    cGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAac                                                  <  1:2497003/150‑1 (MQ=255)
                                                                                                    cGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAac                                                  >  1:3173974/1‑150 (MQ=255)
                                                                                                       ttCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACAcc                                               >  1:2669960/1‑150 (MQ=255)
                                                                                                         cggTTCGGAAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTCCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTg                                             <  2:220899/148‑1 (MQ=255)
                                                                                                            ttCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGAcg                                          <  1:1358610/150‑1 (MQ=255)
                                                                                                            ttCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGAcg                                          >  2:1082745/1‑150 (MQ=255)
                                                                                                                 cAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACgcgcgc                                     >  1:1884309/1‑150 (MQ=255)
                                                                                                                  aaTCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGct                                    <  1:2172384/150‑1 (MQ=255)
                                                                                                                   aTCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGctc                                   <  2:46880/150‑1 (MQ=255)
                                                                                                                   aTCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGctc                                   <  2:1224774/150‑1 (MQ=255)
                                                                                                                    tCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGctct                                  <  2:166076/150‑1 (MQ=255)
                                                                                                                    tCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGctct                                  >  2:3185684/1‑150 (MQ=255)
                                                                                                                          ctGCAGCATCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCt                            <  2:3694591/150‑1 (MQ=255)
                                                                                                                          ctGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTAGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCt                            <  1:1186959/150‑1 (MQ=255)
                                                                                                                           tGCAGCATCCATAAAGCGGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCtt                           <  1:1006347/150‑1 (MQ=255)
                                                                                                                             cagcaTCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGc                         <  2:3997221/150‑1 (MQ=255)
                                                                                                                             cagcaTCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGc                         <  1:122239/150‑1 (MQ=255)
                                                                                                                             cagcaTCCAAAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGc                         <  2:4140745/150‑1 (MQ=255)
                                                                                                                                caTCCATAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATa                      <  1:1445328/150‑1 (MQ=255)
                                                                                                                                 atcaagaaaGCCGAGTTTGCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATaa                     <  1:1296625/144‑1 (MQ=255)
                                                                                                                                  tCCAAAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAg                    <  2:2314564/150‑1 (MQ=255)
                                                                                                                                   ccATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGc                   <  1:2469820/150‑1 (MQ=255)
                                                                                                                                   ccATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGc                   >  1:1824026/1‑150 (MQ=255)
                                                                                                                                   ccATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGc                   >  1:3476434/1‑150 (MQ=255)
                                                                                                                                   ccATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGc                   >  1:3704662/1‑150 (MQ=255)
                                                                                                                                   ccAAAAAGCCGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGc                   <  2:2845973/150‑1 (MQ=255)
                                                                                                                                    cATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGcc                  >  1:3945104/1‑150 (MQ=255)
                                                                                                                                     aTAAAGCGGAGATTTCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGccc                 <  2:3068953/150‑1 (MQ=255)
                                                                                                                                     aTAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGccc                 <  2:3237810/150‑1 (MQ=255)
                                                                                                                                       aaaGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAg               >  1:2251198/1‑150 (MQ=255)
                                                                                                                                        aaGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAgg              >  2:2251018/1‑150 (MQ=255)
                                                                                                                                                 tttCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCa     <  1:3605608/150‑1 (MQ=255)
                                                                                                                                                 tttCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCa     <  1:3534135/150‑1 (MQ=255)
                                                                                                                                                 tttCCCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCa     <  2:3457031/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAA  >  AP012306/2557923‑2558220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑