Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,758,9090TG26.2% 616.0 / 15.0 240G33G (GGT→GGG) agaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
Reads supporting (aligned to +/- strand):  ref base T (44/133);  new base G (63/0);  total (107/133)
Fisher's exact test for biased strand distribution p-value = 3.97e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 294 total aligned reads displayed.

AGCGCAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGA  >  AP012306/2758761‑2759058
                                                                                                                                                     |                                                                                                                                                      
aGCGCAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGtt                                                                                                                                                       >  1:1424486/1‑150 (MQ=255)
    cAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTAc                                                                                                                                                   <  2:2236356/150‑1 (MQ=255)
         gCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTggg                                                                                                                                              <  1:2477907/150‑1 (MQ=255)
          cAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGc                                                                                                                                             <  2:1864667/150‑1 (MQ=255)
           aGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTTCCCTGGCg                                                                                                                                            >  1:1975697/1‑150 (MQ=255)
           aGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCg                                                                                                                                            >  1:1862323/1‑150 (MQ=255)
            gCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGa                                                                                                                                           >  1:664460/1‑150 (MQ=255)
             cccGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGAt                                                                                                                                          <  1:359162/150‑1 (MQ=255)
             cccGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGAt                                                                                                                                          <  2:1784296/150‑1 (MQ=255)
              ccGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATg                                                                                                                                         <  1:26900/150‑1 (MQ=255)
              ccGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGGTg                                                                                                                                         >  2:2882465/1‑150 (MQ=255)
                    ccAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCg                                                                                                                                   <  1:1076650/150‑1 (MQ=255)
                    ccAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCg                                                                                                                                   >  1:217955/1‑150 (MQ=255)
                      aGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGG‑GGTTTTTCCTGGGGGATGTTGGCggt                                                                                                                                 >  2:397511/1‑148 (MQ=255)
                      aGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAt                                                                                                                                 <  1:2914195/150‑1 (MQ=255)
                        tAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑cc                                                                                                                              <  2:3257329/150‑1 (MQ=255)
                        tAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑cc                                                                                                                              <  2:1742020/150‑1 (MQ=255)
                         aaaaaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGAT‑CCt                                                                                                                             >  1:2106211/1‑150 (MQ=255)
                          aaaaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTg                                                                                                                            <  1:989359/150‑1 (MQ=255)
                            aaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTACC‑GGGCGATGTTGCCGAT‑CCTGaa                                                                                                                          >  2:2797282/1‑150 (MQ=255)
                            aaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGaa                                                                                                                          <  1:1918942/150‑1 (MQ=255)
                              tGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGAT‑CCTGaaaa                                                                                                                        >  1:2787811/1‑150 (MQ=255)
                                aagaagAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGGAAAAg                                                                                                                      >  1:806828/1‑150 (MQ=255)
                                aagaagAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAg                                                                                                                      <  1:3752531/150‑1 (MQ=255)
                                aagaagAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCCGGGCGGTGTTGGCGATCCCTGaaaag                                                                                                                       >  2:3778764/1‑149 (MQ=255)
                                 agaagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTTCCTGGGCGATGTTTCCGAT‑CCTGAAAAAGa                                                                                                                     >  1:1506646/1‑150 (MQ=255)
                                   aagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTTCCTGGGCGGTGTTGCCGAT‑ACTGGAAAAGaat                                                                                                                   >  2:3340535/1‑148 (MQ=255)
                                   aagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGGGAATTTGTCGAT‑CCTGgaaaataat                                                                                                                   >  2:772984/1‑146 (MQ=255)
                                       ggACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTtata                                                                                                               >  2:2779365/1‑150 (MQ=255)
                                        gACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATaa                                                                                                              >  1:3338513/1‑150 (MQ=255)
                                        gACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGGGATGTTGCCGAT‑CCTTAAAAAAATTTTTaa                                                                                                              >  2:2710015/1‑150 (MQ=255)
                                         aCTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAg                                                                                                             <  1:3154617/150‑1 (MQ=255)
                                            aCAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGgac                                                                                                          <  1:1614635/150‑1 (MQ=255)
                                            aCAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTTGGCGAAGTTGCCGAT‑CCTGAAAAAGATTTTTAAgggc                                                                                                          >  1:957238/1‑148 (MQ=255)
                                            aCAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGGGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGgac                                                                                                          >  2:119014/1‑150 (MQ=255)
                                             cAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGgacg                                                                                                         <  1:1888888/150‑1 (MQ=255)
                                              aGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGgacga                                                                                                        <  1:1577314/150‑1 (MQ=255)
                                               gCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTTACTGGGCGATGTTGCCGGT‑CCTGAAAAAAATTTATTAGgacgac                                                                                                       >  1:3046206/1‑150 (MQ=255)
                                               gCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGgacgac                                                                                                       <  1:3180102/150‑1 (MQ=255)
                                               gCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCCCAT‑CCTGAAAAAGATTTATAAGgacgac                                                                                                       >  2:2345930/1‑150 (MQ=255)
                                                cAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTACTTGGCGAAGTTGCCGAT‑CCTGAAAAAGAATTATAAGgacgacc                                                                                                      >  2:4007699/1‑149 (MQ=255)
                                                   tGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCCGAT‑CCTGAAAAAGAATTATAAGGACGACAAAc                                                                                                   >  2:1308253/1‑150 (MQ=255)
                                                   tGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTTCCGAT‑CCTGAAAAAGACTTATAAGGACGACAAAc                                                                                                   >  1:601513/1‑150 (MQ=255)
                                                      gATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTTCCTTGGCGGTGTTTCCGAT‑CCTGAAAAAGAATTTTAAGGACGACAAAACgg                                                                                                >  2:1392613/1‑150 (MQ=255)
                                                        tCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCCGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTTTAAGGACGACAAAACgggg                                                                                              >  2:507093/1‑149 (MQ=255)
                                                              aTCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAgc                                                                                        <  1:678933/150‑1 (MQ=255)
                                                              aTCAGTAAAAAAGATATCAACCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTTACTGGGCGATGTTGCCGGT‑CCTGAAAAAGATTTATAAAGACGACAAA‑CGGGCTTAAgcg                                                                                       >  2:3665182/1‑150 (MQ=255)
                                                                  gTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCgcgg                                                                                    <  2:2575129/150‑1 (MQ=255)
                                                                    aaaaaaGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGGTGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCgcggcg                                                                                  >  1:1537811/1‑150 (MQ=255)
                                                                      aaaaGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGGGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAACGCGGCGAt                                                                                >  2:559640/1‑150 (MQ=255)
                                                                      aaaaGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGAt                                                                                <  1:417724/150‑1 (MQ=255)
                                                                        aaGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGa                                                                              <  1:2624936/150‑1 (MQ=255)
                                                                          gATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGaaa                                                                            <  1:3546776/150‑1 (MQ=255)
                                                                           atatCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTTCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAg                                                                           >  1:3463545/1‑150 (MQ=255)
                                                                            tatCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCCGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAAGACGACAAACCGGGCTTAAGCGCGGCGGTGaaaaa                                                                          >  2:1411595/1‑148 (MQ=255)
                                                                             atCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGAt                                                                         >  1:2388815/1‑150 (MQ=255)
                                                                              tCACCCGTATGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATa                                                                        <  1:1708732/150‑1 (MQ=255)
                                                                                aCCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAAc                                                                      >  1:3792857/1‑150 (MQ=255)
                                                                                 cccGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAAcc                                                                     >  1:4057139/1‑150 (MQ=255)
                                                                                 cccGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAAcc                                                                     <  1:3944295/150‑1 (MQ=255)
                                                                                     tCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGa                                                                 <  1:2404844/150‑1 (MQ=255)
                                                                                     tCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGa                                                                 <  2:1896153/150‑1 (MQ=255)
                                                                                     tCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGGTGTTTCCGAT‑CCTGAAAAAAATTTATAAAGACGACAAAACGGGCTTTAACGCGGCGATGAAAAATAAACTCGa                                                                 >  2:4045399/1‑150 (MQ=255)
                                                                                      cTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGaa                                                                >  1:1299563/1‑150 (MQ=255)
                                                                                       tGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCCGGGCGGTGTTGCCGAT‑CCTGGAAAAAAATTTTAAGGACGACAAACCCGGGTTAAACGCGGCGGTGAAAGATAAACTCGAAt                                                               >  2:1459551/1‑150 (MQ=255)
                                                                                          gCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATtta                                                            <  2:2527195/150‑1 (MQ=255)
                                                                                           cTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGGTGGTGGCGAT‑CCTTAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGACGAAAGATAACCTCGAATttat                                                           >  2:1699499/1‑150 (MQ=255)
                                                                                           cTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTTCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAAACTCGAATttat                                                           >  1:195848/1‑150 (MQ=255)
                                                                                            tttCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑CGGTTTTCCTGGGCGGTGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAAACCGGCCTAAGCGCGGCGATGGAAGATAAACTCGAATttatt                                                          >  2:2396816/1‑150 (MQ=255)
                                                                                                gttcgtCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATa                                                      >  1:220350/1‑150 (MQ=255)
                                                                                                 ttcgtCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATac                                                     <  1:2024574/150‑1 (MQ=255)
                                                                                                 ttcgtCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAAACTCGAATTTATTAATac                                                     >  2:344900/1‑150 (MQ=255)
                                                                                                      cgCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATacccacc                                                <  1:1961943/150‑1 (MQ=255)
                                                                                                       gctgctGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAAACTCGAATTTATTAATaaccaccc                                               >  2:3906799/1‑150 (MQ=255)
                                                                                                        ctgctgCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAAACGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTTATacccccccc                                              >  2:3706070/1‑149 (MQ=255)
                                                                                                           ctgcAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAAt                                           <  1:1001026/150‑1 (MQ=255)
                                                                                                           ctgcAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAAt                                           <  1:4216886/150‑1 (MQ=255)
                                                                                                               aaGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATaa                                                                       >  2:1585144/1‑118 (MQ=255)
                                                                                                                 gCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTc                                     <  2:3115165/150‑1 (MQ=255)
                                                                                                                 gCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTc                                     <  1:2543108/150‑1 (MQ=255)
                                                                                                                 gCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGGGATGTTGCCGCG‑CCTGAAAAAGATTTATAAGGGCGACAAACCGGGCTTAAGCGGGGGGGTGAAAGGTAACCCCGAATTTTTTAATACCCACCCGGACCTGGTc                                     >  2:1453580/1‑150 (MQ=255)
                                                                                                                     gCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGAt                                 <  2:2495682/150‑1 (MQ=255)
                                                                                                                      cTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGAtt                                >  1:3825697/1‑150 (MQ=255)
                                                                                                                      cTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGAtt                                <  1:1209619/150‑1 (MQ=255)
                                                                                                                       tttAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGGGATGAAAGATAAACTCGAATTTATTAATACCCACCCCAATCTGGGCGGGTTc                               >  2:936113/1‑150 (MQ=255)
                                                                                                                             tACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATg                         <  1:3432294/150‑1 (MQ=255)
                                                                                                                               cGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATggg                       <  2:3804770/150‑1 (MQ=255)
                                                                                                                                gAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGGGATGTTGCCGGT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGAGGAAAAATAACCTCCAATTTATTAATAACCACCCGCATCTGGTCGGATTCCTGATgggg                      >  2:3309228/1‑150 (MQ=255)
                                                                                                                                gAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGGCGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATgggg                      >  2:3542256/1‑150 (MQ=255)
                                                                                                                                 aaaGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCCGAT‑CCTGAAAAAAATTTTTAAGGACGACAAAACGGGCTTAAGCGCGGCGGTGAAAGATAACCTCGAATTTATTAATACCCACCCCAATCTGGTTGGGTTTCTGGTggggg                     >  2:3018085/1‑149 (MQ=255)
                                                                                                                                  aaGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGtt                    <  2:2006523/150‑1 (MQ=255)
                                                                                                                                      aTGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGttatta                <  1:1234835/150‑1 (MQ=255)
                                                                                                                                         cAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGGTATTAAtt             >  2:2964503/1‑150 (MQ=255)
                                                                                                                                              gggc‑ggTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGAt        <  2:3746358/150‑1 (MQ=255)
                                                                                                                                              gggc‑ggTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGAt        >  1:4101793/1‑150 (MQ=255)
                                                                                                                                              gggc‑ggTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGAt        >  1:1275332/1‑150 (MQ=255)
                                                                                                                                               ggc‑ggTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATg       >  2:4147687/1‑150 (MQ=255)
                                                                                                                                               ggc‑ggTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATg       <  1:1034912/150‑1 (MQ=255)
                                                                                                                                                 c‑ggTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGga     <  1:2373658/150‑1 (MQ=255)
                                                                                                                                                   ggTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaa    >  1:4287319/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AGCGCAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAT‑CCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGA  >  AP012306/2758761‑2759058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑