Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,268,8510GA22.6% 783.1 / 74.3 306S119F (TCC→TTC) nlpAcytoplasmic membrane lipoprotein‑28
Reads supporting (aligned to +/- strand):  ref base G (200/37);  new base A (0/69);  total (200/106)
Fisher's exact test for biased strand distribution p-value = 1.04e-41
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 330 total aligned reads displayed.

CGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAA  >  AP012306/3268705‑3269000
                                                                                                                                                   |                                                                                                                                                      
cgGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AAt                                                                                                                                                    <  1:1030761/150‑1 (MQ=255)
cgGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AAt                                                                                                                                                    >  2:1985832/1‑150 (MQ=255)
cgGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AAt                                                                                                                                                    >  2:263277/1‑150 (MQ=255)
 ggtaggtaATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATa                                                                                                                                                   <  1:1479721/150‑1 (MQ=255)
  gtaggtaATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATaa                                                                                                                                                  <  2:1836737/150‑1 (MQ=255)
   taggtaATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAAc                                                                                                                                                 <  1:1724636/150‑1 (MQ=255)
    aggtaATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAAcc                                                                                                                                                >  1:2746682/1‑150 (MQ=255)
      gtaATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCg                                                                                                                                              <  2:615896/150‑1 (MQ=255)
      gtaATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCg                                                                                                                                              >  1:3945347/1‑150 (MQ=255)
        aataaCCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGcc                                                                                                                                            >  1:3383077/1‑150 (MQ=255)
                ttttCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGaa                                                                                                                                    >  2:1116038/1‑150 (MQ=255)
                  ttCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGa                                                                                                                                  >  2:3571734/1‑150 (MQ=255)
                    cccTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACa                                                                                                                                >  2:1575775/1‑150 (MQ=255)
                     ccTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACaa                                                                                                                               >  2:695849/1‑150 (MQ=255)
                       tctTTCAGAGTAATTAGCTTCTTTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTGGGGGGGGTTGTGGGGGATCGCCACTGTCGCGCCTTTTTTTATTTGCGCCACGT‑TTTTGTTTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAAc                                                                                                                             <  2:3879617/150‑1 (MQ=255)
                        ctTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACg                                                                                                                            >  2:1944760/1‑150 (MQ=255)
                        ctTTAAGAGTATTTACTTTCTTTTTTTACACCAGAAAAAGGGCGCGACCAGGGTTGGTGGGGTTGTTGGGGACCCCCCCTGCCGCGCTTTTTTTTATTTGCCCCAGGT‑TTTTTTTTTTTTTGA‑AAAAACCCGCCATAGGGAAGACAAACg                                                                                                                            <  2:3010381/150‑1 (MQ=255)
                         tttCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGt                                                                                                                           >  1:2213386/1‑150 (MQ=255)
                           tCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGCAt                                                                                                                         >  1:260159/1‑150 (MQ=255)
                             agagTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTAttt                                                                                                                       >  1:4065094/1‑150 (MQ=255)
                              gagTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTc                                                                                                                      >  2:3402176/1‑150 (MQ=255)
                               agTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTcc                                                                                                                     >  2:3322268/1‑150 (MQ=255)
                               agTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTcc                                                                                                                     >  1:2815689/1‑150 (MQ=255)
                                 tAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGAAAGACAAACGTATTTCCCa                                                                                                                   >  2:2840284/1‑150 (MQ=255)
                                    ttAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCg                                                                                                                >  2:2057520/1‑150 (MQ=255)
                                      aGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCt                                                                                                              >  1:1097570/1‑150 (MQ=255)
                                        cttcttTTTTTGCAACAGAAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGACCCCCCCTGTCGCCCTTTTTTTTTTTTGCGCCAC‑G‑TTTTTATTTTTTTGGAAATACCCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTAc                                                                                                            <  2:1706956/150‑1 (MQ=255)
                                         ttCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTAcc                                                                                                           >  1:807617/1‑150 (MQ=255)
                                          tctcTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCa                                                                                                          >  2:212214/1‑150 (MQ=255)
                                           ctctTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAg                                                                                                         >  1:3721814/1‑150 (MQ=255)
                                           ctctTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAg                                                                                                         >  1:365027/1‑150 (MQ=255)
                                            tttttttGAAACAGAAAAGGGGCGCGACCAAGGTTGGTGGGGTTGTGGGGGCTCGCCCCTGTCCCCCCTTTTTTTATTTGCGCCACGT‑TTTTGTTTTTTTGGA‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGt                                                                                                        <  2:1207148/148‑1 (MQ=255)
                                             ctTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGtt                                                                                                       >  1:1763657/1‑150 (MQ=255)
                                              tttttGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGttt                                                                                                      >  1:2968191/1‑150 (MQ=255)
                                               ttttGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTta                                                                                                     >  1:603762/1‑150 (MQ=255)
                                                tttGCAACAGTAAAAGGGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCTTTTTTTTATTTGCGCCACGT‑TTTTGTTTTTTTTGA‑AATACCCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTtat                                                                                                    <  1:2363344/150‑1 (MQ=255)
                                                  tGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGGCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATaa                                                                                                  >  1:621104/1‑150 (MQ=255)
                                                  tGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATaa                                                                                                  >  2:303891/1‑150 (MQ=255)
                                                   gaaaCAGTAAAGGGCCGCGACCAGGGTGGGTGGGGTTGTGGGGGATCGCCACTGCCGCGCCTTTTTTTATTTGCGCCACGG‑TTTTGTTTTTTTTGA‑AAAAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAAc                                                                                                 <  2:1058330/148‑1 (MQ=255)
                                                   gCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAAc                                                                                                 >  1:300599/1‑150 (MQ=255)
                                                    cAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAAcc                                                                                                >  2:3626496/1‑150 (MQ=255)
                                                     aaCAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCa                                                                                               >  2:8713/1‑150 (MQ=255)
                                                     aaCAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCa                                                                                               >  1:3882740/1‑150 (MQ=255)
                                                       cAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATg                                                                                             >  2:3141193/1‑150 (MQ=255)
                                                        aGTAAAGGGGCCCAACAAAGGTTGGCCGGGTGTTTGGGGATCGCCACTGCCGCCCTTTTTTTTATTTGCCCCAGGT‑TTTTTTTTTTTTTGA‑AATACCCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATgc                                                                                            <  2:654170/150‑1 (MQ=255)
                                                         gTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATgcg                                                                                           >  2:509566/1‑150 (MQ=255)
                                                             aaGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTg                                                                                       >  1:3533199/1‑150 (MQ=255)
                                                               gTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGAt                                                                                     >  1:1180393/1‑150 (MQ=255)
                                                                 gcgcGGCAAGGGTGGGTGGGGTTGTTGGGGATCGCCGTGGTCCCGCTTTTTTTTATTGGCCCCGTGG‑TTTTGATTTTTTTGA‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTa                                                                                   <  2:3760535/150‑1 (MQ=255)
                                                                         aggtttGGTGGGGTGGTGGGGGTTCCCCCCTGTCGCGCTTTTTTTTATTTGCGCCCGGT‑TTTTTTTTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTc                                                                           <  2:1661232/146‑1 (MQ=255)
                                                                          aGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCa                                                                          >  1:1645864/1‑150 (MQ=255)
                                                                           ggtgggtgGGGTCGTTGGGGACCCCCACTGCCGCGCCTTCTTTTATTTGCGCCGCGG‑TTTTGATTTTTTTGA‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCaa                                                                         <  2:3971104/150‑1 (MQ=255)
                                                                            gTTGGTGGGGTGTTGGGGGTTCGCCCCTGTCGCTCCTTTTTTTTTTTGCGCCACGG‑TTTTTTTTTTTTTGA‑AATACCCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAg                                                                        <  2:2679903/150‑1 (MQ=255)
                                                                            gTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAg                                                                        >  2:2558226/1‑150 (MQ=255)
                                                                              tGGTCGGGTCGTGGGGGATCGCCGCTGTCGCGCTTTTTTTTATTTGCCCCACGG‑TTTTTATTTTTTTGA‑AATACCCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGaa                                                                      <  2:788978/150‑1 (MQ=255)
                                                                               ggtcgggtcgTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGaaa                                                                     >  1:3909994/1‑150 (MQ=255)
                                                                                  cgggtcgTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAggg                                                                  >  1:1621682/1‑150 (MQ=255)
                                                                                  cgggtcgTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAggg                                                                  >  2:1332835/1‑150 (MQ=255)
                                                                                   gggttgTGGGGGTTCCCCACTGCCGCGCTTTTTTTTATTTGCCCCTGGT‑TTTTGTTTTTTTTGA‑AATACCCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGt                                                                 <  2:36478/150‑1 (MQ=255)
                                                                                   gggtcgTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGt                                                                 >  1:2494089/1‑150 (MQ=255)
                                                                                    ggtggtGGGGGATCGCCACTGTCGCGCCTTTTTTTATTTGCGCCACGT‑TTTTGTTTTTTTTGA‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTc                                                                <  2:1540302/150‑1 (MQ=255)
                                                                                    ggtggtGGGGGATCCCCACTGTCGCGCTTTTTTTTATTGGCCCCAGGT‑TTTTGTTTTTTTTGA‑AATACCCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTc                                                                <  2:1394910/150‑1 (MQ=255)
                                                                                        tttGGGGCCCCCCCCTCTCGCCCTTTTTTTTTTTTCCCCCCCGT‑TTTTTTTTTTTTTGA‑AATACCCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATg                                                            <  2:2385276/149‑1 (MQ=255)
                                                                                        gTTGGGGACCGCCACTGTCGCGCTTTCTTTTATTTGCGCCATGT‑TTTTGATTTTTTGGA‑AAAAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATg                                                            <  1:378114/150‑1 (MQ=255)
                                                                                          gggggACCCCCACTGCCGCGCTTTTTTTTATTTGCGCCACGT‑TTTTGTTTTTTTTGA‑AATACCCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCt                                                          <  2:4258538/149‑1 (MQ=255)
                                                                                                tCGCCACTGTCGCGCTTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGa                                                    <  1:2658774/150‑1 (MQ=255)
                                                                                                  gCCACTGTCGCGCTTTTTTTTATTTGCGCCACGT‑TTTTGATTTTTTTGA‑AATACCCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACa                                                  <  2:2492803/150‑1 (MQ=255)
                                                                                                    cACTGCCGCCCTTTCTTTTATTTGCGCCACGT‑TTTTGATTTTTTTGA‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACAtt                                                <  2:2593513/150‑1 (MQ=255)
                                                                                                      cTGCCGCGCTTTTTTTTATTTGCGCCCCGT‑TTTTGTTTTTTTTGG‑AAAAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCg                                              <  1:3059788/150‑1 (MQ=255)
                                                                                                       tGTCGCGCTTTCTTTTATTGGCGCCTCGT‑TTTTGTTTTTTTTGA‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGc                                             <  1:1333721/150‑1 (MQ=255)
                                                                                                       tGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGc                                             >  1:1430986/1‑150 (MQ=255)
                                                                                                           gcgcCTGTTTTTAGTTGCGCCGGGT‑TTTTGTTTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCa                                         <  1:185146/150‑1 (MQ=255)
                                                                                                             gcCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGGAGACATTCGCATCAAg                                       >  2:3499129/1‑150 (MQ=255)
                                                                                                             gcCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAg                                       >  1:2860440/1‑150 (MQ=255)
                                                                                                              ccTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGt                                      <  1:504177/150‑1 (MQ=255)
                                                                                                              ccTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGt                                      >  1:903491/1‑150 (MQ=255)
                                                                                                                tttttttttttGCCCCCCGG‑TTTTGTTTTTTTTGA‑AATACCCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTc                                    <  2:4104839/147‑1 (MQ=255)
                                                                                                                ttcttTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTc                                    >  1:3286966/1‑150 (MQ=255)
                                                                                                                        tttGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGAt                            >  1:997612/1‑150 (MQ=255)
                                                                                                                         tttggcCCCGG‑TTTTTTTTTTTTTGGAAACACCCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGAt                            <  2:4238215/146‑1 (MQ=255)
                                                                                                                         ttGCGCCACGT‑TTTTGTTTTTTTTGA‑AATACCCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGAtt                           <  1:2057520/150‑1 (MQ=255)
                                                                                                                         gtGCCCCGCGT‑TTTTAATTTTTTTGA‑AAAAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGAtt                           <  2:1253460/149‑1 (MQ=255)
                                                                                                                          tGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGAttt                          >  2:3221732/1‑150 (MQ=255)
                                                                                                                            gcgcACGGTTTTTAATTTTTTAGA‑AATACCCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTg                         <  1:4189225/147‑1 (MQ=255)
                                                                                                                               cACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGc                     >  2:3792094/1‑150 (MQ=255)
                                                                                                                               cACGG‑ATTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGGATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGc                     >  2:3706542/1‑150 (MQ=255)
                                                                                                                                  gg‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGCATc                  >  1:4166441/1‑150 (MQ=255)
                                                                                                                                  gg‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGCATc                  >  2:4172939/1‑150 (MQ=255)
                                                                                                                                  gg‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGAAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGCATc                  >  2:2431706/1‑150 (MQ=255)
                                                                                                                                   g‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGCATCg                 >  1:4292796/1‑150 (MQ=255)
                                                                                                                                    tttttTATTTTTGTGA‑AAAACCCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGCATCg                 <  2:2311335/149‑1 (MQ=255)
                                                                                                                                     ttttTATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGCATCGt                >  1:3235889/1‑150 (MQ=255)
                                                                                                                                       ttGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTc              <  1:2116362/150‑1 (MQ=255)
                                                                                                                                         gATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCgg            >  1:1285143/1‑150 (MQ=255)
                                                                                                                                          aTTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGt           >  1:2886661/1‑150 (MQ=255)
                                                                                                                                           tttttttGA‑AAAAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTa          <  2:3937991/150‑1 (MQ=255)
                                                                                                                                             tttttGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGc        >  1:694169/1‑150 (MQ=255)
                                                                                                                                             tttttGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGc        >  2:1298227/1‑150 (MQ=255)
                                                                                                                                                ttGG‑AAAAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAAc     <  1:1958944/150‑1 (MQ=255)
                                                                                                                                                  gg‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGa   >  2:2266579/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
CGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGG‑TTTTGATTTTTTTGG‑AATAACCCGCCATAGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCTTGTTCAAGAAAGGGTCGATGCTGGAAGACATTCGCATCAAGTTCGCCATGATTTGTCGCATCGTTCGGTAGCAACGAA  >  AP012306/3268705‑3269000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑