Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,324,4330TA22.6% 607.4 / 86.2 239intergenic (+2/+52)yieF/yieGchromate reductase, Class I, flavoprotein/predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (147/38);  new base A (0/54);  total (147/92)
Fisher's exact test for biased strand distribution p-value = 5.78e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 269 total aligned reads displayed.

GGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTC  >  AP012306/3324284‑3324581
                                                                                                                                                     |                                                                                                                                                     
ggTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCtaaat                                                                                                                                                       <  2:1874172/150‑1 (MQ=255)
 gTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCtaaata                                                                                                                                                      <  1:1693169/150‑1 (MQ=255)
 gTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCtaaata                                                                                                                                                      <  1:2677196/150‑1 (MQ=255)
   gatgaACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCtaaataaa                                                                                                                                                    <  2:3347772/150‑1 (MQ=255)
    atgaACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATaaaa                                                                                                                                                   >  2:1498579/1‑150 (MQ=255)
     tgaACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATaaaaa                                                                                                                                                  >  2:583443/1‑150 (MQ=255)
      gaACAAGCCGGGATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATaaaaaa                                                                                                                                                 >  2:1350082/1‑150 (MQ=255)
      gaACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATaaaaaa                                                                                                                                                 >  2:1672307/1‑150 (MQ=255)
      gaACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATaaaaaa                                                                                                                                                 >  1:2472665/1‑150 (MQ=255)
        aCAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑cc                                                                                                                                              <  1:4096638/150‑1 (MQ=255)
         cAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑ccc                                                                                                                                             >  1:3340509/1‑150 (MQ=255)
         cAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑ccc                                                                                                                                             <  1:3209749/150‑1 (MQ=255)
           aGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGc                                                                                                                                           >  2:3858023/1‑150 (MQ=255)
           aGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGc                                                                                                                                           <  2:1930085/150‑1 (MQ=255)
                gAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCa                                                                                                                                      >  1:3372848/1‑150 (MQ=255)
                  aTTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAAt                                                                                                                                    >  2:4107177/1‑150 (MQ=255)
                   tttATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATc                                                                                                                                   >  2:1961049/1‑150 (MQ=255)
                    ttATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATca                                                                                                                                  >  1:365052/1‑150 (MQ=255)
                          gcggcgTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATcatgcatg                                                                                                                            >  1:2754569/1‑150 (MQ=255)
                              cgTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCgg                                                                                                                        >  1:596680/1‑150 (MQ=255)
                                 gATTCAGAACAAAGTGGATCCGCAAACCGGAGAAGTGATTGATAGGGTTACGCTGGACCACCTGACCGGGCAATTGCCCGCATTTGGTGATTTTTTCCAGGGAGTAAAGATCAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGtt                                                                                                                     <  2:4035943/150‑1 (MQ=255)
                                   ttCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGtttt                                                                                                                   >  2:3213192/1‑150 (MQ=255)
                                    tCATAAAAAAGTTGACCCGAAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCCCCTGACGGGACAATTGCCCGCATTTGGTGAGTTTATTCAGGGAGTTAAGATCTAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGttttt                                                                                                                  <  2:346643/150‑1 (MQ=255)
                                    tCAAAACAAGGTTGATCCCAAAACGGGAGAGGTAATTGATAGGGTTACGGTGGACCCCCGGACCGGGAAATTGACCGCTTTTGGTGAGTTTATTCGGAGATTTAAGTTCAAAAAAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGttttt                                                                                                                  <  2:1806905/150‑1 (MQ=255)
                                    tCAAAAAAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGAAATTGACCGCTTTTGGTGATTTTATTCAGGGAGTTAAGATCAAAAAAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGttttt                                                                                                                  <  2:2253688/150‑1 (MQ=255)
                                     cAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATGGGGGTACGCTGGACCACCGGACCGGGCAATTGACCGCTTTTGGTGAGTTTATTAGGGGAGTTAAAATCTAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTa                                                                                                                 <  2:2235566/150‑1 (MQ=255)
                                          cAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGCTTTTAAcgcg                                                                                                            >  1:3948739/1‑150 (MQ=255)
                                            aaGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCt                                                                                                          >  2:829006/1‑150 (MQ=255)
                                             aGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTa                                                                                                         >  1:1385952/1‑150 (MQ=255)
                                               ttGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATc                                                                                                       >  1:3910015/1‑150 (MQ=255)
                                                tGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCTATCg                                                                                                      >  2:3653282/1‑150 (MQ=255)
                                                tGATCCGAAAACGGGAGAAGTGATTGATCGGGGTACGCGGGCCCCCCTGACGGGGAAATTGACCGCTTTTGGTGAGTTTATTCAGGGAGTTAAGATCAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCg                                                                                                      <  2:2192963/150‑1 (MQ=255)
                                                    ccGAAAACGGGAGAAGTGTTTGATGAGGGAACGCGGGACCCCTTGCCGGGTGATTTGGCCGCATGTGGTGAGTTTAATCAGGGATTTAAGATCAAAAAAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGAttt                                                                                                  <  2:977933/150‑1 (MQ=255)
                                                     cgaaaaCGGGAGAGGTGATTGTCCAGGGTACGCGGGCCCACCGGACCGGGAAATTGTCCGCATTTGGTGGGTTTATTCAGCGATTTAAATTCAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGAtttt                                                                                                 <  2:2946414/147‑1 (MQ=255)
                                                     cGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCCATCGAattt                                                                                                 >  2:2890271/1‑150 (MQ=255)
                                                      gaaaaCCGGAGAAGTGATTGATCAGGTTACGCTGGACCACCTGACCGGGAAATTGCCCGAATTTGGTGAGTTTATTCAGCGATTAAAGA‑CTAAAAAAAAAACCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTa                                                                                                <  2:433817/148‑1 (MQ=255)
                                                      gCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTa                                                                                                >  1:187932/1‑150 (MQ=255)
                                                        aagccGGAGAGGTAATTGATGAGGGTAGGCTGGACCCCCAGCCGGGGAATTTGCCAGCTTTTGGGGATTTTATTAAGAGAGTTAAGATAAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGt                                                                                              <  2:2657183/147‑1 (MQ=255)
                                                        aaaCCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTTAAGCGCTATCGGTTTTAGt                                                                                              >  2:873995/1‑150 (MQ=255)
                                                        aaaCCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGt                                                                                              >  1:2242470/1‑150 (MQ=255)
                                                         aaCCGGAGAAGTGATTGATCGGGGTACGCGGGACCCCCTGACCGGGCAATTGACCGTTTTTGGTGATTTTATTCAGCGAGTTAAGATATAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTg                                                                                             <  2:1434999/150‑1 (MQ=255)
                                                            cGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGc                                                                                          >  1:2584980/1‑150 (MQ=255)
                                                            cGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGCTTTTAACCCGCTATCGATTTTAGTGAGc                                                                                          >  1:4280600/1‑150 (MQ=255)
                                                             ggAGAAGTGATTGATCAGGGTCCGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCAAAATAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCg                                                                                         <  1:3392544/150‑1 (MQ=255)
                                                             ggAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCg                                                                                         >  2:3724885/1‑150 (MQ=255)
                                                              gagaAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGt                                                                                        >  1:3615902/1‑150 (MQ=255)
                                                                gaAGTGATTGATGAGGGTCCGCTGGACCACCTGCCCGGGCAATTGCCCGTTTTTGGTGATTTTATTCAGCGAGTTAAGATCAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCg                                                                                      <  2:726850/150‑1 (MQ=255)
                                                                    tgattgatCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATaa                                                                                  >  2:3391530/1‑150 (MQ=255)
                                                                     gattgatCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATaaa                                                                                 >  1:1123776/1‑150 (MQ=255)
                                                                     gattgatCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATaaa                                                                                 >  1:2148097/1‑150 (MQ=255)
                                                                        tgatCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGAc                                                                              >  2:2404613/1‑150 (MQ=255)
                                                                         gatCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACa                                                                             >  1:3311772/1‑150 (MQ=255)
                                                                         gatCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGGTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACa                                                                             >  2:2749033/1‑150 (MQ=255)
                                                                           tCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAAAAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAAt                                                                           <  2:359989/150‑1 (MQ=255)
                                                                             aGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCt                                                                         >  1:3736443/1‑150 (MQ=255)
                                                                              gggTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCtt                                                                        >  1:3858770/1‑150 (MQ=255)
                                                                              gggTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGGGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGGTTTTAAAGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCtt                                                                        >  2:569119/1‑150 (MQ=255)
                                                                                  ccgcgGGCCCACCTGACCGGGCAATTGCCCGCTTTTGGGGAGTTTATTCAGCGAGTTAAGATCAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAgg                                                                    <  2:3046708/149‑1 (MQ=255)
                                                                                    gCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGAt                                                                  >  1:2541911/1‑150 (MQ=255)
                                                                                     cTGGACCACCTGCCCGGGAAGTTGACCGCATTGGGTGATTTTATTCGGGGAGTTAAGATCAAAAAAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATa                                                                 <  2:1219077/150‑1 (MQ=255)
                                                                                      tgaacccccTGACGGGGAAATTGCCCGCATTTGGTGAGTTTTTTCAGCGAGTTAAAATATAAAAAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATaa                                                                <  2:1624951/147‑1 (MQ=255)
                                                                                       ggACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATaaa                                                               >  1:3254794/1‑150 (MQ=255)
                                                                                       ggACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATaaa                                                               >  1:3504618/1‑150 (MQ=255)
                                                                                          ccaccTGACCGGAAAATTGACCGCTTTTGGGGATTTTATTCAGCGAGTTAAGATCAAAAAAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAcag                                                            <  2:4186036/150‑1 (MQ=255)
                                                                                            accTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGTATAAacaaca                                                          >  1:3242865/1‑150 (MQ=255)
                                                                                               tgcccGGGCAATTGACCGCTTTTGGTGATTTTATTCAGCGATTTAAGATAAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAgcg                                                       <  2:2033605/147‑1 (MQ=255)
                                                                                               tGACGGGGCAATGGACCGCATTTGGTGGTTTTATTCAGCGATTTAAGATCTAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAgcg                                                       <  2:3084207/150‑1 (MQ=255)
                                                                                                  ccGGGAAATTGCCCGCTTTTGGGGATTTTATTCAGCGAGTTAAGATCTAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCaa                                                    <  2:1600667/150‑1 (MQ=255)
                                                                                                    gggCAATTGACCGCTTTTGGTGATTTTATTCAGGGAGTTAAGATTTAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACg                                                  <  2:2579588/150‑1 (MQ=255)
                                                                                                     ggCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACga                                                 >  2:1051729/1‑150 (MQ=255)
                                                                                                      gCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACgat                                                >  1:554607/1‑150 (MQ=255)
                                                                                                       cAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACgatg                                               >  1:864387/1‑150 (MQ=255)
                                                                                                            gACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTAc                                          >  1:906438/1‑150 (MQ=255)
                                                                                                              ccGCATTTGGTGATTTTATTCAGCGAGTTAAGATCAAAAAAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGc                                        <  2:3280770/150‑1 (MQ=255)
                                                                                                              ccGAATTGGGTGGTTTTATTAAGGGAGTTAAGATAAAAAAAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGc                                        <  2:955956/150‑1 (MQ=255)
                                                                                                               cGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCa                                       >  1:3065685/1‑150 (MQ=255)
                                                                                                                 cttttGGTGATTTTATCCAGCGAGTTAAAATCAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACg                                     <  2:3226647/148‑1 (MQ=255)
                                                                                                                 cATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACg                                     >  1:3811799/1‑150 (MQ=255)
                                                                                                                 cATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAAAGATGATTACGCACg                                     >  1:2062086/1‑150 (MQ=255)
                                                                                                                 cATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGGCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACg                                     >  2:4219458/1‑150 (MQ=255)
                                                                                                                  aTTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACgg                                    >  2:490914/1‑150 (MQ=255)
                                                                                                                  aTTTGGGGATTTTATTCAGCGAGTTAAGATCAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACgg                                    <  2:382898/150‑1 (MQ=255)
                                                                                                                   tttGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGAGAGCGTCGATAAAGACAAACTTCAGGAAAAACAGCAGGGCAACGGTGATTAAGCCCggg                                   >  2:1560211/1‑150 (MQ=255)
                                                                                                                      ggTGATTTTATTCAGCGATTTAAGATAAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCtt                                <  2:1914507/150‑1 (MQ=255)
                                                                                                                       gTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTa                               <  1:1470698/150‑1 (MQ=255)
                                                                                                                        tGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAg                              >  1:2329453/1‑150 (MQ=255)
                                                                                                                          aGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGAt                            >  1:1357349/1‑150 (MQ=255)
                                                                                                                          aGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGAt                            >  1:1549944/1‑150 (MQ=255)
                                                                                                                           gTTTATTCAGCGATTTAAAATCAAAATAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATc                           <  2:2509016/150‑1 (MQ=255)
                                                                                                                               aTTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGc                       >  1:801375/1‑150 (MQ=255)
                                                                                                                                  cAGCGAGTTAAGATCTAAATAAAAAA‑CACGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGa                    <  1:2948793/150‑1 (MQ=255)
                                                                                                                                    gggatttAAGATCTAAATAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACg                  <  1:2871317/145‑1 (MQ=255)
                                                                                                                                     cGAGTAAAGATGAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGt                 <  2:2791201/150‑1 (MQ=255)
                                                                                                                                       ggTTAAGATCAAAAAAAAAAC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTcc               <  2:887650/149‑1 (MQ=255)
                                                                                                                                       aGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCCTAGATCACGCAGACGTcc               >  1:103752/1‑150 (MQ=255)
                                                                                                                                         ttAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCgg             >  1:1284113/1‑150 (MQ=255)
                                                                                                                                           aaGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAATGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAAATCACGCAGACGTCCGGTa           >  2:1107835/1‑150 (MQ=255)
                                                                                                                                            aGATATAAATAAAACC‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTAc          <  2:3578241/150‑1 (MQ=255)
                                                                                                                                               tCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGa       >  2:2291087/1‑150 (MQ=255)
                                                                                                                                                cTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGAt      >  2:687250/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAA‑CCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTC  >  AP012306/3324284‑3324581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑