Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 3,478,222 C→A R682S (CGT→AGT)  polA → fused DNA polymerase I 5'‑>3' exonuclease/3'‑>5' polymerase/3'‑>5' exonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,478,2220CA100.0% 1376.8 / NA 340R682S (CGT→AGT) polAfused DNA polymerase I 5'‑>3' exonuclease/3'‑>5' polymerase/3'‑>5' exonuclease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (167/173);  total (167/173)

Only 100 of 343 total aligned reads displayed.

CTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGCGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTGACCGCATTCGCGGAAGGA  >  AP012306/3478075‑3478368
                                                                                                                                                   |                                                                                                                                                  
cTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGt                                                                                                                                                  >  1:4248348/1‑150 (MQ=255)
cTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGt                                                                                                                                                  >  2:411932/1‑150 (MQ=255)
   gCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAAc                                                                                                                                               <  1:4086480/150‑1 (MQ=255)
    cGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACg                                                                                                                                              >  1:3663565/1‑150 (MQ=255)
     gAAGCTGAAATCGACCTACACCGACAAGCTGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACga                                                                                                                                             >  2:213145/1‑150 (MQ=255)
       aGCTGAAATCGACCTACACCGACAAGCTGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACgaag                                                                                                                                           <  1:688357/150‑1 (MQ=255)
        gCTGAAATCGACCTACACCGACAAGCTGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACgaaga                                                                                                                                          >  1:316819/1‑150 (MQ=255)
         cTGAAATCGACCTACACCGACAAGCTGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACgaagta                                                                                                                                         <  1:1072226/150‑3 (MQ=255)
          tGAAATCGACCTACACCGACAAGCTGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGaagaag                                                                                                                                        <  2:315607/150‑1 (MQ=255)
                   ccTACACCGACAAGCTGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTa                                                                                                                               <  2:2674444/150‑1 (MQ=255)
                     tACACCGACAAGCTGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATc                                                                                                                             >  1:3204622/1‑150 (MQ=255)
                      acacCGACACGCTGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATcc                                                                                                                            >  2:1582946/1‑150 (MQ=255)
                      acacCGACAAGCTGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATcc                                                                                                                            <  2:1685000/150‑1 (MQ=255)
                         ccGACAAGCTGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGcc                                                                                                                         <  2:3274489/150‑1 (MQ=255)
                           gACAAGCTGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAg                                                                                                                       >  1:1649338/1‑150 (MQ=255)
                              aaGCTGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCg                                                                                                                    >  2:3933790/1‑150 (MQ=255)
                                  tGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTa                                                                                                                <  2:755917/150‑1 (MQ=255)
                                     cGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTg                                                                                                             <  1:3316771/150‑1 (MQ=255)
                                     cGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTg                                                                                                             <  1:307658/150‑1 (MQ=255)
                                     cGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTg                                                                                                             >  2:3624866/1‑150 (MQ=255)
                                       cTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTgcg                                                                                                           >  2:2040625/1‑150 (MQ=255)
                                          atgatCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCa                                                                                                        <  2:3851223/150‑1 (MQ=255)
                                           tgatCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCag                                                                                                       <  2:372184/150‑1 (MQ=255)
                                              tCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAgg                                                                                                    <  1:213145/150‑1 (MQ=255)
                                                aaCCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGAt                                                                                                  >  1:719389/1‑150 (MQ=255)
                                                aaCCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGAt                                                                                                  >  1:3462546/1‑150 (MQ=255)
                                                aaCCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGAt                                                                                                  <  1:2051316/150‑1 (MQ=255)
                                                 aCCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGAtt                                                                                                 <  1:3756406/150‑1 (MQ=255)
                                                 aCCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGAtt                                                                                                 >  2:4294348/1‑150 (MQ=255)
                                                 aCCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGAtt                                                                                                 >  1:1973451/1‑150 (MQ=255)
                                                  cccGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTa                                                                                                >  1:2772141/1‑150 (MQ=255)
                                                   ccGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTAt                                                                                               <  1:451851/150‑1 (MQ=255)
                                                        aaCCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGAt                                                                                          >  2:390729/1‑150 (MQ=255)
                                                         aCCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGAtt                                                                                         <  2:3318619/150‑1 (MQ=255)
                                                         aCCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGAtt                                                                                         >  2:4074578/1‑150 (MQ=255)
                                                         aCCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGAtt                                                                                         >  1:3552437/1‑150 (MQ=255)
                                                          ccGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTg                                                                                        <  2:537663/150‑1 (MQ=255)
                                                          ccGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTg                                                                                        <  2:2611489/150‑1 (MQ=255)
                                                          ccGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTg                                                                                        <  2:222641/150‑1 (MQ=255)
                                                          ccGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTg                                                                                        >  2:2718218/1‑150 (MQ=255)
                                                            gggCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGtc                                                                                      <  1:3593596/150‑1 (MQ=255)
                                                             ggCGTGTGCATCCCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGtct                                                                                     <  2:4224072/150‑1 (MQ=255)
                                                             ggCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGtct                                                                                     >  2:1360184/1‑150 (MQ=255)
                                                              gCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGtctc                                                                                    <  2:806139/150‑1 (MQ=255)
                                                                 tgtgCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCCCAGc                                                                                 <  1:2335195/150‑1 (MQ=255)
                                                                  gtgCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCg                                                                                >  1:3803376/1‑150 (MQ=255)
                                                                    gCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGa                                                                              <  1:2399810/150‑1 (MQ=255)
                                                                     cATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGac                                                                             <  1:1023120/150‑1 (MQ=255)
                                                                       tACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGacta                                                                           <  1:2738042/150‑1 (MQ=255)
                                                                       tACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGacta                                                                           >  1:3394473/1‑150 (MQ=255)
                                                                        aCCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGactac                                                                          >  2:3220833/1‑150 (MQ=255)
                                                                         ccTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGactact                                                                         >  2:1269806/1‑150 (MQ=255)
                                                                             ttATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAAAGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCa                                                                     >  2:162899/1‑150 (MQ=255)
                                                                              tATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAg                                                                    <  2:1314490/150‑1 (MQ=255)
                                                                               aTCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGa                                                                   <  1:2825974/150‑1 (MQ=255)
                                                                                tCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGAt                                                                  >  1:1875849/1‑150 (MQ=255)
                                                                                 cACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTACCGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGAtt                                                                 >  2:1055434/1‑150 (MQ=255)
                                                                                 cACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGAtt                                                                 <  1:1699389/150‑1 (MQ=255)
                                                                                  aCCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTg                                                                <  2:2543329/150‑1 (MQ=255)
                                                                                  aCCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTg                                                                <  2:2448520/150‑1 (MQ=255)
                                                                                            aaCTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCAt                                                      >  2:1857029/1‑150 (MQ=255)
                                                                                              cTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTa                                                    <  1:326392/150‑1 (MQ=255)
                                                                                              cTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTa                                                    <  2:3608962/150‑1 (MQ=255)
                                                                                                  aaCGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGgc                                                <  1:2526217/150‑1 (MQ=255)
                                                                                                   aCGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGGAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGgcg                                               <  1:4004946/150‑1 (MQ=255)
                                                                                                   aCGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGgcg                                               <  2:606341/150‑1 (MQ=255)
                                                                                                      ggACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCAt                                            <  1:1839288/150‑1 (MQ=255)
                                                                                                       gACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATc                                           <  1:1229114/150‑1 (MQ=255)
                                                                                                         cGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCtt                                         >  1:548602/1‑150 (MQ=255)
                                                                                                          gTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCttt                                        <  2:2958049/150‑1 (MQ=255)
                                                                                                           tttATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTc                                       <  2:375781/150‑1 (MQ=255)
                                                                                                            ttATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTcg                                      >  2:2801772/1‑150 (MQ=255)
                                                                                                            ttATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTcg                                      <  1:390729/150‑1 (MQ=255)
                                                                                                                   cAACCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACa                               >  1:2881112/1‑150 (MQ=255)
                                                                                                                    aaCCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACaa                              >  2:149975/1‑150 (MQ=255)
                                                                                                                    aaCCGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACaa                              >  1:3016046/1‑150 (MQ=255)
                                                                                                                       cGATCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAgg                           <  1:4125786/150‑1 (MQ=255)
                                                                                                                          tCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCtt                        >  2:1408814/1‑150 (MQ=255)
                                                                                                                          tCCTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCtt                        <  2:3372361/150‑1 (MQ=255)
                                                                                                                           ccTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTg                       >  2:117561/1‑150 (MQ=255)
                                                                                                                           ccTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTg                       >  1:1267124/1‑150 (MQ=255)
                                                                                                                            cTAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGc                      >  2:1219103/1‑150 (MQ=255)
                                                                                                                             tAACCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCt                     <  2:3487463/150‑1 (MQ=255)
                                                                                                                              aaCCTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTg                    >  1:2219883/1‑150 (MQ=255)
                                                                                                                               aCCTGCAAAACATTCCGGTGAGTAACGAAGAAGGGCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGGGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTGa                   >  2:1749989/1‑150 (MQ=255)
                                                                                                                                 cTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTGAcc                 >  1:1534008/1‑150 (MQ=255)
                                                                                                                                 cTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTGAcc                 <  2:284968/150‑1 (MQ=255)
                                                                                                                                 cTGCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTGAcc                 <  1:1163112/150‑1 (MQ=255)
                                                                                                                                   gCAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTGACCGc               >  2:1000282/1‑150 (MQ=255)
                                                                                                                                    cAAAACATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTGACCGCa              >  1:1354411/1‑150 (MQ=255)
                                                                                                                                     aaaaCATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTGACCGCAt             >  1:2631922/1‑150 (MQ=255)
                                                                                                                                     aaaaCATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTGACCGCAt             >  1:2680043/1‑150 (MQ=255)
                                                                                                                                      aaaCATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTGACCGCAtt            >  1:3976165/1‑150 (MQ=255)
                                                                                                                                        aCATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTGACCGCATTcg          <  2:3048694/150‑1 (MQ=255)
                                                                                                                                         cATTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTGACCGCATTcgc         >  1:1799025/1‑150 (MQ=255)
                                                                                                                                          aTTCCGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTGACCGCATTcgcg        >  2:1950433/1‑150 (MQ=255)
                                                                                                                                             ccGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTGACCGCATTCGCggaa     <  2:2384702/150‑1 (MQ=255)
                                                                                                                                              cGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTGACCGCATTCGCggaag    >  1:4173029/1‑150 (MQ=255)
                                                                                                                                              cGGTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTGACCGCATTCGCggaag    >  1:1427376/1‑150 (MQ=255)
                                                                                                                                               ggTGAGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTGACCGCATTCGCggaagg   <  2:2318473/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTGGCGAAGCTGAAATCGACCTACACCGACAAGCTGCCGCTGATGATCAACCCGAAAACCGGGCGTGTGCATACCTCTTATCACCAGGCAGTAACTGCAACGGGACGTTTATCGTCAACCGATCCTAACCTGCAAAACATTCCGGTGCGTAACGAAGAAGGTCGTCGTATCCGCCAGGCGTTTATTGCGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTGCGCATTATGGCGCATCTTTCGCGTGACAAAGGCTTGCTGACCGCATTCGCGGAAGGA  >  AP012306/3478075‑3478368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑