| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AP012306 | 318,879 | C→G | intergenic (+84/+161) | mak → / ← araJ | manno(fructo)kinase/predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP012306 | 318,879 | 0 | C | G | 98.2% | 134.8 / ‑3.4 | 56 | intergenic (+84/+161) | mak/araJ | manno(fructo)kinase/predicted transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base G (31/24); total (31/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
GAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAAC‑‑CGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTGCTCCGATGCCTGATGCGAC > AP012306/318738‑318934 | gAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATa > 1:1183860/1‑150 (MQ=25)gAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATa > 2:2460575/1‑150 (MQ=25)gAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATa < 2:3559512/150‑1 (MQ=25) aaGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATaa > 2:4177132/1‑150 (MQ=25) aGCACGGTGATTCCATCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAg < 2:1389858/150‑1 (MQ=21) aGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAg > 1:1068287/1‑150 (MQ=25) aGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAg > 2:3919399/1‑150 (MQ=25) aGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAg < 1:2143977/150‑1 (MQ=25) cACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGc > 1:927389/1‑150 (MQ=25) cACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGc < 2:2449113/150‑1 (MQ=21) aCGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCg > 1:1677722/1‑150 (MQ=25) aCGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCg > 1:449245/1‑150 (MQ=25) cGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGt > 2:3927270/1‑150 (MQ=21) ggTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGtt > 1:4033209/1‑150 (MQ=21) gTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTc > 2:2849698/1‑150 (MQ=21) tGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCa > 1:2255391/1‑150 (MQ=21) tGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCa > 2:4279452/1‑150 (MQ=21) tGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCa < 1:1007218/150‑1 (MQ=21) tGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCa < 1:4218230/150‑1 (MQ=21) aTTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCAcg > 2:1658420/1‑150 (MQ=21) ttCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCAcgc < 1:2261836/150‑1 (MQ=21) tCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCAcgcc < 1:1238208/150‑1 (MQ=21) ccAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCAcgccg < 2:76302/150‑1 (MQ=21) ccAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCAcgccg < 1:590410/150‑1 (MQ=21) cAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCAcgccgc > 2:1044179/1‑150 (MQ=21) cAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCAcgccgc > 2:2395884/1‑150 (MQ=21) cAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCAcgccgc < 2:2424510/150‑1 (MQ=21) aGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCa < 1:65822/150‑1 (MQ=21) aGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCa > 2:3052505/1‑150 (MQ=21) aGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCa > 2:1751809/1‑150 (MQ=21) ggcgTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATcc > 2:836315/1‑150 (MQ=18) ggcgTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATcc > 2:3525472/1‑150 (MQ=18) gcgTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCg > 1:670255/1‑150 (MQ=18) gcgTACGCGGCCTGGCGTGTTTTTGGCCACAAGAGTAAAAAAGGTAGGAAATGGGCGATTAATGCTTGATGCGACGTTTGCCGCGTCTTACCAGGCCTGCAAAAGGTGCCAGAACCGCGTAGGGCGGATAGGGCGTTCACGCCGCATCCg < 2:2896046/150‑1 (MQ=9) cgTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCgg < 1:2693247/150‑1 (MQ=18) cgTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCgg < 2:3725669/150‑1 (MQ=18) gTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGc > 2:265484/1‑150 (MQ=18) tACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCa < 2:2730831/150‑1 (MQ=18) cgcgGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAAt > 1:1718982/1‑150 (MQ=18) cgcgGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAAt > 1:2661267/1‑150 (MQ=18) gcggcgCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGACAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATa < 1:3050829/150‑1 (MQ=17) cggcgCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATaa < 1:3364160/150‑1 (MQ=18) cggcgCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATaa < 1:1517299/150‑1 (MQ=17) ggcgCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAg < 1:2752871/150‑1 (MQ=17) ggcgCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAg < 1:1080375/150‑1 (MQ=17) gcgcTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGt < 1:2755962/150‑1 (MQ=17) cgcTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGta > 2:2538109/1‑149 (MQ=17) cgcTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGta < 2:657878/150‑2 (MQ=17) gcTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGtaa > 1:2214070/1‑148 (MQ=17) gCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGtaatga > 1:2236075/1‑145 (MQ=17) cGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGtaatgag > 2:3558028/1‑144 (MQ=14) gTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGtaatgagc > 2:1658378/1‑143 (MQ=14) gTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGtaatgagc > 1:1409631/1‑143 (MQ=14) gTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGtaatgagc < 2:434297/150‑8 (MQ=14) gTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGtaatgagc > 2:168586/1‑143 (MQ=14) cacaAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAAC‑‑CGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTGCTCCGATGCCTGATGCGAc < 1:3385242/150‑1 (MQ=255) | GAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAAGGTGCCAGAAC‑‑CGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTGCTCCGATGCCTGATGCGAC > AP012306/318738‑318934 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |