BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	1,063,716	C→T	A76T (GCG→ACG)	pth ←	peptidyl‑tRNA hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	1,063,716	0	C	T	100.0%	1215.3 / NA	294	A76T (GCG→ACG)	pth	peptidyl‑tRNA hydrolase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (146/148); total (146/148)

Only 100 of 306 total aligned reads displayed.

CCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGA  >  AP012306/1063569‑1063862

 cAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAAc                                                                                                                                                 <  1:927173/150‑1 (MQ=255)

  aaTTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACg                                                                                                                                                <  1:4214974/150‑1 (MQ=255)

     ttACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCt                                                                                                                                             <  1:564722/150‑1 (MQ=255)

     ttACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCt                                                                                                                                             >  2:2190404/1‑150 (MQ=255)

      tACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCtt                                                                                                                                            >  2:4211279/1‑150 (MQ=255)

       aCTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCttt                                                                                                                                           <  1:3865937/150‑1 (MQ=255)

       aCTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCttt                                                                                                                                           <  2:1684829/150‑1 (MQ=255)

        cTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTg                                                                                                                                          >  2:3267712/1‑150 (MQ=255)

         tgatgaTGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGc                                                                                                                                         <  2:2716393/150‑1 (MQ=255)

          gatgatGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGcc                                                                                                                                        >  2:569015/1‑150 (MQ=255)

              atgTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTg                                                                                                                                    >  1:2736279/1‑150 (MQ=255)

                gTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTgag                                                                                                                                  <  2:3791054/150‑1 (MQ=255)

                gTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTgag                                                                                                                                  >  1:1206737/1‑150 (MQ=255)

                 tCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTgaga                                                                                                                                 <  2:1137838/150‑1 (MQ=255)

                     tCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCa                                                                                                                             >  1:945522/1‑150 (MQ=255)

                     tCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCa                                                                                                                             >  2:3429903/1‑150 (MQ=255)

                      cAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCAt                                                                                                                            <  1:809524/150‑1 (MQ=255)

                      cAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCAt                                                                                                                            >  2:1818078/1‑150 (MQ=255)

                      cAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCAt                                                                                                                            <  1:2811163/150‑1 (MQ=255)

                        gTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATaa                                                                                                                          >  2:1753372/1‑150 (MQ=255)

                         tCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATaaa                                                                                                                         <  1:4265491/150‑1 (MQ=255)

                           cATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATg                                                                                                                       <  2:2766988/150‑1 (MQ=255)

                             ttGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTa                                                                                                                     <  2:1461563/150‑1 (MQ=255)

                              tgtgACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAg                                                                                                                    >  1:4060516/1‑150 (MQ=255)

                              tgtgACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAg                                                                                                                    >  1:3635307/1‑150 (MQ=255)

                                 gACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCg                                                                                                                 <  1:4001330/150‑1 (MQ=255)

                                  accaccATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCgg                                                                                                                <  1:786902/150‑1 (MQ=255)

                                   ccaccaTGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCggg                                                                                                               >  1:1074007/1‑150 (MQ=255)

                                   ccaccaTGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCggg                                                                                                               <  2:2385912/150‑1 (MQ=255)

                                     accaTGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGAc                                                                                                             <  1:2012276/150‑1 (MQ=255)

                                     accaTGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGAc                                                                                                             >  2:504450/1‑150 (MQ=255)

                                      ccaTGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACt                                                                                                            <  1:1152041/150‑1 (MQ=255)

                                      ccaTGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACt                                                                                                            >  2:3361153/1‑150 (MQ=255)

                                       caTGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTa                                                                                                           >  2:2628593/1‑150 (MQ=255)

                                        aTGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTaa                                                                                                          <  1:1032724/150‑1 (MQ=255)

                                         tGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAAc                                                                                                         <  1:4154814/150‑1 (MQ=255)

                                          ggCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACa                                                                                                        <  1:3770943/150‑1 (MQ=255)

                                          ggCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACa                                                                                                        <  2:1376154/150‑1 (MQ=255)

                                            ccACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAgg                                                                                                      <  1:733360/150‑1 (MQ=255)

                                             cACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGc                                                                                                     <  1:1778356/150‑1 (MQ=255)

                                                 gCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGAc                                                                                                 <  2:850743/150‑1 (MQ=255)

                                                    cAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATc                                                                                              <  1:3753832/150‑1 (MQ=255)

                                                        ttAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCg                                                                                          >  2:3532374/1‑150 (MQ=255)

                                                          aaaTTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGcc                                                                                        >  1:2995143/1‑150 (MQ=255)

                                                          aaaTTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGcc                                                                                        >  1:3193736/1‑150 (MQ=255)

                                                           aaTTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCt                                                                                       >  1:1318926/1‑150 (MQ=255)

                                                           aaTTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCt                                                                                       <  2:473122/150‑1 (MQ=255)

                                                            aTTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTc                                                                                      >  2:2973509/1‑150 (MQ=255)

                                                            aTTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTc                                                                                      <  1:2504966/150‑1 (MQ=255)

                                                             tttGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTcc                                                                                     >  2:3404279/1‑150 (MQ=255)

                                                             tttGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTcc                                                                                     >  2:2907947/1‑150 (MQ=255)

                                                              ttGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCa                                                                                    <  1:150451/150‑1 (MQ=255)

                                                                ggCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAag                                                                                  <  2:1031343/150‑1 (MQ=255)

                                                                ggCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAag                                                                                  <  1:2046280/150‑1 (MQ=255)

                                                                   gACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGt                                                                               <  2:200348/150‑1 (MQ=255)

                                                                   gACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGt                                                                               >  1:3312480/1‑150 (MQ=255)

                                                                    aCGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTg                                                                              >  1:1280033/1‑150 (MQ=255)

                                                                    aCGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTg                                                                              <  2:4231008/150‑1 (MQ=255)

                                                                       ccAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACt                                                                           >  2:1613971/1‑150 (MQ=255)

                                                                         aggaggCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTcg                                                                         >  2:4051378/1‑150 (MQ=255)

                                                                           gaggCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTcgcg                                                                       <  1:2550264/150‑1 (MQ=255)

                                                                             ggCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGaa                                                                     >  2:3590789/1‑150 (MQ=255)

                                                                              gCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAg                                                                    >  1:3376270/1‑150 (MQ=255)

                                                                               cAGATCCAGTTCGTCGTGTGCCACCAGAATTTCGGCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAgg                                                                   >  2:2462975/1‑149 (MQ=255)

                                                                                aGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGta                                                                  <  1:47060/150‑1 (MQ=255)

                                                                                aGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGta                                                                  >  2:300935/1‑150 (MQ=255)

                                                                                 gATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGtat                                                                 <  1:4016251/150‑1 (MQ=255)

                                                                                  aTCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGtata                                                                <  2:3692860/150‑1 (MQ=255)

                                                                                  aTCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGtata                                                                <  1:381246/150‑1 (MQ=255)

                                                                                  aTCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGtata                                                                >  2:3851895/1‑150 (MQ=255)

                                                                                    ccAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAAc                                                              <  1:3398008/150‑1 (MQ=255)

                                                                                              gtGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAAttt                                                    <  1:1964286/150‑1 (MQ=255)

                                                                                               tGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTa                                                   <  2:2124305/150‑1 (MQ=255)

                                                                                               tGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTa                                                   <  1:2975260/150‑1 (MQ=255)

                                                                                                    caccaGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCctc                                              <  1:3728575/150‑1 (MQ=255)

                                                                                                     accaGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCctct                                             <  2:3067214/150‑1 (MQ=255)

                                                                                                      ccaGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCtt                                            <  1:1740523/150‑1 (MQ=255)

                                                                                                      ccaAAATTTCGTCCGGATAAATGCGAAAAAACCGGCCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACAAACAGGCGGACATTTTCCCCTCAAAGAGTGACTCGCGAAGTAAAACAAAAAAATTTAGCCTCtt                                            <  2:316477/150‑1 (MQ=255)

                                                                                                       caGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTc                                           <  2:2808554/150‑1 (MQ=255)

                                                                                                       caGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTc                                           >  2:3194455/1‑150 (MQ=255)

                                                                                                        aGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTcg                                          <  2:2274635/150‑1 (MQ=255)

                                                                                                         gAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTcgc                                         <  1:593363/150‑1 (MQ=255)

                                                                                                           aTTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCgcgc                                       <  2:1925668/150‑1 (MQ=255)

                                                                                                             ttCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAg                                     <  2:362786/150‑1 (MQ=255)

                                                                                                                gTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCgg                                  >  2:1221274/1‑150 (MQ=255)

                                                                                                                 tCCGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGa                                 >  2:3037278/1‑150 (MQ=255)

                                                                                                                   cGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAgc                               >  2:804986/1‑150 (MQ=255)

                                                                                                                   cGGATTAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAgc                               <  2:661025/150‑1 (MQ=255)

                                                                                                                   cGGATTAATGCGGAAAAAACTGGCCATCGACGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAgc                               >  2:3931496/1‑150 (MQ=255)

                                                                                                                        tAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCa                          >  1:309003/1‑150 (MQ=255)

                                                                                                                        tAATGCGGAAAAAACTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCa                          >  1:1669693/1‑150 (MQ=255)

                                                                                                                                aaaaaaCTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGctct                  >  1:2183232/1‑150 (MQ=255)

                                                                                                                                 aaaaaCTGGCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTg                 >  2:4138743/1‑150 (MQ=255)

                                                                                                                                        ggCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTaa          >  2:942120/1‑150 (MQ=255)

                                                                                                                                         gCCATCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAg         >  2:1105052/1‑150 (MQ=255)

                                                                                                                                            aTCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCa      >  1:241264/1‑150 (MQ=255)

                                                                                                                                             tCGCCGTAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCaa     >  1:211680/1‑150 (MQ=255)

                                                                                                                                               gccgtAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACg   >  1:969513/1‑150 (MQ=255)

                                                                                                                                               gccgtAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACg   <  1:110122/150‑1 (MQ=255)

                                                                                                                                                ccgtAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGa  >  2:1882643/1‑150 (MQ=255)

CCAATTTACTGATGATGTCTTTCAGTCCATTGTGACCACCATGGCCACCGCCCAATTTAAATTTGGCGACGCCAGGAGGCAGATCCAGTTCGTCGTGGGCCACCAGAATTTCGTCCGGATTAATGCGGAAAAAACTGGCCATCGCCGCAACGGCTTTGCCGCTGAGATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGA  >  AP012306/1063569‑1063862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`