BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	1,093,310	C→A	R248L (CGC→CTC)	purU ←	formyltetrahydrofolate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	1,093,310	0	C	A	100.0%	1671.2 / NA	412	R248L (CGC→CTC)	purU	formyltetrahydrofolate hydrolase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (213/199); total (213/199)

Only 100 of 416 total aligned reads displayed.

TTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGT  >  AP012306/1093163‑1093456

  ttCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGTACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGcat                                                                                                                                                <  1:1610092/150‑1 (MQ=255)

  ttCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGcat                                                                                                                                                <  1:3109850/150‑1 (MQ=255)

  ttCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGcat                                                                                                                                                <  2:2111337/150‑1 (MQ=255)

  ttCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGcat                                                                                                                                                <  2:1342560/150‑1 (MQ=255)

     aTCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGcatcat                                                                                                                                             >  1:2557513/1‑150 (MQ=255)

      tCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCata                                                                                                                                            <  2:2303923/150‑1 (MQ=255)

      tCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCata                                                                                                                                            >  2:3468764/1‑150 (MQ=255)

       ccGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCatat                                                                                                                                           <  1:3487862/150‑1 (MQ=255)

       ccGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCatat                                                                                                                                           >  2:1474555/1‑150 (MQ=255)

       ccGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCatat                                                                                                                                           <  2:1408782/150‑1 (MQ=255)

           aaCGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTc                                                                                                                                       >  1:3601440/1‑150 (MQ=255)

            aCGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCa                                                                                                                                      <  1:1809488/150‑1 (MQ=255)

               gATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCt                                                                                                                                   <  1:1076165/150‑1 (MQ=255)

                aTTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCtg                                                                                                                                  >  1:3221742/1‑150 (MQ=255)

                 ttAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCtgt                                                                                                                                 >  1:165664/1‑150 (MQ=255)

                     aGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAg                                                                                                                             <  1:2270071/150‑1 (MQ=255)

                       gTAACCAGTTATTTTTGCTGGCGATTAAAGAATAGTCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGt                                                                                                                           >  2:3329904/1‑150 (MQ=255)

                       gTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGt                                                                                                                           <  2:1933092/150‑1 (MQ=255)

                         aaCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTAt                                                                                                                         >  1:1471263/1‑150 (MQ=255)

                          aCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATg                                                                                                                        <  1:328430/150‑1 (MQ=255)

                          aCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTAATTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATg                                                                                                                        >  2:3738028/1‑150 (MQ=255)

                            cAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGAt                                                                                                                      <  1:3417727/150‑1 (MQ=255)

                             aGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATc                                                                                                                     <  2:3162362/150‑1 (MQ=255)

                              gTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCg                                                                                                                    >  2:1116622/1‑150 (MQ=255)

                              gTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCg                                                                                                                    >  1:4125718/1‑150 (MQ=255)

                                 aTTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACa                                                                                                                 >  2:3599669/1‑150 (MQ=255)

                                  tttttGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACAt                                                                                                                <  1:4273337/150‑1 (MQ=255)

                                  tttttGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACAt                                                                                                                <  2:2472515/150‑1 (MQ=255)

                                   ttttGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATg                                                                                                               <  2:2894570/150‑1 (MQ=255)

                                   ttttGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATg                                                                                                               >  1:3934698/1‑150 (MQ=255)

                                      tGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAAt                                                                                                            >  2:704195/1‑150 (MQ=255)

                                         tGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAAc                                                                                                         <  1:3388870/150‑1 (MQ=255)

                                            cGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTc                                                                                                      >  1:3198656/1‑150 (MQ=255)

                                              aTTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCt                                                                                                    <  1:882683/150‑1 (MQ=255)

                                              aTTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCt                                                                                                    >  1:3880321/1‑150 (MQ=255)

                                               ttAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCTAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTg                                                                                                   >  1:1169097/1‑150 (MQ=255)

                                               ttAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTg                                                                                                   <  1:2912096/150‑1 (MQ=255)

                                                  aaGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCat                                                                                                <  2:1639855/150‑1 (MQ=255)

                                                   aGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCatg                                                                                               >  1:2870847/1‑150 (MQ=255)

                                                    gAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCatga                                                                                              >  2:2624542/1‑150 (MQ=255)

                                                      ataatCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCatgatg                                                                                            >  1:3347855/1‑150 (MQ=255)

                                                       taatCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCAtgatga                                                                                           >  2:2553817/1‑150 (MQ=255)

                                                        aatCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATgatgat                                                                                          >  2:4285263/1‑150 (MQ=255)

                                                        aatCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATgatgat                                                                                          >  1:1899757/1‑150 (MQ=255)

                                                         atCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGAtt                                                                                         <  1:2956347/150‑1 (MQ=255)

                                                           cGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTgg                                                                                       <  1:402333/150‑1 (MQ=255)

                                                           cGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTgg                                                                                       <  2:1227474/150‑1 (MQ=255)

                                                            gTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTggg                                                                                      >  1:2786425/1‑150 (MQ=255)

                                                            gTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTggg                                                                                      >  1:3047450/1‑150 (MQ=255)

                                                                gATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCtt                                                                                  >  2:3820334/1‑150 (MQ=255)

                                                                gATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCtt                                                                                  >  2:4055334/1‑150 (MQ=255)

                                                                gATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCtt                                                                                  >  2:1762009/1‑150 (MQ=255)

                                                                gATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCtt                                                                                  >  2:489402/1‑150 (MQ=255)

                                                                   tACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGt                                                                               <  1:672166/150‑1 (MQ=255)

                                                                    aCCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTc                                                                              <  2:596556/150‑1 (MQ=255)

                                                                       gTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAg                                                                           <  2:2786425/150‑1 (MQ=255)

                                                                       gTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAg                                                                           >  1:483687/1‑150 (MQ=255)

                                                                          aaCAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGAtt                                                                        >  2:524709/1‑150 (MQ=255)

                                                                            cAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGTCTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGt                                                                      >  1:1548178/1‑150 (MQ=255)

                                                                            cAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGt                                                                      >  2:4221439/1‑150 (MQ=255)

                                                                            cAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGt                                                                      <  1:860405/150‑1 (MQ=255)

                                                                             aaaGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTc                                                                     <  2:2360167/150‑1 (MQ=255)

                                                                              aaGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCa                                                                    >  2:3992957/1‑150 (MQ=255)

                                                                               aGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCAt                                                                   >  1:61617/1‑150 (MQ=255)

                                                                                   gcgcTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTcac                                                               <  2:1284528/150‑1 (MQ=255)

                                                                                    cgcTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTTCAGATTGTCATTcaca                                                              >  1:4149534/1‑150 (MQ=255)

                                                                                    cgcTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTcaca                                                              <  1:2500524/150‑1 (MQ=255)

                                                                                      cTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATa                                                            >  1:2605540/1‑150 (MQ=255)

                                                                                      cTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATa                                                            <  1:1022741/150‑1 (MQ=255)

                                                                                        gtgCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGt                                                          >  1:120651/1‑150 (MQ=255)

                                                                                          gCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGa                                                        <  1:3728112/150‑1 (MQ=255)

                                                                                            cAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGc                                                      <  2:1819643/150‑1 (MQ=255)

                                                                                            cAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGc                                                      <  2:2703508/150‑1 (MQ=255)

                                                                                              gTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGCGCCTTCGTCCAGATTGTCATTCACATAGTGAGcag                                                    >  2:63743/1‑148 (MQ=255)

                                                                                               tACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGt                                                   <  1:1245017/150‑1 (MQ=255)

                                                                                                  tttGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTgc                                                >  2:826687/1‑150 (MQ=255)

                                                                                                   ttGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTgcg                                               <  1:838263/150‑1 (MQ=255)

                                                                                                    tGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTgcgc                                              >  1:1347442/1‑150 (MQ=255)

                                                                                                     gTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGcc                                             >  1:1763262/1‑150 (MQ=255)

                                                                                                      tataGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCa                                            <  1:2704696/150‑1 (MQ=255)

                                                                                                      tataGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCa                                            >  2:3032500/1‑150 (MQ=255)

                                                                                                          gTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCaataa                                        <  2:785448/150‑1 (MQ=255)

                                                                                                            gCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATc                                      >  2:2021315/1‑150 (MQ=255)

                                                                                                            gCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATc                                      <  2:2956109/150‑1 (MQ=255)

                                                                                                              aCGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCtt                                    <  2:1006017/150‑1 (MQ=255)

                                                                                                               cGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTc                                   >  2:680886/1‑150 (MQ=255)

                                                                                                                      aGACGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGATATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCAcaccac                            <  1:1852466/150‑1 (MQ=255)

                                                                                                                         cGTTTTTCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGtt                         <  1:2160806/150‑1 (MQ=255)

                                                                                                                           tttttCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCa                       >  2:2195255/1‑150 (MQ=255)

                                                                                                                            ttttCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCAt                      <  2:3067499/150‑1 (MQ=255)

                                                                                                                            ttttCTCGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCAt                      <  1:4222966/150‑1 (MQ=255)

                                                                                                                                 tcGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGcc                 <  1:223576/150‑1 (MQ=255)

                                                                                                                                  cGACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCt                <  1:1978795/150‑1 (MQ=255)

                                                                                                                                   gACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTg               <  2:4146786/150‑1 (MQ=255)

                                                                                                                                   gACGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTg               >  2:320333/1‑150 (MQ=255)

                                                                                                                                     cGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGt             <  1:241121/150‑1 (MQ=255)

                                                                                                                                     cGTCACGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGt             >  2:3956551/1‑150 (MQ=255)

                                                                                                                                          cGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATaa        <  1:2071397/150‑1 (MQ=255)

                                                                                                                                          cGACCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATaa        >  2:3263151/1‑150 (MQ=255)

                                                                                                                                            aCCTGCGAGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAgg      <  1:471696/150‑1 (MQ=255)

TTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGTCATTCACATAGTGAGCGGTTGCGCCAATAATCTTCACACCACGTTCATAGGCCTGGTGATAAGGACGT  >  AP012306/1093163‑1093456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`