Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 1,121,980 C→A R222L (CGC→CTC)  trpB ← tryptophan synthase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,121,9800CA100.0% 858.5 / NA 212R222L (CGC→CTC) trpBtryptophan synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (123/89);  total (123/89)

Only 100 of 214 total aligned reads displayed.

AGCGGTGCGCCGTGCTCGCCAGTTTC‑GATACCGTGACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTCGTAACTACCGGACCAGTCGCGCAGC  >  AP012306/1121833‑1122126
                                                                                                                                                    |                                                                                                                                                  
aGCGGTGCGCCGTGCTCGCCAGTTTC‑GATACCGTGACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGgaga                                                                                                                                                  >  1:1343773/1‑150 (MQ=255)
aGCGGTGCGCCGTGCTCGCCAGTTTC‑GATACCGTGACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGgaga                                                                                                                                                  >  2:3751602/1‑150 (MQ=255)
      gcgcCGGGCTCGCC‑GTTTCGGATACCGTACCCCCCTGGCCCCACACCAATAAGGCCGATGTTGGTTTCATAAAGGAACTCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTtct                                                                                                                                            <  2:3261640/150‑1 (MQ=255)
          cGTGCTCCCCATTTTC‑GATCCCGTGCCCCCCGGGCTCCACCCAAATCAGGCCGACGTGGGTTTCATTGATAAAACCAGAAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCttt                                                                                                                                        <  2:3802964/150‑1 (MQ=255)
           gTGCTCGCCAGTTTC‑GATACCGTGACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTc                                                                                                                                       >  2:856365/1‑150 (MQ=255)
            tGCTCGCCAGTTTC‑GATACCGTGACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTcc                                                                                                                                      >  2:1903224/1‑150 (MQ=255)
             gCTCGCCAGTTTC‑GATACCGTGACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATTGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCa                                                                                                                                     >  1:4267071/1‑150 (MQ=255)
                 gCCATTTTC‑GATACCGTGACCCCTTGCCCCCCCCCCAATCGGGCCGACGTGGGTTTCATTGATAAAATCGGAAATCATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAAt                                                                                                                                 <  2:212746/150‑1 (MQ=255)
                 gCCATTTTC‑GAACCCGTGCCCCCCTGGCCCCACCCCAATCAGCCCGACTTTGGTTTCATTAATAAAACCAGAAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAAt                                                                                                                                 <  2:2624128/150‑1 (MQ=255)
                    aGTTTC‑GATCCCGTGCCCCCCTGCCTCCCCACAAACCAGGCCGCCGTTGTTTTCTTTGATAAAATCAGAAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTg                                                                                                                              <  2:2325034/150‑1 (MQ=255)
                    aGTTTC‑GATACCGTGACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTg                                                                                                                              >  1:3697310/1‑150 (MQ=255)
                     gTTTC‑GATACCGTGACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTgc                                                                                                                             >  1:3832956/1‑150 (MQ=255)
                        tC‑GATCCCGTGCCCCCCGGGCTCCACACCAATCGGGCCGACGTTGGTTTCATTGAGAAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCt                                                                                                                          <  2:2557152/150‑1 (MQ=255)
                             tACCGTGACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTgg                                                                                                                      >  2:3408564/1‑150 (MQ=255)
                              aCCGTGACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGt                                                                                                                     >  2:1402032/1‑150 (MQ=255)
                                 gTGACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTttc                                                                                                                  >  1:2929184/1‑150 (MQ=255)
                                   gACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTttctt                                                                                                                >  1:1658248/1‑150 (MQ=255)
                                   gACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTttctt                                                                                                                >  2:3123825/1‑150 (MQ=255)
                                    ccccccgggCCCCCCACAAACCGGGCGGGCGTTGTTTTAATTGATAAAACCAGAAACCATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTttcttc                                                                                                               <  2:1935732/143‑1 (MQ=255)
                                       accTGGCTCCACACCAATCAGGCCTACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGcc                                                                                                            >  1:81263/1‑150 (MQ=255)
                                        ccTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCa                                                                                                           >  2:2490916/1‑150 (MQ=255)
                                        ccTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCa                                                                                                           >  1:1882078/1‑150 (MQ=255)
                                            gCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAatca                                                                                                       >  1:3163743/1‑150 (MQ=255)
                                               ccACACCAATCAGGCCGACGTGGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATcc                                                                                                    <  1:3637837/150‑1 (MQ=255)
                                                caccCCAATCAGGCCGACGTTGGTTTAATTGATGAAATCAGAAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCATGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCg                                                                                                   <  2:1341672/150‑1 (MQ=255)
                                                cacacaAATCAGGCCAATGTGGTTTTAATTAAAAAAATCAGAAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCg                                                                                                   <  2:2066873/150‑1 (MQ=255)
                                                cacacaAATCAGCCCGACGTTGTTTTCATGAATAAAATCAGAAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCg                                                                                                   <  2:2306036/150‑1 (MQ=255)
                                                   acCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTg                                                                                                >  2:1734658/1‑150 (MQ=255)
                                                     aaaTCAGCCCGACGTTGGTTTCATTGATGAAATCAGAAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGaa                                                                                              <  1:2328684/149‑1 (MQ=255)
                                                       aTCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAAc                                                                                            >  2:2034615/1‑150 (MQ=255)
                                                       aTCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAAc                                                                                            >  1:3247624/1‑150 (MQ=255)
                                                            gCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACg                                                                                       >  2:4196830/1‑150 (MQ=255)
                                                            gCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACg                                                                                       >  1:2813030/1‑150 (MQ=255)
                                                             ccGACGTTGTTTTCATTGATAAAATCAGAAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGc                                                                                      <  2:3547391/150‑1 (MQ=255)
                                                                aCGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGcaca                                                                                   >  2:1962871/1‑150 (MQ=255)
                                                                 cGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa                                                                                  >  2:756991/1‑150 (MQ=255)
                                                                 cGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACaa                                                                                  >  2:1041807/1‑150 (MQ=255)
                                                                  gttggttgCGTTGAGGAAACCAGAAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAAt                                                                                 <  1:745698/150‑1 (MQ=255)
                                                                    tGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATgg                                                                               >  2:2734308/1‑150 (MQ=255)
                                                                     ggTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGt                                                                              >  2:3427243/1‑150 (MQ=255)
                                                                     ggTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGt                                                                              >  1:3738130/1‑150 (MQ=255)
                                                                     ggTTTCATTGATAAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGt                                                                              <  2:1545075/150‑1 (MQ=255)
                                                                      gTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTc                                                                             >  1:507292/1‑150 (MQ=255)
                                                                       tttatttGATGAAATCAGCAAAAATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCg                                                                            <  2:2390802/145‑1 (MQ=255)
                                                                        ttCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCgg                                                                           >  1:1693309/1‑150 (MQ=255)
                                                                          cATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGAt                                                                         >  1:3914698/1‑150 (MQ=255)
                                                                             tgatgaAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAg                                                                      >  2:2991585/1‑150 (MQ=255)
                                                                              gatgaAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAgg                                                                     >  2:4157026/1‑150 (MQ=255)
                                                                              gatgaAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAgg                                                                     >  1:2750723/1‑150 (MQ=255)
                                                                               atgaAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGa                                                                    >  2:390641/1‑150 (MQ=255)
                                                                               atgaAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGa                                                                    >  2:2273817/1‑150 (MQ=255)
                                                                               atgaAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGa                                                                    >  1:1146052/1‑150 (MQ=255)
                                                                               atgaAATCAGAAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGa                                                                    <  1:2850114/150‑1 (MQ=255)
                                                                               agaaacccAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGa                                                                    <  2:4103218/143‑1 (MQ=255)
                                                                                taaaaTCGGCAACCATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGAt                                                                   <  1:2988243/148‑1 (MQ=255)
                                                                                 gaaaccagaaaccaTGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATg                                                                  <  2:36113/150‑1 (MQ=255)
                                                                                 gaAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATg                                                                  >  1:739597/1‑150 (MQ=255)
                                                                                 aaaaTCAGAAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATg                                                                  <  1:4293645/149‑1 (MQ=255)
                                                                                  aaaTCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGc                                                                 >  2:2856829/1‑150 (MQ=255)
                                                                                   aaTCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCg                                                                >  2:1466201/1‑150 (MQ=255)
                                                                                    aTCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCgg                                                               >  2:1107681/1‑150 (MQ=255)
                                                                                    aTCAGAAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCgg                                                               <  2:3562931/150‑1 (MQ=255)
                                                                                       aGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGcc                                                            >  1:2389761/1‑150 (MQ=255)
                                                                                        gaaaaCATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCa                                                           <  2:2220971/148‑1 (MQ=255)
                                                                                          aaaCATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGc                                                         >  2:3653321/1‑150 (MQ=255)
                                                                                           aaCATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCt                                                        <  2:1092232/150‑1 (MQ=255)
                                                                                           aaCATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCt                                                        <  1:1536087/150‑1 (MQ=255)
                                                                                           aaCATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCt                                                        >  1:2547756/1‑150 (MQ=255)
                                                                                           aaCATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCt                                                        <  1:163588/150‑1 (MQ=255)
                                                                                               tGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCgg                                                    >  2:1805522/1‑150 (MQ=255)
                                                                                                  cGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGc                                                 >  2:3894212/1‑150 (MQ=255)
                                                                                                   gATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGcc                                                >  1:3211860/1‑150 (MQ=255)
                                                                                                    aTGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGccc                                               >  1:1599835/1‑150 (MQ=255)
                                                                                                       gCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGc                                            <  2:1714544/150‑1 (MQ=255)
                                                                                                       gCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGc                                            >  1:2696318/1‑150 (MQ=255)
                                                                                                        cATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCa                                           <  1:57503/150‑1 (MQ=255)
                                                                                                         aTTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTTAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCat                                          >  1:4133441/1‑150 (MQ=255)
                                                                                                          ttCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCata                                         >  1:1087297/1‑150 (MQ=255)
                                                                                                           tCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCatat                                        >  2:2473071/1‑150 (MQ=255)
                                                                                                             gacccgccgCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAg                                      <  2:442332/147‑1 (MQ=255)
                                                                                                              aaCCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGt                                     <  1:3440708/150‑1 (MQ=255)
                                                                                                                     gccAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGt                              <  2:535778/150‑1 (MQ=255)
                                                                                                                       cAACACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGttt                            >  2:1506836/1‑150 (MQ=255)
                                                                                                                        aaCACAGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTc                           >  2:602803/1‑150 (MQ=255)
                                                                                                                             aGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTCGTAAc                      >  1:2905499/1‑150 (MQ=255)
                                                                                                                             aGGCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTCGTAAc                      >  2:1637519/1‑150 (MQ=255)
                                                                                                                              ggCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTCGTAACt                     >  1:226667/1‑150 (MQ=255)
                                                                                                                              ggCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTCGTAACt                     <  1:2436828/150‑1 (MQ=255)
                                                                                                                              ggCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTCGTAACt                     <  1:2653430/150‑1 (MQ=255)
                                                                                                                              ggCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTCGTAACt                     <  2:2586599/150‑1 (MQ=255)
                                                                                                                               gCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTCGTAACTa                    <  1:3791813/150‑1 (MQ=255)
                                                                                                                               gCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTCGTAACTa                    >  2:3360684/1‑150 (MQ=255)
                                                                                                                               gCGATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTCGTAACTa                    >  2:2696333/1‑150 (MQ=255)
                                                                                                                                 gATAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTCGTAACTAcc                  <  2:2103752/150‑1 (MQ=255)
                                                                                                                                  aTAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTCGTAACTACCg                 <  1:3582037/150‑1 (MQ=255)
                                                                                                                                   tAACGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTCGTAACTACCgg                <  2:175573/150‑1 (MQ=255)
                                                                                                                                    aaCGGCATCCGGCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTCGTAACTACCGGa               >  1:1433998/1‑150 (MQ=255)
                                                                                                                                              ggCAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTCGTAACTACCGGACCAGTCgcgc     <  2:3830009/150‑1 (MQ=255)
                                                                                                                                                cAGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTCGTAACTACCGGACCAGTCGCGCAg   <  1:1902243/150‑1 (MQ=255)
                                                                                                                                                 aGGAGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTCGTAACTACCGGACCAGTCGCGCAgc  <  1:1858089/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGCGGTGCGCCGTGCTCGCCAGTTTC‑GATACCGTGACCACCTGGCTCCACACCAATCAGGCCGACGTTGGTTTCATTGATGAAATCAGCAAACATGCCGATGGCATTCGAACCGCCGCCAACACAGGCGATAACGGCATCCGGCAGGCGACCTTCTCTTTCCAGAATCTGCGCTTTGGTTTCTTCGCCAATCATCCGCTGAAACTCACGCACAATGGTCGGATAAGGATGCGGGCCAGCTGCGGTGCCCAGCATATAGTGCGCGGTTTCGTAACTACCGGACCAGTCGCGCAGC  >  AP012306/1121833‑1122126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑