BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	1,273,577	C→T	A18T (GCA→ACA)	yddV ←	predicted diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	1,273,577	0	C	T	100.0%	1152.7 / NA	293	A18T (GCA→ACA)	yddV	predicted diguanylate cyclase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (153/140); total (153/140)

Only 100 of 304 total aligned reads displayed.

CTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGCGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGT  >  AP012306/1273430‑1273723

cttcGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTgtgg                                                                                                                                                  <  1:4210565/150‑1 (MQ=255)

  tcGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTgtggcg                                                                                                                                                <  1:2923526/150‑1 (MQ=255)

       gTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGaata                                                                                                                                           <  1:2177788/150‑1 (MQ=255)

         tttCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGaataat                                                                                                                                         <  1:1159823/150‑1 (MQ=255)

          ttCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATc                                                                                                                                        >  1:2482105/1‑150 (MQ=255)

            ccAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCg                                                                                                                                      >  1:1257704/1‑150 (MQ=255)

            ccAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCg                                                                                                                                      >  1:4138261/1‑150 (MQ=255)

              aGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCgaa                                                                                                                                    >  1:2744077/1‑150 (MQ=255)

                tAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCgaaga                                                                                                                                  >  2:3015505/1‑150 (MQ=255)

                tAAAGAGAAGATACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCgaaga                                                                                                                                  >  2:1050870/1‑150 (MQ=255)

                 aaCGAGAAGATACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCgaagag                                                                                                                                 >  1:2863558/1‑150 (MQ=255)

                 aaCGAGAAGATACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCgaagag                                                                                                                                 >  1:3501145/1‑150 (MQ=255)

                 aaCGAGAAGATACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCgaagag                                                                                                                                 >  2:2948689/1‑150 (MQ=255)

                      gaagaTACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGAt                                                                                                                            >  1:4194395/1‑150 (MQ=255)

                       aagaTACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATc                                                                                                                           >  2:2513887/1‑150 (MQ=255)

                        agaTACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCa                                                                                                                          >  1:1386741/1‑150 (MQ=255)

                        agaTACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCa                                                                                                                          >  1:522260/1‑150 (MQ=255)

                         gaTACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCAc                                                                                                                         >  2:1381118/1‑150 (MQ=255)

                          aTACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCAcc                                                                                                                        <  1:3605243/150‑1 (MQ=255)

                           tACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCg                                                                                                                       >  2:1581109/1‑150 (MQ=255)

                            aCGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCgg                                                                                                                      <  2:2068605/150‑1 (MQ=255)

                            aCGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCgg                                                                                                                      >  2:3366540/1‑150 (MQ=255)

                             cGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGa                                                                                                                     >  2:2846393/1‑150 (MQ=255)

                               aTAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATa                                                                                                                   <  1:1421817/150‑1 (MQ=255)

                                tAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGGTag                                                                                                                  >  1:3983051/1‑150 (MQ=255)

                                tAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATag                                                                                                                  <  1:1016427/150‑1 (MQ=255)

                                 aGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATaga                                                                                                                 >  1:1065325/1‑150 (MQ=255)

                                     ttCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGCAGAGAAGATCACCGGATAGAGGAt                                                                                                             >  1:1738866/1‑150 (MQ=255)

                                       cATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGAttt                                                                                                           <  1:2088508/150‑1 (MQ=255)

                                        aTCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGAtttt                                                                                                          >  2:803442/1‑150 (MQ=255)

                                        aTCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGAtttt                                                                                                          <  1:2302347/150‑1 (MQ=255)

                                           ttCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGAttttttt                                                                                                       >  2:2426382/1‑150 (MQ=255)

                                             ccTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGGTAGAGGATTTTTTTCa                                                                                                     >  1:1647329/1‑150 (MQ=255)

                                              cTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAg                                                                                                    >  1:1768673/1‑150 (MQ=255)

                                              cTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAg                                                                                                    >  2:3831299/1‑150 (MQ=255)

                                                 tGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCAc                                                                                                 >  1:687904/1‑150 (MQ=255)

                                                   aGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCAccc                                                                                               <  1:769767/150‑1 (MQ=255)

                                                    ggCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCg                                                                                              <  1:4101331/150‑1 (MQ=255)

                                                      cACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGGTAGAGGAATTTTTTTAACAACCGaa                                                                                            >  2:4093577/1‑150 (MQ=255)

                                                       actactGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGaaa                                                                                           <  2:1661277/150‑1 (MQ=255)

                                                        ctactGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAAc                                                                                          >  1:3406564/1‑150 (MQ=255)

                                                         tactGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAAcc                                                                                         >  1:3471960/1‑150 (MQ=255)

                                                               cACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCAtct                                                                                   >  1:2230516/1‑150 (MQ=255)

                                                               cACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCAtct                                                                                   <  2:3467515/150‑1 (MQ=255)

                                                                   aaaGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGAc                                                                               <  2:3169247/150‑1 (MQ=255)

                                                                     aGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACACCATCTCGACaa                                                                             <  1:3376/150‑1 (MQ=255)

                                                                       gTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAAtt                                                                           <  1:2067788/150‑1 (MQ=255)

                                                                          aaCGCGCGAGTCATCTCTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCt                                                                        <  1:1821178/150‑1 (MQ=255)

                                                                          aaCGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCt                                                                        >  2:3701808/1‑150 (MQ=255)

                                                                           aCGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTa                                                                       >  1:3624762/1‑150 (MQ=255)

                                                                              cgcgAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTTAGCAACCGAAACCCCATCTCGACAATTTCTACCg                                                                    >  2:4293941/1‑150 (MQ=255)

                                                                              cgcgAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCg                                                                    <  2:3266458/150‑1 (MQ=255)

                                                                                cgAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGa                                                                  >  1:3940291/1‑150 (MQ=255)

                                                                                cgAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGa                                                                  <  2:535255/150‑1 (MQ=255)

                                                                                 gAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGaa                                                                 <  2:1193289/150‑1 (MQ=255)

                                                                                   gTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAAtt                                                               <  2:1868593/150‑1 (MQ=255)

                                                                                    tcatcaCTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTc                                                              <  2:3768141/150‑1 (MQ=255)

                                                                                    tcatcaCTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTc                                                              >  2:2252664/1‑150 (MQ=255)

                                                                                     catcaCTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTcc                                                             >  1:941032/1‑150 (MQ=255)

                                                                                      atcaCTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCt                                                            <  2:3570139/150‑1 (MQ=255)

                                                                                       tcaCTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTa                                                           <  2:1752866/150‑1 (MQ=255)

                                                                                         aCTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATg                                                         <  2:2740774/150‑1 (MQ=255)

                                                                                          cTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGc                                                        <  2:4187816/150‑1 (MQ=255)

                                                                                               aTCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCa                                                   >  1:2612713/1‑150 (MQ=255)

                                                                                                tCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCAt                                                  >  1:2055365/1‑150 (MQ=255)

                                                                                                tCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCAt                                                  <  2:717341/150‑1 (MQ=255)

                                                                                                   cgATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATTCGAAGAGAAGGTCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCa                                               >  2:879250/1‑150 (MQ=255)

                                                                                                    gATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCAc                                              >  1:3792637/1‑150 (MQ=255)

                                                                                                       atCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTc                                           >  1:2378540/1‑150 (MQ=255)

                                                                                                         cAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCcg                                         <  2:1013534/150‑1 (MQ=255)

                                                                                                          aaTACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCcgc                                        <  2:1447009/150‑1 (MQ=255)

                                                                                                          aaTACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCcgc                                        <  1:3866663/150‑1 (MQ=255)

                                                                                                            tACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGa                                      >  1:3338086/1‑150 (MQ=255)

                                                                                                              cTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACg                                    <  2:398039/150‑1 (MQ=255)

                                                                                                               tGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACgg                                   >  2:1528153/1‑150 (MQ=255)

                                                                                                                gTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGt                                  >  1:1999290/1‑150 (MQ=255)

                                                                                                                gTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGt                                  <  2:3996622/150‑1 (MQ=255)

                                                                                                                gTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGt                                  >  1:3728885/1‑150 (MQ=255)

                                                                                                                  tAATCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTAt                                <  2:1358054/150‑1 (MQ=255)

                                                                                                                   aaTCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATg                               <  2:193299/150‑1 (MQ=255)

                                                                                                                   aaTCGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATg                               <  1:2817729/150‑1 (MQ=255)

                                                                                                                      cGAGAAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTg                            >  2:1698000/1‑150 (MQ=255)

                                                                                                                       gagaAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTgg                           <  1:1212260/150‑1 (MQ=255)

                                                                                                                         gaAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGAt                         >  1:202120/1‑150 (MQ=255)

                                                                                                                         gaAATGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGAt                         <  2:2184876/150‑1 (MQ=255)

                                                                                                                          aaaTGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGAtt                        <  1:1317043/150‑1 (MQ=255)

                                                                                                                           aaTGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGAttt                       <  2:2665278/150‑1 (MQ=255)

                                                                                                                             tGGTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGt                     <  1:2424345/150‑1 (MQ=255)

                                                                                                                              ggTAGACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTa                    >  1:3004021/1‑150 (MQ=255)

                                                                                                                                  gACCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGTACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAg                <  1:604213/150‑1 (MQ=255)

                                                                                                                                   aCCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGc               <  2:2790657/150‑1 (MQ=255)

                                                                                                                                   aCCTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGc               <  2:3091308/150‑1 (MQ=255)

                                                                                                                                     cTGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCt             >  1:3164902/1‑150 (MQ=255)

                                                                                                                                      tGAAGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAACACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATACTGGATTTGTATTAGCCtt            >  2:1298168/1‑150 (MQ=255)

                                                                                                                                        aaGTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTc          >  1:2951012/1‑150 (MQ=255)

                                                                                                                                          gTTTTTCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGa        <  2:6388/150‑1 (MQ=255)

                                                                                                                                           tttttCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGAc       >  2:3888828/1‑150 (MQ=255)

                                                                                                                                           tttttCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGAc       >  1:2899881/1‑150 (MQ=255)

                                                                                                                                               tCTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCg   <  1:308128/150‑1 (MQ=255)

                                                                                                                                                cTGTGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGt  >  1:1632800/1‑150 (MQ=255)

CTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAATGGTAGACCTGAAGTTTTTCTGCGGCGGAATAATCCGAAGAGAAGATCACCGGATAGAGGATTTTTTTCAGCACCCGAAACCCCATCTCGACAATTTCTACCGGAATTCCTATGCGGGCATGCACTTCCGCGACGGTATGCTGGATTTGTATTAGCCTTTCGACATCGT  >  AP012306/1273430‑1273723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`