BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	1,844,249	Δ1 bp	coding (124/567 nt)	spr →	predicted peptidase, outer membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	1,844,249	0	G	.	100.0%	1543.5 / NA	309	coding (124/567 nt)	spr	predicted peptidase, outer membrane lipoprotein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (163/146); total (163/146)

Only 100 of 314 total aligned reads displayed.

ACTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAA  >  AP012306/1844104‑1844393

aCTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTg                                                                                                                                             >  1:2165330/1‑150 (MQ=255)

 cTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTgg                                                                                                                                            <  2:3723808/150‑1 (MQ=255)

 cTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTgg                                                                                                                                            >  1:1327797/1‑150 (MQ=255)

 cTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTgg                                                                                                                                            <  1:3676293/150‑1 (MQ=255)

 cTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTgg                                                                                                                                            >  2:680432/1‑150 (MQ=255)

  ttCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTggg                                                                                                                                           >  2:2911200/1‑150 (MQ=255)

   tCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGt                                                                                                                                          <  2:1552929/150‑1 (MQ=255)

   tCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGt                                                                                                                                          <  1:2211946/150‑1 (MQ=255)

     aaGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAg                                                                                                                                        >  1:2414705/1‑150 (MQ=255)

      aGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGt                                                                                                                                       <  2:3588952/150‑1 (MQ=255)

        ggAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGGGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGa                                                                                                                                     >  2:1166483/1‑150 (MQ=255)

            aacaaacaACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAAca                                                                                                                                 >  1:430364/1‑150 (MQ=255)

            aacaaacaACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAAca                                                                                                                                 <  1:1643013/150‑1 (MQ=255)

                aacaacATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAAcatcat                                                                                                                             <  1:3343415/150‑1 (MQ=255)

                  caacaTGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACAtcatca                                                                                                                           >  2:1100133/1‑150 (MQ=255)

                    acaTGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACt                                                                                                                         >  2:3483234/1‑150 (MQ=255)

                    acaTGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACt                                                                                                                         <  2:2153424/150‑1 (MQ=255)

                     caTGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTg                                                                                                                        <  1:2518035/150‑1 (MQ=255)

                      aTGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGc                                                                                                                       >  2:651605/1‑150 (MQ=255)

                      aTGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGc                                                                                                                       <  1:1440311/150‑1 (MQ=255)

                         gTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAg                                                                                                                    >  2:2628566/1‑150 (MQ=255)

                         gTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAg                                                                                                                    >  2:1255985/1‑150 (MQ=255)

                           cAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCt                                                                                                                  >  1:3842029/1‑150 (MQ=255)

                            aaaTCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCtt                                                                                                                 >  2:3815426/1‑150 (MQ=255)

                                   aaCCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGa                                                                                                          >  1:1540399/1‑150 (MQ=255)

                                   aaCCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGa                                                                                                          >  1:1254644/1‑150 (MQ=255)

                                    aCCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGAt                                                                                                         <  1:3867866/150‑1 (MQ=255)

                                    aCCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGAt                                                                                                         <  1:3438980/150‑1 (MQ=255)

                                    aCCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGAt                                                                                                         <  1:1221832/150‑1 (MQ=255)

                                       gATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGaa                                                                                                      >  2:2427255/1‑150 (MQ=255)

                                       gATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGaa                                                                                                      >  2:3295046/1‑150 (MQ=255)

                                       gATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGaa                                                                                                      >  1:1028013/1‑150 (MQ=255)

                                       gATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGaa                                                                                                      <  2:888476/150‑1 (MQ=255)

                                          tttGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTCCATGTGGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTGGAGAAACAACATCACTGCAAGCTTCTCGGGATAAAttt                                                                                                   <  2:2746041/150‑1 (MQ=255)

                                            tGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGa                                                                                                 >  2:3161881/1‑150 (MQ=255)

                                              agaTATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGaaa                                                                                               <  2:254414/150‑1 (MQ=255)

                                                 tatatCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAAcc                                                                                            >  1:1411712/1‑150 (MQ=255)

                                                  atatCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCt                                                                                           <  1:2114443/150‑1 (MQ=255)

                                                  atatCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGCGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCCAGCTTCTCAGGCTGAATTTGAAAACCt                                                                                           >  2:1888875/1‑150 (MQ=255)

                                                   tatCTTGCGCGGGATCCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTg                                                                                          <  2:40856/150‑1 (MQ=255)

                                                    atCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTgg                                                                                         >  1:2308722/1‑150 (MQ=255)

                                                     tCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACTCCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGt                                                                                        <  2:3329205/150‑1 (MQ=255)

                                                      cTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGtt                                                                                       >  1:1667821/1‑150 (MQ=255)

                                                        tGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCg                                                                                     <  2:1844731/150‑1 (MQ=255)

                                                          cgcgGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTa                                                                                   <  1:457312/150‑1 (MQ=255)

                                                           gcgGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTaa                                                                                  >  2:1739789/1‑150 (MQ=255)

                                                              ggATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGt                                                                               <  2:2026990/150‑1 (MQ=255)

                                                                 ttCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGa                                                                            <  1:3741590/150‑1 (MQ=255)

                                                                  tCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGAc                                                                           <  1:700178/150‑1 (MQ=255)

                                                                  tCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGAc                                                                           >  2:3077105/1‑150 (MQ=255)

                                                                     cgcgATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTa                                                                        <  2:3042136/150‑1 (MQ=255)

                                                                       cgATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTaaa                                                                      <  1:3577330/150‑1 (MQ=255)

                                                                        gATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTaaaa                                                                     >  2:2443599/1‑150 (MQ=255)

                                                                            gCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATcgc                                                                 <  2:483662/150‑1 (MQ=255)

                                                                             cAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATcgcg                                                                <  1:3024413/150‑1 (MQ=255)

                                                                             cAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATcgcg                                                                >  1:3080016/1‑150 (MQ=255)

                                                                               gTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGaa                                                              >  1:2051777/1‑150 (MQ=255)

                                                                                tAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAAt                                                             >  1:2806422/1‑150 (MQ=255)

                                                                                 aGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAAtt                                                            >  2:1734164/1‑150 (MQ=255)

                                                                                  gCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTa                                                           <  1:3982947/150‑1 (MQ=255)

                                                                                  gCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTa                                                           >  2:240781/1‑150 (MQ=255)

                                                                                   cGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTAt                                                          <  1:3181778/150‑1 (MQ=255)

                                                                                    ggTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATg                                                         <  1:2545038/150‑1 (MQ=255)

                                                                                    ggTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATg                                                         <  2:1408611/150‑1 (MQ=255)

                                                                                      ttCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGa                                                       <  2:843889/150‑1 (MQ=255)

                                                                                      ttCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGa                                                       >  2:393013/1‑150 (MQ=255)

                                                                                       tCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGAt                                                      <  1:1111947/150‑1 (MQ=255)

                                                                                       tCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGAt                                                      >  2:507726/1‑150 (MQ=255)

                                                                                          gCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAg                                                   >  1:872301/1‑150 (MQ=255)

                                                                                              tCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATg                                               >  1:2373842/1‑150 (MQ=255)

                                                                                                 gCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTg                                            <  1:1603477/150‑1 (MQ=255)

                                                                                                 gCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTg                                            <  2:3688752/150‑1 (MQ=255)

                                                                                                 gCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGAGAATTATGGATCAGTATGCTg                                            <  1:350424/150‑1 (MQ=255)

                                                                                                   aTGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGAc                                          >  1:1766979/1‑150 (MQ=255)

                                                                                                   aTGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGAc                                          >  1:292268/1‑150 (MQ=255)

                                                                                                     gTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTg                                        <  2:1382084/150‑1 (MQ=255)

                                                                                                     gTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTg                                        <  1:2596383/150‑1 (MQ=255)

                                                                                                      tAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTgg                                       <  2:1471857/150‑1 (MQ=255)

                                                                                                       aGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGa                                      >  1:3792916/1‑150 (MQ=255)

                                                                                                         tGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGaaa                                    >  2:1603644/1‑150 (MQ=255)

                                                                                                          gCAAATAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAg                                   >  1:538132/1‑150 (MQ=255)

                                                                                                            aaaTAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGc                                 <  1:1473637/150‑1 (MQ=255)

                                                                                                            aaaTAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGc                                 >  1:3060252/1‑150 (MQ=255)

                                                                                                             aaTAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCg                                <  2:575326/150‑1 (MQ=255)

                                                                                                              aTAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGt                               >  2:3765686/1‑150 (MQ=255)

                                                                                                               tAACACCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTa                              <  1:926430/150‑1 (MQ=255)

                                                                                                                 acacCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACg                            >  2:1866252/1‑150 (MQ=255)

                                                                                                                 acacCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACg                            >  1:993470/1‑150 (MQ=255)

                                                                                                                  cacCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGt                           >  1:1712849/1‑150 (MQ=255)

                                                                                                                   acCGCAAAGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGtt                          <  2:3392369/150‑1 (MQ=255)

                                                                                                                          aGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCt                   >  1:3234887/1‑150 (MQ=255)

                                                                                                                          aGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCt                   >  1:814114/1‑150 (MQ=255)

                                                                                                                          aGAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCt                   >  2:1183892/1‑150 (MQ=255)

                                                                                                                           gAATATGCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTg                  >  1:60977/1‑150 (MQ=255)

                                                                                                                                 gCATCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGggcggc            >  2:2114021/1‑150 (MQ=255)

                                                                                                                                   aTCCTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGgcag          <  2:1974068/150‑1 (MQ=255)

                                                                                                                                     ccTGAGACACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGgcagca        <  1:2454163/150‑1 (MQ=255)

                                                                                                                                          gaCACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTaaa   <  1:2719949/150‑1 (MQ=255)

                                                                                                                                          gaCACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTaaa   <  1:1781746/150‑1 (MQ=255)

                                                                                                                                          gaCACGT‑CAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTaaa   <  2:1321908/150‑1 (MQ=255)

ACTTCAAGGGAAAACAAACAACATGGTCAAATCTCAACCGATTTTGAGATATATCTTGCGCGGGATTCCCGCGATTGCAGTAGCGGTTCTGCTTTCTGCATGTAGTGCAAATAACACCGCAAAGAATATGCATCCTGAGACACGTGCAGTGGGTAGTGAAACATCATCACTGCAAGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAA  >  AP012306/1844104‑1844393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`