Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306935,0540GT30.4% 217.8 / 52.0 92intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
Reads supporting (aligned to +/- strand):  ref base G (17/47);  new base T (28/0);  total (45/47)
Fisher's exact test for biased strand distribution p-value = 3.43e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 157 total aligned reads displayed.

CAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACA  >  AP012306/934907‑935203
                                                                                                                                                    |                                                                                                                                                           
cAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTtttttt                                                                                                                                                           >  1:3836147/1‑150 (MQ=255)
cAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTtttgtt                                                                                                                                                           >  1:2399374/1‑150 (MQ=255)
  gTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGttttttttt                                                                                                                                                         >  1:238572/1‑149 (MQ=255)
  gTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTtttgtttg                                                                                                                                                         <  1:66050/150‑1 (MQ=255)
  gTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGtttttttt                                                                                                                                                         >  2:1222916/1‑149 (MQ=255)
  gTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGAtttttttt                                                                                                                                                         >  2:3156455/1‑149 (MQ=255)
  gTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAACCTAAGGGACTTAGCCTGCGGC‑GGttttttttt                                                                                                                                                         >  2:3695153/1‑149 (MQ=255)
   tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGttttttttg                                                                                                                                                        >  2:3250668/1‑148 (MQ=255)
      tGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTtttgttttggtt                                                                                                                                                     >  2:1131500/1‑145 (MQ=255)
      tGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCtt                                                                                                                                                     <  1:1021617/150‑1 (MQ=255)
        ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTcc                                                                                                                                                   >  2:3781167/1‑149 (MQ=255)
        ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCttta                                                                                                                                                   >  1:3063111/1‑148 (MQ=255)
        ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGCttta                                                                                                                                                   >  2:3393600/1‑148 (MQ=255)
           ttGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTctgca                                                                                                                                                >  2:573184/1‑150 (MQ=255)
           ttGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTcagca                                                                                                                                                <  2:1044633/150‑1 (MQ=255)
           ttGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTcagca                                                                                                                                                <  1:2853083/150‑1 (MQ=255)
            tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTcagcag                                                                                                                                               >  1:94479/1‑150 (MQ=255)
            tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTTGCTTccgcag                                                                                                                                               >  1:3212302/1‑150 (MQ=255)
            tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTcagcag                                                                                                                                               <  2:6728/150‑1 (MQ=255)
             gTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCagcagc                                                                                                                                              <  1:2863222/150‑1 (MQ=255)
               aGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTGGGTTTAGCAGC‑gg                                                                                                                                           >  2:2839038/1‑150 (MQ=255)
                gtgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTCGGTTTAGCCGC‑cgg                                                                                                                                          >  1:511196/1‑150 (MQ=255)
                  gtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGGTTTTGTTTGG‑TTCAGGAGG‑GGGtt                                                                                                                                        >  2:88534/1‑150 (MQ=255)
                   tttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTg                                                                                                                                       <  1:654728/150‑1 (MQ=255)
                     tAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTgcg                                                                                                                                     <  1:307976/150‑1 (MQ=255)
                      aGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTgcg‑c                                                                                                                                   <  1:3158819/150‑1 (MQ=255)
                       gTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTGGCTTCAGCAAC‑ggggttgggt                                                                                                                                   >  1:3643294/1‑143 (MQ=255)
                       gTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑t                                                                                                                                 <  1:1760508/150‑1 (MQ=255)
                       gTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTGTTTTGGTTTCGCAGC‑gggggttgct                                                                                                                                   >  2:1935436/1‑143 (MQ=255)
                         ttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCCGCAGC‑GGGGTGCG‑C‑Tcc                                                                                                                               >  1:1198803/1‑150 (MQ=255)
                         ttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑Tcc                                                                                                                               <  1:1405432/150‑1 (MQ=255)
                            cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCtt                                                                                                                            <  1:3531968/150‑1 (MQ=255)
                            cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCtt                                                                                                                            <  2:183152/150‑1 (MQ=255)
                             tGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTTAGCAGC‑GGGGTGCG‑C‑Tcccctt                                                                                                                           >  1:2120869/1‑147 (MQ=255)
                              gggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTTTCTTTAACAGC‑GGGGTGCG‑C‑CCCCttta                                                                                                                          >  1:2783561/1‑148 (MQ=255)
                               ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTTCGCAGC‑GGGGTGCG‑C‑cccccttat                                                                                                                         >  2:2528662/1‑145 (MQ=255)
                                gTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTTGCTTCCCCAGC‑GGGTTGCG‑C‑TTCCTTTAtg                                                                                                                        >  1:1686821/1‑150 (MQ=255)
                                 tAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGCTTTCCCAAC‑GGGGTGCG‑C‑Tccccttaagt                                                                                                                       >  2:319009/1‑143 (MQ=255)
                                  aaaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCAGCAGC‑GGGGTTCG‑C‑TCCCCTTAtgtg                                                                                                                      >  1:687863/1‑150 (MQ=255)
                                  aaaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAAtgtg                                                                                                                      <  2:2280796/150‑1 (MQ=255)
                                   aaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGc                                                                                                                     <  2:2059393/150‑1 (MQ=255)
                                    aGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCTCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTTGGTTCACCCGC‑GGGGTGGC‑C‑TCCCCTTATTTGcc                                                                                                                    >  1:310390/1‑150 (MQ=255)
                                     gcgcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCt                                                                                                                   <  1:2052866/150‑1 (MQ=255)
                                      cgcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTc                                                                                                                  <  1:848003/150‑1 (MQ=255)
                                       gcCGTCAGCGGCGATGCAAAAACGCTTTGCCTAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTGTTTGGGGTCAGGAGC‑GGGGTGGG‑C‑TCCCCTAAagtgggtcg                                                                                                                 >  1:3107475/1‑145 (MQ=255)
                                       gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCTGCAAC‑GGGGTGCG‑C‑TCCCCTAATGTGCCTcc                                                                                                                 >  1:3804237/1‑149 (MQ=255)
                                       gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGCTTCAGCAGC‑CGGGTGCG‑C‑CCCCCTAAATTGCCTcc                                                                                                                 >  1:1113524/1‑149 (MQ=255)
                                        ccGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTTGCTTCAGCAGC‑GGGGTGCG‑C‑TCCCCTACTTTGcccccc                                                                                                                >  1:1803620/1‑146 (MQ=255)
                                        ccGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGATTTTTTTTGGTTCAGCACC‑CGGTTGGG‑C‑TCCCTTTATATGGCTctc                                                                                                                >  1:1775212/1‑148 (MQ=255)
                                         cGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTTCGCAGC‑GGGGTGCG‑C‑CCCCTTTATGTGCCTcccc                                                                                                               >  2:1649656/1‑150 (MQ=255)
                                         cGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCCGCAGC‑GGGTTGCG‑C‑TCCCCTTATTTGCCCCGcc                                                                                                               >  1:1021676/1‑150 (MQ=255)
                                         cGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTTGGTTCCGCAGC‑GGGGTGCG‑C‑TCCCTTAATGTGCCTCGcc                                                                                                               >  1:279189/1‑150 (MQ=255)
                                          gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTTGGTTCAGCAGC‑GGGGTGCG‑C‑TCCCCTAAAGTGCCCCGccc                                                                                                              >  1:3532088/1‑149 (MQ=255)
                                          gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGGTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTGTTTTGGTTTAGGAGC‑GGGGTGGG‑G‑TCCCCTTAAGTGGCTCGGCa                                                                                                              >  1:2691257/1‑150 (MQ=255)
                                            cAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCAGCAGC‑GGGGTGCG‑C‑TCCCCTAATTTGCCTCCCCcta                                                                                                            >  1:1142316/1‑150 (MQ=255)
                                             aGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGCTTTTTTTTGCTTTAGAGGA‑GGGGTGCG‑C‑CCCCCTCAAGTGCgccgccccat                                                                                                           >  2:2594071/1‑146 (MQ=255)
                                              gcggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCAGCAGC‑GGGGTGCG‑C‑TCCCCTTATGTGGCTCGCCCta‑t‑a                                                                                                        >  1:1709692/1‑150 (MQ=255)
                                               cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTTGCTTCAGCAGC‑GGGGTGCG‑C‑CCCCCTAATGTGGCTCGCCCTA‑T‑aa                                                                                                       >  1:1451495/1‑150 (MQ=255)
                                                 gcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCAGCCGC‑GGGGTGCG‑C‑GCCCCTTATGTGGCCCGCCCTT‑T‑AAAt                                                                                                     >  2:379830/1‑150 (MQ=255)
                                                    aTGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTGGCTTCAGCAGC‑GGGGTGCG‑C‑CCCCCTTATGTGCCTCGCCCTA‑T‑aaaatta                                                                                                  >  1:252348/1‑146 (MQ=255)
                                                         aaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGGTGCG‑C‑TCCCTTAATGTGCCTCGCCCTA‑T‑AAATTTTATGGt                                                                                             >  1:3132807/1‑150 (MQ=255)
                                                           aaCGCTTTGCGGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCCGCAAC‑CGGGTGCG‑C‑TCCCCTTATTTTCCCCCCCCTA‑T‑AAATTTAATTGTGc                                                                                           >  2:195829/1‑150 (MQ=255)
                                                           aaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTTTGCAGCGGGGTTCGG‑G‑TCCCTT‑ATTTGGCCCCCCATA‑T‑TAATTGTATtgtgg                                                                                           >  2:3878402/1‑149 (MQ=255)
                                                            aCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTTTGCAGC‑GGGGTGCC‑C‑TCCCTTAATATGGCTCCCCCAA‑T‑TAATTTAATGGGGCa                                                                                          >  1:3738788/1‑150 (MQ=255)
                                                              gCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAgg                                                                                        <  2:2985977/150‑1 (MQ=255)
                                                                tttGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCAGCAGC‑GGGGTGGG‑C‑TCCCCTTATTTGCCCCGCCCTA‑T‑AAATTTAATGGTGCAgggg                                                                                      >  2:1114326/1‑149 (MQ=255)
                                                                  tGCCGGGGCCGATGCGCACTATCATTCGGCTCCGGCGGCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAgc                                                                                    <  2:1611229/150‑1 (MQ=255)
                                                                   gCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTCCGCCGC‑GGGGGGCG‑C‑GCCCCCAATGTGCCTCGCCCTA‑A‑AAATTGAAAGGGGGAggggggg                                                                                   >  2:3490984/1‑146 (MQ=255)
                                                                   gCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGGGTCAGCAGC‑GGGGGGCG‑G‑CCCCCTTATGTGGCCCCCCCTA‑T‑TAAATTAAAGGTGGAggggggg                                                                                   >  2:2027069/1‑146 (MQ=255)
                                                                    ccGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTTCGCCGC‑GGGGTTCGCC‑CCCCTTATTGGGCC‑CCCCCTA‑TAAAATTGAA‑GGGGGAGGGAgcgc                                                                                  >  2:1799530/1‑150 (MQ=255)
                                                                    ccGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTCAGGCGC‑GGGGTGCG‑C‑TTCCCTTATTTGCCCCCCCCTA‑T‑AAATTTAATTGTGCAGGGGgcgc                                                                                  >  2:655454/1‑150 (MQ=255)
                                                                        ggCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAg                                                                             <  1:2845460/150‑1 (MQ=255)
                                                                                       tCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATcgc                                                              <  1:2494922/150‑1 (MQ=255)
                                                                                                  cgccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCct                                                   <  1:2206301/150‑1 (MQ=255)
                                                                                                   gccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTTGGTTAGCGGC‑GGGTTGGG‑C‑TCCCTTTATGTGCCCTCCCCTA‑TAAAATTTAAT‑GTGGAGGGGGCGCG‑CAGGGGGCGGGCCATAGCCGCCGccccccg                                                  >  2:2092903/1‑148 (MQ=255)
                                                                                                   gccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTTCGCAGC‑GGGGTGGG‑C‑CCCCCTTATGTGGCCCGCCCTT‑T‑AAATTTTATTGGGGGGGGGGGGCGCCGGGGGGGGGCCAAccccccccccccccc                                                   >  2:3139984/1‑149 (MQ=255)
                                                                                                       tCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGGTTCAGCAGC‑GGGGTGGG‑C‑TCCCTTTATTTGGCTCGCCCTA‑T‑AAATTTAATGGTGGAGGGGGGGCG‑CCGGGGGGGGCCAAACGCCGCCCCCCCCCCCTGt                                              >  2:3682590/1‑150 (MQ=255)
                                                                                                         aCGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTTTTTTTGCAGC‑GGGGTGCG‑C‑TCCCCTTATGTGCCTCGCCCTA‑T‑AAATTTAATGGTGGAGGGGGGGCG‑GCGGGGGGGGCCAATCGCCGCCCCCCCCCCCTGTcc                                            >  2:2721062/1‑150 (MQ=255)
                                                                                                           ggTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGGTTTTTTTTGGTTCTGCAGC‑GGGGTGCG‑CGCCCCTTATTGGGCC‑CGCCCTATT‑AAATTAAAGGGGGCAGGGGGGGGC‑CGGGGGGGGGCCAA‑CCCCCCCCCCCCCCCCTGTcccc                                          >  2:3938346/1‑149 (MQ=255)
                                                                                                              tGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGTGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGcc                                       <  2:2439676/150‑1 (MQ=255)
                                                                                                                  ggAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCg                                   <  2:3540231/150‑1 (MQ=255)
                                                                                                                      aGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGa                               <  1:1950317/150‑1 (MQ=255)
                                                                                                                       gCTAAGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCTGCAGC‑GGGGTGCG‑C‑TCCCCTTATGTGGCTCGCCCTA‑T‑AAATTTTATGGTGGCGGGGGCGCG‑CCGGGGGGGGCCCATCGCCCCCCCCCCCCCCTCTCCCGGCCCTCgggggg                              >  2:2023490/1‑148 (MQ=255)
                                                                                                                          aaGGGACTTAGCCTGCGGC‑GGTTTTTTTTTGCTTCCGCAGC‑GGGGTGGG‑C‑CCCCCTTATGTGCCTCGCCCTA‑T‑AAATTTTATGGTGCAGGGGGGGCG‑CAGGGGGGGGCCCATCGCCGCCGCCCCCCGCTGTCCCCGGCTTCgggggaagc                           >  2:285735/1‑145 (MQ=255)
                                                                                                                             ggACTTAGCCTGCGGC‑GGTTTTTTTTGGGTTCAGCAAC‑GGGGTTCG‑C‑CCCCCTAATGTGCCTCGCCCTA‑T‑AAAATTAATGGTGGAGGGGGCGCG‑CGGGGGGGGGGCCATCGCCCCCCCCCCCCGCTGTCCCCGCCCTCCGGGGACGCTTc                        >  2:1310560/1‑150 (MQ=255)
                                                                                                                              gACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCAGGGAACGCTTCa                       <  2:3992361/150‑1 (MQ=255)
                                                                                                                                     ccTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGAttt                <  2:116939/150‑1 (MQ=255)
                                                                                                                                      cTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGAtttt               <  2:1607530/150‑1 (MQ=255)
                                                                                                                                          ggc‑ggTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACg           <  1:3650370/150‑1 (MQ=255)
                                                                                                                                              ggTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGcca        >  2:1486033/1‑150 (MQ=255)
                                                                                                                                               gTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccac       <  2:510840/150‑1 (MQ=255)
                                                                                                                                                ttttGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacc      >  2:1328399/1‑150 (MQ=255)
                                                                                                                                                ttttGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacc      >  1:1099100/1‑150 (MQ=255)
                                                                                                                                                 tttgtttgGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacca     >  2:1068361/1‑150 (MQ=255)
                                                                                                                                                  ttgtttgGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCaa    <  2:2435177/150‑1 (MQ=255)
                                                                                                                                                    gtttgGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAaca  <  1:2643789/150‑1 (MQ=255)
                                                                                                                                                    gtttgGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAaca  >  1:3924936/1‑150 (MQ=255)
                                                                                                                                                    gtttgGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAaca  >  1:1632678/1‑150 (MQ=255)
                                                                                                                                                    gtttgGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAaca  <  2:3005345/150‑1 (MQ=255)
                                                                                                                                                    gtttgGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAaca  >  1:2757943/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                           
CAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTGTTTGGCTTCAGCAGC‑GGGTTGCG‑C‑TCCCTTAATGTGCCTCGCCATA‑T‑AAATTGAATGGTGCAGGGAGCGCG‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACA  >  AP012306/934907‑935203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑