Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,226,5570AG24.9% 397.1 / 75.5 177G55G (GGA→GGG) ydcUpredicted spermidine/putrescine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (18/115);  new base G (44/0);  total (62/115)
Fisher's exact test for biased strand distribution p-value = 2.09e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 199 total aligned reads displayed.

TTGCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGC  >  AP012306/1226408‑1226706
                                                                                                                                                     |                                                                                                                                                     
 tGCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAt                                                                                                                                                      <  1:3629165/150‑1 (MQ=255)
  gCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAtt                                                                                                                                                     <  1:1253951/150‑1 (MQ=255)
   cAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGttt                                                                                                                                                    >  2:3046342/1‑150 (MQ=255)
   cAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGttt                                                                                                                                                    >  2:2975341/1‑150 (MQ=255)
    aaTCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAtttt                                                                                                                                                   <  1:166339/150‑1 (MQ=255)
     aTCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTta                                                                                                                                                  <  2:3463858/150‑1 (MQ=255)
       cACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTtata                                                                                                                                                <  1:3486228/150‑1 (MQ=255)
        aCCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATAc                                                                                                                                               <  1:3851881/150‑1 (MQ=255)
        aCCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATAc                                                                                                                                               <  2:161362/150‑1 (MQ=255)
          cTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACtt                                                                                                                                             <  2:3125970/150‑1 (MQ=255)
           ttCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACttt                                                                                                                                            <  1:413234/150‑1 (MQ=255)
             cACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTg                                                                                                                                          <  2:423804/150‑1 (MQ=255)
               cGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACttttttc                                                                                                                                        >  1:986386/1‑147 (MQ=255)
                 tCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTGAAGa                                                                                                                                      >  1:3419105/1‑150 (MQ=255)
                  ccAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGAt                                                                                                                                     <  2:1210295/150‑1 (MQ=255)
                   cAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGAtt                                                                                                                                    <  1:89228/150‑1 (MQ=255)
                    aGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGAttt                                                                                                                                   <  1:2304346/150‑1 (MQ=255)
                        cTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTTACGATTTCAcc                                                                                                                               >  1:1366170/1‑150 (MQ=255)
                        cTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCAcc                                                                                                                               <  1:3227127/150‑1 (MQ=255)
                         tGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCa                                                                                                                              <  1:2069702/150‑1 (MQ=255)
                           ggTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATg                                                                                                                            <  1:1773759/150‑1 (MQ=255)
                            gTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGt                                                                                                                           <  1:2709966/150‑1 (MQ=255)
                              aaGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGATTTTACCCTGTCg                                                                                                                         >  2:157763/1‑150 (MQ=255)
                               aGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTTCTTTTTGCGATTTTCCCATGTTgg                                                                                                                        >  2:2105819/1‑150 (MQ=255)
                                 gtgtCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAGTTTATACTTTTTACGAATTCACCATGTCggga                                                                                                                      >  1:487273/1‑148 (MQ=255)
                                  tgtCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTaa                                                                                                                     <  2:212563/150‑1 (MQ=255)
                                     ccGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGGTTTCTCCCTGTCGGGAAcac                                                                                                                  >  1:796526/1‑148 (MQ=255)
                                         tttCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTGCGGTTTTCCCCTGTTGGTAACGCCggg                                                                                                              >  2:2639147/1‑149 (MQ=255)
                                          ttcttcTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTTCCATGTTGGGAAAGCCggga                                                                                                             >  2:4124706/1‑148 (MQ=255)
                                          ttcttcTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTACCATGTCGGGAACGCCggga                                                                                                             >  1:1102718/1‑148 (MQ=255)
                                              tctGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGa                                                                                                         <  1:4047876/150‑1 (MQ=255)
                                               ctGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCATTTTACGATTTCAACATGTTCGTAACGCCGGAACTGAc                                                                                                        >  2:815119/1‑150 (MQ=255)
                                                tGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACg                                                                                                       <  1:2983733/150‑1 (MQ=255)
                                                   cATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTGCGATTTTTCCCTGTCGGTAACGCCCGAACTGACGccg                                                                                                    >  2:527003/1‑148 (MQ=255)
                                                    aTAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTgg                                                                                                   <  1:304567/150‑1 (MQ=255)
                                                      aaTCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCg                                                                                                 <  2:2947335/150‑1 (MQ=255)
                                                       aTCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTTCGATTTTACCATGTCGGTAACGCCGGGACTGACGCCGGCgg                                                                                                >  2:1461494/1‑149 (MQ=255)
                                                         ccGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAat                                                                                              <  1:1064997/150‑1 (MQ=255)
                                                          cGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAata                                                                                             <  1:1924660/150‑1 (MQ=255)
                                                          cGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAata                                                                                             <  1:53870/150‑1 (MQ=255)
                                                            gggctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTAATTTTTACGATTTTACCCTGTCGGTAACGCCGGAACTGACGCTGGCGGatatt                                                                                           >  1:156463/1‑149 (MQ=255)
                                                            gggctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATc                                                                                           <  1:1458511/150‑1 (MQ=255)
                                                                tggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTg                                                                                       <  1:1007611/150‑1 (MQ=255)
                                                                 ggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTgc                                                                                      <  1:955490/150‑1 (MQ=255)
                                                                 ggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTgc                                                                                      <  2:730323/150‑1 (MQ=255)
                                                                   ggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGATTTCCCCCTTTTGGTAAACCCGGAACTTACGCCGGCGGAAATTCGTgcgc                                                                                    >  2:4087578/1‑150 (MQ=255)
                                                                    gctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCt                                                                                   <  1:2207660/150‑1 (MQ=255)
                                                                     ctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCCTTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTg                                                                                  <  2:3266918/150‑1 (MQ=255)
                                                                     ctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTg                                                                                  <  1:766043/150‑1 (MQ=255)
                                                                       gTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTACCATGTCGGTAACGCCGGAACTGACGCTGGCGGATATCCGTGCGCTGtt                                                                                >  2:858218/1‑150 (MQ=255)
                                                                       gTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCAGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGtt                                                                                <  1:1398921/150‑1 (MQ=255)
                                                                         ttttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTa                                                                              <  1:673972/150‑1 (MQ=255)
                                                                            tACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTTGCGAGTTCACCATGTTGGTAAAGCCGGGACTGACCCCGGCGAATATCCGCGGGCTGTTTTAtt                                                                           >  2:1861326/1‑149 (MQ=255)
                                                                            tACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATc                                                                           <  2:1134242/150‑1 (MQ=255)
                                                                             aCTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTGCGATTTTACCATGTCGGGAAAGCCGGAACTGACGCTGGCGGAAATTCGTGCGCCGTTTTAttc                                                                          >  2:2334258/1‑148 (MQ=255)
                                                                              ctgctgCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCg                                                                         <  1:3152832/150‑1 (MQ=255)
                                                                               tgctgcTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTACCCTGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCCTGCGCTGTTTTATCCgg                                                                        >  2:2668337/1‑150 (MQ=255)
                                                                                   gctTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGaa                                                                    <  1:2429235/150‑1 (MQ=255)
                                                                                     ttGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTGCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAAtt                                                                  <  2:1625010/150‑1 (MQ=255)
                                                                                      tGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTa                                                                 <  2:2214133/150‑1 (MQ=255)
                                                                                              tAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGAcatca                                                         <  1:2578815/150‑1 (MQ=255)
                                                                                                aTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCAtt                                                       <  1:2918992/150‑1 (MQ=255)
                                                                                                aTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCAtt                                                       <  2:2914365/150‑1 (MQ=255)
                                                                                                 tgtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATtc                                                      <  2:148388/150‑1 (MQ=255)
                                                                                                   tgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATtctc                                                    <  2:3928985/150‑1 (MQ=255)
                                                                                                    ggTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTcc                                                   <  1:2530031/150‑1 (MQ=255)
                                                                                                    ggTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTcc                                                   <  1:13190/150‑1 (MQ=255)
                                                                                                     gTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTACTTTTTGCGATTTTACCATTTCGGGAACGCCGGGACTGACGCTGGGGAATATCCGTGCGCTGTTTTATTCGGCGGAATACGACATCATTTTCCg                                                  >  2:2185299/1‑150 (MQ=255)
                                                                                                     gTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCg                                                  <  1:2843510/150‑1 (MQ=255)
                                                                                                      tttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTAATTTTGACGATTTTACCATGTCGGTAACGCCGGGACTGAAGCTGGGGAATATCCGTGCGCTGTTTTATCCCGCGGAATACGACATCATTTTcccc                                                 >  2:971299/1‑148 (MQ=255)
                                                                                                       ttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCa                                                >  2:308602/1‑150 (MQ=255)
                                                                                                           cATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTATTTTTTCGATTTTTCCATGTCGGGAAAGCCGGGACTGACGCTGGCGGATATACGGGCGCTGTTTTATCCGGCGGATTACGACATCATTTTCCGCCCGCt                                            >  2:1785791/1‑150 (MQ=255)
                                                                                                           cATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCt                                            <  1:329350/150‑1 (MQ=255)
                                                                                                           cATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCt                                            <  1:2804494/150‑1 (MQ=255)
                                                                                                              tGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTGCGGTTTTACCCTGTCGGGAACGCCCGGAATGGCGCTGGGGAATATCCCTGCGCTGTTTTATTCGGCGAAATTCGACATCATTTTCCGCACGCTGAc                                         >  2:1271881/1‑150 (MQ=255)
                                                                                                                  tATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGATTTTCCCATGTCGGTAACGCCGGGACTGACGCTGGCGAATATCCGTGCGCTGTTTTATCCGGCGAATTTCGACCTCATTCTCCCCCCGCTGGCTATg                                     >  2:601825/1‑150 (MQ=255)
                                                                                                                   aTTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTTCGGTTTTACCCTGTCGGTAACGCCGGGAATGACGCTGGCGGATATCCGTGGGCTGTTTTATTCGGCGAATTACGACATCATTTTCCGCCCGCTGACTATgg                                    >  2:2279084/1‑150 (MQ=255)
                                                                                                                   aTTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATgg                                    <  1:3117304/150‑1 (MQ=255)
                                                                                                                   aTTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATgg                                    <  1:3093435/150‑1 (MQ=255)
                                                                                                                        ggCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATTCGTGCGCTGTTTTATTCCGCGAATTACGACATCCTTCTCCGCACGCTGACTATTGCtgtg                               >  2:1984555/1‑150 (MQ=255)
                                                                                                                           tCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTGACGATTTTCCCATGTCGGTAACGCCCGGAATGACGCTGGCGAATATCCGGGCGCTGTTTTATCCGGCGAATTTAGACATCATTCTCCGCCCGCTGACTATGGGTggggga                            >  2:1778873/1‑148 (MQ=255)
                                                                                                                           tCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCa                            <  1:3768080/150‑1 (MQ=255)
                                                                                                                             gctgctGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGt                          <  1:3137342/150‑1 (MQ=255)
                                                                                                                              ctgctgACACTGTTATGGCAGGGATTTTTTACTTTTTACGATTTTACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCCCCCGCTGACTATTGCTGTGGCAGTc                         >  2:3442/1‑150 (MQ=255)
                                                                                                                              ctgctgACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTc                         <  2:192333/150‑1 (MQ=255)
                                                                                                                                  tgACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTACCATGTCGGGAACGCCGGGACTGACGCTGGCGAATATCCGTGCGCTGTTTTATCCGGCGAATTACGACATCATTCTCCGCACGCTGGCTATGGCTGTGGGGGTCCCTa                     >  2:2810262/1‑150 (MQ=255)
                                                                                                                                   gACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTag                    <  2:2470976/150‑2 (MQ=255)
                                                                                                                                     cacTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCg                  <  1:1020521/150‑1 (MQ=255)
                                                                                                                                     cacTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCg                  <  2:2541295/150‑1 (MQ=255)
                                                                                                                                       cTGTTATGGCAGGGGTTTTTTACTTTTGACGATTTCACCATGTCGGTAACGCCGGGACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGcc                >  2:3716882/1‑150 (MQ=255)
                                                                                                                                        tGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCa               <  2:3219176/150‑1 (MQ=255)
                                                                                                                                         gTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAg              <  2:2490082/150‑1 (MQ=255)
                                                                                                                                           tATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGCCACCATCGCCAgcg            >  2:638984/1‑150 (MQ=255)
                                                                                                                                              ggCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCa         >  1:3009035/1‑150 (MQ=255)
                                                                                                                                                cAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCAtt       <  1:849119/150‑1 (MQ=255)
                                                                                                                                                 aGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTc      >  2:476238/1‑150 (MQ=255)
                                                                                                                                                 aGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTc      >  1:1160517/1‑150 (MQ=255)
                                                                                                                                                   ggATTTTATACTTTTAACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTAAGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTg    <  2:2881849/150‑1 (MQ=255)
                                                                                                                                                    gATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTgg   <  1:2968262/150‑1 (MQ=255)
                                                                                                                                                    gATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTgg   >  1:2263431/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGC  >  AP012306/1226408‑1226706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑