| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP012306 | 406,677 | 0 | A | G | 21.7% | 488.6 / 15.6 | 189 | K294E (AAA→GAA) | hemH | ferrochelatase |
| Reads supporting (aligned to +/- strand): ref base A (36/112); new base G (41/0); total (77/112) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.96e-20 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 228 total aligned reads displayed.
GCCTTATACCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAA > AP012306/406529‑406826 | ccTTATACCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGgaa > 1:2471228/1‑150 (MQ=255) ccTTATACCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGgaa > 1:51643/1‑150 (MQ=255) ccTTATACCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGgaa > 1:3273151/1‑150 (MQ=255) cTTATACCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGgaaa > 1:1966088/1‑150 (MQ=255) cTTATACCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGaaaa < 2:1651993/150‑1 (MQ=255) ttATACCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGaaaaa < 1:2077201/150‑1 (MQ=255) tataCCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGgaaaaa > 2:409793/1‑150 (MQ=255) ataCCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAat < 2:2268532/150‑1 (MQ=255) aCCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAaaat > 1:4418732/1‑150 (MQ=255) gacgaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGaa > 1:884344/1‑150 (MQ=255) acgaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGgaaaaaaaataat > 2:881318/1‑143 (MQ=255) acgaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAat < 2:3137432/150‑1 (MQ=255) cgaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGAata > 1:2842758/1‑150 (MQ=255) cgaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAAAATGaaaa > 1:1158232/1‑148 (MQ=255) cgaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAAAATGAata > 1:3573523/1‑150 (MQ=255) gaAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATTAatat > 1:4153643/1‑150 (MQ=255) aaCGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAAATAatatat > 2:532920/1‑150 (MQ=255) aaCGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAatatat < 1:14780/150‑1 (MQ=255) aaCGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAAAATGAaaatat > 2:2543420/1‑150 (MQ=255) aCGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATAtt < 1:1106603/150‑1 (MQ=255) cGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTc < 1:4123859/150‑1 (MQ=255) cGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTc < 1:3045114/150‑1 (MQ=255) cGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTc < 1:2304095/150‑1 (MQ=255) gCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTcc < 1:2731076/150‑1 (MQ=255) cTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCg < 1:1549369/150‑1 (MQ=255) aaaaTGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATGAATATATTTCGgcg > 2:3054018/1‑150 (MQ=255) aaTGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑Ct < 2:4277922/150‑1 (MQ=255) gCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATGAATATATTCCGGCG‑CTT‑aa > 1:597515/1‑150 (MQ=255) ggAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGcc < 1:1708994/150‑1 (MQ=255) ggAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGcc < 2:3178765/150‑1 (MQ=255) agaAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCAc < 2:2642808/150‑1 (MQ=255) gcaaaaGGCGTAGGTCATATTCAGGTGATGTGCCCGCGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACg < 2:3583562/148‑1 (MQ=255) gaAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGTAAAAAAATAAATTTATTTCGGCGCCTT‑AAT‑CCACg > 1:462154/1‑150 (MQ=255) aaaaGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAAAATGAATATATTCCCGCG‑CTT‑AATACCACGcc > 2:1936841/1‑150 (MQ=255) aaaGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATGAATATATTCCCGCG‑CCT‑TATGCCACGccc > 1:143258/1‑149 (MQ=255) gTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACat < 2:3417187/150‑1 (MQ=255) gTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACat < 2:2846744/150‑1 (MQ=255) aGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCCGCG‑CTT‑AATTCCACGCCGGAACatat > 1:2952699/1‑150 (MQ=255) gTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATGAATATATTTCGGCG‑CTT‑TATGCCACGCCGGAACATATCg > 1:1355276/1‑150 (MQ=255) gTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATGAATATATTTCGGCG‑CTT‑AATGCCACGCCGGGACaaaacg > 2:4227428/1‑145 (MQ=255) gTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCg < 2:37426/150‑1 (MQ=255) cATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATAGAAATATTTCCGCG‑CTT‑TATGCCACAGCGGGAAATATCgta > 2:2881018/1‑148 (MQ=255) cATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CCT‑AATGCCACGCCGGGACATATCGaa > 1:668762/1‑150 (MQ=255) tatTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAAAATGAATATATTCCGGCG‑CTT‑AATGCCACCCCGGAACATATCGAAat > 1:3434730/1‑150 (MQ=255) atTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATGAATATATTTCGGCG‑CTT‑AATGCCACGCCGGGACATATCGaaaag > 2:2558744/1‑148 (MQ=255) atTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAAAATATTCCTGCG‑CTT‑TATACCCAACCGGAAAATATTGAAatg > 1:4091132/1‑150 (MQ=255) ttCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGAATATATTTCCGCG‑CTT‑AATGCCACGCCGGGACATATCGAAatga > 1:99373/1‑150 (MQ=255) cAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAGACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAatgatg < 1:2373026/150‑1 (MQ=255) cAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAatgatg < 1:424389/150‑1 (MQ=255) aGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATgg < 1:1218581/150‑1 (MQ=255) gTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATGAAAATATTTCCGGG‑CTTCAATG‑CACACCCGGACATATCGAAAAGATggat > 2:2733550/1‑148 (MQ=255) gTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCt < 1:2184080/150‑1 (MQ=255) gATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATGAAAATATTTCCGCG‑CTT‑AATGCCACGCCCGGACATATCGAAATGAAGGGTaa > 2:4121828/1‑150 (MQ=255) aTGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAAt < 1:2655595/150‑1 (MQ=255) gtgCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCt < 2:2915866/150‑1 (MQ=255) tgCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCtt < 2:3252553/150‑1 (MQ=255) tgCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCtt < 1:954534/150‑1 (MQ=255) ccGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCttgtt < 2:4229702/150‑1 (MQ=255) cGGGCTTTGCTGCGGATTGNCTGGAGNNGNNGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCttgttg < 1:1608587/150‑1 (MQ=255) ggCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGcc < 1:180309/150‑1 (MQ=255) ttGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAAAGAATATATTTCGGGG‑CTT‑TATGCCACGCCGGGACAAATCGAAATGATGGGTAATCTTTTTGCcgcgg > 2:1705496/1‑149 (MQ=255) tgctgcGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTa < 1:740350/150‑1 (MQ=255) tgctgcGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTa < 1:3517624/150‑1 (MQ=255) gctgcGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTAt < 2:2579119/150‑1 (MQ=255) ctgcGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATc < 2:3666789/150‑1 (MQ=255) cGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCt < 2:4415059/150‑1 (MQ=255) ggATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCCCGCCGGGAAATAACGAAAAGATGGGTAATTCTGTTGCCGCGGATCGCTa > 2:705556/1‑150 (MQ=255) ggATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATGAATATATTTCGGCG‑CTT‑AATGCCCCGCCGGGAAATATCGAAATGATGGGTAATCTTTTTGCCCCGTATCCCTa > 2:3910150/1‑150 (MQ=255) aTTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTaaa < 2:2113358/150‑1 (MQ=255) cTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGa < 1:3222787/150‑1 (MQ=255) agaCGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCgg < 1:37403/150‑1 (MQ=255) ggAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGaa < 2:913454/150‑1 (MQ=255) gAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAAc < 2:4139804/150‑1 (MQ=255) agaTTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAATGAAAATATTTCCGGG‑CGT‑AATTCCACGCCGGGACATATCGAAATGATGGCGAATTTTTTTGCCGCGGGTCGCTAAAGCTGAGCGGGAAAGAAATGAg > 2:63106/1‑150 (MQ=255) gaTTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAgc < 1:3095421/150‑1 (MQ=255) tGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGcc < 2:2189208/150‑1 (MQ=255) ccGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGt < 1:3058288/150‑1 (MQ=255) ccGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGt < 2:1440909/150‑1 (MQ=255) cGAGCAAAACCGTGAGGTCTTCCCCGGTGCCGGCGGGAAAAAATATGAATATATTTCCGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTc > 1:2645596/1‑150 (MQ=255) aaaaCCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAACTGAGCCGTAAAGAACTGAGCGCCGTCGCGaa > 1:21447/1‑150 (MQ=255) aaaCCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAAAAAGAAAATATTCCCGCG‑CCT‑AATGCCACCCCGGGACAAATCGAAATGATGGCTAAACTTTTTGCCGCGGATCGCCAAAACTGGGCGGGAAAAAAATTAGGGCCCGCCCGAag > 2:4025758/1‑150 (MQ=255) aaaCCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAag < 2:3680431/150‑1 (MQ=255) aaCCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAaga < 1:375448/150‑1 (MQ=255) aCCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAAAATGAAAATATTTCGGCG‑CTT‑AATGCCCCGCCCGGACATAACGAAATGATGGCTAATTTTTTTGCCGCGGATCCCTAAAACTGAGAGGGAAAGAACAGAGCGCCCTCGCGAaaag > 2:11267/1‑150 (MQ=255) cGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAAAATGAATATATTTCGGGG‑CTT‑AATGCCACGCCGGGACAAATCGAAATGATGGGTAATCTTTTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAAAACTGAGGGCCGTCGCGAagaagc > 2:2328413/1‑147 (MQ=255) tGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCtc < 2:387803/150‑1 (MQ=255) tGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCtc < 1:3109646/150‑1 (MQ=255) tGAGGGCTTCCTGGGTGCGGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATCTCGAAATGATGGCTCATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCtc < 2:218143/150‑1 (MQ=255) ggTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCtcgtc < 2:3008646/150‑1 (MQ=255) ggTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCtcgtc < 1:3827730/150‑1 (MQ=255) gTCTTCCTCGGTGCCGGCGGGAAAAAAAATGAATATATTCCGGCG‑CTT‑TATGCCACGCCGGGACATATCGAAATGATGGCTAAACTTTTTGCCGCGTATCGCTAAAGCTGAGCGGGAAAGAACTGAGCGCCGTCGCGAAGAGCCtcgtcg > 2:1557439/1‑150 (MQ=255) tCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTcgg < 1:3455091/150‑1 (MQ=255) ttCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTcggcg > 1:431383/1‑150 (MQ=255) cTCGGTGCCGGCGGGAAAAAAAATGAAAATATTCCGGCG‑CTT‑AATGCCCCGCCGGGACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAAATGAGCGCCGTCGCGAAGAGCCTCGTcggcgggt > 2:3525227/1‑148 (MQ=255) cGGTGCCGGCGGGAAAAAAAAAAAAAAAATTTCGGGG‑CTT‑AAATCCCCGCCGGGACATATCGGAAAGATGGGTAATCTTTTTGGCGCGTATCGCTAAAACCTAGCGGGAAAGAACTGAGCGCCGTCGCGAAGAGACTCCTcgggggggtt > 2:1304/1‑146 (MQ=255) gTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTtca < 1:1120142/150‑1 (MQ=255) cggcggGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTcatcat < 2:819122/150‑1 (MQ=255) gcggGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATcc < 1:486012/150‑1 (MQ=255) aaaaaaTATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGaa > 1:3959298/1‑150 (MQ=255) aaaaaaTATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGaa < 2:1840529/150‑1 (MQ=255) | GCCTTATACCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCG‑CTT‑AATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAA > AP012306/406529‑406826 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |