Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,182,5060AC21.0% 244.8 / 10.8 100D80A (GAC→GCC) yiaNpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (44/35);  new base C (0/21);  total (44/56)
Fisher's exact test for biased strand distribution p-value = 7.09e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 188 total aligned reads displayed.

GTTAATGTTCTGGCTGGACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCC  >  AP012306/3182357‑3182654
                                                                                                                                                        |                                                                                                                                                     
gTTAATGTTCTGGCTGGACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGa                                                                                                                                                       <  1:1246271/150‑1 (MQ=255)
gTTAATGTTCTGGCTGGACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGa                                                                                                                                                       <  1:702849/150‑1 (MQ=255)
  tAATGTTCTGGCTGGACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGGGAAAACATGAATGC‑GGGGGGCGTGTCAAAACGCATTGTTGGcc                                                                                                                                                     >  2:1119726/1‑150 (MQ=255)
   aaTGTTCTGGCTGGACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGGGGCTTGTCCAAAAGCATTGTTTACCt                                                                                                                                                    >  1:1282632/1‑150 (MQ=255)
   aaTGTTCTGGCTGGACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGGGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTTTTTACCt                                                                                                                                                    >  1:3421288/1‑150 (MQ=255)
   aaTGTTCTGGCTGGACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGGGAAATCATGAATGC‑GGGCGGCCTTTCAAAAAGCATTGTTGGccc                                                                                                                                                    >  2:6357/1‑149 (MQ=255)
    aTGTTCTGGCTGGACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTg                                                                                                                                                   <  2:4193608/150‑1 (MQ=255)
     tGTTCTGGCTGGACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGGGGAATAATGAAAGC‑GGGCCGCTTGTCAAAACGCATTTTTGACCCGc                                                                                                                                                  >  2:1646743/1‑150 (MQ=255)
      gTTCTGGCTGGACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGcc                                                                                                                                                 <  2:512880/150‑1 (MQ=255)
      gTTCTGGCTGGACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGcc                                                                                                                                                 <  1:2300222/150‑1 (MQ=255)
       ttCTGGCTGGACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGCCg                                                                                                                                                <  2:18239/150‑1 (MQ=255)
       ttCTGGCTGGACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAAAGC‑GGGCGGCTTGTCAAAAACCATTGTTTACCCGccc                                                                                                                                                >  1:2447029/1‑149 (MQ=255)
        tCTGGCTGGACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGa                                                                                                                                               <  1:3301891/150‑1 (MQ=255)
           ggctggACATTTTTGATGCCCAGATAATGGCAAAACCGCGGGTGAAGGGAGCGGATAGCTTCTCCCTGCTGGCGTTTCCGTTCTTT‑GT‑GCGGGGGGGTGAAATCATGAATGG‑GGGCGGTTTGTCAAAACGCTTTGTGGCCCTGCCGAT‑Ga                                                                                                                                           <  2:4168950/150‑1 (MQ=255)
             ctggACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGAT‑Gaaa                                                                                                                                         <  1:2729682/150‑1 (MQ=255)
              tggACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGTGAAAACATGAATGC‑GGGCGGGCTGTCAAAAAGCCTTGTTTACCTGCCGCT‑Gaaaa                                                                                                                                        >  2:2408350/1‑149 (MQ=255)
              tggACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGGGGAAACATGAATGC‑GGGGGGCGTGTCAAAAAGCAATGTTGACCCGCCGAT‑Ggaaa                                                                                                                                        >  2:4252662/1‑149 (MQ=255)
               ggACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAAAGCATTGTTGACCTGCCGAT‑GAAACt                                                                                                                                       >  1:1464507/1‑150 (MQ=255)
               ggACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGGGGAATCATTAATGC‑GGGGGGCTTTTTCAAACGCATTGTTGACCCGCCGCT‑Gaaaaa                                                                                                                                       >  2:3678276/1‑148 (MQ=255)
               ggACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGGGGAATCATGAAAGC‑GGGCGGGGTGTCAAAACCCCTTGTTTACCTTCCGAT‑GAAACt                                                                                                                                       >  1:1652224/1‑150 (MQ=255)
                gACATGTTTGATGTCGAGATCAGGGCAAAAACGTTGGTGAACGGAGCCGAAAGCTTCTCCCTGTTGGGGATTCCTTTGTTT‑GG‑GCTGGGGGTGGAAATAAGGAATGC‑GGGCGGCTTAAAAAAGCGATTTTTTGACCTGCCGAT‑GAAACtg                                                                                                                                      <  2:262706/150‑1 (MQ=255)
                gACATGTTTGATGTCCAGATCATGGCACAACCGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGAT‑GAAACtg                                                                                                                                      <  1:885969/150‑1 (MQ=255)
                   atttttGATGTCCAGATCATGGCACAAACGCTGGTGAAGGGAGCCGATAGCTTCTCCCTGCTGGCGTTTCCGTTTTTT‑GT‑GCTGGCGGGTGAAATCATGAATGG‑GGGGGGCTTGTAAAAACGCATTGTTGACCTGCCGAT‑GAAACtggtg                                                                                                                                   <  2:2939062/147‑1 (MQ=255)
                     gTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGAT‑GAAACTGGTggg                                                                                                                                 <  1:3333316/150‑1 (MQ=255)
                     gTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCCCCCTGCTGGCGTTTCCGTTTTTT‑GT‑GCTGGGGGGTGAAATCATGAAGGC‑GGGCGGCTTGTAAAACCGCATTGTTGCCCTGCCGAT‑GAAACTGGTggg                                                                                                                                 <  2:3083750/150‑1 (MQ=255)
                         gATGTCCAGATCATGGCACAAACGCTGGTGAACGGACCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGGGAAAACATGAAATC‑GGGGGGCTTTTCAAAAAGCACTGTTTACCTCCCGCT‑GAAACTTgggggggct                                                                                                                             >  2:3858662/1‑147 (MQ=255)
                               aaGATCATGGCACAAACGCGGGTGAAGGGAGCCGATAGTTTCTCCCTGCGGGCGATTCCTTTTTTT‑GT‑GCTGGGGGGTGAAATCATGAAGGG‑GGGCGGTTTGTAAAAACGCTTTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCa                                                                                                                       <  2:3155457/149‑1 (MQ=255)
                                  aTCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGc                                                                                                                    <  1:310421/150‑1 (MQ=255)
                                   tCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCTTTTTTT‑GT‑GCTGGGGGGTGAAATCATGAATGC‑GGGGGGTTTGTAAAAACGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCg                                                                                                                   <  1:1342777/150‑1 (MQ=255)
                                   tCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGGGAAATCATGAATGC‑GGGGGGCTTGTCAAAAAGCATTGTTGACCTGCCGCT‑GAAACTGGTGGGGGGTAAACCAGGCg                                                                                                                   >  2:3315555/1‑150 (MQ=255)
                                    cATGGCACAACCGCTGGTGAACGGAGCCGATAGCTTCCCCCTGCTGGCGATTCCGTTTTTT‑GT‑GCTGGGGGGTGAAATCATGAAGGC‑GGGCGGCTTGAAAAAACGCTTTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCgg                                                                                                                  <  2:3865133/150‑1 (MQ=255)
                                    cATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGGGGGTGAAATCATGAATGC‑GGGCGGCTTGAAAAAACGCTTTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCgg                                                                                                                  <  2:1977061/150‑1 (MQ=255)
                                    cATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGGGGAAACATGAATGC‑GGGGGGCCTGTCCAAAAGCCATGTTTGCCTGCCGCTGGAAACGGGGGGGGCGAAAACAAgcgg                                                                                                                   >  2:2031501/1‑147 (MQ=255)
                                       ggaacaAGCGTTGGTGAAGGAAGCCAATCGTTTCCCCCTGCTGGCAATTCTTTTTTTG‑GT‑GCGGGGGGGGAAAACCATGTACGG‑GGGGGGCTGGTAAAACCGAGTTGGCGGCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCgggct                                                                                                               <  2:3222405/147‑1 (MQ=255)
                                       gccacaACCGCGGGTGAAGGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCTTTTTTT‑GT‑GCTGGCGGGTAAAATCATGAATGC‑GGGCGGCTTGTAAAAACGCATTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCgggct                                                                                                               <  2:3659845/148‑1 (MQ=255)
                                           caacggttGGTGATGGGCGCCGATAGCTTCCCCCTGTTGGGGATCCCGTTTTTT‑GT‑GCTGGGGGTAGAAATTATGAATGC‑GGGCGGCTTGAAAAAACGCTTTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCgggctgggc                                                                                                           <  2:2820654/143‑1 (MQ=255)
                                           caACCGCGGGTGAAGGGGGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTTTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTAAAAACGCTTTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCgggctgggc                                                                                                           <  1:3352900/150‑1 (MQ=255)
                                            aaaCGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGTGGAATCAAAAATACGGGGGGGCTTGT‑AAAACGCATCGTTGGCCTGGCCGT‑GAAAAATGGGGGGGATAAACAACGCgggcgtgggt                                                                                                          >  2:590903/1‑144 (MQ=255)
                                            aaaCGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGGGGAAACATGAATGC‑GGGCGGCGTGTCAAAACGCATTGTTGACCTGCCGGT‑GGAACTGGGGGGGGGTAAACCAGGCgggggggggg                                                                                                          >  2:2795909/1‑143 (MQ=255)
                                                 ttGGGGAACGGAGCCGATAGCTCCTCCGTGCTGGCGGTTCCGTTTTTT‑GT‑GGTGGCGGGTAAAATCATGAAGGC‑GGGGGGTTTGTCAAAGCGATTTGTGGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTc                                                                                                     <  2:377380/149‑1 (MQ=255)
                                                 cTGGTAAAGGGAGCCGATAGCTTCCCCCTGCTGGCGATTCCTTTTTTT‑GT‑GCTGGCGGGTGAAATCATGAATGG‑GGGCGGCTTGTAAAAACGCTTTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTc                                                                                                     <  2:211318/150‑1 (MQ=255)
                                                   ggTGAACGGAGCCGATAGCTTCTCCCTGCGGGCGTTTCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAACCGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCgg                                                                                                   <  1:2888963/150‑1 (MQ=255)
                                                    gTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGAGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGc                                                                                                  <  1:1205924/150‑1 (MQ=255)
                                                     tGAAGGGAGCAGATAGTTTCTCCTTGCTGGTAATTCCTTTTTTT‑GT‑GCTGGCGGGTAAAATCATGAAGGC‑GGGCGGCTTGTCAAACCGCTTTGTTTCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCg                                                                                                 <  2:2569736/150‑1 (MQ=255)
                                                      gAAGGGACCCGATACTTCCTCCCGGCTGGCGATTCCGTTTTTT‑GG‑GCTGGCGGGTAAAATCATGAAGGG‑GGGCGGCTTGTCAAAACGCATTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGt                                                                                                <  2:2172132/150‑1 (MQ=255)
                                                           gAGCCGATACCTTCCCCCTGCTGGCGATTCCGTTTTTT‑GT‑GCTGGGGGGTAAAATCATGAATGC‑GGGGGGTTTGTCAAAACCCTTTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTgg                                                                                           <  2:1714008/150‑1 (MQ=255)
                                                             gcgtttggCTTCTCCTTGTGGGAGTTTCTTTTCTTT‑GT‑GCGGGGGGGTAAAATAATGAAGGG‑GGGGGTTTTGTAAAAACGCTTTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCa                                                                                         <  2:1379151/143‑1 (MQ=255)
                                                               cGATAGCTTCCCCCTGCGGGCGTTTCCTTTCTTT‑GT‑GCGGGCGGGTAAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGc                                                                                       <  2:2242923/150‑1 (MQ=255)
                                                               cGATACCTCCTCCCTGCTGGCGTTCCCGTTCTTT‑GT‑GCGGGGGGGTGAAATCAGGAGTGC‑GGGCGGCTTGTCAAACCGCATTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGc                                                                                       <  2:2801089/150‑1 (MQ=255)
                                                                gatgccTTCTCCGTGCGGGCG‑TTCCTTTTTTTGGT‑GGGGGCGGTTGAAATCAGGAAGGC‑GGGCGGTTTGTCAAACCGCTTTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCt                                                                                      <  2:3457622/145‑1 (MQ=255)
                                                                 attgtttCCCCCTGCTGGCGATTCTGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGTTTGTAAAACCGCTTTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTa                                                                                     <  2:160278/145‑1 (MQ=255)
                                                                 aTAGCTTCCCCCGGCGGGCGATTCCGTTTTTT‑GT‑GCGGGGGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTa                                                                                     <  2:4056339/150‑1 (MQ=255)
                                                                   aGCTTCTCCCTGCTGGCTTTTCGTTTCTTT‑GT‑GCGGGGGGGTGAAATCATGAATGC‑GGGCGGTTTGTAAAACCGCTTTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATg                                                                                   <  2:2900549/150‑1 (MQ=255)
                                                                   aGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGGGAAAATATGAATGC‑GGGGGGCGTGTCAAAACACATTGTTTAACTGCCCAT‑GAAAAATGTAGGGGGTAAACCAAGCGGGAGGGGGTTCGTGGGGGGGCGGGGGGCTATg                                                                                   >  2:3114596/1‑150 (MQ=255)
                                                                     cTTCTCCTTGTTGATGATCCTTTTCTTT‑GC‑GCTGGCGGATAAAACAATGAAGGC‑GGGGGTCTTGCCAAACGGCATGTTTTTCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGAt                                                                                 <  2:3979849/150‑1 (MQ=255)
                                                                      ttCCCCTTGCGGGCG‑TTCCGTTTTTT‑TTGGCGGGGGGGGAAAATCATAAAGGG‑GGGCGTTTTGTAAAAACGCTTTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGAtt                                                                                <  2:4387493/150‑1 (MQ=255)
                                                                        ctcCCTGCTGGCGATTCCGTTTTTT‑GT‑GGTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAACCGCTTTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTAt                                                                              <  2:3941942/150‑1 (MQ=255)
                                                                                gggCTATTCTTTTTTTT‑GT‑GCGGGGGGGTAAAATCATGAAGGG‑GGGCGGCTTGTAAAAACGCATTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGcc                                                                      <  2:3515562/149‑1 (MQ=255)
                                                                                  gcggtcccGTTTTTT‑GT‑GGTGGGGGGTGAAATAATGAATGC‑GGGCGGTTTGTCAAAACGCATTGTTTCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCtt                                                                    <  2:3820987/144‑1 (MQ=255)
                                                                                   cGATTCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCTTTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCttt                                                                   <  1:2456576/150‑1 (MQ=255)
                                                                                       tCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTAAAAACGCATTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTgg                                                               <  1:1455545/150‑1 (MQ=255)
                                                                                         cGTTCTTG‑GT‑GCTGGCGGGTAAAATCATGAAGGC‑GGGCGGTTTGTAAAACCGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGtt                                                             <  1:2041526/150‑1 (MQ=255)
                                                                                          gTTTTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTc                                                            <  1:3548742/150‑1 (MQ=255)
                                                                                           ttCTTT‑GG‑GCGGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCt                                                           <  1:1982267/150‑1 (MQ=255)
                                                                                               tt‑GT‑GCTGGGGGGTGAAATCATGAATGG‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCg                                                       <  1:2095883/150‑1 (MQ=255)
                                                                                                   t‑gCGGGCGGGTAAAATCATGAATGG‑GGGCGGTTTGACAAAACGCTTTTTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTg                                                    <  2:18725/150‑1 (MQ=255)
                                                                                                      cTGGCGGGTGAAATCATGAAGGC‑GGGCGGCTTGTCAAAACGCTTTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCg                                                  <  1:2908250/150‑1 (MQ=255)
                                                                                                       gggggggTAAAATCATGAATGG‑GGGCGGTTTGTAAAAACGCTTTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCgg                                                 <  1:3815010/146‑1 (MQ=255)
                                                                                                       gggCGGGTGAAATTATGAATGG‑GGGCGGCTTGCAAAAACGTTTTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCgg                                                 <  1:1399326/149‑1 (MQ=255)
                                                                                                           gggTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAc                                             >  1:1847684/1‑150 (MQ=255)
                                                                                                            ggTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAcc                                            >  2:26090/1‑150 (MQ=255)
                                                                                                               gAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGAT‑GAAACTGGGGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGGTAccgcc                                         >  1:316046/1‑150 (MQ=255)
                                                                                                               gAAATCATGAAGGC‑GGGCGGCTTGTAAAAACGCTTTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgcc                                         <  1:645941/150‑1 (MQ=255)
                                                                                                               aaaaTCATGAATGC‑GGGGGGCTTGTCAAACCGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgcc                                         <  1:3841488/149‑1 (MQ=255)
                                                                                                                aaaTCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGCGGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGGTGGGCTACGGCGGGGGGCTGGGAGCTATGATTATGGCGAGCCTTTTTGGTTCTGGCGGTGGGGGTAccgccg                                        >  2:1215841/1‑150 (MQ=255)
                                                                                                                aaaTCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCATGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGGTCTGCCGTTGCGGATAccgccg                                        >  1:4223839/1‑150 (MQ=255)
                                                                                                                  aTCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATAccgccgcc                                      >  1:3975246/1‑150 (MQ=255)
                                                                                                                   tCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTTTTGTTCTTCCGTTTCGGGTAccgcccccc                                     >  2:4273848/1‑149 (MQ=255)
                                                                                                                     aTGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGGTACCGCCGCCGtt                                   >  2:2393914/1‑150 (MQ=255)
                                                                                                                        aaTGG‑GGGAGGTTTGAAAAAAGACTTTGTCCACCTGCCGAT‑GAAACTGGTGGGGCATACACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGcc                                <  2:907477/150‑1 (MQ=255)
                                                                                                                          aggggggcggTTTGTAAAAACGCATTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCg                               <  1:1570246/146‑1 (MQ=255)
                                                                                                                           ggggggcggCTTATAAAAGCGCTTTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGc                              <  1:3901051/147‑1 (MQ=255)
                                                                                                                                 cggtTTGTAAAACCGTTTTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCAGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACt                        <  2:147821/150‑1 (MQ=255)
                                                                                                                                  ggcTTGTCAAAACGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTg                       >  1:3561057/1‑150 (MQ=255)
                                                                                                                                   gcTTGTCAAAACGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGGCTTTCTGGTTCTTCCCTTGCGGATACCGCCGGCGTTGCCGCTCTACTgg                      >  2:4096448/1‑150 (MQ=255)
                                                                                                                                   gcTTGTCAAAACGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTgg                      >  1:3948463/1‑150 (MQ=255)
                                                                                                                                    cttgaaaaacCGCTTTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTATCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGt                     <  1:1930186/144‑1 (MQ=255)
                                                                                                                                         aaaacCGCTTTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCga                <  1:36062/149‑1 (MQ=255)
                                                                                                                                          aaaaCGTTTTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgat               <  1:3028202/150‑1 (MQ=255)
                                                                                                                                          aaaaCGCATTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgat               <  1:3618598/150‑1 (MQ=255)
                                                                                                                                           aaaCGCTTTGTTGCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCgatg              <  1:1740267/150‑1 (MQ=255)
                                                                                                                                            aaCGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGGTGCCGCTCTTCTTGTGCCggtta             >  2:2297470/1‑146 (MQ=255)
                                                                                                                                              cGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTTGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTTGTTCTGCCGTTTCGGGTACCCCCGCCGTTGGCGCCCTACTGGTGCCGgtgatg           >  2:1726414/1‑150 (MQ=255)
                                                                                                                                                 tttgttgCCCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATgcgc        <  2:3589507/149‑1 (MQ=255)
                                                                                                                                                 aTTGTGGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATgcgc        <  1:894232/150‑1 (MQ=255)
                                                                                                                                                   tgttgACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGGTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGGTACCGCCGCCGCTGCCCCCCTACTGGTGCCGCTGGTGCGCAg      >  2:963723/1‑150 (MQ=255)
                                                                                                                                                    gttgACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAgc     >  1:2676575/1‑150 (MQ=255)
                                                                                                                                                    gtcgACCTGCCGAT‑GAAACTGGTGGGGCATAACCCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAgc     <  1:935425/147‑1 (MQ=255)
                                                                                                                                                       gACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGGGCCGATGTTTCGCAGCGcc  >  2:3369042/1‑150 (MQ=255)
                                                                                                                                                       gACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGGTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGGTGATGCGCAGCGcc  >  2:713926/1‑150 (MQ=255)
                                                                                                                                                        |                                                                                                                                                     
GTTAATGTTCTGGCTGGACATGTTTGATGTCCAGATCATGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTT‑GT‑GCTGGCGGGTGAAATCATGAATGC‑GGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGAT‑GAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTTTCTGGTTCTGCCGTTGCGGATACCGCCGCCGTTGCCGCTCTACTGGTGCCGATGATGCGCAGCGCC  >  AP012306/3182357‑3182654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑