Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,182,8610AC31.0% 336.5 / 39.3 155L198F (TTA→TTC) yiaNpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (92/15);  new base C (0/48);  total (92/63)
Fisher's exact test for biased strand distribution p-value = 3.88e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 231 total aligned reads displayed.

ATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGC  >  AP012306/3182712‑3183010
                                                                                                                                                        |                                                                                                                                                      
 ttattCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTAc                                                                                                                                                       <  2:1883836/150‑1 (MQ=255)
  tattCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTAcc                                                                                                                                                      <  2:1263613/150‑1 (MQ=255)
    ttCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCGGTGTCCCCCGCCTGATTATGGGGGCAA‑ACCTGGTGGTTTCCTTGTGGGGGGGGGGCAG‑GCGCC‑TTTTTTTTcccc                                                                                                                                                    >  2:401143/1‑150 (MQ=255)
     tCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGCGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCg                                                                                                                                                   <  2:3488230/150‑1 (MQ=255)
        cccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCa                                                                                                                                                <  1:2585460/150‑1 (MQ=255)
         ccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCAATTCCCCCCGCCGGATTTTAGGGGCAA‑CGCCTATGGTTTCCTGGTGGTGGGCGGGCAG‑TTGCC‑CTCATTTATcccccccg                                                                                                                                               >  2:1691568/1‑148 (MQ=255)
          cTTCCATCCCTTTTTTTATCTTCGGCTTTTCCAGCGGATTATCCATCAGTAAGCTTTTTATGGGGGGCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTCCCTGGGGGTGGCAGGCCAG‑TCGCC‑TTAATTTCCCCCGCCAGc                                                                                                                                              <  2:1788992/150‑1 (MQ=255)
             ccATTCCGTTTATTATCTTGGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTCCCTGGTGGGGGCAGGCCAG‑TCGCC‑TTAATTTCCCCCGCCAGCaaa                                                                                                                                           <  2:2689489/150‑1 (MQ=255)
              cATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCaaaa                                                                                                                                          <  1:758098/150‑1 (MQ=255)
                ttCCTTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTCCCCCGCCAGCAAAAAg                                                                                                                                        <  2:2567608/150‑1 (MQ=255)
                ttCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCTCGCATGATGATGGGGGCCA‑CGCTGATGCTTTCCTGGTGGGGGCAGGCCAG‑TCGCC‑CTAATTTATCCCCCCCGCcaaaaa                                                                                                                                        >  2:3174889/1‑149 (MQ=255)
                 tCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGc                                                                                                                                       <  1:2589894/150‑1 (MQ=255)
                    gTTTTTTATCTTCGGCGTTCCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTCCCTGGGGGGGGCAGGCCAG‑TCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAAc                                                                                                                                    <  2:685462/150‑1 (MQ=255)
                     tttATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGCTTTCCCCCGGCCTGGTGGTGGGGGCCA‑CGCTGATGGTTACCTGGGGGGGGGGGGCCAG‑TCGCCTTTAATTTTCCCC‑CCCGCaaaaaaacaag                                                                                                                                   >  2:898215/1‑144 (MQ=255)
                     tttATTATCTTCGGCGTTCCCAGCGGATTATCCATCAGAAAGCTGTTTTTGGCGGGCTTTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTCCCGGGTGGGGGCGGGCCAG‑TCGCT‑TTAATTTCCCCCGCCAGCAAAAAGCAACg                                                                                                                                   <  2:2326967/150‑1 (MQ=255)
                      ttattaTCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTGCCCCGGCCTGATGATGGGGGCAACCGCCG‑TGCTTATCTTTGGGGGGGAGGCCAG‑GCCGC‑TTAATTTACCCCCCCCGCaaaaaaaaaaca                                                                                                                                  >  2:814277/1‑143 (MQ=255)
                       tattatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGACGGGCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGAt                                                                                                                                 >  1:3174339/1‑150 (MQ=255)
                       tattacCTCCGGCGTTCCCAGCGGATTACCCATCAGTAAGTTGTTTATGGCGGGCATTGCCCCTGGCATGATGAGGGGCGCAC‑CCCTGATCCTTCCCGGGGGGTGGCGGGCCGG‑TCGCT‑TTATTTTCCCCCGCCAGCAAAAAGCAACGAt                                                                                                                                 <  2:2033483/150‑1 (MQ=255)
                        attatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCCGCATGATTATGGGGGGCA‑AGCTGATGGTTACCTTGTGGGGGGCGGGCAG‑GCGCC‑TTTATTTACCCCCCCCGCCAAAAAAAAAGATg                                                                                                                                >  2:2201704/1‑150 (MQ=255)
                        attatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTAGGGCGGGCTTTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGGGGGGGCAGGCCAG‑TCGCT‑TTATTTTCCCCCGCCAGCAAAAAGCAACGATg                                                                                                                                <  2:2618047/150‑1 (MQ=255)
                          tatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑a                                                                                                                             <  1:3556228/150‑1 (MQ=255)
                           atCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGGGGAA‑CGCTGATGCTTAACTGGTGGTGGCAGGCCAG‑TCGCC‑CTAATTTACCCCGCCAGCAAAAAGCAAAGAAGC‑aa                                                                                                                            >  2:3742740/1‑149 (MQ=255)
                                  gCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCCGCCTGGTGATTGGGGCCA‑CGCCCATGCCTTCCCGGGGGGGGCAGGCCCA‑GCGCC‑CTAATTTACCCCCCCCGCCAAAAACAACGAAGCAAGGaaaat                                                                                                                      >  2:933074/1‑148 (MQ=255)
                                   cGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCCGCCTGATGATGGGGGCAA‑CGCTGATGCTTTCCTGGTGGTGGGGGGGCAG‑TCGCC‑CTTATTTTTCCCCCCCGCCAAAAACAAAAAAGC‑AGGgaaaatt                                                                                                                    >  2:3296819/1‑146 (MQ=255)
                                    ttttCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCTTGGCATGATGGGGGGCGCAC‑CGCGGATGCTTACCTGGTGGTGGCGGGCCGG‑TCGCT‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTgg                                                                                                                   <  2:2833181/149‑1 (MQ=255)
                                    gTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGGTTTCCCCCTGCAGCATGGTGGGGGCAA‑CGCTGATGTCTACCTGGTGGGGGCGCGGCAG‑GGGCC‑TTAATTTTTCCCCCCCGCAAAAAGCAAAGATGC‑CGGAAAACTgg                                                                                                                   >  2:3951282/1‑150 (MQ=255)
                                         cAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑CCGCT‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCActc                                                                                                              <  1:1511554/150‑1 (MQ=255)
                                          aGCGGATTACCCATCAGTAAGCTGTTTAGGGCGGGCTTTCCCCCTGGCATGATGATGGGCGCAC‑CGCTGATGCTTACCTGGTGGGGGCGGGCCAG‑CCGCT‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCActct                                                                                                             <  2:4274096/150‑1 (MQ=255)
                                           gCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGAGGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCT‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCtt                                                                                                            <  1:167267/150‑1 (MQ=255)
                                             ggATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCCGGCATGATGATGGGGGCAA‑CGCTGATGCTTACCTCGGGGGGGGAGGCCAG‑TCGCC‑TTAATTTTCCCCCCCCGCCAAAAACAACGAAGC‑AGGCAAACCTGCACTCtttt                                                                                                          >  2:2508340/1‑150 (MQ=255)
                                               aTTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGt                                                                                                        >  1:493115/1‑150 (MQ=255)
                                                 tATCCATCAGTAAGCTGTTTATGGCGGGGCTTGCCCCCCGCCTGATGGTGGGGGCAA‑CGCTGATGGTTTCCTGGGGGGGGGAGGGCAG‑GCGCC‑TTTATTTACCCCCCCACCAAAAAAAAACGGTGC‑AGGGAAACTGGGACCCtttttttt                                                                                                      >  2:438663/1‑146 (MQ=255)
                                                 tATCCATCAGTAAGCTGTTTATGGCGGGCCTTGCCCCTGCCGCCATGGTTGGGGCGA‑CGCTTCAGCGTTGCCGGTGGGGGGCGGCCAG‑TCGGC‑CTTATTTAACCCCCCCGCAAAAAAAAACAATGG‑AGGGAATGTGGGACCCtttttttt                                                                                                      >  2:2888065/1‑146 (MQ=255)
                                                     cacaggTAAGCTTTTTGGGGGGGGCATGGCCCGGGAAATGAGGGGGGGCGCAC‑GGCTGATGCTTCCCTGG‑GGGGGCCGGCCCGTTCCCC‑TTATTTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCgg                                                                                                  <  2:4421219/145‑1 (MQ=255)
                                                     cATCAGTAAGCTGTTTATGGGGGGCTTTGCCCCGGGCATGATGAGGGGCGCAA‑CGCTGATGCTTACCTGGGGGTGGCGGGCCAG‑TCGCT‑TTATTTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCgg                                                                                                  <  2:622388/150‑1 (MQ=255)
                                                       tCAGTAAGCTGTTTAGGGCGGGCATTGCCCCGGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGGGGGGGCAGGCCAG‑TCCCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTa                                                                                                <  2:210514/150‑1 (MQ=255)
                                                            aaGCTGTTTATGGCGGGCATTGCCCTTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCT‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTgg                                                                                           <  1:4267533/150‑1 (MQ=255)
                                                            aaGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTgg                                                                                           <  1:3355407/150‑1 (MQ=255)
                                                             ggCTGTTTATGGCGGGCTTTCCCCCTGGAATGATGATGGGCGCAC‑CGTTGATGTTTACCGGGGGGTGGCAGGCCAG‑TCGCT‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTggg                                                                                          <  2:2076554/149‑1 (MQ=255)
                                                                tGTTTATGGCGGGCATTGCCCCCGGCATGATGATGGGGGCAA‑CGCTTATGGTTTCCTGGTGGGGGGGGGGCAG‑TCGCC‑TTTATTTACCCCGCCCCCCAAAAGCAACGATGC‑AGGGAAACTTGCACTCTTTTTTCTCCCGGATCTGGgcgc                                                                                       >  2:1637672/1‑150 (MQ=255)
                                                                  tttAGGGCGGGCATTGCCCCTGGAATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTg                                                                                     <  1:1293523/150‑1 (MQ=255)
                                                                      tGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttc                                                                                 >  2:2755190/1‑150 (MQ=255)
                                                                        gCGGGCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttctt                                                                               <  1:877590/150‑1 (MQ=255)
                                                                            gCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAg                                                                           >  2:1535267/1‑150 (MQ=255)
                                                                             cATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGt                                                                          >  2:708859/1‑150 (MQ=255)
                                                                               ttGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtca                                                                        <  1:1216830/150‑1 (MQ=255)
                                                                                 gCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatc                                                                      <  1:401365/150‑1 (MQ=255)
                                                                                 gCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatc                                                                      >  2:3370011/1‑150 (MQ=255)
                                                                                   cccTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTcatcat                                                                    >  2:1425042/1‑150 (MQ=255)
                                                                                       ggCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTACCAGTCATCattatt                                                                >  2:3650949/1‑150 (MQ=255)
                                                                                        gCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTg                                                               >  2:2941126/1‑150 (MQ=255)
                                                                                         cATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTgg                                                              >  1:982447/1‑150 (MQ=255)
                                                                                             atggtgGGCGCAA‑CGCTGATGCTTACGTGGTGGTGGCGGGCCAG‑TCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCggg                                                          <  2:1826498/150‑1 (MQ=255)
                                                                                             atgatgGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCggg                                                          >  2:127278/1‑150 (MQ=255)
                                                                                              tgatgGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCgggg                                                         >  2:1443993/1‑149 (MQ=255)
                                                                                              tgatgGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCgggg                                                         >  2:3512536/1‑149 (MQ=255)
                                                                                              tgatgGCCGCAC‑CGCTGATCTTTCCCGGGTGGTGGCAGGCCGG‑CCCCC‑TTATTTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGt                                                         <  2:371284/150‑1 (MQ=255)
                                                                                              tgaggGGCGCAG‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGt                                                         <  2:3581147/150‑1 (MQ=255)
                                                                                              tgaggGGCGAAA‑CGCTGATGCTTACCTGGTGGGGGCAGGCCAG‑TCGCT‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGt                                                         <  1:2707432/150‑1 (MQ=255)
                                                                                               gatgGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCgggcg                                                        >  1:3149302/1‑148 (MQ=255)
                                                                                               gatgGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGtt                                                        >  2:3127965/1‑150 (MQ=255)
                                                                                                atgGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGttt                                                       >  2:646975/1‑150 (MQ=255)
                                                                                                 tgGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTc                                                      <  1:3481377/150‑1 (MQ=255)
                                                                                                 tgGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGATTc                                                      >  2:2328200/1‑150 (MQ=255)
                                                                                                    gcgcAC‑CGCTGATGCTTGCCTGGTGGTGGCAGGCAGG‑TCGCT‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCt                                                   <  1:2667588/150‑1 (MQ=255)
                                                                                                      gcAC‑CGCTGATGCTTCCCTGGTGGTGGCGGGCCAG‑TCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTcc                                                 <  2:4123700/150‑1 (MQ=255)
                                                                                                        aaccgcgGATGCTTCCCTGGTGGTGGCAGCCCAG‑TCCCC‑TTAATTCCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCg                                                <  1:3672576/147‑1 (MQ=255)
                                                                                                         a‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCGGc                                              >  2:3793825/1‑150 (MQ=255)
                                                                                                           cGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGcc                                             <  1:2453431/150‑1 (MQ=255)
                                                                                                           cGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCCCTCCCGcc                                             >  2:491066/1‑150 (MQ=255)
                                                                                                            gCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCt                                            <  1:2894691/150‑1 (MQ=255)
                                                                                                            gCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCGGCCt                                            >  2:1389888/1‑150 (MQ=255)
                                                                                                               gATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCCGCCTGtt                                         >  1:430424/1‑150 (MQ=255)
                                                                                                                aTGCTTACCGGGGGGTGGCAGGCCAG‑TCCCT‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGttt                                        <  2:4323610/150‑1 (MQ=255)
                                                                                                                     tACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTCCGGCCCGCTTAcccc                                   >  1:465245/1‑150 (MQ=255)
                                                                                                                        cTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTAc                                <  2:2718988/150‑1 (MQ=255)
                                                                                                                         tggtggtggCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCTGGCCGTTTTTCCCCAcc                               >  1:1080899/1‑150 (MQ=255)
                                                                                                                         tggggggggCAGGCCAG‑TCCCC‑TTATCTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGTAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTAcc                               <  2:3642545/150‑1 (MQ=255)
                                                                                                                               gggCAGGCCGG‑TCGCC‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGcc                         <  1:1784860/149‑1 (MQ=255)
                                                                                                                                  cAGGCCAG‑TCTCT‑TTATTTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCggg                      <  2:3693044/150‑1 (MQ=255)
                                                                                                                                   gggccgg‑ccGCA‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCgggg                     <  2:739226/149‑1 (MQ=255)
                                                                                                                                        aG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTCCCGCCCGTTTTCCCCTCCCCAAACCCGGGCCGtt                >  2:2374134/1‑150 (MQ=255)
                                                                                                                                        aG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTCCCGCCCGTTTTCCCCCACCCCAGCCCGGGGCggt                >  1:3433203/1‑148 (MQ=255)
                                                                                                                                           tcccc‑TTATTGTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTtgc              <  1:4376070/147‑1 (MQ=255)
                                                                                                                                           tCGCT‑TTAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTtgc              <  2:1156198/150‑1 (MQ=255)
                                                                                                                                           tCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTCCGGCCCGTTTTTCCCCCCCCAAGCCCGGGCCGCtgc              >  1:1159531/1‑150 (MQ=255)
                                                                                                                                            cGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCCGTTTTTCCCCACCCAAACCCGGGCCgctgct             >  1:4408716/1‑150 (MQ=255)
                                                                                                                                            cGCC‑TTAATTCACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTtgct             <  1:2639722/150‑1 (MQ=255)
                                                                                                                                             gCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCCGTTTTTCCCCCCCGCAGCCCGGGGCGTtgctg            >  1:3917159/1‑150 (MQ=255)
                                                                                                                                              cc‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCTCCGGCCTGTTTACCCCTACCCAAGCCCGGGGCGGtgctgc           >  1:2849792/1‑150 (MQ=255)
                                                                                                                                                tttAATTTCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTgctgct          <  1:3985625/149‑1 (MQ=255)
                                                                                                                                                  tAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCTCTTTTTCCCCCCCCCAACCCGGGCCGGTGCTGCttt        >  2:4224630/1‑150 (MQ=255)
                                                                                                                                                  tAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCCGTTTTTCCCCCCCCCAGCCCGGGGCGCTGCTTCttt        >  2:1937281/1‑150 (MQ=255)
                                                                                                                                                  tAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCTCCCGCCTGTTTTCCCCCACCCCAGCCGGGGGCGGTGCTGCttt        >  1:2497395/1‑150 (MQ=255)
                                                                                                                                                    aTTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTCTCCCGCCTGTTTTTCCCCACCCCAGCCCGGGCCGGTGGTGCttttt      >  2:4131850/1‑148 (MQ=255)
                                                                                                                                                     tttCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTa     <  1:1378617/150‑1 (MQ=255)
                                                                                                                                                     tttCCCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTa     <  2:3987652/150‑1 (MQ=255)
                                                                                                                                                     tttACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTCCCGCCCGTTTTCCCCCACCCCAGCCGGGGGCGGTGCTGCTTTCTa     >  1:1011949/1‑150 (MQ=255)
                                                                                                                                                     tttACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCCGTTTTCCCCCCCCCCAGCCCGGGCCCGTGCTGCtttttt     >  1:214888/1‑147 (MQ=255)
                                                                                                                                                     tttACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCTCCCGCCCGGTTTCCCCCACCCAAACCCGGGCCGTTGCTGCcttttt     >  1:1713092/1‑147 (MQ=255)
                                                                                                                                                        |                                                                                                                                                      
ATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAA‑CGCTGATGCTTACCTGGTGGTGGCAGGCCAG‑TCGCC‑TTAATTTACCCCGCCAGCAAAAAGCAACGATGC‑AGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGC  >  AP012306/3182712‑3183010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑