Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,645,6420AC22.4% 888.5 / 25.5 336T318P (ACC→CCC) aceBmalate synthase A
Reads supporting (aligned to +/- strand):  ref base A (199/61);  new base C (0/75);  total (199/137)
Fisher's exact test for biased strand distribution p-value = 2.39e-37
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 372 total aligned reads displayed.

ATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCT  >  AP012306/3645493‑3645791
                                                                                                                                                      |                                                                                                                                                     
atatTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTaaaa                                                                                                                                                       <  2:699206/150‑1 (MQ=255)
   ttgttgGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCt                                                                                                                                                    <  1:1007149/150‑1 (MQ=255)
         gTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTACCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATa                                                                                                                                              <  2:1046992/150‑1 (MQ=255)
          tCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATaa                                                                                                                                             <  2:3893917/150‑1 (MQ=255)
          tCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATaa                                                                                                                                             >  1:2644237/1‑150 (MQ=255)
          tCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATaa                                                                                                                                             <  1:2366450/150‑1 (MQ=255)
           atGAACGGCGGTCGTTGGAATTACATCTTCAGTTATACAAAAAGGTTAAAAAACTCCCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAACCCATTCCGAAATGCTTCCTCCCCCCTGTTGATAAAACCCTGCCATaaa                                                                                                                                            <  2:294773/149‑1 (MQ=255)
            tGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAACCCATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAAc                                                                                                                                           <  2:4072374/150‑1 (MQ=255)
            tGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAAc                                                                                                                                           >  1:3442390/1‑150 (MQ=255)
             gAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAAcg                                                                                                                                          >  1:4198907/1‑150 (MQ=255)
              aaCTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAAcgc                                                                                                                                         >  2:2539630/1‑150 (MQ=255)
               aCTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAAcgcg                                                                                                                                        >  1:3626505/1‑150 (MQ=255)
               aCTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAAcgcg                                                                                                                                        >  1:1416544/1‑150 (MQ=255)
                     gccGTTGGGTTACCATCTTAAGCTATAACAAAACGTGGAAAAACTGTCCTG‑CCGCCGCCCGCCAAGACAGGCAGGGCGTACGGATGGAAAAGCTTTTCCGGAATGATTACTCCCGCCTGTTGATAAAACCCTGCCATAAACGCGGTGCtt                                                                                                                                  <  2:1685754/148‑1 (MQ=255)
                       cGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGAATAAACCATTCCTGAATGCTTACCCCCGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCtttt                                                                                                                                <  2:1924432/150‑1 (MQ=255)
                             gATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATg                                                                                                                          >  2:730856/1‑150 (MQ=255)
                              aTTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATgg                                                                                                                         >  2:46523/1‑150 (MQ=255)
                               ttACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATggg                                                                                                                        >  1:1671002/1‑150 (MQ=255)
                                    tCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCCCGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCa                                                                                                                   <  1:3035065/150‑1 (MQ=255)
                                    tCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCa                                                                                                                   >  2:3506135/1‑150 (MQ=255)
                                     cTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAACCCATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCAt                                                                                                                  <  1:547920/150‑1 (MQ=255)
                                     cTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCAt                                                                                                                  >  1:3179633/1‑150 (MQ=255)
                                      ttCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATg                                                                                                                 >  1:1653437/1‑150 (MQ=255)
                                        cAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATggc                                                                                                               >  2:3958887/1‑150 (MQ=255)
                                           cTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATggcggc                                                                                                            >  1:4393493/1‑150 (MQ=255)
                                            tataTCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGgcggcg                                                                                                           >  1:853249/1‑150 (MQ=255)
                                               atCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGttt                                                                                                        >  2:413584/1‑150 (MQ=255)
                                                  aaacCGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTAtt                                                                                                     <  1:2027003/150‑1 (MQ=255)
                                                  aaaaCGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTAtt                                                                                                     >  2:4186233/1‑150 (MQ=255)
                                                  aaaaCGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTAtt                                                                                                     >  1:3763215/1‑150 (MQ=255)
                                                     ccGTTAAAAAACAACCCCGACCGCCTCCTGCC‑AAAAAGACAGGAAGTGACGATGGATAAACCTTTCCTGAATGCTTCCTCGCGCTTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCg                                                                                                  <  2:1393485/149‑1 (MQ=255)
                                                     aCGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCg                                                                                                  >  2:3740870/1‑150 (MQ=255)
                                                     aCGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCg                                                                                                  >  1:2849964/1‑150 (MQ=255)
                                                     aCGTTGAAAAACTATCCCGATCGCGTCCGGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCg                                                                                                  <  1:1701150/150‑1 (MQ=255)
                                                      cGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGa                                                                                                 <  1:1102655/150‑1 (MQ=255)
                                                        ttGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGc                                                                                               <  1:3677356/150‑1 (MQ=255)
                                                        ttGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGATTATTCCGAGc                                                                                               >  2:3838450/1‑150 (MQ=255)
                                                        ttGAAAAACTACCCCGATCGCGCCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCTTTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGc                                                                                               <  2:661654/150‑1 (MQ=255)
                                                            aaaaCTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAg                                                                                           >  1:1733962/1‑150 (MQ=255)
                                                             aaaCTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGa                                                                                          >  1:4426863/1‑150 (MQ=255)
                                                               aCTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATg                                                                                        >  2:2821823/1‑150 (MQ=255)
                                                                 tATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGaa                                                                                      >  1:831890/1‑150 (MQ=255)
                                                                  aTCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGG‑GGCGTTTATTCCGAGCAAAGATGAaga                                                                                    >  1:476821/1‑150 (MQ=255)
                                                                   tCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAaga                                                                                    >  2:2346717/1‑150 (MQ=255)
                                                                   tCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAaga                                                                                    >  1:785421/1‑150 (MQ=255)
                                                                     ccGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGc                                                                                  >  1:1560655/1‑150 (MQ=255)
                                                                      cGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGca                                                                                 >  1:990896/1‑150 (MQ=255)
                                                                       gATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGcac                                                                                <  1:1837483/150‑1 (MQ=255)
                                                                        aTCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGTCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGcaca                                                                               >  2:2859170/1‑150 (MQ=255)
                                                                        aTCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGcaca                                                                               >  2:1813771/1‑150 (MQ=255)
                                                                               ccTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACCCCCGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCa                                                                        <  2:34200/150‑1 (MQ=255)
                                                                                cTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAg                                                                       >  2:1741638/1‑150 (MQ=255)
                                                                                cTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAg                                                                       >  1:2745772/1‑150 (MQ=255)
                                                                                  gCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGt                                                                     <  1:1922866/150‑1 (MQ=255)
                                                                                   cc‑AGACAGACAGGCAGTGACGATGGATAACCCATTCCTGAATGCTTACTCCCGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTg                                                                    <  1:1182310/150‑1 (MQ=255)
                                                                                    c‑agacagaCAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGc                                                                   >  2:2063465/1‑150 (MQ=255)
                                                                                      agacagacAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCt                                                                  >  2:1202654/1‑150 (MQ=255)
                                                                                      agacagacAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCt                                                                  <  1:3916234/150‑1 (MQ=255)
                                                                                       gacagacaGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTTAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTc                                                                 >  1:1590684/1‑150 (MQ=255)
                                                                                        acagacagGCAGTGACGATGGATAAACCATCCCTGAATGCTTCCCCCCGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTca                                                                <  2:2219771/150‑1 (MQ=255)
                                                                                            acagGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTcaacaa                                                            >  2:2627769/1‑150 (MQ=255)
                                                                                             caggaggTGACGAGGGATAAACCTTTCCAGAATGCTTACTCCCTCTTGTTGATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACaaa                                                           <  2:905025/144‑1 (MQ=255)
                                                                                               ggCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGt                                                         >  1:2922387/1‑150 (MQ=255)
                                                                                                gCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCCCGCCTGTTGATAAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTa                                                        <  2:526080/150‑1 (MQ=255)
                                                                                                   gTGACGATGGATAACCCATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTaaaa                                                     <  1:3356100/150‑1 (MQ=255)
                                                                                                   gTGACGATGGATAAACCATTCCTGAATGCTTACTCCCGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTaaaa                                                     <  1:4020572/150‑1 (MQ=255)
                                                                                                    tGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTaaagg                                                    >  2:3037946/1‑148 (MQ=255)
                                                                                                    tGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAg                                                    >  1:2402801/1‑150 (MQ=255)
                                                                                                    ggaggATGGATAACCCATCCGTAAATCCTGACCCCCGCTTATGGATAAAAGCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAg                                                    <  2:2762146/146‑1 (MQ=255)
                                                                                                       cGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCgg                                                 >  2:4420663/1‑150 (MQ=255)
                                                                                                        gATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGa                                                >  1:710810/1‑150 (MQ=255)
                                                                                                          tGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATa                                              >  1:2241243/1‑150 (MQ=255)
                                                                                                             aTAAACCATCCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAAt                                           <  1:4317479/150‑1 (MQ=255)
                                                                                                              tAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATc                                          <  2:2449187/150‑1 (MQ=255)
                                                                                                                aaCCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGc                                        >  2:2098827/1‑150 (MQ=255)
                                                                                                                  cctttCCTGAATGCTTACTCACGCTTGTTGATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTg                                      <  1:2138525/147‑1 (MQ=255)
                                                                                                                    aTTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGa                                    >  1:41205/1‑150 (MQ=255)
                                                                                                                    aTTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGa                                    >  2:2902562/1‑150 (MQ=255)
                                                                                                                    aTTCCTAAATGCTTACTCACGCCTGTTGATAAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGa                                    <  2:4335787/150‑1 (MQ=255)
                                                                                                                      tCCTGAATGCTTCCCCCCGCCTGTTAATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAg                                  <  2:2415179/150‑1 (MQ=255)
                                                                                                                      tCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAg                                  >  1:3140028/1‑150 (MQ=255)
                                                                                                                        cTGAATGCTTACTCCAGCTTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGcc                                <  2:3639120/150‑1 (MQ=255)
                                                                                                                        cTGAATGCTTACTCACGCCTGTAGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGcc                                <  1:1488075/150‑1 (MQ=255)
                                                                                                                             tGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATaa                           >  1:3307544/1‑150 (MQ=255)
                                                                                                                              gCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAAc                          <  1:643867/150‑1 (MQ=255)
                                                                                                                                ttACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACgg                        >  2:1453042/1‑150 (MQ=255)
                                                                                                                                 tACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGt                       >  1:2597623/1‑150 (MQ=255)
                                                                                                                                   cTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCa                     >  2:4254284/1‑150 (MQ=255)
                                                                                                                                      aCGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGa                  >  1:1271869/1‑150 (MQ=255)
                                                                                                                                          cTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGc              >  2:1308505/1‑150 (MQ=255)
                                                                                                                                           tGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGca             >  2:3322239/1‑150 (MQ=255)
                                                                                                                                           tGTTGATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGca             <  2:317396/150‑1 (MQ=255)
                                                                                                                                               gATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATg         <  2:787800/150‑1 (MQ=255)
                                                                                                                                                aTTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATgg        >  1:3703491/1‑150 (MQ=255)
                                                                                                                                                 ttAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGa       >  2:644004/1‑150 (MQ=255)
                                                                                                                                                  tAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGAt      <  2:4324049/150‑1 (MQ=255)
                                                                                                                                                  tAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGAt      >  1:307141/1‑150 (MQ=255)
                                                                                                                                                  tAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGAt      >  1:4165795/1‑150 (MQ=255)
                                                                                                                                                  tAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGAt      >  2:3252356/1‑150 (MQ=255)
                                                                                                                                                  aaaacCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGAt      <  2:3211163/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
ATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCC‑AGACAGACAGGCAGTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCT  >  AP012306/3645493‑3645791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑