BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	3,395,955	G→T	R109L (CGC→CTC)	rho →	transcription termination factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	3,395,955	0	G	T	100.0%	885.9 / NA	221	R109L (CGC→CTC)	rho	transcription termination factor
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (141/80); total (141/80)

Only 100 of 226 total aligned reads displayed.

ATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAA  >  AP012306/3395808‑3396101

atggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAActct                                                                                                                                                  >  1:3654557/1‑150 (MQ=255)

 tggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTa                                                                                                                                                 >  1:1285369/1‑150 (MQ=255)

  ggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTAt                                                                                                                                                >  1:2914849/1‑150 (MQ=255)

   gatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTAtt                                                                                                                                               >  2:1334633/1‑150 (MQ=255)

    atTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTAttt                                                                                                                                              >  1:2314770/1‑150 (MQ=255)

    atTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTAttt                                                                                                                                              >  1:3746740/1‑150 (MQ=255)

     tttGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTAtttt                                                                                                                                             >  2:2572617/1‑150 (MQ=255)

     tttGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTAtttt                                                                                                                                             >  1:2241531/1‑150 (MQ=255)

           ttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCgctg                                                                                                                                       >  2:1775949/1‑150 (MQ=255)

             cctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCgctgct                                                                                                                                     >  1:3359838/1‑150 (MQ=255)

              ctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGctgctg                                                                                                                                    >  1:2264465/1‑150 (MQ=255)

                  gTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAg                                                                                                                                >  2:1893641/1‑150 (MQ=255)

                       gCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAAc                                                                                                                           >  1:1922915/1‑150 (MQ=255)

                         aGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGa                                                                                                                         >  1:153664/1‑150 (MQ=255)

                           aCAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAg                                                                                                                       >  2:1376176/1‑150 (MQ=255)

                           aCAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAg                                                                                                                       >  2:3108317/1‑150 (MQ=255)

                            cAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGt                                                                                                                      >  1:3536505/1‑150 (MQ=255)

                            cAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGt                                                                                                                      >  1:2187049/1‑150 (MQ=255)

                               cTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTaa                                                                                                                   >  2:1287169/1‑150 (MQ=255)

                               cTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCCAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTaa                                                                                                                   >  2:634072/1‑150 (MQ=255)

                                   tACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTc                                                                                                               >  1:2223043/1‑150 (MQ=255)

                                          ccGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAAc                                                                                                        >  2:3602119/1‑150 (MQ=255)

                                           cGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAAcc                                                                                                       >  2:1618257/1‑150 (MQ=255)

                                               ccTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTGT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaa                                                                                                   >  1:4112139/1‑150 (MQ=255)

                                                   atgaCATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACg                                                                                               >  1:2134219/1‑150 (MQ=255)

                                                   atgaCATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACg                                                                                               >  1:2972529/1‑150 (MQ=255)

                                                     gaCATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGcc                                                                                             >  1:3722476/1‑150 (MQ=255)

                                                     gaCATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGcc                                                                                             >  2:76024/1‑150 (MQ=255)

                                                         tCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGca                                                                                         >  2:550301/1‑150 (MQ=255)

                                                         atcgccgttCCCCCAACC‑AAACC‑CCGCCGTTT‑CCCCCCCGCCGCTGGTAATCCTATCTCGGAAAAAATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGc                                                                                          <  2:3462350/143‑1 (MQ=255)

                                                           tACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaac                                                                                       >  2:317304/1‑150 (MQ=255)

                                                            tcctttCCCCTAACCAAAA‑C‑CCGCCGTTT‑CACCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaac                                                                                       <  1:4199167/147‑1 (MQ=255)

                                                            aCGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaaca                                                                                      >  1:2656430/1‑150 (MQ=255)

                                                            aCGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaaca                                                                                      >  1:4416345/1‑150 (MQ=255)

                                                              gTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACaaa                                                                                    >  2:1084588/1‑150 (MQ=255)

                                                               tttCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACaaaa                                                                                   >  1:115956/1‑150 (MQ=255)

                                                               attCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTCGTGATACCATCTCTGGGAAGAGTCACCCGCCGAAAGAAGGTAAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGACCGCAACaaaa                                                                                   >  2:1989474/2‑150 (MQ=255)

                                                                ttCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAAt                                                                                  >  2:3714425/1‑150 (MQ=255)

                                                                 tCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATc                                                                                 >  2:2386124/1‑150 (MQ=255)

                                                                 cccccAAACC‑AAA‑C‑CCCCCCTTTCCACCCCCCGCACTGGTGATACCATCTCGGGAAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAAt                                                                                  <  2:65693/149‑1 (MQ=255)

                                                                      tAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCtt                                                                            >  1:4076535/1‑150 (MQ=255)

                                                                       aagc‑AAA‑TCCCGCCGTTT‑CAACCCCCGCCCTGGTGATACCATCTCTGGAAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCtt                                                                            <  1:1436255/147‑1 (MQ=255)

                                                                       aGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCttt                                                                           >  1:3284696/1‑150 (MQ=255)

                                                                          c‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgag                                                                        >  2:2318909/1‑150 (MQ=255)

                                                                            aaa‑C‑CCGCCGTTC‑CACCCCCCGCCCTGGTAATCCCATCTCTGGTAAAATCCCCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgaga                                                                       <  2:564630/150‑1 (MQ=255)

                                                                             aaaccccgccgTTT‑CACCCTCCGCACTGGTGATCCCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgag                                                                        <  1:2341635/145‑1 (MQ=255)

                                                                                   cgcctTTC‑CACCCCCCGCCCTGGTAATACCATTTTTGGAAAGTTTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTa                                                                  <  2:3310823/150‑1 (MQ=255)

                                                                                      cgTTT‑CACCCCCCGCCCTGGTTATCCCTTCTTTGGAAAGATTCCCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAcc                                                               <  2:794518/150‑1 (MQ=255)

                                                                                      cgTTT‑CACCCCCCGCACTGGTGATACCATCTCTGGAAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAcc                                                               <  2:3996917/150‑1 (MQ=255)

                                                                                       tttt‑CACCCCCCGCTCTGGTCATCCCATCTCTGGTAAGCTTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAccc                                                              <  2:1434964/149‑1 (MQ=255)

                                                                                        ttt‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAcccc                                                             >  2:4123213/1‑150 (MQ=255)

                                                                                            cAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCt                                                          >  2:1209878/1‑150 (MQ=255)

                                                                                             accccccGCACTGGTAACACCATCTCTGAAAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTg                                                         <  2:3286731/145‑1 (MQ=255)

                                                                                              aCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGc                                                        >  1:3994808/1‑150 (MQ=255)

                                                                                              aCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGc                                                        >  2:2596955/1‑150 (MQ=255)

                                                                                                cTCCGCATTGGTGATCCCATCTCTGGTAAGATCCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCAc                                                      <  1:1148050/150‑1 (MQ=255)

                                                                                                  ccGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGc                                                    >  1:868848/1‑150 (MQ=255)

                                                                                                   cGCACTGGTGATACCATCTCTGGTAAGTTTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCa                                                   <  1:3147740/150‑1 (MQ=255)

                                                                                                   cGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCa                                                   >  1:1049906/1‑150 (MQ=255)

                                                                                                       cTGGTGATACCATCTCTGGTAAGATCCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAct                                               <  2:1525699/150‑1 (MQ=255)

                                                                                                        tGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAActc                                              >  2:2490367/1‑150 (MQ=255)

                                                                                                              tACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCt                                        >  2:253836/1‑150 (MQ=255)

                                                                                                               aCCATCTCTGGTAAGATCCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTg                                       <  1:120964/150‑1 (MQ=255)

                                                                                                                  aTCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGt                                    >  2:4440720/1‑150 (MQ=255)

                                                                                                                   tctcTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTa                                   >  1:2078711/1‑150 (MQ=255)

                                                                                                                   tctcTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCATTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTa                                   <  2:2196259/150‑1 (MQ=255)

                                                                                                                   cccctGGAAAGATGCCACCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAATCCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTTGCTTCTGCGTa                                   <  2:3671573/147‑1 (MQ=255)

                                                                                                                      ctGGTAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATgg                                >  2:2102445/1‑150 (MQ=255)

                                                                                                                      ctGGAAAGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTTGTCTGCGTATgg                                <  2:4345403/150‑1 (MQ=255)

                                                                                                                        ggaaaGATCCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGaa                              <  2:4304584/147‑1 (MQ=255)

                                                                                                                           aaGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGt                           >  2:1737205/1‑150 (MQ=255)

                                                                                                                            aGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTg                          <  1:431714/150‑1 (MQ=255)

                                                                                                                            aGATTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTg                          <  1:2947806/150‑1 (MQ=255)

                                                                                                                             gttccccccgccGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTgg                         <  1:3227872/144‑1 (MQ=255)

                                                                                                                             gATCCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTgg                         <  1:2420681/150‑1 (MQ=255)

                                                                                                                              atccgcccgccGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGt                        <  2:280160/147‑1 (MQ=255)

                                                                                                                              aTTCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGt                        <  1:1103589/150‑1 (MQ=255)

                                                                                                                              aTTCCCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGt                        <  2:2830834/150‑1 (MQ=255)

                                                                                                                               ttCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTa                       >  1:713408/1‑150 (MQ=255)

                                                                                                                                tCGCCCGCCGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTaa                      >  2:1672869/1‑150 (MQ=255)

                                                                                                                                 cgcccgccGAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAAc                     <  1:1776675/150‑1 (MQ=255)

                                                                                                                                    ccgccgAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGt                  <  1:271116/150‑1 (MQ=255)

                                                                                                                                     cgccgAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGtt                 <  2:3034356/150‑1 (MQ=255)

                                                                                                                                     cgccgAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGtt                 >  1:2127549/1‑150 (MQ=255)

                                                                                                                                      gccgAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTc                >  2:2973257/1‑150 (MQ=255)

                                                                                                                                       ccgAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTTACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCt               <  2:3922539/150‑1 (MQ=255)

                                                                                                                                         gAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTAc             >  2:3452368/1‑150 (MQ=255)

                                                                                                                                         gAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTAc             >  1:394060/1‑150 (MQ=255)

                                                                                                                                         gAAAGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTAc             <  1:1408520/150‑1 (MQ=255)

                                                                                                                                          aaaGAAGGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACt            <  2:2932699/150‑1 (MQ=255)

                                                                                                                                           aagaagGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTg           <  1:2441647/150‑1 (MQ=255)

                                                                                                                                           aagaagGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTg           >  1:573494/1‑150 (MQ=255)

                                                                                                                                            agaagGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGa          >  2:3793877/1‑150 (MQ=255)

                                                                                                                                             gaagGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGaa         <  1:1704266/150‑1 (MQ=255)

                                                                                                                                               agGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGa       >  1:3011972/1‑150 (MQ=255)

                                                                                                                                               agGTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGa       <  2:1234944/150‑1 (MQ=255)

                                                                                                                                                ggTGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGAt      >  2:688284/1‑150 (MQ=255)

                                                                                                                                                  tGAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGAttt    <  1:201700/150‑1 (MQ=255)

                                                                                                                                                   gAACTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTa   >  1:2836400/1‑150 (MQ=255)

                                                                                                                                                    aaCTCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAc                                                <  1:997844/104‑1 (MQ=255)

ATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAA‑T‑CCGCCGTTT‑CAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAA  >  AP012306/3395808‑3396101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`