Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,128,4120TG22.9% 802.5 / 37.0 306V29G (GTG→GGG) yciOconserved protein
Reads supporting (aligned to +/- strand):  ref base T (42/194);  new base G (70/0);  total (112/194)
Fisher's exact test for biased strand distribution p-value = 7.39e-40
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 355 total aligned reads displayed.

CCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTATT  >  AP012306/1128263‑1128561
                                                                                                                                                     |                                                                                                                                                      
    aGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGAtt                                                                                                                                                    <  1:1149661/150‑1 (MQ=255)
        gTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGttt                                                                                                                                                <  2:3489464/150‑1 (MQ=255)
          tGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGgttta                                                                                                                                              >  2:4130550/1‑148 (MQ=255)
           gAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATc                                                                                                                                             <  1:390451/150‑1 (MQ=255)
                cGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACt                                                                                                                                        >  2:3375874/1‑150 (MQ=255)
                 gTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTg                                                                                                                                       >  1:3045097/1‑150 (MQ=255)
                    tGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTTTACAACTGAtt                                                                                                                                    >  2:3133416/1‑150 (MQ=255)
                      gCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTcc                                                                                                                                  <  2:3616443/150‑1 (MQ=255)
                      gCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTcc                                                                                                                                  <  1:3192893/150‑1 (MQ=255)
                               ggAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTTTCCAACTGGTTGCGGCTATtcg                                                                                                                         >  2:1965423/1‑150 (MQ=255)
                                      ccGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCt                                                                                                                  <  1:4223366/150‑1 (MQ=255)
                                      ccGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTATCCCACTGATTTCCGCTATGCGCTCGGCt                                                                                                                  >  1:1011280/1‑150 (MQ=255)
                                       cGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTTATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑g                                                                                                                <  2:2922812/150‑1 (MQ=255)
                                        gCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑Gt                                                                                                               <  1:632006/150‑1 (MQ=255)
                                          aGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTTCGGCTATGCGCCCGGCT‑Gtta                                                                                                             >  2:3843663/1‑148 (MQ=255)
                                            aaCACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTaaaa                                                                                                           <  2:4011986/150‑1 (MQ=255)
                                             acacCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAAt                                                                                                          >  1:920887/1‑150 (MQ=255)
                                                  acaGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAg                                                                                                     <  1:308495/150‑1 (MQ=255)
                                                  acaGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAg                                                                                                     >  1:3440776/1‑150 (MQ=255)
                                                  acaGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAg                                                                                                     >  1:2558918/1‑150 (MQ=255)
                                                   caGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGa                                                                                                    <  1:3016798/150‑1 (MQ=255)
                                                   caGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGa                                                                                                    <  1:3941707/150‑1 (MQ=255)
                                                   caGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTATTCAACTGATTTCGGCCATGCGCTCGGCT‑GAAAAATTTaaag                                                                                                    >  2:3069724/1‑148 (MQ=255)
                                                    agagagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGAt                                                                                                   <  1:750317/150‑1 (MQ=255)
                                                    agagagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGAt                                                                                                   <  2:1404831/150‑1 (MQ=255)
                                                    agagagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGAt                                                                                                   >  1:179292/1‑150 (MQ=255)
                                                     gcgagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGGTCGTGCGTTAAGGCGGGGGGTAGGTTTATACAACTGGTTTCGGAATTGCGCTCAGCC‑CTTAGATTGAAGATa                                                                                                  >  2:905201/3‑150 (MQ=255)
                                                      agagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATaa                                                                                                 <  1:2858841/150‑1 (MQ=255)
                                                      agagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATaa                                                                                                 <  1:4069927/150‑1 (MQ=255)
                                                      agagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATaa                                                                                                 <  1:1188351/150‑1 (MQ=255)
                                                      agagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATaa                                                                                                 <  1:197002/150‑1 (MQ=255)
                                                      agagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATaa                                                                                                 >  1:2661769/1‑150 (MQ=255)
                                                       gagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATaaa                                                                                                <  2:740851/150‑1 (MQ=255)
                                                       gagGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATaaa                                                                                                <  2:4413861/150‑1 (MQ=255)
                                                        agGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCCACTGATTCCGGCTATGCGCCCGGCT‑GTAAAATTTAAGATaaaa                                                                                               >  2:3295773/1‑150 (MQ=255)
                                                          ggAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGGTTGTTTATTCCACCGGTTCCCGCTATGCGCTCCGGT‑GTAAAAATTAAGATaaaaaa                                                                                             >  2:2781634/1‑149 (MQ=255)
                                                            aaTTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAAcgc                                                                                           <  2:3773924/150‑1 (MQ=255)
                                                            aaTTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTTAAGATAAAAAcgc                                                                                           >  2:3722489/1‑150 (MQ=255)
                                                            aaTTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAAcgc                                                                                           >  1:2519585/1‑150 (MQ=255)
                                                              ttATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGa                                                                                         <  2:1248981/150‑1 (MQ=255)
                                                              ttATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTTTCCAACTGATTCCGGGTATTCGCTCGGGC‑GTAAAATTGAAGATAAAAAAGCGa                                                                                         >  2:3956294/1‑150 (MQ=255)
                                                                aTGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATg                                                                                       >  1:3576173/1‑150 (MQ=255)
                                                                aTGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATg                                                                                       >  1:2316173/1‑150 (MQ=255)
                                                                 tGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATgg                                                                                      <  1:3059738/150‑1 (MQ=255)
                                                                 tGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATgg                                                                                      <  2:2121401/150‑1 (MQ=255)
                                                                  gAGCCAGTTTTTTTATATTCAGCCTGATACCCCAGGGGAAGTTCTGATCAACCAGGCGGTGGGGGTCGGGCGTAAGGGGGGGGGGATTGTTTATCAAACTGATTCGGGGTAGGGGCTGGGCT‑GAAAAATTGAAGAAAAAAACGCGATGGa                                                                                     <  2:1202037/150‑1 (MQ=255)
                                                                   aGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAg                                                                                    <  2:1115536/150‑1 (MQ=255)
                                                                   aGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTTAAGATAAAAACGCGATGGAg                                                                                    >  1:2893477/1‑150 (MQ=255)
                                                                    gCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGc                                                                                   <  2:808085/150‑1 (MQ=255)
                                                                      cAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGt                                                                                 <  1:2190263/150‑1 (MQ=255)
                                                                      cAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGAATGTTTAACCAACTGATTACCGGTCTGCGCCACGCTGGTAAAATT‑AAGATAAAAAAGCGATGGAGCGt                                                                                 >  2:616391/1‑150 (MQ=255)
                                                                       aGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTTAAGATAAAAACGCGATGGAGCGTa                                                                                >  2:1626605/1‑150 (MQ=255)
                                                                          ttttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTAttt                                                                             <  2:4309833/150‑1 (MQ=255)
                                                                            ttttATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGt                                                                           <  1:148300/150‑1 (MQ=255)
                                                                               tataTTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTGAAATTGAAGATAAAAAATCGATTGGGCGTAtttttagt                                                                        >  2:2273630/1‑145 (MQ=255)
                                                                                 tatTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGGATTTGTCGTAt                                                                      >  1:3341545/1‑150 (MQ=255)
                                                                                   ttCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTc                                                                    <  1:450962/150‑1 (MQ=255)
                                                                                   ttCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTc                                                                    >  1:2250834/1‑150 (MQ=255)
                                                                                      aTCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGcc                                                                 <  1:4109313/150‑1 (MQ=255)
                                                                                       tCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTNGCCa                                                                <  1:2781585/150‑1 (MQ=255)
                                                                                       tCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTTATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGGGCGGATTTTTCGTATTCGCCa                                                                >  2:2710287/1‑150 (MQ=255)
                                                                                       tCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGGATTTGTCGTATTCGCCa                                                                >  2:4044727/1‑150 (MQ=255)
                                                                                        ccTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAg                                                               <  1:3263299/150‑1 (MQ=255)
                                                                                         cTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGc                                                              >  1:168252/1‑150 (MQ=255)
                                                                                          tGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCt                                                             <  2:3255361/150‑1 (MQ=255)
                                                                                          tGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCt                                                             <  1:2395616/150‑1 (MQ=255)
                                                                                          tGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCt                                                             >  2:517809/1‑150 (MQ=255)
                                                                                              aaCCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCg                                                         <  2:3014991/150‑1 (MQ=255)
                                                                                                   acaGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACgg                                                    <  1:1082340/150‑1 (MQ=255)
                                                                                                     aGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTc                                                  <  1:1320795/150‑1 (MQ=255)
                                                                                                     aGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGGGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTc                                                  >  2:2406109/1‑150 (MQ=255)
                                                                                                     aGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTc                                                  >  1:671670/1‑150 (MQ=255)
                                                                                                     aGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGAAgggc                                                  >  2:468099/1‑148 (MQ=255)
                                                                                                         aCGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACaa                                              <  2:2451942/150‑1 (MQ=255)
                                                                                                                aTCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTAcc                                       <  1:2072971/150‑1 (MQ=255)
                                                                                                                aTCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTAcc                                       <  2:153689/150‑1 (MQ=255)
                                                                                                                   aaCCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTg                                    <  1:3801289/150‑1 (MQ=255)
                                                                                                                    aCCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGa                                   >  1:3986251/1‑150 (MQ=255)
                                                                                                                          cGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTc                             <  1:2924959/150‑1 (MQ=255)
                                                                                                                           ggTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTcg                            >  1:1563919/1‑150 (MQ=255)
                                                                                                                             tGGAGATCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTcgcg                          >  2:2073255/1‑150 (MQ=255)
                                                                                                                               gagaTCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGTTCACAACTTTACCCTGATGTGTCGCGAt                        <  2:1275962/150‑1 (MQ=255)
                                                                                                                                agaTCGTGCGTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGGATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACCTTACCCTGATGTGTCGCGATc                       >  1:956700/1‑150 (MQ=255)
                                                                                                                                  aTCGTGCGTATAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCtt                     <  1:4294342/150‑1 (MQ=255)
                                                                                                                                  aTCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCCCCGCT‑GTAAAATTGAAGATAAGAACCCGATGGAGCGTCTTTTTCCTCTTCGCCAGCTCCCGGCCGGTCACCACTTTACCCTGATGTGTCGCGCTCtt                     >  2:3555246/1‑150 (MQ=255)
                                                                                                                                     gTGCGTAAAGGCGGGGTGATTGTTTTTCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCt                  <  2:2317575/150‑1 (MQ=255)
                                                                                                                                       gCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGa                <  2:1350012/150‑1 (MQ=255)
                                                                                                                                        cGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGaa               <  2:1452563/150‑1 (MQ=255)
                                                                                                                                         gTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAAc              <  1:3734893/150‑1 (MQ=255)
                                                                                                                                         gTAAAGGCGGGGGGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGGGCGGATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAAc              >  2:86454/1‑150 (MQ=255)
                                                                                                                                          tAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAAct             <  1:4375089/150‑1 (MQ=255)
                                                                                                                                           aaaGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAActc            <  2:1835201/150‑1 (MQ=255)
                                                                                                                                            aaGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAActct           >  1:3663874/1‑150 (MQ=255)
                                                                                                                                            aaGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAActct           >  2:1680887/1‑150 (MQ=255)
                                                                                                                                            aaGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAActct           <  2:2488557/150‑1 (MQ=255)
                                                                                                                                             aGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACtctc          <  1:2109818/150‑1 (MQ=255)
                                                                                                                                              ggCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCg         <  2:1871764/150‑1 (MQ=255)
                                                                                                                                                cGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGAc       <  1:1846744/150‑1 (MQ=255)
                                                                                                                                                cGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCATATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGAc       <  2:3339769/150‑1 (MQ=255)
                                                                                                                                                    gTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTAt   <  1:3458561/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CCGCAGGCGTTGAAGGCGTCTGGCAGCTATGGGAACAGCCGCAGAACACCACAGAGAGGGAATTATGAGCCAGTTTTTTTATATTCATCCTGATAACCCACAGCAACGTCTGATCAACCAGGCGGTGGAGATCGTGCGTAAAGGCGGGGTGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCT‑GTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTATT  >  AP012306/1128263‑1128561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑