Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,944,2880AC38.6% 404.6 / 54.0 228G361G (GGT→GGG) menEo‑succinylbenzoate‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (101/39);  new base C (0/88);  total (101/127)
Fisher's exact test for biased strand distribution p-value = 1.45e-32
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 297 total aligned reads displayed.

ACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCTGCCAT  >  AP012306/1944139‑1944437
                                                                                                                                                     |                                                                                                                                                     
aCCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATa                                                                                                                                                       >  2:3869624/1‑150 (MQ=255)
 cccATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATAc                                                                                                                                                      >  1:1211817/1‑150 (MQ=255)
   cATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATAccc                                                                                                                                                    <  1:3361145/150‑1 (MQ=255)
    aTTTACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCct                                                                                                                                                   <  1:1369132/150‑1 (MQ=255)
    aTTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCct                                                                                                                                                   >  2:3399508/1‑150 (MQ=255)
     ttCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCctc                                                                                                                                                  <  1:3531621/150‑1 (MQ=255)
     ttCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCctc                                                                                                                                                  <  2:1561107/150‑1 (MQ=255)
      tCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCctct                                                                                                                                                 <  2:4182648/150‑1 (MQ=255)
       cACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGCATACCCtctc                                                                                                                                                >  1:4360352/1‑150 (MQ=255)
       cACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCtctc                                                                                                                                                <  1:2088076/150‑1 (MQ=255)
        aCTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTcc                                                                                                                                               <  1:4039584/150‑1 (MQ=255)
        aCTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTcc                                                                                                                                               <  2:522847/150‑1 (MQ=255)
        aCTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTcc                                                                                                                                               <  2:654182/150‑1 (MQ=255)
          tAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGc                                                                                                                                             >  2:3211488/1‑150 (MQ=255)
           aaGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGcc                                                                                                                                            >  1:3237853/1‑150 (MQ=255)
           aaGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGcc                                                                                                                                            >  1:4187196/1‑150 (MQ=255)
               tCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTg                                                                                                                                        <  1:237648/150‑1 (MQ=255)
                cAACGCTTCCGTGGTCATACTCCACCCCCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTga                                                                                                                                       <  1:3694937/150‑1 (MQ=255)
                cAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTga                                                                                                                                       >  1:986828/1‑150 (MQ=255)
                   cGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTgaaga                                                                                                                                    >  1:875715/1‑150 (MQ=255)
                     ctctCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAAt                                                                                                                                  >  1:2477228/1‑150 (MQ=255)
                      tctcTTGGTCATACCCCATCCCCGCCACCGGCCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAACCACCTGAAAAACCGCAGGATGTGCAGCAATTCCGCGCGCGATTCCTCCCGGCTAAATCCCCTCTCCGCCACTGAAGAATa                                                                                                                                 <  2:915771/150‑1 (MQ=255)
                      tctcGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATa                                                                                                                                 >  1:2585006/1‑150 (MQ=255)
                        tcGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGa                                                                                                                               <  1:827749/150‑1 (MQ=255)
                        tcGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGa                                                                                                                               <  1:457178/150‑1 (MQ=255)
                          gTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGAtt                                                                                                                             >  1:1430609/1‑150 (MQ=255)
                           tGGTCATACTCCATCACAGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTg                                                                                                                            >  2:4245247/1‑150 (MQ=255)
                            ggTCATACTCCATCCCCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGt                                                                                                                           <  1:4259545/150‑1 (MQ=255)
                             gTCATACTCCATCACCGCCACCGGTCGATGACCAAGCTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGAAATTACGCGCTCGACTTTTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTc                                                                                                                          <  1:1132827/150‑1 (MQ=255)
                             gTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTc                                                                                                                          <  1:2255671/150‑1 (MQ=255)
                              tCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACCATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCt                                                                                                                         >  2:4025882/1‑150 (MQ=255)
                               attccTCCATCACCCCCACGGGTCGATGACAAATCCCCTTGCCGGCAACGGGGACAATAAACACCTGCAAACCCGCAGGAGGTGCAGAAATTACGCCCTCGCCTTCTCCCGGCGGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTa                                                                                                                        <  2:4387977/146‑1 (MQ=255)
                                 tccTCCATCCCCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAACCCGCAGGATGTGCAGCAATTACGCGCTCGCCTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTaaa                                                                                                                      <  2:609757/148‑1 (MQ=255)
                                  aCTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAAc                                                                                                                     >  2:2970554/1‑150 (MQ=255)
                                    tCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCCGGATGTGCAGCCATTACGCGCTCGCCTTCTCCCGGCTGAACACCCTCTCCGCCACTGAAGAGTAGCTTGTCTAAACgg                                                                                                                   >  2:3821883/1‑149 (MQ=255)
                                     ccATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTc                                                                                                                  >  1:2047014/1‑150 (MQ=255)
                                       aTCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGACTAGATTGTCTAAACGTCCg                                                                                                                >  2:2843688/1‑150 (MQ=255)
                                         cACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAACCCGCAGGATGTGCAGCAATTCCGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGAc                                                                                                              <  1:1834942/150‑1 (MQ=255)
                                          cccGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACa                                                                                                             <  2:3758968/149‑1 (MQ=255)
                                          aCCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACa                                                                                                             <  1:1726276/150‑1 (MQ=255)
                                          aCCCCCCCCGGTCGATGACAAACCTCCTTGTCGGCAAGGGGGACGATAACCACCTCAAAAGCCGCAGGATGTGCACCAATTACGCGCTCGATTTCTCCCGGCGGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACa                                                                                                             <  2:445005/150‑1 (MQ=255)
                                            cGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAAt                                                                                                           <  1:3817749/150‑1 (MQ=255)
                                             ccccccGCTCGATACCCAAACCCCTTCCCGGCAACGGGGCCGATAACCCCCTCCAAACCCCCAGCATCTCCACCAATTCCGCCCTCCCCTTCTCCCGCCTGAATCCCCCCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATg                                                                                                          <  2:2900797/146‑1 (MQ=255)
                                              ccACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCGGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATgg                                                                                                         <  1:1681848/150‑1 (MQ=255)
                                                 ccGGTCGATGACCAAACCCCTTGTCGGCAACGGGGACGATAAACCCCTCCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTCCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCa                                                                                                      <  2:1505913/150‑1 (MQ=255)
                                                   ggTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGc                                                                                                    <  2:2147567/150‑1 (MQ=255)
                                                   ggTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGc                                                                                                    <  1:215325/150‑1 (MQ=255)
                                                    gTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCt                                                                                                   >  2:2750710/1‑150 (MQ=255)
                                                    gTCGAGGACAAAACTCTTGGTCGGCACCGGGGACGAAAAACCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCt                                                                                                   <  2:1025772/150‑1 (MQ=255)
                                                     tCGATGACCAACCTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAACCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCtt                                                                                                  <  2:2372458/150‑1 (MQ=255)
                                                       gATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGc                                                                                                >  1:3095231/1‑150 (MQ=255)
                                                         tGACCAACCTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGCCCGACAATGGTCAGCTTGCCa                                                                                              <  2:2532398/150‑1 (MQ=255)
                                                           aCCAACCCCCTCGTCGCCAAGGGGAACGATAAACCCCGGCAAACCGGCAGTATGGCCAGCAATACCGCGCTGGACTTCTTCCGCCTGAATCCCTTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCAtt                                                                                            <  2:3239657/150‑1 (MQ=255)
                                                           aCCAAACTCCTTGTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCAtt                                                                                            <  2:3920115/150‑1 (MQ=255)
                                                            ccAAACCCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCAACTTCTTCCGGCTGAACACCCTCCCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTa                                                                                           <  2:4103263/150‑1 (MQ=255)
                                                             cAACCTCCTTGTCGGCACCGGGGACGAAAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACCCGCCCGACTTCTGCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTAt                                                                                          <  2:3082540/150‑1 (MQ=255)
                                                             cAACCTCCTTGCCGGCAACGGGGACGAAAACCCCCTGAAAAACCGCAGGTTGCGCAGCAATTACGCGTTCACTTTCTTCCGCTTAAATCCCCTCCCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTAt                                                                                          <  2:3968986/150‑1 (MQ=255)
                                                              aaccTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATg                                                                                         <  1:791677/147‑1 (MQ=255)
                                                                aCTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCa                                                                                       >  2:2933596/1‑150 (MQ=255)
                                                                aCTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCa                                                                                       >  1:41039/1‑150 (MQ=255)
                                                                 cTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGATTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCAt                                                                                      <  1:2763070/150‑1 (MQ=255)
                                                                          ggCAACGGGGACGATAAACCCCTGCAAACCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACcgcg                                                                             <  2:1915734/150‑1 (MQ=255)
                                                                              aCGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATcg                                                                         >  2:1149241/1‑150 (MQ=255)
                                                                                ggggACGATAAACACCTGCAAAACCGCAGGTTGTGCAGCAATTACGCGCTCGCCTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATcgcg                                                                       <  1:3434151/150‑1 (MQ=255)
                                                                                        tAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgtagcgt                                                               >  1:3485360/1‑150 (MQ=255)
                                                                                         aaaCACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgtagcgta                                                              >  1:616741/1‑150 (MQ=255)
                                                                                          aaCACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTAc                                                             >  2:137948/1‑150 (MQ=255)
                                                                                           accccTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTAcc                                                            <  1:3667613/147‑1 (MQ=255)
                                                                                           acacCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTAcc                                                            >  2:2705442/1‑150 (MQ=255)
                                                                                            cacCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCa                                                           >  2:261542/1‑150 (MQ=255)
                                                                                             cccTGCAAACCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAg                                                          <  2:2806402/149‑1 (MQ=255)
                                                                                             acCTGCAAACCCGCAGGATGGGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAg                                                          <  1:3572980/150‑1 (MQ=255)
                                                                                               cTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGcc                                                        >  2:206692/1‑150 (MQ=255)
                                                                                                tGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCt                                                       <  1:2991370/150‑1 (MQ=255)
                                                                                                 gCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCGGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCtt                                                      <  1:728347/150‑1 (MQ=255)
                                                                                                  cAAACCCGCAGGATGTGAAGCAATTACGCGCTCGCCTTCTCCCGGCTGAATCCCCCCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCTACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTc                                                     <  2:16377/150‑1 (MQ=255)
                                                                                                  cAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTc                                                     >  2:2194899/1‑150 (MQ=255)
                                                                                                    aaaCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGt                                                   >  1:2806823/1‑150 (MQ=255)
                                                                                                     acccGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCAGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTc                                                  <  1:1970385/148‑1 (MQ=255)
                                                                                                         gCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTa                                              >  2:2223491/1‑150 (MQ=255)
                                                                                                          cAGGATGTGCAGCAATTACGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTaa                                             <  2:2635372/150‑1 (MQ=255)
                                                                                                            ggATGTGCGCCAATTACGGCCTCGTCTTCCCCCGCCTAAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAAcc                                           <  2:3498217/150‑1 (MQ=255)
                                                                                                              aTGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAg                                         >  2:1195977/1‑150 (MQ=255)
                                                                                                                gtgCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTg                                       <  1:2217489/150‑1 (MQ=255)
                                                                                                                gtgCAGCAATTACGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTg                                       <  1:2922868/150‑1 (MQ=255)
                                                                                                                gggcagcaATTACGGGCTCGACTTCTTCCGGCTGAATCCCCTCCCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTg                                       <  2:2479962/148‑1 (MQ=255)
                                                                                                                 tgCAGCAATTACGCGCTCGTCTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGa                                      <  2:2649697/150‑1 (MQ=255)
                                                                                                                   cagcaATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGaaa                                    >  1:2635308/1‑150 (MQ=255)
                                                                                                                   cagcaATTACGCGCTCGACTTCTCCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGaaa                                    <  1:1013934/150‑1 (MQ=255)
                                                                                                                      caATTACGCGCTCGTCTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCa                                 <  2:1985766/150‑1 (MQ=255)
                                                                                                                      caATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCa                                 <  2:4062001/150‑1 (MQ=255)
                                                                                                                       aaTTACGCGCTCGACTTCTCCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAg                                <  2:851441/150‑1 (MQ=255)
                                                                                                                       aaTTACACGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAg                                >  1:4390595/1‑150 (MQ=255)
                                                                                                                          tACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTg                             <  2:664503/150‑1 (MQ=255)
                                                                                                                                    aCTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGc                   >  2:785520/1‑150 (MQ=255)
                                                                                                                                    aCTTCTCCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCGCCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGc                   <  1:637555/150‑1 (MQ=255)
                                                                                                                                       tcttcCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAg                <  1:210430/150‑1 (MQ=255)
                                                                                                                                            cGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAAcc           >  2:3569732/1‑150 (MQ=255)
                                                                                                                                               cTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTc        <  2:183698/150‑1 (MQ=255)
                                                                                                                                                 gAATCCCCTTCCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCTg      <  2:2681384/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCTGCCAT  >  AP012306/1944139‑1944437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑