Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,758,9090TG24.3% 585.8 / 20.1 230G33G (GGT→GGG) agaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
Reads supporting (aligned to +/- strand):  ref base T (42/132);  new base G (56/0);  total (98/132)
Fisher's exact test for biased strand distribution p-value = 5.56e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 304 total aligned reads displayed.

AAGCGCAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGA  >  AP012306/2758760‑2759058
                                                                                                                                                      |                                                                                                                                                      
  gcgcAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGttt                                                                                                                                                      >  2:3379343/1‑150 (MQ=255)
   cgcAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGtttt                                                                                                                                                     <  1:898653/150‑1 (MQ=255)
    gcAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGTC‑CGGTTTa                                                                                                                                                    >  2:471805/1‑150 (MQ=255)
     cAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTAc                                                                                                                                                   <  2:1556680/150‑1 (MQ=255)
       aCCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCt                                                                                                                                                 <  1:1584653/150‑1 (MQ=255)
        ccGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTg                                                                                                                                                <  2:1960628/150‑1 (MQ=255)
        ccGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTg                                                                                                                                                <  1:3607110/150‑1 (MQ=255)
            aGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCg                                                                                                                                            <  1:3829907/150‑1 (MQ=255)
             gCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGa                                                                                                                                           >  2:3055297/1‑150 (MQ=255)
                cGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGt                                                                                                                                        >  1:3380893/1‑150 (MQ=255)
                 gTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGtt                                                                                                                                       <  2:2977535/150‑1 (MQ=255)
                   tGCCAGTAAAAATGAAGAAGAGGATTACAGCAATGGGATCTGAAACCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGGGATGtggg                                                                                                                                     >  2:620562/1‑147 (MQ=255)
                   tGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCTAtttttc                                                                                                                                     >  2:3126191/1‑148 (MQ=255)
                    gCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCCGGGCGATGTTGcc                                                                                                                                    >  1:631566/1‑150 (MQ=255)
                    gCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGCC‑GGATTTTCCTGGGCGATGTTGcc                                                                                                                                    >  1:1554553/1‑150 (MQ=255)
                     ccAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTTCTGGGCGATGTTGGC‑g                                                                                                                                  >  1:2667410/1‑150 (MQ=255)
                      cAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTTCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTTCC‑gg                                                                                                                                 >  1:1969650/1‑149 (MQ=255)
                       aGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGTCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGCC‑CGTTTTACCTTCGGGATGTTGCC‑GAt                                                                                                                                >  1:244993/1‑150 (MQ=255)
                           aaaaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTg                                                                                                                            <  1:4001604/150‑1 (MQ=255)
                            aaaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGa                                                                                                                           <  1:1869398/150‑1 (MQ=255)
                            aaaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTACTGGGCGATGTTGCC‑GATCCCGa                                                                                                                           >  1:709789/1‑150 (MQ=255)
                             aaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGaa                                                                                                                          <  2:4120336/150‑1 (MQ=255)
                             aaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGaa                                                                                                                          >  1:2163188/1‑150 (MQ=255)
                             aaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGGGATTGCA‑GGTCCTGag                                                                                                                          >  2:248415/1‑149 (MQ=255)
                               tGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGgaaa                                                                                                                        >  1:1094473/1‑150 (MQ=255)
                                  agaagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTTGGCGATGTTTCC‑GATCCTGAAAAAGa                                                                                                                     >  2:2383667/1‑150 (MQ=255)
                                  agaagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGa                                                                                                                     >  1:2322545/1‑150 (MQ=255)
                                  agaagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGGTGGTGCC‑GATCCTGAAAAAGa                                                                                                                     >  2:1622090/1‑150 (MQ=255)
                                   gaagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGAt                                                                                                                    <  2:1825594/150‑1 (MQ=255)
                                    aagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGAtt                                                                                                                   >  1:1637563/1‑150 (MQ=255)
                                     agagGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGCGTTTGCC‑GGTCCTGAAAAAAAttt                                                                                                                  >  2:234190/1‑150 (MQ=255)
                                     agagGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTTGGCGATGTTTCC‑GATCCTGAAAAAAAttt                                                                                                                  >  2:438347/1‑150 (MQ=255)
                                       agGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGCATTTGCCGGACCCGGAAAAAAAtttt                                                                                                                 >  2:1493086/1‑149 (MQ=255)
                                        ggACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTtata                                                                                                               <  2:553054/150‑1 (MQ=255)
                                            tACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTTTTTGGGGGAGTTTGCC‑GTACCCGAAAAAAATTTTTAAga                                                                                                            >  2:1067448/1‑149 (MQ=255)
                                            tACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGga                                                                                                           <  1:2289846/150‑1 (MQ=255)
                                            tACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGga                                                                                                           <  1:75586/150‑1 (MQ=255)
                                             aCAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGGTGTTGCC‑GATCCTGAAAAAGAATTTTAAGgac                                                                                                          >  2:3528266/1‑150 (MQ=255)
                                              cAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGGTGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGgacg                                                                                                         >  1:574949/1‑150 (MQ=255)
                                              cAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGG‑GGGTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGggcg                                                                                                         >  2:4044548/1‑150 (MQ=255)
                                               aGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTACCTGGGGGAGTTTTCC‑GCCCCCAAAAAAAATTTTAAAGgagg                                                                                                         >  2:2131504/1‑148 (MQ=255)
                                                 cAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAAGACGACa                                                                                                      >  1:959291/1‑150 (MQ=255)
                                                   aTGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACaaa                                                                                                    >  1:3180009/1‑150 (MQ=255)
                                                   aTGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTAACTTGGGGATGTTGCC‑GATCCTGAAAAAGATTTTTAAGGGCAACaaa                                                                                                    >  2:3146032/1‑150 (MQ=255)
                                                    tGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAAc                                                                                                   <  1:1798263/150‑1 (MQ=255)
                                                     gggATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAAcc                                                                                                  <  2:1676020/150‑1 (MQ=255)
                                                      ggATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTTGGCGATGTTTCC‑GATCCTGAAAAAAATTTATAAGGACGGCaaaacc                                                                                                 >  2:4197089/1‑147 (MQ=255)
                                                       gATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCC‑CATCCTGAAAAAGATTTATAAGGAAGACAAAcccg                                                                                                >  1:2944890/1‑148 (MQ=255)
                                                            gAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCC‑GGTCCTGAAAAAGATTTATAAGGAAGACAAACCGGGCTTa                                                                                           >  2:1586941/1‑150 (MQ=255)
                                                             aaaTCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCC‑GATCCCGAAAAAGAATTATAAGGACGACAAAACCGGCTTaa                                                                                          >  1:103335/1‑150 (MQ=255)
                                                              aaTCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAg                                                                                         <  1:3409914/150‑1 (MQ=255)
                                                              aaTCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTAACTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAg                                                                                         >  1:4026092/1‑150 (MQ=255)
                                                               aTCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTTCCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAgc                                                                                        >  1:1191014/1‑150 (MQ=255)
                                                               aTCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGGTTTCCTGGGGGGGGGTGGC‑GGCCCTGAAAAAAAATTTTAAGGAAGAACAACCGGGGTTAAgc                                                                                        >  2:3019407/1‑150 (MQ=255)
                                                               aTCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGATTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGGGATGTTGCC‑GGTCCTGAAAAAAAATTTTAAAGAAGACCAACCCGGCtttaac                                                                                        >  2:3451998/1‑146 (MQ=255)
                                                                tCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCCGGCTTAAgcg                                                                                       >  1:3019788/1‑150 (MQ=255)
                                                                 cAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGCGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAgcgc                                                                                      >  1:2545485/1‑150 (MQ=255)
                                                                   gTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCgcgg                                                                                    <  1:1482236/150‑1 (MQ=255)
                                                                        aaaGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATg                                                                               <  1:380584/150‑1 (MQ=255)
                                                                           gATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGaaa                                                                            >  1:3642135/1‑150 (MQ=255)
                                                                             tatCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTTCCTGGGCGATGTTGCC‑GGTCCTGAAAAAGATTTTTAAAGACGACAAACCCGGCTTAAGCGCGGCGATGAAAGa                                                                          >  1:3762425/1‑150 (MQ=255)
                                                                             tatCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTTCCTGGGCGATGTTGCC‑GATTCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGa                                                                          >  1:3884242/1‑150 (MQ=255)
                                                                              atCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTATCTGGGGGGTGTTTCC‑GATTCTTGAAAAGAATTTTAAAGAAGACAAAACGGGGTTAATCGCGGCGGTGAAAGAt                                                                         >  2:277177/1‑150 (MQ=255)
                                                                              atCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCC‑GGTCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGGGATGAAAAAt                                                                         >  2:199029/1‑150 (MQ=255)
                                                                               tCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGGGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAAACGGGCTTAAGCGCGGCGGTGAAAAATa                                                                        >  2:46411/1‑150 (MQ=255)
                                                                                cACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCC‑GGTCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAACGCGGCGGTGAAAGATaa                                                                       >  2:2597574/1‑150 (MQ=255)
                                                                                 aCCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATATTTCC‑GATCCTGAAAAAAATTTATAAGGATGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAAc                                                                      >  2:2286517/1‑150 (MQ=255)
                                                                                    cGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTc                                                                   <  1:2873991/150‑1 (MQ=255)
                                                                                    cGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCC‑GGTCCTGAAAAAGATTTATAAAGACGGCAAACCCGGCTTAAGCGCGGCGATGAAAGATAACCTc                                                                   >  2:1369269/1‑150 (MQ=255)
                                                                                     gTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCg                                                                  >  1:459463/1‑150 (MQ=255)
                                                                                      tCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAAACTCGa                                                                 >  1:3559641/1‑150 (MQ=255)
                                                                                      tCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTTCTGGGCGGTGTTGGC‑GGTCCTGCAAAAGATTTATAAGGGCGACCAACCGCGCTTCAACGCCGCGGTGAAAGATTACCTccg                                                                 >  2:3615545/1‑148 (MQ=255)
                                                                                            cTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATttat                                                           <  1:2575447/150‑1 (MQ=255)
                                                                                             tttCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATttatt                                                          <  1:1277293/150‑1 (MQ=255)
                                                                                             tttCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGGTGAAAGATAACCTCGAATttatt                                                          >  2:2750956/1‑150 (MQ=255)
                                                                                              ttcgttcgTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATttatta                                                         <  1:1386733/150‑1 (MQ=255)
                                                                                                cgttcgtCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAAACGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAAt                                                       >  1:3920885/1‑150 (MQ=255)
                                                                                                  ttcgtCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGGGCGGCGGTGAAAGATAACCTCGAATTTATTAATac                                                     >  2:1139078/1‑150 (MQ=255)
                                                                                                     gtcgCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTTCTGGGGGATGTTTCC‑GGTCCCGGAAAAGATTTTTAAGGGCGACAAAACTGGCTTTAGAGGGGGGGTGAAAGAAAAACCCGGATTTTTTAAAaccca                                                  >  2:3182136/1‑150 (MQ=255)
                                                                                                        gctgctGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATacccaccc                                               <  1:3138207/150‑1 (MQ=255)
                                                                                                            ctgcAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTTCCTGGGGGAGGTTGCC‑GCTACTGAAAAAGATTTTTTAGGACGACAAACCGGGGTTTAACGCGGGGATGAAAGAAAAACCCGAATTTATTAATATCCCACCGaac                                           >  2:3855900/1‑149 (MQ=255)
                                                                                                             tgcAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATc                                          <  1:4183317/150‑1 (MQ=255)
                                                                                                              gcAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGGCGACAAAACGGGCTTAAGCGCGGCGATGGAAGATAACCTCGAATTTATTAATACCCACCCGAATCt                                         >  2:2920949/1‑150 (MQ=255)
                                                                                                               cAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTg                                        <  1:2826445/150‑1 (MQ=255)
                                                                                                                  gCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTc                                     <  2:4215699/150‑1 (MQ=255)
                                                                                                                     aGCTTTAACTACGAAAGGATGCAGGCGGGG‑GGGGTTAGCTGGGGGATGGTGGC‑GGACCTGAAAAAGATTTATAAGGGCGACAAAACGGGGTTAAGCGCGGGGATGAAAGAAAACCTCGAAATTTTTAAAAACCACCCGAATCTGGTCGGa                                  >  2:1780083/1‑150 (MQ=255)
                                                                                                                      gCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGGGATTTTGCC‑CGTCCTGAAAAAGATTTTTAAGGACGACAAACCGGGCTTAAGCGCGGCGGTGAAAGATAACCTCGAATTTATTAATAACCACCCGAATCTGGTCgggt                                 >  2:1866120/1‑148 (MQ=255)
                                                                                                                      gCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCCGGGCGGTGTTGCC‑GGTCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGGGCGGCGATGAAAAAAAAACTCCGATTTATTTATACCCCCCCCAAATTGGGCgggt                                 >  2:4008194/1‑148 (MQ=255)
                                                                                                                        tttAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATTTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAAACTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTc                               >  2:1241084/1‑150 (MQ=255)
                                                                                                                          tAACTACGAAAGGATGCAGGCGGGC‑GGGTTTATCCGGGCGATGTTTCC‑GAAACTGAAAAAAAATTATAAAGACGAAAAAACGGGCTTAATCGCGGCGAGGAAAGATAACCTCGAATTTTTTTATATCCACCCGAATTTGGACGGATTTCt                             >  2:2655023/1‑150 (MQ=255)
                                                                                                                             cTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGAt                          <  1:2411580/150‑1 (MQ=255)
                                                                                                                              tACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATg                         <  2:4169721/150‑1 (MQ=255)
                                                                                                                              tACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGGTGTTGCC‑GGTCCCGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGCTGAAAGATAAACTCGAATTTATTAATACCCACCCCGATCTGGTCCGGTTCCTGATg                         >  2:3665851/1‑150 (MQ=255)
                                                                                                                                  aaaGGATGCAGGCGGGC‑GGTTTTTCCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGGCGACAAACCCGGCTTAAACGCGGCGATGAAAGATAAACTCCAATTTATTTATACCCCCCCGCATCTGGTCGGATTCCTTATGGGGt                     >  2:603637/1‑150 (MQ=255)
                                                                                                                                      gATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGttatt                 <  1:4381840/150‑1 (MQ=255)
                                                                                                                                         gCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAAt              <  1:3262300/150‑1 (MQ=255)
                                                                                                                                          cAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAAtt             >  2:759363/1‑150 (MQ=255)
                                                                                                                                                     gTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaag   <  1:2053989/150‑1 (MQ=255)
                                                                                                                                                     gTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaag   <  1:3342335/150‑1 (MQ=255)
                                                                                                                                                      ttttACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaaga  <  2:32147/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
AAGCGCAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCC‑GATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGA  >  AP012306/2758760‑2759058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑