Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,182,8610AC22.6% 388.0 / 10.9 146L198F (TTA→TTC) yiaNpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (96/17);  new base C (0/33);  total (96/50)
Fisher's exact test for biased strand distribution p-value = 1.62e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 229 total aligned reads displayed.

TTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTC‑GCTCCGGCCTG‑TTTACCCCTA‑CCGAAGCCGGGGCCGTTGCTGCTTTCTATGC  >  AP012306/3182713‑3183010
                                                                                                                                                        |                                                                                                                                                          
   ttCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCCGGCATGATGATGGGGGCAACGCTGATGCTTACCTTGT‑GGTGGGCGGCCAGGCG‑CC‑CTTATTTA‑tccc                                                                                                                                                       >  1:3407047/1‑150 (MQ=255)
    tCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCg                                                                                                                                                      <  2:1057276/150‑1 (MQ=255)
    tCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCg                                                                                                                                                      <  1:4272505/150‑1 (MQ=255)
     cccccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGc                                                                                                                                                     <  1:1717275/150‑1 (MQ=255)
     cccccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGc                                                                                                                                                     <  2:2293/150‑1 (MQ=255)
     cccccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGc                                                                                                                                                     <  1:3611974/150‑1 (MQ=255)
       cccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGGGTTGCCCCCCGCGTGATGATTGGGGCGACGCTGATGCTTTTCTTGT‑GGGGGGGGGGCAGTCC‑CCTTTATTTTA‑Ccccccc                                                                                                                                                    >  2:932688/1‑150 (MQ=255)
             cATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTTCCCTGGCATGGTGATGGGGGCGACGCTGATGCTTACCTGGT‑GGTGGGGGGCCCGTCGCCC‑TTATTTTA‑CCCC‑CCCGCaaaa                                                                                                                                             >  2:1368485/1‑150 (MQ=255)
               ttCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGGTTTTCCCCTGGC‑TGATTGTTGGGGCAACCCTGGTGCTTACCTGGT‑GGTGGGGGGCCGGTGG‑CC‑TTTATTTA‑TCCCCCCCGCcaaaaa                                                                                                                                           >  2:109781/1‑149 (MQ=255)
                tCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCGGGCCAGTCG‑CT‑TTAATTTC‑CCCCGCCAGCAAAAAGc                                                                                                                                          <  1:809404/150‑1 (MQ=255)
                     ttattaTCTTGGGCGTTCCCAGGGGATTACCCATCAGTAAGCTTTTTATGGC‑GGGCATTGCCCCTGGCATGATGAGGGGCGCACCGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTC‑CCCCGCCAGCAAAAAGCAACGa                                                                                                                                     <  2:783304/150‑1 (MQ=255)
                      tattatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGCGTTCCCCCCGG‑GTGGTGATGGGGGCAACGCTGATGCTTTCCTGGT‑GGGGGGGGGGCCGTCG‑GC‑TTTATTTT‑TCCCGCCCGCAAAAAACAACGAt                                                                                                                                    >  2:876670/1‑150 (MQ=255)
                       attatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGGCCTTCCCCTTGCCTGCTGATGGGTGGCACGCGCGTGCTTCTCCTGT‑GGGGGCTGGGCCGTCG‑GC‑CTAATTTA‑CACCCCCCCCAAAAAGAAACGaag                                                                                                                                   >  2:2897188/1‑148 (MQ=255)
                       attacCTTGGGCGTTCCCAGCGGATTATCCACCAGTAAGCTGTTTAGGGG‑GGGCATTGCCCCTGGCATGATGATGGGCGCACCGCTGATGCTTACCTGGG‑GGGGGCAGGCCAGTCG‑CT‑TTAATTTC‑CCCCGCCAGCAAAAAGCAACGATg                                                                                                                                   <  2:1621472/150‑1 (MQ=255)
                         tatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTCCCCCGGCATGATGATTGGCGCAACGCTTTTTCTTTCCTGGG‑GGGGGCCGGCCCGGCG‑GC‑TTAATTTT‑CCCCCCCAGCAAAAAACCACGATGCa                                                                                                                                 >  2:313511/1‑150 (MQ=255)
                          atCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAg                                                                                                                                <  1:432622/150‑1 (MQ=255)
                               cGGCGTTTCCCGCGGATTCTCCATCATTAAGCTGTTTAGGGC‑GGGCATCGCCCCTGGTATGATGATGGGCGCACCGGTGATTTTTACCTGGT‑GGTGGAAGGCAAGCCG‑CC‑TTATTTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAAt                                                                                                                           <  2:3368169/150‑1 (MQ=255)
                               cGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCGTTTTCCCCGGCGTGATGATGGGCGGCACGCTTTTGCTTTCCAGGA‑GGCGGCCGGCCCGCCG‑CC‑TCACTTTA‑CCCCCCCAGCCAAAAGCAACGATTAAGGaaaa                                                                                                                           >  2:1824195/1‑149 (MQ=255)
                                ggCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATc                                                                                                                          <  1:3790511/150‑1 (MQ=255)
                                 gCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCCGGGATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCCGCAAAAAGCAACGATGCAGGGAATCt                                                                                                                         >  2:2546583/1‑150 (MQ=255)
                                  cGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCGTTGCCCCTGGCATGCTTCTGGGCGGCAAGCAGCAGTTTTACCGGG‑GGGGGCAGGGCACTCG‑CC‑TTAAATTT‑CCCCCCCCCCACCAAACAACCCTGCAGGgaaaatt                                                                                                                        >  2:2383598/1‑146 (MQ=255)
                                  cGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGGCCCCGGCATGATGATGGGGGCAACGCTGATGCTTACCTGGG‑GGGGGCGGGGCAGTCG‑CC‑GTTATTTA‑TCCCCCCCCCAAAAAAGAACCAAGCAAGAAAACtt                                                                                                                        >  2:396251/1‑149 (MQ=255)
                                   gTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCGTTGCCCCCGGCCTGATGATGGGGGCCACGCTGATGCTTACCTGGG‑GGGGGGAGGGCCGGCG‑CC‑CTAATTTT‑ACCCCCCCGCAAAAAAAAAACAAGCAGGAAAACTgg                                                                                                                       >  2:1684592/1‑150 (MQ=255)
                                   gTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCAATGCCCCCGGCATGAAGAAGGGGGCAAAGCCGATGCTTACCCGGT‑GGGGGGGGGGCAGGCG‑CC‑CTAATTTT‑ACCCCCCCGCAAAAAACAACGGAGCAGGGAAACttg                                                                                                                       >  2:963062/1‑148 (MQ=255)
                                    tttCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTCCCCCCGGATGATGATGGGCGCAACGCTGATGCTTTTCTGGG‑GGGGGGAGGCCAGGCG‑CC‑CTAATTTA‑CCCCCCCCGCAAAAAACAACGATGCAGGGAATCTTGc                                                                                                                      >  2:1236152/1‑150 (MQ=255)
                                     ttCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTCCCTGGT‑GGTGGCAGGCCAGTCC‑CC‑TTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCa                                                                                                                     <  1:3758252/150‑1 (MQ=255)
                                      tCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCCGGCCTGATGATGGGGGCAACGCTGATGCTTACCTGGT‑GGGGGGAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAACAACGATGCAGGAAATATGGCAc                                                                                                                    >  2:4126352/1‑150 (MQ=255)
                                          gCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGGATTGCCCCCGGCATGATGATGGGGGCAACGCTGATGGTTTCCTGGT‑GGGGGGAGGCCCGGGG‑CC‑TTAATTTT‑ACCCCCCCGCCAAAAACAAAGAAGCCGGAAATCTGGCACT‑Ctt                                                                                                               >  2:3884781/1‑150 (MQ=255)
                                            ggATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCGGGCATGATGATGGGCGCACCGCTGATCCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CT‑TTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑Ctttt                                                                                                             <  2:4168255/150‑1 (MQ=255)
                                            ggATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCCCGCATGATTATGGGGGCCACGCTGATGCCTTCCTGGT‑GGTGGGCGGCCCGTCGCCC‑TCAATTAA‑CCCCCCCA‑CAAAAAACAACCAAGCAGGAAATCTGGTACC‑Ccttt                                                                                                             >  2:720514/1‑150 (MQ=255)
                                              aTTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CT‑TTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGt                                                                                                           <  1:3845605/150‑1 (MQ=255)
                                              aTTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGt                                                                                                           <  1:1481636/150‑1 (MQ=255)
                                               ttATCCATCAGTAAGCTGTTTATGGC‑GGGGATTTCCCCCGGGATTATGATTGGGGGAACGCTGATGCTTAACTTGG‑GGGGGGAGGCCAATCG‑CC‑CTTATTTT‑CCCCCCCCGCAAAAAACAAAGATGCAGGAAAACTGGCACT‑Cttttttt                                                                                                          >  2:624459/1‑147 (MQ=255)
                                               ttATCCATCAGTAAGCTGTTTATGGC‑GGGCCATGCCCCCGGCCTCGTGATGGGGGCGACCCATCTGCCTACCTTGT‑GGGGGCGGGGCAGGGG‑CC‑CTATTTTA‑CCCCCCCAGCCAAAAACAACAAAGGAGGAAATCTGGCACA‑CTTTTGtc                                                                                                          >  2:2996634/1‑150 (MQ=255)
                                                tATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTCCCCCCGCCTGATGATGGGGGCGACGCTGATGCTTTCCTTGT‑GGGGGCAGGGCCGTCC‑CC‑TTTATTTT‑CCCCCCCCGCAAAAAACAACGAAGCAGGGAATCTGGCACT‑CTTTTTtct                                                                                                         >  2:2625283/1‑150 (MQ=255)
                                                 aTCCATCAGTAAGCTGTTTATGGC‑GGGGATTTCCCCCGGCCTGTTGATGGGGGCAACGCTGATGCTTTCCTTGG‑GGGGGGGGGGCCGGCG‑CC‑TTTATTTA‑CCCCCCCCGCAAAAAAGAAAGAAGGAGGGAATCTGGGACT‑CTTTTTtctc                                                                                                        >  2:2786767/1‑150 (MQ=255)
                                                 aTCCATCAGTAAGCTGTTTATGGC‑GGGCACTTCCCCCCGCGTGGTGATGGGCGCGACCCCGATGGTTTTCTTGTGGGGGGCGGGCCGCCGG‑CC‑TTATTTTA‑CCCCCCCCCCAAAAA‑AAAAGAAGCAGGAAAACTGGGCAT‑Cttttttttt                                                                                                        >  2:132191/1‑145 (MQ=255)
                                                   ccATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCg                                                                                                      <  2:2690134/150‑1 (MQ=255)
                                                          tAAGCTGTTTAGGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGTTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTGTCTg                                                                                               <  1:611870/150‑1 (MQ=255)
                                                           aaGCTGTTTATGGC‑GGGCATTACCCCCCGCCTGATGGTGGGGGCAACGCTGGTGTTTTCCTGGG‑GGTGGGAGGCCAGTCGCCC‑TTATTTTA‑CCCC‑CCCGCAAAAAACAACGAAGCAGGGAAACTGGGGCTCCTTTTTTTTCCggatcttg                                                                                               >  2:3249353/1‑144 (MQ=255)
                                                                gTTTATGGC‑GGGCATTGCCCCCGGCCTGGGGGTGGGGGGAACGCTGGTGGTTTCCCGGG‑GGGGGGAGGGCCGGCG‑CC‑CTAATTTT‑ACCCCCCCGCAAAAAACAAAGAAGCAGGGAAACCGGGACC‑CTTTTTTCTTCCGGATCTTGgggcc                                                                                         >  2:1526734/1‑149 (MQ=255)
                                                                gTTTATGGC‑GGGCATGGCCCGTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCt                                                                                         <  1:954300/150‑1 (MQ=255)
                                                                      ggC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTttct                                                                                   >  1:4091839/1‑150 (MQ=255)
                                                                      ggC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTttct                                                                                   >  2:2399925/1‑150 (MQ=255)
                                                                        c‑GGGCATTGTCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTttcttc                                                                                 >  2:563695/1‑150 (MQ=255)
                                                                          gggCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTcc                                                                                >  1:1168925/1‑150 (MQ=255)
                                                                          gggCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTcc                                                                                >  2:1536863/1‑150 (MQ=255)
                                                                          gggCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCGGGT‑GGTGGCAGGCCAGTCG‑CT‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTcc                                                                                <  1:2616441/150‑1 (MQ=255)
                                                                           ggCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCa                                                                               >  2:3944862/1‑150 (MQ=255)
                                                                           ggCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCa                                                                               >  1:2816965/1‑150 (MQ=255)
                                                                              aTTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtc                                                                            >  2:633353/1‑150 (MQ=255)
                                                                              aTTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtc                                                                            >  2:1488355/1‑150 (MQ=255)
                                                                              aTTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtc                                                                            >  1:1031281/1‑150 (MQ=255)
                                                                                  ccccTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatca                                                                        >  1:1066387/1‑150 (MQ=255)
                                                                                  ccccTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatca                                                                        >  1:1436301/1‑150 (MQ=255)
                                                                                  ccccTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatca                                                                        <  1:199526/150‑1 (MQ=255)
                                                                                  ccccTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatca                                                                        >  1:2139984/1‑150 (MQ=255)
                                                                                   cccTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTcatcat                                                                       <  1:159286/150‑1 (MQ=255)
                                                                                   cccTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTcatcat                                                                       >  2:2456651/1‑150 (MQ=255)
                                                                                   cccTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTcatcat                                                                       >  2:2580746/1‑150 (MQ=255)
                                                                                    ccTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCatt                                                                      >  1:3990281/1‑150 (MQ=255)
                                                                                      tGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattat                                                                    >  2:3047848/1‑150 (MQ=255)
                                                                                       ggCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattatt                                                                   >  2:126658/1‑150 (MQ=255)
                                                                                        gCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTg                                                                  >  1:4179680/1‑150 (MQ=255)
                                                                                           tgatgatgGGCGCACCGCTGATGCTTCCCTGGT‑GGTGGCAGGCCAGTCG‑CT‑TTATTTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCg                                                               <  2:958054/150‑1 (MQ=255)
                                                                                            gatgatgGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCgg                                                              <  1:1951278/150‑1 (MQ=255)
                                                                                             atgatgGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCggg                                                             >  1:3545636/1‑150 (MQ=255)
                                                                                             atgaggGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGCCCAGTCG‑CT‑TTATTTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCggg                                                             <  2:3077983/150‑1 (MQ=255)
                                                                                                atgGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGgtt                                                          >  2:3538873/1‑150 (MQ=255)
                                                                                                       cAACGCTGATGCTTCCCTGGT‑GGTGGCAGCCCAGTCG‑CT‑TTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCTGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTC‑GCTCCg                                                  <  2:438137/150‑1 (MQ=255)
                                                                                                       cAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTT‑GCTCCg                                                  >  1:174856/1‑150 (MQ=255)
                                                                                                        aaCGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTC‑GCTcccc                                                 >  1:234581/1‑148 (MQ=255)
                                                                                                        aaCGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTT‑TCTcccg                                                 >  1:3963422/1‑148 (MQ=255)
                                                                                                        aaCGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTT‑GCTcctg                                                 >  2:3607895/1‑148 (MQ=255)
                                                                                                        aaCGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTT‑GCTcccg                                                 >  1:2757711/1‑148 (MQ=255)
                                                                                                           gCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTT‑TCTCCGGCCt                                              >  2:1810784/1‑150 (MQ=255)
                                                                                                              gATGCTTACCTGGT‑GGGGGCAGGCCAGTCG‑CT‑TTATTTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTC‑GCTCCGGCCTG‑tt                                          <  2:4264162/150‑1 (MQ=255)
                                                                                                                     aCCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTT‑GCTCCGGCCTG‑ttttctccc                                   >  2:1950709/1‑144 (MQ=255)
                                                                                                                      ccTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTATTTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTC‑GCTCCGGCCTG‑TTTACCCCTa                                  <  2:3908188/150‑1 (MQ=255)
                                                                                                                       cgggt‑ggtggCAGGCCAGTCG‑CT‑TTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTC‑GCTCCGGCCTG‑TTTACCCCTA‑c                                <  1:457629/148‑1 (MQ=255)
                                                                                                                       cTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTT‑GCTCCGGGCTG‑TTTATCCCCA‑c                                >  1:4057072/1‑150 (MQ=255)
                                                                                                                       cTGGT‑GGGGGAGGCCCGGTCG‑CC‑TTAATTGA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTC‑GCTCCGGCCTG‑TTTACCCCTA‑c                                <  1:3431092/150‑1 (MQ=255)
                                                                                                                         ggt‑ggtggCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTT‑TCTCCCGCCTC‑TTTTTCCCCA‑CCg                              >  2:3752875/1‑150 (MQ=255)
                                                                                                                           t‑ggtggCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTC‑GCTCCGGCCTG‑TTTTCCCCTA‑CCGaa                            >  1:3907035/1‑150 (MQ=255)
                                                                                                                                ggCGGGCCAGCCC‑CT‑TTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑TTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCACTATTGGCGGGTTTC‑GCTCCGGCCTG‑TTTACCCCTA‑CCGAAGCCg                        <  2:3407189/150‑1 (MQ=255)
                                                                                                                                ggCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTC‑GCTCCCGCCCG‑GTTTACCCTA‑CCCAAGccc                        >  1:410325/1‑149 (MQ=255)
                                                                                                                                     gCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTT‑TCTCCCGGCTGTTTTTCCCCCC‑CCCAACCCGGGGc                    >  2:3676186/1‑150 (MQ=255)
                                                                                                                                      ccAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTC‑GCTCCCGCCTG‑TTTACCCCTA‑CCCAAGCCCGGGCCg                  >  1:2385092/1‑150 (MQ=255)
                                                                                                                                        aGTCG‑CT‑TTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTC‑GCTCCGGCCTG‑TTTACCCCTA‑CCGAAGCCGGGGCCGtt                <  1:2956490/150‑1 (MQ=255)
                                                                                                                                         gTCG‑CC‑TTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTC‑GCTCCGGCCTG‑TTTACCCCTA‑CCGAAGCCGGGGCCGTtg               <  1:670827/150‑1 (MQ=255)
                                                                                                                                         gTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTT‑GCTCCGGCCCG‑TTTATCCCTA‑CCCCAGCCCGGGGCGTtg               >  1:2580261/1‑150 (MQ=255)
                                                                                                                                          tCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTT‑GCTCCGGCCTG‑TTTTTCCCTACCCGAGCCCGGGGCGGttc               >  1:2242970/1‑149 (MQ=255)
                                                                                                                                          tCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTC‑GCTCCCGCCCG‑CTTTCCCCCA‑CCCCAGCCCGGGCCgccgc              >  2:464003/1‑146 (MQ=255)
                                                                                                                                               c‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTT‑GCTCCGGCCCG‑TTTTTCCCCC‑CCCCAACCCGGGCCGTTgcttct          >  2:3977009/1‑150 (MQ=255)
                                                                                                                                                 ttAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCTGTTCCGCCCCT‑TTTCCCCCCC‑CCAAACCCCGGG‑CGGTGCTGCtt         >  2:2219128/1‑150 (MQ=255)
                                                                                                                                                    aTTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTC‑GCTCCGGCCTG‑TTTACCCCTA‑CCGAAGCCGGGGCCGTTGCTGCTTTCt      <  1:1318627/150‑1 (MQ=255)
                                                                                                                                                    aTTTACCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTC‑GCTCCGGCCTG‑TTTACCCCTA‑CCGAAGCCGGGGCCGTTGCTGCTTTc       <  2:4209436/150‑1 (MQ=255)
                                                                                                                                                     tttA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTC‑GCTCCGGCCTG‑TTTACCCCTA‑CCGAAGCCGGGGCCGTTGCTGCTTTCTa     <  1:1637932/150‑1 (MQ=255)
                                                                                                                                                     tttA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTC‑GCTCCGGCCTG‑TTTACCCCTA‑CCGAAGCCGGGGCCGTTGCTGCTTTCTa     <  1:1137524/150‑1 (MQ=255)
                                                                                                                                                        a‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGATTT‑TCTCCCGCCCG‑TTTTCCCCCC‑CCCCAGCCGCGGCCGGGGCTGCttttctttg  >  2:2060257/1‑144 (MQ=255)
                                                                                                                                                        |                                                                                                                                                          
TTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGT‑GGTGGCAGGCCAGTCG‑CC‑TTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACT‑CTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTC‑GCTCCGGCCTG‑TTTACCCCTA‑CCGAAGCCGGGGCCGTTGCTGCTTTCTATGC  >  AP012306/3182713‑3183010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑