Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,527,3680AC28.8% 628.4 / 58.1 278L140F (TTA→TTC) rhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
Reads supporting (aligned to +/- strand):  ref base A (161/37);  new base C (0/80);  total (161/117)
Fisher's exact test for biased strand distribution p-value = 2.66e-41
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 326 total aligned reads displayed.

CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTG  >  AP012306/3527219‑3527517
                                                                                                                                                      |                                                                                                                                                     
cGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTa                                                                                                                                                       <  1:189142/150‑1 (MQ=255)
 gATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTAc                                                                                                                                                      >  2:927648/1‑150 (MQ=255)
 gATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTAc                                                                                                                                                      >  2:3195514/1‑150 (MQ=255)
  aTTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTAcc                                                                                                                                                     <  2:3779650/150‑1 (MQ=255)
    ttCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCt                                                                                                                                                   >  1:3684018/1‑150 (MQ=255)
     tCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTc                                                                                                                                                  <  2:3813537/150‑1 (MQ=255)
      cAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCg                                                                                                                                                 >  2:1050293/1‑150 (MQ=255)
        gTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGAc                                                                                                                                               >  2:1202904/1‑150 (MQ=255)
         tttCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGAcc                                                                                                                                              >  2:3939114/1‑150 (MQ=255)
          ttCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCg                                                                                                                                             >  2:1752765/1‑150 (MQ=255)
              cGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAg                                                                                                                                         <  2:2738038/150‑1 (MQ=255)
              cGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAg                                                                                                                                         <  1:1359022/150‑1 (MQ=255)
                     cTGAATCAGTTGCTGCCACAAGAGCTGGATGGTCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGAcc                                                                                                                                              >  1:1766653/1‑138 (MQ=255)
                       gAATCGGTTGCTGCCACAAGGGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGGATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGAt                                                                                                                                <  1:3644357/150‑1 (MQ=255)
                       gAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGAt                                                                                                                                <  1:3443538/150‑1 (MQ=255)
                         aTCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCt                                                                                                                              >  1:3174062/1‑150 (MQ=255)
                          tCATTTCCTGCAACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGAAACAGCAGGAAGGGA‑AAAATAATTTCCCCTCGACCGCCAGTCGCGAGATCtt                                                                                                                             <  2:4164027/150‑1 (MQ=255)
                          tCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCtt                                                                                                                             >  1:1609478/1‑150 (MQ=255)
                            aGTTGCTGCCAAAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACGGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGt                                                                                                                           <  1:3561682/150‑1 (MQ=255)
                              ttGCTGCCACAAGGGCTGGATGGGCAGTTTCCGTCTCACTGGCGCTTTACCCACAGCGTATTGCAGCAGGTGCGCCAGCTGGTTCCACAGATGGAACGGAAGGAGGGGG‑AAAATGATTTCCCCCCGACCGCCAGTCGCGAGATCTTGttt                                                                                                                         <  2:3105255/150‑1 (MQ=255)
                                gctgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTAt                                                                                                                       <  1:2456631/150‑1 (MQ=255)
                                gctgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTAt                                                                                                                       >  1:921382/1‑150 (MQ=255)
                                  tgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGc                                                                                                                     >  1:813634/1‑150 (MQ=255)
                                    ccACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCaa                                                                                                                   >  2:1330675/1‑150 (MQ=255)
                                    ccACAAGAGCTGGATGGGCAGTATCCGTCTCACGGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGA‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCaa                                                                                                                   <  2:2777681/150‑1 (MQ=255)
                                      acaAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAAtt                                                                                                                 >  1:734291/1‑150 (MQ=255)
                                      aaaaGGGCTGGATGGGAAGTACCCGTTCCCCGGGCGCGTTAACCACGGCGTTTTGCAGCAGGTGCGCCAGCTGGTTGCCCAGAGGAAAAAGAAGGAGGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAAtt                                                                                                                 <  2:40572/148‑1 (MQ=255)
                                       caAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTa                                                                                                                >  1:2708849/1‑150 (MQ=255)
                                        aaGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGA‑AAAATTTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTAc                                                                                                               <  2:2834027/150‑1 (MQ=255)
                                         agagCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACt                                                                                                              >  2:171411/1‑150 (MQ=255)
                                          gagCTGGAGGGGCAGTACCCGTCCCACGGGCGCGTAAACCACAGCGTATGGCAGCAGGTGCGACAGCGGGTTGCACAGATGAAAAAGGAGGAAGGGA‑AAAAGGTTTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTg                                                                                                             <  2:2805743/150‑1 (MQ=255)
                                            gCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGct                                                                                                           <  1:2168941/150‑1 (MQ=255)
                                            gCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGct                                                                                                           >  2:4234282/1‑150 (MQ=255)
                                             cTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctc                                                                                                          >  1:3032625/1‑150 (MQ=255)
                                               ggATGGGCAGTACCCGTCTCACGGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGAGGGAACAGCAGGAAGGGA‑AAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCtt                                                                                                        <  2:3895639/150‑1 (MQ=255)
                                               ggAGGGGCAGTACCCGTCTCCCTGGCGCGTTAACCACGGCGTATTGCAGCAGGGGCGACAGTTGGTTGCACAGAGGGAACAGCAGGAGGGGA‑AAAATTTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCtt                                                                                                        <  2:4346957/150‑1 (MQ=255)
                                                gATGGGAAATTTCCTTCTCACGGGCTCGTTAACCAGAGTGTTTTGCAGCGGGGGCGACAGCTGGTTGCAAAGATGAAACAGCAGGAAGGGG‑AAAATTATTTCCCCGCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtg                                                                                                       <  2:3969331/150‑1 (MQ=255)
                                                 acgggCAGTCTCCTGCTCCCTGGCGCGTCCACTCCAGCTTTTTCCCAAACGTGCGACGGCGGGCTGCACAGTGGGAACCGTAGGAAGGGA‑AAAGTTATTCTCCCTCCCCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgc                                                                                                      <  2:3686163/148‑1 (MQ=255)
                                                    ggCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgctgc                                                                                                   >  1:2243926/1‑150 (MQ=255)
                                                      cAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGt                                                                                                 >  1:528915/1‑150 (MQ=255)
                                                       aGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTa                                                                                                >  2:2876985/1‑150 (MQ=255)
                                                         tATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaa                                                                                              <  1:1126301/150‑1 (MQ=255)
                                                         tATCCGTCTCACGGGCGCGTTAACCACAGCGTATGGCAGCGGGGGCGACAGCTGGTTGCACAGATGGAACAGCAGGAGGGGA‑AAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaa                                                                                              <  2:1978068/150‑1 (MQ=255)
                                                              gTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAg                                                                                         >  1:4374529/1‑150 (MQ=255)
                                                               tctcAGGGGCGCGTTAACCAGAGCGTATGGAAGGAGGTGCGCCAGTTGGTTGCACAGATGGACCAGCAGGAGGGGG‑AAAATAATTCCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGt                                                                                        <  2:1559622/150‑1 (MQ=255)
                                                               tctcACTGGCGCGTTACCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGt                                                                                        <  2:689074/150‑1 (MQ=255)
                                                                ctcACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGtt                                                                                       >  1:1028952/1‑150 (MQ=255)
                                                                ctcACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGtt                                                                                       >  1:1977157/1‑150 (MQ=255)
                                                                  cACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGAAGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTg                                                                                     >  2:4125150/1‑150 (MQ=255)
                                                                    cgggCGCGTTAACCACAGCTTATTGCAGCGGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCGCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCa                                                                                   <  2:3347914/148‑1 (MQ=255)
                                                                     tGGCGCGTGAACCACAGCGTATGGCAGCAGGTGCGACAGCTGGTTGCACAGAGGGAACAGGAGGAAGGGA‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAg                                                                                  <  2:535843/150‑1 (MQ=255)
                                                                       gcgcGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGga                                                                                <  1:4079021/150‑1 (MQ=255)
                                                                           gTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAAc                                                                            >  2:235154/1‑150 (MQ=255)
                                                                            ttAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAAcc                                                                           <  1:2465226/150‑1 (MQ=255)
                                                                             tagccACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCt                                                                          >  2:3840459/4‑150 (MQ=255)
                                                                              aaCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTg                                                                         >  1:3482518/1‑150 (MQ=255)
                                                                                ccACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAGGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGa                                                                       <  1:2326845/150‑1 (MQ=255)
                                                                                     gCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACa                                                                  <  1:288652/150‑1 (MQ=255)
                                                                                       ttATTGCAGCAGGGGCGACAGTTGGTGGCACAGATGGAACAGCAGGAGGGGA‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAgc                                                                <  2:1906166/149‑1 (MQ=255)
                                                                                            gcagcaGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATc                                                           >  2:825837/1‑150 (MQ=255)
                                                                                             gagcagGTGCGACAGCTGGTGGCACAGAAGGAAAAGCAGGAGGGAG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCa                                                          <  2:2468420/149‑1 (MQ=255)
                                                                                             cagcagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCa                                                          >  2:1808706/1‑150 (MQ=255)
                                                                                              agcagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAc                                                         >  2:2518162/1‑150 (MQ=255)
                                                                                              agcagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAc                                                         >  1:3384204/1‑150 (MQ=255)
                                                                                                 gggTGCGACAGCTGGTGGCACAGAGGGAACAGCAGGAGGGGA‑AAAATGTTTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGtc                                                      <  1:4190016/149‑1 (MQ=255)
                                                                                                 agGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGtc                                                      <  1:4054703/150‑1 (MQ=255)
                                                                                                   gTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGtctc                                                    >  1:2799370/1‑150 (MQ=255)
                                                                                                       gACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACt                                                >  2:25783/1‑150 (MQ=255)
                                                                                                       gACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACt                                                >  2:1662314/1‑150 (MQ=255)
                                                                                                       gACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACt                                                >  1:2522761/1‑150 (MQ=255)
                                                                                                         cAGCGGGTTGAACAGAGGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTg                                              <  1:1091616/150‑1 (MQ=255)
                                                                                                            atGGTTCCACAGAGGAAACAGCAGGAGGGGA‑AAAATTATTTCCCCTCTACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCtt                                           <  2:2096589/149‑1 (MQ=255)
                                                                                                              ggTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGACCCTGGAAAACAGCGCATCACGTCTCAACTTGCTTct                                         <  1:3839142/150‑1 (MQ=255)
                                                                                                                 tGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggc                                      <  1:1505846/150‑1 (MQ=255)
                                                                                                                 tGCACAGAGGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggc                                      <  1:1194998/150‑1 (MQ=255)
                                                                                                                  gCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcc                                     >  1:603041/1‑150 (MQ=255)
                                                                                                                  gCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcc                                     >  2:2642445/1‑150 (MQ=255)
                                                                                                                  gCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcc                                     >  2:1940804/1‑150 (MQ=255)
                                                                                                                   cacaGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcct                                    >  2:4408775/1‑150 (MQ=255)
                                                                                                                     caGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGt                                                       <  2:611642/129‑1 (MQ=255)
                                                                                                                     caGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctgg                                  >  1:3936957/1‑150 (MQ=255)
                                                                                                                        agggAACAGCAGGAAGGGG‑AAAATGTTTACCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCctggctg                               <  1:2089430/148‑1 (MQ=255)
                                                                                                                        aTGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCctggctg                               >  1:990945/1‑150 (MQ=255)
                                                                                                                        aTGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGCAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCctggctg                               >  2:3475869/1‑150 (MQ=255)
                                                                                                                         tGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCctggctgg                              >  2:773365/1‑150 (MQ=255)
                                                                                                                          ggAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTgga                             >  1:1651398/1‑150 (MQ=255)
                                                                                                                           gAACGGTAGGAAGGGGAAAAATGATTGGCCGCGGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTgga                             <  2:4298615/150‑1 (MQ=255)
                                                                                                                            aaCAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGATATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggagg                           >  1:4226002/1‑150 (MQ=255)
                                                                                                                               agcagGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGAcc                        >  2:2955980/1‑150 (MQ=255)
                                                                                                                                 cagGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAt                      >  2:1339797/1‑150 (MQ=255)
                                                                                                                                      aGGGG‑AAAATGATTTGCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGc                 <  2:2309053/150‑1 (MQ=255)
                                                                                                                                       gggg‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGcc                >  2:2196981/1‑150 (MQ=255)
                                                                                                                                        ggg‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCg               <  1:4130774/150‑1 (MQ=255)
                                                                                                                                        ggg‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCg               >  1:3274740/1‑150 (MQ=255)
                                                                                                                                         gg‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGa              <  2:1256069/150‑1 (MQ=255)
                                                                                                                                          g‑AAAATGTTTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGAt             <  2:3616936/150‑1 (MQ=255)
                                                                                                                                           aaaaaTGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGAt             <  1:1826198/149‑1 (MQ=255)
                                                                                                                                             aaaTGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGa           >  2:3253916/1‑150 (MQ=255)
                                                                                                                                                tGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGt        <  2:290713/150‑1 (MQ=255)
                                                                                                                                                  aTTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGa      >  2:3029827/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTG  >  AP012306/3527219‑3527517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑