BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	343,408	G→A	R11H (CGT→CAT)	thiL →	thiamin‑monophosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	343,408	0	G	A	100.0%	1039.5 / NA	266	R11H (CGT→CAT)	thiL	thiamin‑monophosphate kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (115/151); total (115/151)

Only 100 of 270 total aligned reads displayed.

GATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCG  >  AP012306/343261‑343554

gATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCAtt                                                                                                                                                  >  2:3696017/1‑150 (MQ=255)

 aTAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTa                                                                                                                                                 <  1:3482767/150‑1 (MQ=255)

  tAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTAt                                                                                                                                                <  2:4048348/150‑1 (MQ=255)

   aaaGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCattatt                                                                                                                                               <  1:1523806/150‑1 (MQ=255)

    aaGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTAttt                                                                                                                                              <  1:1200550/150‑1 (MQ=255)

       cagcaCCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGa                                                                                                                                           <  1:866284/150‑1 (MQ=255)

         gcaCCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGAcc                                                                                                                                         <  2:1627634/150‑1 (MQ=255)

           aCCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCgt                                                                                                                                       >  2:2940077/1‑150 (MQ=255)

                tgATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTaag                                                                                                                                  >  2:633791/1‑150 (MQ=255)

                 gATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTaaga                                                                                                                                 >  1:203602/1‑150 (MQ=255)

                  aTTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTaagaa                                                                                                                                >  2:2540593/1‑150 (MQ=255)

                  aTTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTaagaa                                                                                                                                >  2:1398819/1‑150 (MQ=255)

                   ttCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTaagaag                                                                                                                               <  1:850095/150‑1 (MQ=255)

                    tCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACATGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGt                                                                                                                              >  1:3817022/1‑150 (MQ=255)

                      gCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTtc                                                                                                                            <  1:3190586/150‑1 (MQ=255)

                      gCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTtc                                                                                                                            <  2:1028449/150‑1 (MQ=255)

                          tAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGt                                                                                                                        <  2:3416141/150‑1 (MQ=255)

                          tAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGt                                                                                                                        <  2:4183780/150‑1 (MQ=255)

                          tAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGt                                                                                                                        <  2:2197116/150‑1 (MQ=255)

                           aaCAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTc                                                                                                                       >  2:1131505/1‑150 (MQ=255)

                              aaaaaGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTg                                                                                                                    >  1:2150719/1‑150 (MQ=255)

                              aaaaaGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTg                                                                                                                    >  1:1730762/1‑150 (MQ=255)

                              aaaaaGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTg                                                                                                                    <  2:3091270/150‑1 (MQ=255)

                               aaaaGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGa                                                                                                                   >  1:1625810/1‑150 (MQ=255)

                               aaaaGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCCCGTCTTGa                                                                                                                   >  1:1366051/1‑150 (MQ=255)

                                 aaGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATg                                                                                                                 <  1:4282741/150‑1 (MQ=255)

                                     gATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGa                                                                                                             >  2:1295806/1‑150 (MQ=255)

                                        atCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACt                                                                                                          >  1:2638549/1‑150 (MQ=255)

                                          ccAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTgg                                                                                                        <  1:2009535/150‑1 (MQ=255)

                                          ccAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTgg                                                                                                        >  2:3116563/1‑150 (MQ=255)

                                          ccAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTgg                                                                                                        >  1:3007351/1‑150 (MQ=255)

                                            aGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGc                                                                                                      <  2:264147/150‑1 (MQ=255)

                                            aGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGc                                                                                                      >  2:3292341/1‑150 (MQ=255)

                                              gCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCAt                                                                                                    <  1:3467864/150‑1 (MQ=255)

                                                 ggTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCgg                                                                                                 >  1:1661740/1‑150 (MQ=255)

                                                    aGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGcga                                                                                              >  1:2844130/1‑150 (MQ=255)

                                                     gATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGcgac                                                                                             <  2:1590475/150‑1 (MQ=255)

                                                     gATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGcgac                                                                                             <  1:977000/150‑1 (MQ=255)

                                                         cACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACTGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGAtt                                                                                         >  2:828150/1‑150 (MQ=255)

                                                         cACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGAtt                                                                                         >  2:1099250/1‑150 (MQ=255)

                                                         cACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGAtt                                                                                         >  2:1214909/1‑150 (MQ=255)

                                                         cACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGAtt                                                                                         <  2:3756448/150‑1 (MQ=255)

                                                            ggAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTgcg                                                                                      >  2:560085/1‑150 (MQ=255)

                                                              aaGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCa                                                                                    <  2:1700520/150‑1 (MQ=255)

                                                                gACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACt                                                                                  <  2:1768442/150‑1 (MQ=255)

                                                                 aCCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACtt                                                                                 >  1:1384230/1‑150 (MQ=255)

                                                                    gTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTtctc                                                                              >  1:552262/1‑150 (MQ=255)

                                                                    gTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTtctc                                                                              <  2:3260601/150‑1 (MQ=255)

                                                                     ttCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCa                                                                             <  1:4038759/150‑1 (MQ=255)

                                                                     ttCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCa                                                                             <  1:4328457/150‑1 (MQ=255)

                                                                       ccATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAat                                                                           <  2:3444849/150‑1 (MQ=255)

                                                                         aTGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAatat                                                                         >  2:2701684/1‑150 (MQ=255)

                                                                         aTGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAatat                                                                         <  2:2496049/150‑1 (MQ=255)

                                                                            aTCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGAATGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTGTTGATGTCGAACTGGGCATCGGCGGCGGTTGCGCAGTTCTCAATATccc                                                                      >  2:2876727/1‑150 (MQ=255)

                                                                             tCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATcccc                                                                     >  1:3904288/1‑150 (MQ=255)

                                                                               gCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCga                                                                   >  1:3515896/1‑150 (MQ=255)

                                                                                  ggCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGaa                                                                <  1:515760/150‑1 (MQ=255)

                                                                                  ggCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGaa                                                                >  1:4186749/1‑150 (MQ=255)

                                                                                  ggCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGaa                                                                <  2:3234621/150‑1 (MQ=255)

                                                                                  ggCCTTTTCTGTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGaa                                                                <  1:4376615/150‑1 (MQ=255)

                                                                                    ccTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAAc                                                              >  1:2502575/1‑150 (MQ=255)

                                                                                     cttttcttttTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACa                                                             <  2:1774517/150‑1 (MQ=255)

                                                                                        ttcttttTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGACGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGAc                                                          <  1:3958885/150‑1 (MQ=255)

                                                                                          cttttTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGAccc                                                        >  1:1934512/1‑150 (MQ=255)

                                                                                             ttttACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTgg                                                     <  1:1067050/150‑1 (MQ=255)

                                                                                                tACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGa                                                  <  1:2976743/150‑1 (MQ=255)

                                                                                                      ctgAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCa                                            <  1:2442265/150‑1 (MQ=255)

                                                                                                       tgAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCAc                                           >  2:2369264/1‑150 (MQ=255)

                                                                                                        gAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACt                                          >  2:3483859/1‑150 (MQ=255)

                                                                                                        gAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACt                                          >  1:1122169/1‑150 (MQ=255)

                                                                                                        gAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACt                                          >  1:1630876/1‑150 (MQ=255)

                                                                                                         aGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTg                                         >  2:4067802/1‑150 (MQ=255)

                                                                                                           gCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGAt                                       <  2:3932963/150‑1 (MQ=255)

                                                                                                             aTAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTAGCGATCAGCACTGATAc                                     <  2:3608367/150‑1 (MQ=255)

                                                                                                              tAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACg                                    >  2:3600581/1‑150 (MQ=255)

                                                                                                               aaCGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGc                                   <  2:1500383/150‑1 (MQ=255)

                                                                                                                aCGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCt                                  <  2:2873726/150‑1 (MQ=255)

                                                                                                                aCGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCt                                  <  2:1310420/150‑1 (MQ=255)

                                                                                                                 cGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCtg                                 <  1:375807/150‑1 (MQ=255)

                                                                                                                    aTGGCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCtggtg                              <  1:2275358/150‑1 (MQ=255)

                                                                                                                      ggCATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGc                            >  1:63880/1‑150 (MQ=255)

                                                                                                                        cATGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCgg                          >  1:2936598/1‑150 (MQ=255)

                                                                                                                         aTGTGGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCggg                         >  1:2073576/1‑150 (MQ=255)

                                                                                                                          tgtgGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGt                        <  2:2607676/150‑1 (MQ=255)

                                                                                                                           gtgGCGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTa                       <  2:2006511/150‑1 (MQ=255)

                                                                                                                               cGAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCa                   >  1:2747852/1‑150 (MQ=255)

                                                                                                                                gAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCAt                  <  1:3285029/150‑1 (MQ=255)

                                                                                                                                gAGTTCTCCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCAt                  <  2:2322973/150‑1 (MQ=255)

                                                                                                                                     ctcCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTtcct             <  1:1971588/150‑1 (MQ=255)

                                                                                                                                     ctcCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTtcct             <  2:2195532/150‑1 (MQ=255)

                                                                                                                                     ctcCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTtcct             >  1:760590/1‑150 (MQ=255)

                                                                                                                                     ctcCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGGAACCATTtcct             >  2:880905/1‑150 (MQ=255)

                                                                                                                                      tcCCTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTtcctc            >  1:4215028/1‑150 (MQ=255)

                                                                                                                                       cccTGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTtcctcc           <  1:2700472/150‑1 (MQ=255)

                                                                                                                                          tGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTg        <  1:2969692/150‑1 (MQ=255)

                                                                                                                                          tGATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTg        <  1:3544867/150‑1 (MQ=255)

                                                                                                                                           gATTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGa       <  1:1793694/150‑1 (MQ=255)

                                                                                                                                            aTTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGat      <  1:3666699/150‑1 (MQ=255)

                                                                                                                                            aTTGCCCATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGat      <  2:4343870/150‑1 (MQ=255)

                                                                                                                                                cccATTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCg  >  2:4223568/1‑150 (MQ=255)

GATAAAGCAGCACCTGTGATTCGCCCTAACAAAAAGTGATATCCAGGCCGGTAGATTCACGGAAGACCGTTCCATGATCGCCGGCCTTTTCTTTTTTACCTGCTGAGGCATAACGTATGGCATGTGGCGAGTTCTCCCTGATTGCCCGTTATTTTGACCGTGTAAGAAGTTCTCGTCTTGATGTCGAACTGGGCATCGGCGACGATTGCGCACTTCTCAATATCCCCGAGAAACAGACCCTGGCGATCAGCACTGATACGCTGGTGGCGGGTAACCATTTCCTCCCTGATATCG  >  AP012306/343261‑343554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`