Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 1,160,178 C→T A388T (GCG→ACG)  sapA ← predicted antimicrobial peptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,160,1780CT100.0% 874.0 / NA 221A388T (GCG→ACG) sapApredicted antimicrobial peptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (98/123);  total (98/123)

Only 100 of 228 total aligned reads displayed.

AACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCC  >  AP012306/1160031‑1160323
                                                                                                                                                   |                                                                                                                                                 
aaCCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCt                                                                                                                                                 <  2:3845570/150‑1 (MQ=255)
 aCCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTg                                                                                                                                                >  2:1443780/1‑150 (MQ=255)
  ccGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGc                                                                                                                                               >  2:2103100/1‑150 (MQ=255)
  ccGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGc                                                                                                                                               <  2:1702444/150‑1 (MQ=255)
     gATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGaa                                                                                                                                            >  2:2961025/1‑150 (MQ=255)
      aTAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAAc                                                                                                                                           <  1:866296/150‑1 (MQ=255)
         aCGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTGCCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACgtg                                                                                                                                        >  2:3408494/1‑150 (MQ=255)
          cGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACgtgt                                                                                                                                       <  1:981344/150‑1 (MQ=255)
           gTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGtgtg                                                                                                                                      >  2:1780390/1‑150 (MQ=255)
            tCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTgg                                                                                                                                     >  1:124069/1‑150 (MQ=255)
             cAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTggg                                                                                                                                    >  2:2733541/1‑150 (MQ=255)
              aGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGc                                                                                                                                   >  1:3623673/1‑150 (MQ=255)
              aGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGc                                                                                                                                   >  2:827059/1‑150 (MQ=255)
                aTCATGGTTCATACCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCAc                                                                                                                                 <  2:3279078/150‑1 (MQ=255)
                 tCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCAcc                                                                                                                                >  1:3202763/1‑150 (MQ=255)
                    tGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCcac                                                                                                                             >  2:1151741/1‑150 (MQ=255)
                        tCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGtt                                                                                                                         <  1:1563160/150‑1 (MQ=255)
                         cATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGttt                                                                                                                        >  1:83222/1‑150 (MQ=255)
                          atatCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTc                                                                                                                       <  1:2304530/150‑1 (MQ=255)
                          atatCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTc                                                                                                                       <  1:3111192/150‑1 (MQ=255)
                           tatCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCa                                                                                                                      >  1:2847908/1‑150 (MQ=255)
                            atCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAg                                                                                                                     <  2:1754288/150‑1 (MQ=255)
                            atCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAg                                                                                                                     >  2:2773655/1‑150 (MQ=255)
                            atCCATCAAACGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAg                                                                                                                     <  1:3002919/150‑1 (MQ=255)
                             tCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGc                                                                                                                    >  1:3690392/1‑150 (MQ=255)
                             tCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGGTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACGGATTCAGc                                                                                                                    >  2:1121420/1‑150 (MQ=255)
                               cATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGt                                                                                                                  <  2:3935101/150‑1 (MQ=255)
                                  cAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGCCTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTaa                                                                                                               >  1:2528976/1‑150 (MQ=255)
                                   aaCCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTaaa                                                                                                              <  2:2421717/150‑1 (MQ=255)
                                   aaCCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTaaa                                                                                                              >  1:3946723/1‑150 (MQ=255)
                                       gcgcCTCCTTAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAAtttt                                                                                                          <  2:1625623/150‑1 (MQ=255)
                                       gcgcCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAAtttt                                                                                                          <  2:1881150/150‑1 (MQ=255)
                                       gcgcCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAAtttt                                                                                                          <  1:430009/150‑1 (MQ=255)
                                         gcCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTcc                                                                                                        >  2:562764/1‑150 (MQ=255)
                                            tcctGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTAAAATTTTCCAGc                                                                                                     <  2:4336131/150‑1 (MQ=255)
                                               tGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGcccc                                                                                                  >  1:4105875/1‑150 (MQ=255)
                                               tGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGcccc                                                                                                  >  2:313134/1‑150 (MQ=255)
                                               tGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGcccc                                                                                                  >  1:1304928/1‑150 (MQ=255)
                                               tGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGcccc                                                                                                  >  1:2414305/1‑150 (MQ=255)
                                               tGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGcccc                                                                                                  <  2:2453739/150‑1 (MQ=255)
                                                gAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCa                                                                                                 <  1:3220412/150‑1 (MQ=255)
                                                 aaaGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCaa                                                                                                <  2:2050727/150‑1 (MQ=255)
                                                   aGCGACCTTCTGCCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACg                                                                                              <  1:1688743/150‑1 (MQ=255)
                                                      gACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACt                                                                                           >  1:2143060/1‑150 (MQ=255)
                                                       aCCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACtt                                                                                          >  1:3803466/1‑150 (MQ=255)
                                                         cTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCa                                                                                        >  1:3423150/1‑150 (MQ=255)
                                                          ttCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCaa                                                                                       >  1:123457/1‑150 (MQ=255)
                                                           tCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAAc                                                                                      >  1:470550/1‑150 (MQ=255)
                                                               ccGGCACAATCCCCCCTTTTACGCCACCCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGtt                                                                                  <  2:3076003/150‑1 (MQ=255)
                                                               ccGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGtt                                                                                  <  2:2320761/150‑1 (MQ=255)
                                                                cGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTc                                                                                 <  2:1542788/150‑1 (MQ=255)
                                                                cGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTc                                                                                 <  2:3116754/150‑1 (MQ=255)
                                                                  gCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTcgc                                                                               >  1:225995/1‑150 (MQ=255)
                                                                   cacaATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTcgcg                                                                              <  2:668396/150‑1 (MQ=255)
                                                                    acaATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTcgcgc                                                                             >  1:3077262/1‑150 (MQ=255)
                                                                    acaATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTcgcgc                                                                             <  2:3520341/150‑1 (MQ=255)
                                                                       aTCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGAt                                                                          >  2:2360762/1‑150 (MQ=255)
                                                                         caccacTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGAttt                                                                        <  2:325769/150‑1 (MQ=255)
                                                                          accacTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTc                                                                       <  1:637545/150‑1 (MQ=255)
                                                                             acTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGcc                                                                    >  1:3823898/1‑150 (MQ=255)
                                                                               ttttACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCGGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCgg                                                                  >  2:3347186/1‑150 (MQ=255)
                                                                                 ttACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGAt                                                                >  2:3563617/1‑150 (MQ=255)
                                                                                  tccGCCAACCCGAGCATTATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCAAACGACTTCAACTGTTCGCGCGATTTCGCCGGAtt                                                               <  2:3031034/148‑1 (MQ=255)
                                                                                  tACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGAtt                                                               <  1:3682920/150‑1 (MQ=255)
                                                                                   aCGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTg                                                              <  2:1310268/150‑1 (MQ=255)
                                                                                    cGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGt                                                             >  2:1586098/1‑150 (MQ=255)
                                                                                     gCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTa                                                            <  1:1207961/150‑1 (MQ=255)
                                                                                        aaCCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTc                                                         <  2:2039410/150‑1 (MQ=255)
                                                                                           cTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGt                                                      <  2:2184988/150‑1 (MQ=255)
                                                                                            tGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTa                                                     >  2:3900887/1‑150 (MQ=255)
                                                                                             gcgcCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTaa                                                    >  2:3160316/1‑150 (MQ=255)
                                                                                                ccATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGGGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAAttt                                                 >  2:3482826/1‑150 (MQ=255)
                                                                                                   tatCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAg                                              >  2:2605792/1‑150 (MQ=255)
                                                                                                   tatCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAg                                              >  2:1358682/1‑150 (MQ=255)
                                                                                                   tatCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAg                                              <  1:3881451/150‑1 (MQ=255)
                                                                                                    atCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGc                                             >  1:1987757/1‑150 (MQ=255)
                                                                                                        tccTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCg                                         <  2:3976173/149‑1 (MQ=255)
                                                                                                          cTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGtt                                       <  2:806077/150‑1 (MQ=255)
                                                                                                               tCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCAt                                  >  1:2472205/1‑150 (MQ=255)
                                                                                                                cAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATa                                 >  1:1025298/1‑150 (MQ=255)
                                                                                                                cAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATa                                 >  1:564612/1‑150 (MQ=255)
                                                                                                                 aGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAg                                >  2:2501928/1‑150 (MQ=255)
                                                                                                                 aGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAg                                <  1:1910192/150‑1 (MQ=255)
                                                                                                                   ttCGGCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGc                              <  1:823766/150‑1 (MQ=255)
                                                                                                                       gCAGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCAc                          <  2:2249726/150‑1 (MQ=255)
                                                                                                                         aGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGa                        >  1:1577550/1‑150 (MQ=255)
                                                                                                                         aGTTTTCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGa                        <  2:300505/150‑1 (MQ=255)
                                                                                                                           ttttCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAgg                      >  2:1426991/1‑150 (MQ=255)
                                                                                                                            tttCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGgc                     >  1:4120309/1‑150 (MQ=255)
                                                                                                                            tttCAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGgc                     <  2:4170276/150‑1 (MQ=255)
                                                                                                                               cAGTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGgcgcg                  >  2:884697/1‑150 (MQ=255)
                                                                                                                                 gTGGACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGcgcgcg                >  2:2075081/1‑150 (MQ=255)
                                                                                                                                    gACTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTa             <  1:2646819/150‑1 (MQ=255)
                                                                                                                                     aCTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTaa            <  2:4272662/150‑1 (MQ=255)
                                                                                                                                      cTGGGGTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTaaa           >  2:624550/1‑150 (MQ=255)
                                                                                                                                          ggTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATag       <  2:1468577/150‑1 (MQ=255)
                                                                                                                                           gTTCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATaga      <  2:524460/150‑1 (MQ=255)
                                                                                                                                             tCCACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAgg    <  1:3757778/150‑1 (MQ=255)
                                                                                                                                               cACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGgcc  <  1:2929226/150‑1 (MQ=255)
                                                                                                                                               cACGTCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGgcc  <  1:1465907/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AACCGGATAACGTCAGATCATGGCTCATATCCATCAACCGCGCCTCCTGAAAGCGACCTTCTACCGGCACAATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTCCAGCCCCAACGACTTCAACTGTTCGCGCGATTTCGCCGGATTGTATTCAGTAATTTTAGCCTCGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCC  >  AP012306/1160031‑1160323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑